cd133 cells from frozen leukapheresis products

... vitro derived sperm cells and oocytes from ES cells will be useful to study early meiosis events (Hubner et al 2003) Additionally, technology for deriving germ cells from human ES cells will permit ... survival and has a role in maintenance of reproductive germ cells The knockout leads to progressiveloss of germ cells, Sertoli cells and Leydig cells and cause extensive testicular degradation A protein ... supported by Sertoli cells in the seminiferous tubules of the testis, which are non-dividing diploid cells that form the blood-testis barrier The entire process from spermatogonial stem cells to mature...

Ngày tải lên: 25/10/2013, 21:20

... Copyright 1996 Scientific American, Inc AP/WIDE WORLD PHOTOS Hormonal Relief from Alzheimer’s Evidence from animal research and from studies on postmenopausal women suggests that estrogen replacement ... stop anybody from storing a Web site on a disk drive DAVID SUTER Television by Any Other Name located in France But that wouldn’t keep the user from accessing the same material from a computer ... stimulate the production of tumor-specific white blood cells, so that there are more of them available to destroy the cancerous cells; these white blood cells could then be put back inside the body to...

Ngày tải lên: 12/05/2014, 15:15

... 1A9 cells, and taxolresistant PTX10 cells that are derived from the 1A9 cell line [39] Unfortunately, the mechanism of taxol resistance in PTX10 is not by Aurora-A overexpression Rather, PTX10 cells ... of Aurora kinases results in cell death Cells were treated for 96 hours with differing doses of VE-465 (A) PTX10 cells (B) 1A9 cells Following treatment cells were harvested, fixed and stained ... in PTX10 cells This could be because a very high percentage of cells are undergoing apoptosis at high doses, or possibly due to the inherent nature of the resistance of PTX0 cells PTX10 cells harbor...

Ngày tải lên: 18/06/2014, 15:20

... EOC cells Figure vation in4Type I EOC cells Differential effect of ARRY-520 and Paclitaxel on NF-κB actiDifferential effect of ARRY-520 and Paclitaxel on NFκB activation in Type I EOC cells Cells ... injection of R182 cells in mice (which is > 90% CD44+ by flow cytometry pre-injection) resulted in s.c tumors containing < 10% CD44+ positive cells [14] The differentiation of the R182 cells from Type ... RPMI plus 10% fetal bovine serum (Gemini Bio -Products, Woodland, CA) Primary EOC cell lines were isolated from malignant ovarian ascites or explanted from ovarian tumors and cultured as previously...

Ngày tải lên: 18/06/2014, 15:20

... vector-transduced (EV) cells were cultured in serum-free DMEM/F12 media for 48 hours Cells were disengaged from the culture flasks using CellStripper solution (Cellgro, Herndon, VA) and × 104 cells were ... migration assay P, ST6, and EV cells were cultured in serum-free media for 48 hours and disengaged from the culture dishes using CellStripper solution 2.5 × 10 5cells were then seeded into the ... binding, cells were also plated onto dishes pretreated with dBSA alone Cells were allowed to adhere for 30 minutes at 37°C, and then samples were washed gently with PBS The remaining adherent cells...

Ngày tải lên: 20/06/2014, 07:20

... treatment of cells with any of the drugs alone (Figure 4) These results suggest that the pretreatment of cells with genistein sensitized ovarian cancer cells, especially the drug-resistant cells to ... general effects on all rapidly dividing cells It is important that we use targeted agents that discriminate between cancer stem cells and normal stem cells One such agent which has been studied ... solution Cell Viability Assay Paired cells A2780 and C200 were chosen for this study Cells (2–5 × 104) were seeded in a 96-well culture plate and incubated overnight Cells were treated with varying...

Ngày tải lên: 20/06/2014, 07:20

... obtained from Dr D Connolly, OVCA-429, OVCA-432, and OVCA-433 were obtained from Dr R Bast, Jr., IGROV-1 and OVCAR-8 Page of were obtained from Dr W Cliby, SKOV-6 and SKOV-8 were a obtained from ... which was purchased from GibcoBRL Cetuximab was obtained from Bristol Myers Squibb, trastuzumab was obtained from Genentech, and erlotinib, gefitinib, and lapatinib were obtained from Chemitek Bovine ... antibodies were purchased from Santa Cruz Biotechnologies Anti-HER2 (Ab-1) antibody was purchased from NeoMarkers, Inc Function-blocking anti-HER3 antibody (H3.105) was purchased from Upstate Biologicals...

Ngày tải lên: 20/06/2014, 07:20

... Valley, PA) Colony formation assay For this assay, cells were seeded at 500 cells per 100 mm culture dish and allowed to attach overnight The cells were treated with curcumin or cisplatin or with ... A2780CP cells, 50,000 cells were plated in well chamber slides and after 24 hrs the media was replaced with PLGA NPs (20 μg of FITC) diluted in media After hrs incubation with FITC-PLGA NPs, cells ... dose (Gy) Figure Curcumin pre-treatment sensitizes cells to radiation exposure and reduces the clonogenic potential of A2780CP cells A2780CP cells were treated with the indicated amounts of curcumin...

Ngày tải lên: 20/06/2014, 07:20

... significant (P < 0.05) increase of cells in early apoptosis from 2.2% ± in vehicle-treated cells to 10.7% ± after a 24 hour treatment (10 μM) At a higher dose of 40 μM, the cells in early apoptosis significantly ... percentage of cells actively undergoing apoptosis according to the manufacturer's instructions Briefly, ovarian carcinoma cells were seeded overnight in wells plates at a density of × 105 per well Cells ... manufacturer's instructions Briefly, ovarian carcinoma cells were seeded in 96-well plates at a density of × 104 cells/ well After 24 h, cells were treated with different concentrations of T0901317...

Ngày tải lên: 20/06/2014, 07:20

... leads to enrichment of the SP population in ovarian cancer cells Increased number of CD133- positive cells in MUC4 overexpressed SKOV3 cells CD133 is a cell surface antigen which was recognized as ... FITC-conjugated CD133 cells to analyze the percentage of the cancer stem cell population Interestingly, MUC4transfected SKOV3 cells showed a 0.1% increased CD133 population as compared to the control cells ... ovarian cancer cells consist of a heterozygous population of cells having different phenotype Side population and Non-side population in MUC4 transfected SKOV3 cells Recently, cancer stem cells have...

Ngày tải lên: 20/06/2014, 07:20

... sequences are available from the authors Antibodies and Immunoblotting All the antibodies used for this work were obtained from commercial sources AntiABCB1 was purchased from GeneTex Anti-SPOCK2 ... GeneTex Anti-SPOCK2 and anti-CCL26 were obtained from R&D Systems Anti-PRSS8 and anti-MSMB were obtained from Novus Biologicals Anti-GAPDH was purchased from Abcam Immunoblotting was performed as previously ... OV90D and OV90P cells, while the increase in cisplatin-resistant OV90C cells was approximately 15-fold (Figure 3A) Similarly XAGE1D expression was also increased 1,000-fold in OV90P cells compare...

Ngày tải lên: 20/06/2014, 08:20

... during the transition from maternal gene activity is consistent with a series of observations made in several vertebrate models ([2] and references therein) The picture emerging from the study by ... for mouse TBP2 in female germ cell development The conclusions drawn from studies in frog oocytes [5] match those stemming from expression analysis and recent http://jbiol.com/content/8/11/97 ... regula Oogenesis Meiotic maturation Fertilized egg/ zygote Zygotic gene expression Frog 4000 cells Mouse cells Figure Regulation of TBP and TBP2 during oogenesis and the early stages of embryogenesis...

Ngày tải lên: 06/08/2014, 19:21

... older than age 38 [16] 1.E+06 Number of germ cells difficult to establish Cytochemical methods have suggested FGC numbers varying from 45 cells [2] to 193 cells [3] On the other hand, genetic analysis ... in germ cells of the mouse, but also in somatic cells that surround these germ cells [21] Recent description of cytochemical markers that are more specifically restricted to the germ cells confirms ... and weeks Using morphological clues to distinguish germ cells from somatic cells, these authors were able to establish the number of germ cells per tissue sample throughout this period We used...

Ngày tải lên: 13/08/2014, 23:20

... intracellular fragment derived from TRA-2 (TRA-2ic; see Figure 1) can bind directly to TRA-1 [14,15] Furthermore, mutations that disrupt this interaction cause all germ cells to choose oogenesis [14-17] ... elegans has two pathways that transmit information from TRA-2 to the nucleus, the requirement for the fem genes might have arisen recently from a subsidiary role like that in C briggsae If so, ... They had suspected that this might be so, as fog-2 seemed to have evolved recently in C elegans from a duplicated F-box gene [23] And Nayak et al [22] also showed that the partner of FOG-2 in...

Ngày tải lên: 14/08/2014, 16:21

... germ cells and inner germarial sheath cells (IGS cells) during Drosophila oogenesis Two aspects of signals are studied: one, signals from early germ cells to IGS cells and the other, signals from ... early germ cells and IGS cells in adult Drosophila germaria As such, two aspects will be investigated as listed below: I) signals from early germ cells to IGS cells; II) signals from IGS cells to ... stem cells and adult stem cells The embryonic stem cells are totipotent, and this means that they can differentiate to all types of cells, which constitute an entire organism Adult stem cells...

Ngày tải lên: 14/09/2015, 08:42

... stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal stem cells ... reagent were purchased from Invitrogen (Carlsbad, CA, USA) M-MLV reverse transcriptase was obtained from Promega (Madison, WI, USA) and Power SYBR Green PCR Master Mix was purchased from Applied Biosystems ... IL-1b and TNF-a from hypoxia ⁄ SD-stimulated MSCs, as well as the significant anti-inflammatory effects of MSCs, expression and secretion of the anti-inflammatory cytokine IL-10 by these cells was investigated...

Ngày tải lên: 18/02/2014, 04:20

... other blood cells including CD281 cells and CD31 cells (data not shown) The antibody was also used to probe Western blots of extracts from several cell lines and purified peripheral blood cells The ... adherent transfected COS7 cells, a binding assay with adherent endothelial cells was also performed HUVEC cells (ATCC) were plated and grown to confluence in 96-well plates COS7 cells were transfected ... 48 and 60 h post-transfection Blood cells and cell lines were labeled with calcein-AM (5 mL per 108 cells) for 30 at 37 8C Red blood cells, mixed white blood cells, Ramos and Daudi (B-cell lines),...

Ngày tải lên: 24/03/2014, 04:21

... 10 expression was severely reduced in tumors derived from ADAM 10-RNAi cells compared to tumors derived from parental or mock-transfected cells (Figure 6B, D) These data again supported the argument ... for each cell group Then, the cells were incubated for 24 h in a humidified atmosphere of 5% CO2 at 37°C Cells were fixed with methanol and stained with Giemsa Cells on the upper surface of the ... and SACC-83 cells (a), (b) A Matrigel transwell invasion assay was used to test the ability of SACC-LM and SACC-83 cells to invade the filter membrane (c), (d) Overview of lung tissues from mice...

Ngày tải lên: 18/06/2014, 16:20