cck pyy and pp the control of energy balance
Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... lower panels show the binding isotherm and the best-fit curve according to the three sequential binding sites model BIAcore analysis of the GS–TnrA interaction The results of the immunoprecipitation ... specific for TnrA The GlnK–TnrA complex appeared to be quite stable, as revealed by the very slow dissociation of the complex following the injection phase (Fig 2A) In the course of the measurements, ... PII trimer The upper panel shows the raw data in the form of the heat effect during the titration of 25 lM GlnK solution (trimer concentration) with ATP (titration from 2.1 to 73.5 lM) The lower...
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Báo cáo khoa học: Crystal structure of basic 7S globulin, a xyloglucanspecific endo-b-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-b-glucanase doc
... (C) The overall structure of the Bg7S protomer is shown by a ribbon representation The structure of the A molecule is shown as an example The N-terminus and C-terminus are labeled The a-chain and ... symmetry (Fig 1A) The N-terminal moieties of the b-chains of the C and D molecules protrude into the AB dimer (Fig 1A), whereas the corresponding regions of the A and B molecules are disordered We have ... aculeatus [19] We further investigated the activity of the GH11 xylanase ANXY in the presence of Bg7S (Fig 4C) As expected, Bg7S did not affect the activity of ANXY Even in the presence of leginsulin,...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
... within the middle segment of the a-, b- and c-chains Hence, no further classification of the sponge protein to any of the three vertebrate chains can be made In the sponge sequence, besides the first ... performed The results revealed that the expression level of the glucanbinding protein at the beginning of the experiments is low However, after only day of incubation in the presence of 10 lgÆmL)1 ... the origin of most secondary metabolites identified in sponges is not clear The understanding of the pathways which result in the synthesis of these compounds in sponges is crucial for their sustainable...
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Báo cáo khoa học: The ubiquitin-like protein monoclonal nonspecific suppressor factor b conjugates to endophilin II and regulates phagocytosis potx
... Mobilities of the 95 and 62 kDa MNSFb adducts and the molecular mass standards (kDa) are indicated to the right and left of the figure, respectively trypsin Selected peptides from the 62 kDa band were ... products of endophilin II and MNSFb The fourth column indicates the numbers of the first and last amino acid of the indicated endophilin II and MNSFb peptides, whereas the fifth shows the corresponding ... between the C-terminal glycine of MNSFb and the lysine of endophilin II The 62 kDa MNSFb adduct was digested by trypsin and subjected to MALDI-TOF MS analysis The data in the first column are the...
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Báo cáo y học: "Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain"
... (L5) and L6 (21) The catheter was chronically implanted and the external portion of the catheter was protected according to Milligan’s method (22) Intrathecal delivery of siRNA Rats were randomly ... (29) One of the potential advantages of RNAi technologies is the ability to design precisely targeted therapeutics for almost any gene, regardless of the function of the gene product, whether that ... confirmed at the mRNA and protein levels TNF-α and IL-1β were up-regulated in the dorsal spinal cord tissues of CCI rats, and there were no significant differences in TNF-α and IL-1β between the http://www.medsci.org...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... structures, where the aliphatic portion of the side chain fits into the hydrophobic alcove and allows the charged head group access to the solvent on the other side The extended side chain of Arg206 allows ... carbonyl and amide backbone groups The one exception to this is the location of the amide group of Lys204 at P3, where there is a break in the main-chain density at the 2.8r map level The corresponding ... primarily occurs by the formation of a hydrogen bond between the side-chain hydroxyl group of Tyr205 and the importin-a main chain at the C-terminus of the ARM1 H3 helix The hydrogen bond is...
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Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx
... on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and treatment of these conditions Suppression ... significant decreases in the expression of PPARd and PGC-1a in skeletal muscle in ANGPTL6 ⁄ AGF null mice, and increases in the expression of PPARa, PPARd and PGC-1a in skeletal muscle of ANGPTL6 ⁄ AGF ... regulates energy expenditure, which is mediated by PPARa, PPARd, PPARc and their coactivators, peroxisome proliferator-activated receptor-c (PPARc) coactivator (PGC)-1a and PGC-1b, in response to energy...
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Tài liệu Báo cáo khoa học: Mitochondrial chaperone tumour necrosis factor receptor-associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening docx
... TRAP1-siRNA or control vector by the addition of adenovirus to the cell culture at a multiplicity of infection of 10 After days of infection, the cells were serum starved for 12 h and then treated ... identified by the yeast twohybrid system as a novel protein that interacted with the intercellular domain of the type tumour necrosis factor receptor [17] On the basis of the sequence of the homologue, ... 10 lL of CCK- 8 solution and 100 lL of DMEM ⁄ F12 medium were added to each well, and the cells were incubated at 37 °C in the dark for h in accordance with the manufacturer’s instructions The value...
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Báo cáo khoa học: Human lactoferrin activates NF-jB through the Toll-like receptor 4 pathway while it interferes with the lipopolysaccharide-stimulated TLR4 signaling potx
... or other antibacterial molecules [6] The LPS-binding activity of LF may account for the other properties of this protein in the modulation of the inflammatory process [3] The stimulation of mammalian ... after days of culture were used as macrophages The protocol of animal preparations for the experiment was approved by the Ethics Committee of our institute Preparation of the nuclear and cytoplasmic ... for the indicated periods of time, and nuclear and cytoplasmic extracts were prepared as described in the Materials and Methods The IKK activities in the cytoplasmic extracts were determined The...
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Báo cáo khoa học: A unique vertebrate histone H1-related protamine-like protein results in an unusual sperm chromatin organization pot
... independently used as the source of new SNBP models The higher DNAbinding affinity of the SNBPs of the P and PL types would explain why they are often found in organisms at the tips of the phylogenetic ... described [74] The position of the replication-independent somatic H1 histones is indicated in the right margin of the tree and the group including the protamine-like proteins and the germinal ... identity of the members of the histone H1 family appears to be given by the specific sequence of their characteristic winged helix domain (Fig 2), most of their tissue specificity resides in the intrinsically...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
... pairs of hydrogen bonds are formed in the active site of hTRXL-N (Fig 4), accounting for the compactness and stability of the active site The H-bond length between the carbonyl oxygen of Cys34 and ... wrapped around the protrusion of the reactive Cys32 in a crescent-shaped groove However, the orientation of the Ref1 peptide is opposite to that of the target peptide from NFjB The ability of hTRX to ... case of TRX Furthermore, the loss of intermolecular disulfide-bonds and the disbandment of the hydrophobic patch may also obstruct the dimer formation (Fig 6) The1 4 residues in the dimer interface...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... isolation and sequencing of the fulllength TgDRE cDNA The comparison of cDNA and genomic DNA nucleotide sequences allowed the determination of the exact position of the two introns which were 780- and ... none were found in all of the sequences of the SF45 family The SF45, G-patch and RRM motifs are shown boxed The total lengths of the proteins are indicated at the end of each sequence ACKNOWLEDGEMENTS ... to the DNA repair enzyme (DRT111) of Arabidopsis thaliana [9,10] Here, we report on the isolation of the gene locus, full-length cDNA and the functional analysis of the corresponding protein of...
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Báo cáo khoa học: A role of monocyte chemoattractant protein-4 (MCP-4)/CCL13 from chondrocytes in rheumatoid arthritis doc
... asthma and atopic dermatitis [14–17] In the present study, we further determined the role of MCP-4 ⁄ CCL13 in RA pathogenesis We investigated the role of several stimuli on the expression of MCP-4 ... one of the key molecules in the pathogenesis of RA, we studied the association of the MCP-4 ⁄ CCL13 gene with RA susceptibility We selected two SNPs in MCP-4 ⁄ CCL13 (T887C and rs159313), for the ... cent of them were rheumatoid factor positive They were mostly females (82.6%), and the mean age of the patients was 57.6 years (range: 19–85 years) Four hundred and fifty-five population-based control...
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Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx
... domain of TSG101 [14] The crystal 3D structures of the N-terminal domain (designated Bro1 domain) of yeast Bro1 [15] and that of human Alix [16] have revealed folded cores of 360 residues The ... Strep–BroxWT and Strep–BroxC408S and with the comparable N-terminal regions containing the Bro1 domain of Alix (Strep– Alix1)423) and HD-PTP (Strep–HD-PTP1)431) (Fig 2A) On the other hand, FLAG–RabGAPLP ... palmitoylation of Cys immediately upstream (2–6 residues) of farnesylated Cys of H-Ras, N-Ras and K-Ras4A, and a stretch of basic residues upstream of the farnesylated Cys186 of K-Ras4B [34–36]...
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
... The first 17 residues of 4E-BP1 contain the RAIP motif To assess whether removal of the RAIP motif accounts for the reduced binding of raptor to the 18– 117 fragment, we compared the binding of ... that the loss of raptor binding on removal of the first 17 residues may be accounted for simply by the loss of the RAIP motif We therefore also tested the binding of raptor to full-length 4E-BP1 and ... define the RAIP motif [25] The use of the rat protein also has the advantage that there is no cross-reactivity of the (P)Ser64 antibody with other sites, which is a complicating feature of the human...
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Báo cáo khoa học: Identification and expression of the first nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt
... present in the other members of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of a second heparin-binding domain [3,4,13,26] Comparative analysis of the Xenopus ... to the generation of APLP1 and preAPP/APLP2 lineages (rather than to the APP and preAPLP separation as found by our analysis), and the second duplication event would have caused the splitting of ... the Swiss-Prot database Accession numbers were: human APP (HsAPP), P05067; murine APP (MmAPP), P12023; chicken APP (GgAPP), Q9DJG7; Xenopus laevis APP gene A (XlAPPA), Q98SG0 and gene B (XlAPPB),...
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Báo cáo khoa học: Glycogen synthase kinase 3b and b-catenin pathway is involved in toll-like receptor 4-mediated NADPH oxidase 1 expression in macrophages ppt
... types of mice, with LPS, and compared the Nox1 mRNA expression using RT-PCR The effects of LPS were strong in the BMDM of C3H ⁄ HeN, but relatively weak in the BMDM of C3H ⁄ HeJ (Fig 1C) The data ... responses and the development of atherosclerosis [17] Therefore, the regulation of Nox1 mRNA expression may be a potential therapeutic target for FEBS Journal 277 (2010) 2830–2837 ª 2010 The Authors ... macrophages, and that the ROS produced are part of an important process controlling foam cell formation [24] Glycogen synthase kinase (GSK3)b and the b-catenin pathway are crucial regulators in the balance...
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Báo cáo khoa học: Identification of an osteopontin-like protein in fish associated with mineral formation pot
... osteopontin To test this hypothesis and to determine whether osteopontin and OP-L protein have retained the same function in the course of evolution, the gene and peptide structures of both proteins were ... observed in their amino acid content in acidic clussequences and the proposed affiliation of these ters The gene expression pattern further supports the sequences with mammalian osteopontin The in ... SSH (a, b and c in Fig 1A) and used in a combination of RACE and standard PCR amplifications to amplify overlapping fragments (d, e and f in Fig 1A) A 2138-bp sequence corresponding to the full-length...
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Báo cáo khoa học: Dynamin-like protein-dependent formation of Woronin bodies in Saccharomyces cerevisiae upon heterologous expression of a single protein pdf
... with respect to the plane of the section The short dimension fits with the measurement of the en face view of the hexagonal structures In some rare cases, peroxisomes with areas of distinct electron ... HEX1-expressing strains for the appearance of Woronin-body-like structures Furthermore, we determined whether the formation of Woronin bodies from peroxisomes shares components of the peroxisomal fission ... analyzed by immunoblotting for the presence of HEX1, the peroxisomal marker proteins Pex13p and Cta1p The detection of HEX1 and Cta1p in the pellet fraction indicated that the vps1Ddnm1D mutant remained...
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Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt
... EC0 and ECU represent the amount of acyl-enzyme complex formed in the absence and presence of the unlabeled antibiotic, respectively, and CU and CL are the concentrations of the unlabeled and ... between the infected female and male, with the vagina having a pH range of 4.27.5 and the male urethra having a pH of 6.28.4 The pH dependence of NG PBP (pKa values of 6.9 and 10.1) therefore ... obtained, in these cases due to the lack of apparent substrate saturation Since the possibility of substrate inhibition also exists for the other two substrates, Ac2-KAA and Ac2-KA-D-Lac, the values...
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