cathodoluminescence energy dispersive x ray analysis and infrared spectroscopy studies
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
Ngày tải lên :
16/02/2014, 09:20
... backbone and side chain 13Ca and 13C¢ and 13Cb carbons are assigned to the extent of 97%, 93% and 93%, respectively (BMRB accession number 6940) [24] The H–15N correlation in the TROSY experiment ... form of nPS using X- ray crystallography, as described below A Solution and refinement of the structure using X- ray crystallography B The crystal structure of the pantoate–nPS complex was solved by ... density maps and facilitate further rebuilding and improvement of the molecular model, until no unexplained electron density remained, and the Rcryst and Rfree values converged at 18.9% and 24.4%,...
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Tài liệu Báo cáo khoa học: Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc
Ngày tải lên :
18/02/2014, 17:20
... when the complex is formed The ligands cellobiose, cellotetraose and cellohexaose were studied Results and Discussion The crystal structure of CtCBM11, the binding cleft and its ligand specificity ... in the X- ray structures of CBM4 and CBM17 complexed with cellopentaose and cellohexaose, respectively [13,23] The involvement of the tyrosine residues in the stabilization of the complex cannot ... two six-stranded anti-parallel b-sheets that form a convex side (b-strands 1, 3, 4, 6, and 12) and a concave side (b-strands 2, 5, 7, 8, 10 and 11) The concave side is decorated by the side chains...
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Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
Ngày tải lên :
07/03/2014, 17:20
... structural analysis of submillisecond folding events in cytochrome C Acc Chem Res 31, 717–725 2453 X- ray and unfolding studies of cyt c-550 31 Yeh SR & Rousseau DL (1999) Ligand exchange during ... by way of peroxidase activity assays and NMR spectroscopy Results Structure determination and overall structure of the various ferricyt c-550 models For the M100K variant two X- ray datasets were ... M100K(rt) and the wt-model, a noticeable difference FEBS Journal 272 (2005) 2441–2455 ª 2005 FEBS J A R Worrall et al X- ray and unfolding studies of cyt c-550 Table Data processing and refinement...
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Báo cáo Y học: Determinants of the inhibition of a Taiwan habu venom metalloproteinase by its endogenous inhibitors revealed by X-ray crystallography and synthetic inhibitor analogues pdf
Ngày tải lên :
08/03/2014, 23:20
... superfamily of metzincin which exhibits some typical structural features, such as the Met-turn and active-site consensus HExxHxxGxxH sequence [15–17] Some organisms and mammalian tissues recently ... Huber, R & Bode, W (1995) X- ray structures of human neutrophil collagenase complexed with peptide hydroxamate and peptide thiol inhibitors Implications for substrate binding and rational drug design ... off by a solvent mixture of trifluoroacetic acid and ethanedithiol, and solvent was evaporated to dryness The resins were then washed with cold ether and the peptides were extracted with 5% acetic...
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Báo cáo Y học: Boar salivary lipocalin Three-dimensional X-ray structure and androstenol/androstenone docking simulations pot
Ngày tải lên :
17/03/2014, 17:20
... Docking experiment with androstenol and androstenone The previously identified natural ligands androstenol and androstenone have been built with the program ACD/CHEMSKETCH [18] The parameter and topology ... the model of the complex of androstenol bound in SAL, with in (A) and (C) the ligand in position ÔinÕ, with the OH pointing inside the cavity and, in (B) and (D) the ligand in position ÔoutÕ, ... positions of androstenone (not shown) are undistinguishable from those of androstenol In (A) and (B): view of the lipocalin scaffold and the bound ligand In (A) and (B) the rings of the ligand are...
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Báo cáo khoa học: X-ray crystallography and structural stability of digestive lysozyme from cow stomach doc
Ngày tải lên :
23/03/2014, 04:21
... residues Glu35 and Asp52 (numbering for HEWL), for BSL2 and other lysozymes, with propka 2.0 [16] The predicted pKa values were 6.15 and 4.27 for BSL2, 5.93 and 4.20 for HEWL, and 4.89 and 3.84 for ... acetate (for pH and 5) and sodium phosphate (for pH and 7) buffer The ionic strength of each buffer was adjusted to 0.1 [40] Lysozyme solution and M lysodeikticus suspension were mixed, and the decrease ... Gibbs free energy with denaturant and that without denaturant respectively, and R, T, K, m and C are the gas constant, absolute temperature, equilibrium constant, cooperativity index, and denaturant...
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Báo cáo khoa học: Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf
Ngày tải lên :
23/03/2014, 07:20
... LiOH provided the expected products PAH and FPAH as lithium salts in 28% and 15% overall yield, respectively X- ray crystallography Recombinant T brucei enolase was expressed and purified as previously ... 20 mm of ligand (PEP, PAH or FPAH) for and flash-cooled X- ray diffraction data were collected from two native crystals (referred to as sulfate_2 and sulfate_3 in Table 1) and four ligand-cocrystallised ... sulfate_2, PEP and PAH_1 structures is shown in Fig Unexpected variability in inhibitor complex structures Comparison of the substrate and inhibitor complexes shows that the His156-out and His156-in...
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Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis pot
Ngày tải lên :
30/03/2014, 02:20
... central six-strand mixed b sheet (b2, b1, b3, b5, b6 and b4; b5 is antiparallel to the others) flanked by three a-helices (a1, a9 and a8) on the inner side and by two a-helices (a2 and a3) and two ... 1P74, 1WXD, 2EGG, 2HK7, 2HK8 and 2NLO), (b) binary complex bound with either cofactor (1NPD, 1NVT, 1NYT, 1O9B, 1P77, 1VI2 and 2CY0) or substrate (2D5C, 2GPT and 2O7Q) and (c) inactive (2HK9_A and ... amides of Asn58 and Asn85 The C4-hydroxyl group of shikimate interacts with the carboxylate group of Asp100 and with the side-chain amides of Asn85 and Lys64 The C3-hydroxyl group forms extensive hydrogen...
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Báo cáo lâm nghiệp: "Relationships between the intra-ring wood density assessed by X-ray densitometry and optical anatomical measurements in conifers. Consequences for the cell wall apparent density determination" pps
Ngày tải lên :
08/08/2014, 01:22
... cell and lumen models Let X = · x = width of the double cell wall: s = R · T – [(R – X) · (T – X) ], s = X · (T + R) – X2 , s / S = cell wall proportion If drx = the wood density measured by x ray: ... apparent density of the cell wall = drx / cell wall proportion = drx · S/s = drx · R · T / [X · (T + R) – X2 ] = drx · R · T / [X · (T + R – X) ] 2.3.2 Hexagonal model ST = area of measurement ... H2 And again, if drx = the wood density measured by x ray: apparent density of the cell wall = drx / cell wall proportion = drx · S/s = drx · H2 / (H2 – H’2) The use of rectangular and hexagonal...
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Báo cáo khoa học: "Predicting oak wood properties using X-ray inspection: representation, homogenisation and localisation. Part I: Digital X-ray imaging and representation by finite elements" ppsx
Ngày tải lên :
08/08/2014, 14:21
... microfocus X- ray source incident X- ray beam thin sample residual X- ray beam scintillator visible light prism cooled CCD detector lead shield Figure Schematic diagram and overview of an X- ray device ... 1% of the incident Xray beam intensity The quality of the image (contrast between lumen and cell wall) involves a great number of X- photons to be detected The experimental X- ray conditions are: ... “Background” (IB), without X- ray illumination, allows offset and average noise level be to evaluated and subtracted A second image is obtained without sample but with X- ray illumination It corresponds...
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Báo cáo sinh học: "Developmental time at which spontaneous, X-ray-induced and EMS-induced recessive lethal mutations become effective in Drosophila melanogaster" potx
Ngày tải lên :
14/08/2014, 20:20
... lethals were extracted in the same manner as for wild lethals For the phase determination experiment, 30 2nd chromosomes and 30 3rd chromosome X- ray induced lethals and 20 2nd chromosome and 30 3rd ... On the other hand, other differences between X- ray- induced, EMS-induced and natural lethal mutations evidently exist As can be seen from table III, a very high proportion of X- ray- induced lethal ... I) were randomly distributed among the types of lethals The x2 values were highly significant between natural and both types of induced lethals, but not significant between X- ray- induced and EMS-induced...
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Báo cáo y học: "Dual energy x-ray absorptiometry analysis contributes to the prediction of hip osteoarthritis progression" pdf
Ngày tải lên :
09/08/2014, 14:22
... study only participants with bilateral radiographs and dual energy X- ray absorptiometry (DXA scans) of adequate quality measured at baseline and after two years follow up were included in the ... (ΔK-L) and the difference between the most affected vs contralateral DXA-based parameters at baseline (ΔDXA) All models were adjusted for age, weight, height, and sex To reduce the number of DXA ... between progressors and non-progressors were found in the regions close to the joint space (superior and medial part of the head and the outer arcs and 4, Table and Figure 1) As expected, these values...
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Báo cáo y học: "Application of Small Angle X-ray Scattering (SAXS) for Differentiation between Normal and Cancerous Breast Tissue"
Ngày tải lên :
02/11/2012, 11:08
... AP and Dance DR Breast cancer diagnosis using scattered X- rays J Synchrotron Rad 2000; 7: 348-352 Malden CH, Speller RD, et al A CdZnTe array for the detection of explosives in baggage by energy ... lobular carcinoma and in-situ ductal carcinoma studied in this work is referred to as glandular tissue Therefore, it is expected different peak positions will exist for carcinoma and fibrocystic ... temperature Histological analysis of the samples gave the following breakdown of tissue types: 20 pure adipose tissues, 17 mixed (adipose plus fibroglandular) normal tissues, 41 carcinoma and 21 fibrocystic...
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Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx
Ngày tải lên :
14/02/2014, 03:20
... scanning calorimetry (DSC); X- ray powder diffraction (XRPD); combined, simultaneous, and dynamic differential scanning calorimetry /X- ray powder diffraction (DSC/XRPD); and high performance liquid ... DSC and XRPD experiments Additional studies would be required to further understand the implications that sample 42 places on the assignment and DSC characterization of Form III Forms IV and ... approximately 245 °C and a peak at approximately 256 °C XRPD data from DSC/XRPD analysis are shown in Figure 18 This provides evidence of the solid–solid phase transition and the existence of a hydrous...
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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf
Ngày tải lên :
18/02/2014, 13:20
... residues 29, T34 and E131 are shown The hydrogen bonds between N29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein are shown as green broken lines, and the ion ... the protein, and a and b represent the a helix and b strand, respectively The side-chains of the mutated and parent amino acid residues at the six mutation sites are shown D39 ⁄ G39 and M76 ⁄ V76 ... 6·-RNase HI and wild-type proteins (full line) and between molecules C and D (broken line) a Helices and b strands are indicated by bars helices, and around Gly128 in a loop between the aV helix and...
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Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot
Ngày tải lên :
22/02/2014, 07:20
... fitting and analysis For a mixture of monomeric and dimeric scattering species, the radius of gyration is given by: R2 ¼ fM R2 þ fD R2 g gM gD ð7Þ where fM and fD are the mass fractions of monomer and ... a model and the experimental data: N X IðSj Þ À Iexp ðSj Þ 2 v2 ¼ ð8Þ N À j¼1 rðSj Þ where for each momentum transfer value, Sj, I(Sj) is the theoretical scattering, Iexp(Sj) is the experimentally ... solution X- ray scattering These pieces were modelled into the AA and BD dimers as extended polypeptide [26], and produced an improvement in the fit of the AA model at S values between 0.15 and 0.2...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
Ngày tải lên :
07/03/2014, 17:20
... decreased in the series b-d-xylohexaose (Xyl6) > b-d-xylopentaose (Xyl5) > b-d-xylotetraose (Xyl4) > b-d-xylotriose (Xyl3), with no detectable afnity for b-d-xylobiose (Xyl2), which is consistent ... (Da)a TRX I (none) None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me None S-Xyl2-Me S-Xyl3-Me S-Xyl4-Me S-Xyl5-Me 19046.920 n.b 19508.080 19656.081 19804.091 ... FEBS J Janis et al ă Thioxylo-oligosaccharide binding to xylanases Fig MS titration curves for TRX I (5 lM), TRX II (10 lM) and CTX (10 lM) with S-Xyl5-Me, S-Xyl4-Me and S-Xyl3-Me (initial concentrations...
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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
Ngày tải lên :
08/03/2014, 10:20
... is known from X- ray structural analysis of wild-type cyt b5 [21] that the core consists of b-strand III (Tyr27–Leu32), b-strand II (Thr21–Leu25), b-strand I (Lys5–Tyr7) and a-helix I (Thr8– His15) ... Refinement and structural ˚ analysis of bovine cytochrome b5 at 1.5 A resolution Acta Crystallogr D52, 65–76 Xue, L.L Wang, Y.H Xie, Y Yao, P Wang, W.H Qian, W Huang, Z .X Wu, J & Xia Z .X (1999) ... mutant has been determined by X- ray analysis Based on the molecular structure and the above detailed studies the essence of these unusual behaviors is discussed MATERIALS AND METHODS Protein preparation...
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Báo cáo khoa học: "What to do when lexicalization fails: parsing German with suffix analysis and smoothing" doc
Ngày tải lên :
17/03/2014, 05:20
... trigram x1 x2 x3 with c x1 x2 x3 c x i xi xi if c xi xi c x i xi d3 if c xi xi £ Figure 1: Smoothing estimation based on the Brants (2000) approach for POS tagging for all possible contexts As ... 1 1 d1 if d3 max d1 d2 d3 then λ3 λ3 c x i xi xi elseif d2 max d1 d2 d3 then λ2 λ2 c x i xi xi else λ1 λ1 c x i xi xi λ1 P B i A B i 1 if c xi if c xi c xi N £ Bi £ We experiment with three ... they are simple to estimate and parse, and adding smoothing makes both estimation and parsing nearly as complex as with fully lexicalized models However, because lexicalization adds little to...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
Ngày tải lên :
30/03/2014, 20:20
... 164 x, y,z x +1,y,z x +1,y,z 1 -x, y +1 ⁄ 2,-z x, y,z x, y,z x, y,z x, y,z x- 1,y,z 1 -x, y-1 ⁄ 2,1-z x, y,z x, y +1,z x, y +1,z x, y-1,z x, y-1,z x, y,z 1 -x, y-1 ⁄ 2,1-z x, y,z 1 -x, y +1 ⁄ 2,-z x, y +1,z x, y,z x, y-1,z ... x, y-1,z x, y,z x, y-1,z 3.024(4) 2.783(5) 3.016(4) 3.138(5) 3.021(5) 2.776(5) 3.273(5) 3.122(5) 2.814(4) 2.848(5) 3.221(4) 2.767(5) 3.083(5) x, y,z x, y,z 1 -x, y-1 ⁄ 2,-z 1 -x, y-1 ⁄ 2,-z x, y,z x, y,z x, y,z ... form (in dimethyl sulfoxide and TFE) and monocationic form (in water and DPC micelles) for EM2, and zwitterionic form (in dimethyl sulfoxide, TFE, water, DPC micelles) and monocationic form (in...
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