ccaat enhancer binding protein b c ebpb

... TgCgCTTgCAgAgATTAAAA AgCCCTgTATTCCgTCTCCT IL4 NM_021283, CgAggTCACAggAgAAgggA AAgCCCTACAgACgAgCTCACT Actin NM_007393.3 CAACgAgCggTTCCgATg gCCACAggATTCCATACCCA Rn18s NR_003286.2 gTAACCCgTTgAACCCCATT ... TTATgAgATgTgCCCTCTgC CCACCTAAggggAACAgTgA IL 1b IL6 tgtgTgaaGaAAgaa gTttCCAATcagccc -16/-31 -173/-188 TCAggAACAgTTgCCATAgC AgACCTATACAACggCTCCT gTTgTgATTCTTTCgATgCT ggAATTgACTATCgTTCTTg TNFa agggTTtgGaAAgtt ... TggTgTgTgACgTTCCCATTA gCATTggAAgTgAAgCgTTTC CAgCACgAggCTTTTTTgTTg IL6 NM_031168.1 CCAgTTTggTAgCATCCATC CCgCAgAggAgACTTCACAg TNFa NM_013693.2 TgATCCgCgACgTggAA ACCgCCTggAgTTCTggAA TGFb1 NM_011577.1 TgCgCTTgCAgAgATTAAAA...

Ngày tải lên: 19/06/2014, 22:20

... with the C/ EBPa expression vector (Fig 1C) C/ EBP -binding < /b> activity in rat liver has been described as involving both C/ EBPa and C/ EBPb However, when pMSV /EBPb was assayed in cotransfection experiments ... posthoc Fig Transactivation of CYP3AT81Luc constructs with vectors pMSV/EBPa or pMSV /EBPb in the CHO cell line Cotransfections were carried out using 0.5 lg of CYP3AT81Luc and increasing concentrations ... lucif, luciferase cDNA (B) Cotransfection of CYP3AT81Luc with 0.15 lg of pMSV/EBPa (C) Cotransfection of p0.8T81Luc with increasing concentrations of pMSV/EBPa or pMSV /EBPb The normalized luciferase...

Ngày tải lên: 17/03/2014, 09:20

... AATCCCGGGTTACTTTAGTTTATCTTTGCCG GAATTATCTTTAGCTCTAGCTATTGATCC GGATCAATAGCTAGAGCTAAAGATAATTC GCAGAATTCGTCGGCTTGAAATACGCTG AATGGATCCTTACTTTAGTTTATCTTTGCCG CCCAAGCTTGATGATGTCAGC CCCAAGCTTGAACGCCTTCATAGTGTC BamHI ... AATCCCGGGTTACTTTAGTTTATCTTTGCCG GGGGGATCCGGTACAGATGTAACAAATAAAG ATTCCCGGGTAATTTTTCCAAGTTAAATTACTTG GGGGGATCCGGTACAGATGTAACAAATAAAG CTCCCCGGGCTATTGAATATTAAATATTTTGCTAA CCCGGATCCTATTTAGGTGGAGTTAGAGATAAT AATCCCGGGTTACTTTAGTTTATCTTTGCCG ... rFnBPB309–480 F rFnBPB309–480 R rFnBPB163–480 NF F rFnBPB163–480 NF F FnBPB(–142–480) F FnBPB(–142–480) R FnBPB(388–980) F FnBPB(388–980) R CGGGGATCCGCATCGGAACAAAACAATAC AATCCCGGGTTACTTTAGTTTATCTTTGCCG...

Ngày tải lên: 06/03/2014, 00:20

... Fn BR B Fn GST B Fn PB BP -1 B Fn -2/3 BP Fn B- 4 BP Fn B- 5 BP Fn B- 6 BP Fn BBP Fn B- 8 B Fn PBBP Fn B- 1 BP B1 1 0.0 D BR G A ST Fn BP Fn A-1 BP Fn A-2 BP Fn A-3 BP Fn A-4 BP Fn A-5 BP Fn A-6 BP ... Authors Journal compilation ª 2010 FEBS G Provenza et al B BR G B ST Fn BP Fn B- 1 BP B Fn -2 BP /3 Fn B- 4 BP Fn BBP Fn B- 6 BP Fn B- 7 BP Fn BBP Fn B- 9 BP B Fn -1 BP B1 1 A 3.0 FnBPA and FnBPB from S aureus ... A-7 BP Fn A-8 BP Fn A-9 BP A Fn -1 BP A -1 Fn Fn BR B G ST B Fn PB BP -1 B Fn -2/3 BP Fn B- 4 BP Fn B- 5 BP Fn BBP Fn B- 7 BP Fn BB Fn PB BP -9 B Fn -1 BP B1 1 0.0 Fn A490 nm 2.5 Fn A 3.5 BR B G...

Ngày tải lên: 06/03/2014, 22:21

... of C4 BP (A) and model of C4 BP b- chain CCP1-2 (B) (A) C4 BP consists of seven identical a-chains and one b- chain Each chain is made up repeating complement control protein < /b> (CCP) domains The a-chains ... monoclonal antibodies, MoAb 15 and 44, against the recombinant wild-type C4 BP b- chain CCP1-2 These antibodies specifically recognized both the recombinant b- chain CCP1-2 and the full-length b- chain ... wild-type b- chain Ó FEBS 2003 The role of CCP2 of the C4 BP b- chain in binding < /b> of protein < /b> S (Eur J Biochem 270) 99 Fig Binding < /b> of MoAb 15 and 44 to C4 BP Microtiter plates were coated with either C4 BP...

Ngày tải lên: 17/03/2014, 09:20

... to a broad selection of insoluble carbohydrates (e.g ChbB, which binds both a- and b- chitin [21], and Chb3 from St coelicolor, which binds a-chitin, b- chitin, colloidal chitin and chitosan [22]), ... sequences of LlCBP33A (family 33 CBP of L lactis ssp lactis), ChbB (family 33 CBP from B amyloliquefaciens) and CBP21 (family 33 CBP from S marcescens) Fully conserved residues are shaded in black ... conducted for CBP21 from Serratia marcescens [17,20], ChbB [21] and Chb3 [22] from Streptomyces coelicolor, CHB1 from Streptomyces olivaceoviridis [23], CHB2 from Streptomyces reticuli [24], CbpD from...

Ngày tải lên: 18/02/2014, 08:20

... displaying a nucleotide binding < /b> site FEBS Lett 369, 22–26 ` 13 Bernier I & Jolles P (1984) Purification and characterization of a basic 23 kDa cytosolic protein < /b> from bovine brain Biochim Biophys Acta 790, ... amounts of A B C Fig Liposome -binding < /b> assay for IC and IC–CPY IC (A) or IC–CPY (B) , the final concentration of which was lM, was added to PCbased liposomes (0.5 mgÆmL)1 of PE ⁄ PC, PC, PS ⁄ PC, and PtdIns ... Fluorescence microscopic analyses of yeast cells producing IC–GFP (A) Representative fluorescence images S cerevisiae BY4741icD cells producing IC–GFP were labeled with the vacuolar membrane fluorescent...

Ngày tải lên: 19/02/2014, 05:20

... instructions The prey and bait plasmids used are described above Recombinant GSTmCtBP2 was produced in Escherichia coli strain BL-21, purified as described [28] and immobilized on glutathione beads ... N-terminal constructs are able to associate with cotransfected CtBP, lanes 1–3 (E) The reciprocal experiment with anti-CtBP serum precipitating the cotransfected Eos constructs (arrows) shown in (B) ... required for binding < /b> [31], and HDAC5 contains a related motif (PVELR) that is dispensable for CtBP contact [32] In the case of HDAC1, no canonical CtBP recognition sequences have been found [30]...

Ngày tải lên: 21/02/2014, 01:21

... Journal compilation ª 2010 FEBS Biophysical properties of hGBP5a ⁄ b and hGBP5ta M Wehner and C Herrmann Table Nucleotide binding < /b> dynamics of hGBP5a ⁄ b and hGBP5ta The dissociation constants were calculated ... possibly reflecting larger structural changes accompanying nucleotide binding < /b> The rate constants for nucleotide association and dissociation are both much smaller for hGBP5 compared to hGBP1, reflecting ... circles) or hGBP5ta (filled circles) To investigate the dynamics of nucleotide binding,< /b> constant concentrations of the nucleotides were mixed with increasing amounts of hGBP5a ⁄ b or hGBP5ta b...

Ngày tải lên: 15/03/2014, 10:20

... stickleback Fabp10a (St Fabp10a, BT027383), stickleback Fabp1 0b (St Fabp1 0b, Ensembl no ENSBACG00000002234), medaka Fabp10a (Me Fabp10a, Ensembl no ENSORLG00000014794) and medaka Fabp1 0b (Me Fabp1 0b, ... St Fabp10a Me Fabp10a 87 Zf Fabp10a 99 37 Cf Fabp10 Ig FABP10 34 Ch FABP10 6511 Lf Fabp10 Sa FABP10 18 56 75 To FABP10 Sh FABP10 Zf Fabp1 0b Zf Fabp1 0b St Fabp1 0b 41 74 100 Me Fabp1 0b Hu FABP6 ... FABP6 Zf Fabp6 Zf Fabp 1b 87 Fu Fabp1 99 Zf Fabp1a 39 Ch FABP1 58 Hu FABP1 47 56 Pi FABP1 Co FABP1 99 Mo FABP1 31 98 97 80 Ra FABP1 Mo FABP2 Ra FABP2 53 Hu FABP2 100 Zf Fabp2 Ch FABP2 Zf Fabp7a...

Ngày tải lên: 16/03/2014, 00:20

... (crabp1a; 3¢-RACE: 5¢-CCC AACTTCGCCGGCACCTGG-3¢, 5¢-TGAAAGCTCTCG GCGTAAAC-3¢; 5¢-RLM-RACE: 5¢-GAGCTTTCAGA AGTTCGTCG-3¢, 5¢-GAATCTCCACATGCGGTTTG-3¢) and a zebrafish genomic DNA sequence assembly (BX663612) ... genomic DNA from cell hybrids of the LN54 panel are: crabp1a: 5¢-TGGTTTGCACGCGGATTTAC-3¢, 5¢-GAAC GATGACTACAGCAATGG-3¢; crabp 1b: 5¢-GCAAATGT GAGCACTAAGTG-3¢, 5¢-CGGCCATCGACGGTCTC-3¢ The PCR conditions ... RT-PCR were designed based on cDNA sequences of the zebrafish crabp1a (5¢-TGAAAG CTCTCGGCGTAAAC-3¢, 5¢-GAAC GATGACTACAGC AATGG-3¢) and crabp 1b (5¢-GATTTGAAAGCAAGAGG GTC-3¢, 5¢-CTGCAAGTGCTGGAATATTC-3¢)...

Ngày tải lên: 16/03/2014, 22:20

... AF363957), chicken CRBPII (ChickCRBPII [43]), pig CRBPII (PigCRBPII; P50121), human CRBPII (HumanCRBPII; AAC50162), rat CRBPII (RatCRBPII; P06768), mouse CRBPIII (MouseCRBPIII; AA466092), rat CRBPI ... (5¢-TTCGCCACCCGTAAGATC-3¢), 0.4 lM antisense primer (5¢-AAACTCCTCTCCAATGACG-3¢), 0.2 mM Fig Nucleotide sequence of a cDNA clone coding for a zebrafish CRBPII The complete nucleotide sequence of ... (Eur J Biochem 269) Fig Amino-acid sequence alignment of zebrafish CRBPII with other CRBPs, CRABPs, and FABPs The amino-acid sequences of zebrafish CRBPII (ZbfshCRBPII; GenBank Accession number AF363957),...

Ngày tải lên: 17/03/2014, 11:20

... b- amino acids such as b- alanine or b- aminobutyric acid, c- aminobutyric acid and e-amino caproic acid were not activated Thus, the activating enzyme appears to be strictly speci c for b- lysine ... pg column equilibrated with buffer B, and 1-mL fractions were collected Fractions containing the b- lysine binding < /b> protein < /b> were combined and subjected to anion exchange chromatography on Resource ... (lRPC C2 /C1 8 column, Pharmacia) with acetonitrile/water gradients in the presence of tri¯uoroacetic acid Well resolved peaks were subjected to sequencing In the case of the b- lysine binding < /b> protein...

Ngày tải lên: 17/03/2014, 17:20

... ONXbaG ⁄ Bpro 1c, 5¢-GACTCTAGACTGGCCCACTATTTA CCCTCC-3¢; ON-GPBP- 5c, 5¢-CTCGATGCCAATTTCAA ATAGGGAA-3¢; ON-polj-2m, 5¢-GACAAGCCGCCCTG GAAAGCAGGCCC-3¢; ON-polj-3m, 5¢-GCAGCACAGC TGCATCCCTACCCCGCCCTCTC-3¢; ... 5¢-GGGTTCGGGAGAAGGC-3¢; ON-NFjB-1m, 5¢-GGGTTCGGGAGGAGGATCCCGAAGGC-3¢; ONNFjB- 1c, 5¢-GCCTTCGGGATCCTCCTCCCGAACCC-3¢; ON-SP1del -c, 5¢-CCCCCACTACGTCCCCCTCCC-3¢; ONPromMouse-F, 5¢-GACTCTAGAGGGAGCGTCGCGAG CCGCCGGGAG-3¢; ... ON-SP1- 3c, 5¢-CCA CTACTCCCCGCCCCGTCCCCC-3¢; ON-TATA-1m, 5¢-GA GGGGAGGGTAAATAGTGGGCCAG-3¢; ON-TATA- 1c, 5¢-GTGGCCCACTATTTACCCTCCCCTC-3¢; ONNFjBmut- 2c, 5¢-GCCTTCTCCCGAACCC-3¢; ON-NFjBmut-2m, 5¢-GGGTTCGGGAGAAGGC-3¢;...

Ngày tải lên: 30/03/2014, 20:20

... 5'-TGCCATGGCAATGGCTGCTAGGCTGTACTGCC-3' 5'-AACTGCAGTTAGGCAGAGGTGAAAAAGTT-3' The PCR product and the binding < /b> domain vector, pGBKT7 of 7.3 kb (MATCHMAKER Two-Hybrid System 3, BD Biosciences) were digested ... MATCHMAKER Library Construction and Screening Kit (Clontech Laboratories, Inc) First strand cDNA synthesis was carried out using µl of a random CDS III/6 Primer: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-NNNNNN3', ... assay screening indicate negative interaction between bait and library Colonies remained white HBV infection to be traced via interactions with the HBX protein < /b> which had been implicated in HBV-related...

Ngày tải lên: 20/06/2014, 04:20

... anti-A2M antibody (AbD serotec), chicken anti-ApoAI antibody (CHEMICON) and rabbit anti-VDBP antibody (AbD serotec) for two hour at room temperature After washing three times in PBS buffer (10 ... properly cited This is an Open Access from: http://www.jbiomedsci.com/content/17/1/58 Journal of et available article distributed under article is al; licensee BioMed Central References Marcellin ... Bulsara M, Rossi E, DeBoer B, Speers D, George J, Kench J, Farrell G, McCaughan GW, Jeffrey GP: Hepascore: an accurate validated predictor of liver fibrosis in chronic hepatitis C infection Clin...

Ngày tải lên: 10/08/2014, 05:21

... lipopolysaccharide-bindingand neutrophilinfected abilities Receiver-operating characteristic (ROC)markers all C- reactive protein < /b> between noninfected blood cell (wbc)infected discriminatingprocalciROC0.05)(il6), ... 0.01) Receiver-operating characteristic (ROC) curves comparing procalcitonin (pct), lipopolysaccharide -binding < /b> protein < /b> (lbp), C- reactive protein < /b> (crp), IL-6 (il6), white blood cell (wbc) and neutrophil ... systemic inflammatory response syndrome and sepsis(P 0.01) Receiver-operating characteristic (ROC) curves comparing procalcitonin (pct), lipopolysaccharide -binding < /b> protein < /b> (lbp), C- reactive protein...

Ngày tải lên: 12/08/2014, 23:23

Ngày tải lên: 15/08/2012, 11:00

Ngày tải lên: 15/08/2012, 11:01

Ngày tải lên: 15/08/2012, 11:01