cautionary tales the coming stem cell decade

... (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells would also be the targets of various immunotherapy ... ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population ... SKOV3 cells (Figure 3) We further isolated these colonies for the stem/ progenitor marker analysis Expression of cancer stem cell and self-renewal markers in circular colonies Cancer stem cells...

Ngày tải lên: 20/06/2014, 07:20

... interests in the Persian Gulf region in the coming decade In particular, the report explores the conventional military strength of Iran and Iraq, the potential for subversion, and the social and ... are the subject of current RAND research The Persian Gulf in the Coming Decade: Trends, Threats, and Opportunities limited budgets Even without the formidable U.S presence in the region, the ... other in the 1980s, leading to disruptions in the flow of oil and destabilizing the region Relations in the 1990s remained tense— both sides supported terrorists in the other’s country and otherwise...

Ngày tải lên: 06/03/2014, 17:20

... 1.2  Emerging stem cell therapies and the commercialization of biotechnology On the one hand, the stem cell sciences are clearly part of the biotech industry Most obviously, stem cells are biological ... viable products There are a number of similarities in how stem cell science and biotechnology progress On the other hand though, the unique properties of stem cells and the way stem cell products ... influencing the future shape of the stem cell market 1.4.2 The relationship between risk and return The other major factor limiting the potential success of emerging stem cell companies is the relationship...

Ngày tải lên: 20/03/2014, 06:20

... interests in the Persian Gulf region in the coming decade In particular, the report explores the conventional military strength of Iran and Iraq, the potential for subversion, and the social and ... are the subject of current RAND research The Persian Gulf in the Coming Decade: Trends, Threats, and Opportunities limited budgets Even without the formidable U.S presence in the region, the ... other in the 1980s, leading to disruptions in the flow of oil and destabilizing the region Relations in the 1990s remained tense— both sides supported terrorists in the other’s country and otherwise...

Ngày tải lên: 29/03/2014, 15:20

... differentiate embryonic stem cells from adult stem cells and point out that many tissues undergo continual replacement from stem cells They note the tremendous therapeutic potential of stem cells in replacing ... structure known as the stem cell niche At least in the hematopoietic (blood cell forming) system, the niche develops independently and the stem cells migrate to and colonize the niche (Schofield ... adult stem cells, although they are sometimes designated more conservatively as progenitor cells They are referred to as adult stem cells to distinguish them from embryonic stem cells, even if they...

Ngày tải lên: 11/06/2014, 12:52

... of the class I MHC, mediated by NSC-derived neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the ... parenchyma near the third ventricle (Figure 4) Migration of the BrdU+ cells along the corpus callosum and the ventricular wall was noted Small clusters of BrdU + cells and BrdU + cells with glial ... for 15 s Quantification of the gene of interest was accomplished by measuring the threshold cycles and comparing them to the standard curve to determine the copy number The process of calculating...

Ngày tải lên: 18/06/2014, 16:20

... accompanied by cell sorting to minimise the number of pluri/ multipotent stem cells in the cell preparation [86], or to separate tumourigenic stem cells from non-tumourigenic stem cells (by e.g cell sorting ... noted earlier the local environment in which the stem cell resides in the recipient may influence the biological properties of the stem cells, however only little is known whether these effects ... [81-85] Most likely the observed effect depends on the nature of the cancer cells, the Page of 14 characteristics of the used MSC, on the integrity of the immune system and on the timing and site...

Ngày tải lên: 18/06/2014, 19:20

... of cells called “cancer stem cells (CSCs)” that are responsible for forming the bulk of the tumor [14-16] They are similar to stem cells and may arise from the transformation of stem cells or the ... stem cell nature [13] Cells of Origin for Osteosarcoma Cancer Stem Cells CSCs have been proposed to arise either from the transformation of normal stem cells to cancerous stem cells or from the ... and therefore, there is a great need for developing new osteosarcoma treatments The Cancer Stem Cell Hypothesis The cancer stem cell hypothesis proposes that within a heterogeneous tumor there...

Ngày tải lên: 20/06/2014, 04:20

... of cells called “cancer stem cells (CSCs)” that are responsible for forming the bulk of the tumor [14-16] They are similar to stem cells and may arise from the transformation of stem cells or the ... stem cell nature [13] Cells of Origin for Osteosarcoma Cancer Stem Cells CSCs have been proposed to arise either from the transformation of normal stem cells to cancerous stem cells or from the ... and therefore, there is a great need for developing new osteosarcoma treatments The Cancer Stem Cell Hypothesis The cancer stem cell hypothesis proposes that within a heterogeneous tumor there...

Ngày tải lên: 20/06/2014, 07:20

... definition is the ability to generate cells of other types, but there are two problems with this First, there are clearly cells in the body that generate cell types other than themselves but ... probability that stem and progenitor cell progeny remain at the stem or progenitor cell stage (that is, they increase the probability of differentiation) Thus, the machinery for achieving stem cell- driven ... of control define a unique class of cells? The fact that so many characteristic behaviors of stem cells and stem cell systems - homeostasis in the face of the production of differentiated progeny;...

Ngày tải lên: 06/08/2014, 19:21

... 1b), as they stained the well-described putative colon mucosa stem cells, located at the basis of the crypts, or the transit amplifying zone, which are regarded to resemble the stem cell niche ... According to our hypothesis, that the intestinal stem cell marker LgR5 might also be suited to identify cancer stem cells, these might be the actively cycling Barrett (cancer) stem cells Our findings ... cancer stem cell compartment [8] The existence of cancer stem cells would explain why only a small minority of cancer cells is capable of extensive proliferation within the tumor Furthermore, these...

Ngày tải lên: 10/08/2014, 10:21

... tumor cells consists of three major steps: the receptormediated adhesion of tumor cells to the extracellular matrix, the degradation of the extracellular matrix by the proteinase secreted by the ... phenotypically defined endothelial cells at the single -cell level, suggesting these cells have the properties of hemangioblasts Page of 10 The main purpose of our article was to examine the immune characteristics ... secreted by the tumor cells, and the transfer and proliferation of tumor cells[36] So, the loose of ECM and secreted cytokines are important for the metastasis of the tumor cells from the primary tumor[37]...

Ngày tải lên: 10/08/2014, 10:21

... Further more, these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated by expression of GR-1, CD4/8, B220 and Sca-1 in BM cells ... LSK cells and three mice received Sall4B treated LSK cells (a) The population of donor cells (Ly5.2+) in the peripheral blood of the recipient mice was examined by flow cytometry assays with the ... compared with the Sall4 activities in the human system [19] We have hypothesized that the Sall4 gene is also involved in mediating cell fate decisions during hematopoiesis, helping to regulate the exquisite...

Ngày tải lên: 10/08/2014, 21:23

... prior to stem cell transplant and are listed in Table The individual iron parameter most closely associated with overall survival was the transfusion total, defined as the number of red cell units ... after transplant We further examined the cause of death among the 20 patients that died as a result of treatment related complications The majority of these patients (55%) had either http://www.jhoonline.org/content/2/1/44 ... setting Further evidence of the potential power of the iron score was seen in multivariate analysis, where the iron score maintained significance while controlling for other risk factors Using the Transplant...

Ngày tải lên: 10/08/2014, 22:20

... opportunities for stem cell therapy However, number of stem cells engrafted and differentiated after transplantation limit the treatment strategies Furthermore, ambiguity continues over the contribution ... bone marrow stem cells actually differentiate into hepatocytes and restore the liver functions [10] Moreover, the mechanism by which stem cells regenerate the respective parenchymal cells, heading ... parenchymal cells, heading to repair of the organs in vivo is yet to be completely understood Cell fusion, in which stem cells fuse with the somatic cell in the niche, had been suggested by many...

Ngày tải lên: 11/08/2014, 00:23

... mesenchymal, endothelial and other progenitor cells as well as mononuclear cells The cells were suspended into an opportune quantity of PBS-EDTA buffer containing 5% of human albumin The cell fraction ... we offered the opportunity to our patient of injection of autologous bone marrow stem cells into the osteonecrosis site lesion Methods In september 2008 the patient (who is the mother of one ... of the cell suspension were performed, including absolute CD34 and CD45 positive cell count and five colour MoAb panel for the identification of the cellular subpopulation (Table 1) Before the...

Ngày tải lên: 11/08/2014, 20:21

... hematopoietic stem cell transplantation The patients were treated at the Institute of Hematology and Blood Transfusion in Prague, Czech Republic The study was approved by the institutional review board The ... in the transplantation and contributed to the study design AK and TZ participated in the study design and helped to draft the manuscript TD intellectually contributed to the preparation of the ... value in the diagnosis of infection during the administration of ATG Competing interests The authors declare that they have no competing interests Authors' contributions HB carried out the laboratory...

Ngày tải lên: 13/08/2014, 16:20

... overall assessment of the importance of histone modifications on stem cell biology To evaluate the importance of histone acetylation on ES cell differentiation, we treated cells with the histone deacetylase ... order to examine the competence of the 'ES re' cells to contribute to the three germ layers, we placed them in hanging drops and observed that they formed EBs of normal morphology We then checked ... still capable of acquiring either one of the three cell fates To gain insight into the molecular mechanisms whereby TSA triggers stem cell differentiation, we analyzed the dynamics of histone H3...

Ngày tải lên: 14/08/2014, 08:21

... Embryonic stem (ES) cells are derived from the inner cell mass of the embryo and possess the property of pluripotency, which is the ability to develop into any cell lineage of the organism [1-3] The ... 19 arrays (e, f) The chromosomal position of the genes, as well as the genomic scale, is represented along the xaxis The fold enrichment of the probes is shown on the y-axis These enrichment ... recovery distance from the edge of the other, then they were considered 'matched' The typical recovery distance used was kb, although this was varied systematically for the recovery curves A recovery...

Ngày tải lên: 14/08/2014, 20:22