case study intelligent platform management interface driver

... comparable to those with radioisotopes Moreover, these techniques can be used in cases when the chemical elements under study not possess any radioactive isotope with a half-life suited to tracer ... number of investigations have thus been devoted by many authors to study the kinetics of enzyme-catalysed reactions: in the simplest case (enzymes of the “Michaelis–Menten” [M–M] type studied in diluted ... parameters, Vm and Km; in more complicated case (e.g allosteric 14 M Thellier enzymes) the curve may become sigmoid, etc Here, we have studied a few cases in which an enzyme was studied under...

Ngày tải lên: 29/05/2014, 23:58

... been used as the model organism for the study of G-protein machinery [30–32] Much knowledge of G-protein signaling in yeast has been accumulated and used to study cellular processes, including protein ... a model organism to study transport and homeostasis of alkali metal cations Physiol Res 53, S91–S98 Lushchak VI (2006) Budding yeast Saccharomyces cerevisiae as a model to study oxidative modification ... – protein–protein interactions Yeast–mammal chimeric Ga system Medici et al [78] constructed an intelligent system for analysis of protein–protein interactions by managing heterotrimeric G-protein...

Ngày tải lên: 06/03/2014, 11:20

... hypervariable sequences and lengths in the six-loop CDR (Fig 1A) that bind to the antigen The first study of grafting into the CDR replaced the CDR loops in a human antibody with those from a mouse ... to somatostatin receptor 5, into the CDR and CDR loops in the light chain (CDR L1 and CDR L2) to study the potential of Fv as a scaffold protein for grafting They investigated deviations in the ... generating binding proteins, Fvs of antibodies are most commonly used as scaffold proteins In the case of single-chain Fvs and Fabs, artificial libraries of CDR loops have been constructed from synthetic...

Ngày tải lên: 06/03/2014, 11:20

... For example, the Smith and Eyre-Walker study [54] analyzed only 392 genes in the disease set and 2,038 genes in the non-disease set, and the Huang et al study [55] included 1,178 human disease ... methods and analyzed SNP A/S ratios for different classes of proteins and residues in an attempt to study the natural selection of these properties from the SNP perspective Our comprehensive analysis ... ratio is 0.28 The values of A and S are both two times more than what have been reported in a small study [11], but the A/S ratio is similar SNP A/S ratio as a measure for selective constraints To...

Ngày tải lên: 14/08/2014, 08:21

... Materials 83 2.3.1.1 Antibodies used in this study 83 2.3.2 Methods 83 2.3.2.1 Protein concentration determination 83 2.3.2.2 Immunoprecipitation study 85 2.3.2.3 Cell surface biotinylation 86 ... transcription reaction (20 µl) 66 2.2.1 Molecular and microbial stimuli used in this study 75 2.3.1 Antibodies used in this study 84 2.4 Primers used in the cloning of IL-4, IL-4Rα, γC and CD14 cDNA 91 ... Adopted from Xu et al., (2000) 18 Three interactive interfaces in the TIR domains for TLR signaling has been proposed by (Xu et al., 2000): i) The first interface (R face) mediates oligomerization between...

Ngày tải lên: 12/09/2015, 08:20

... are too short for the formation of these interactions, or due to disorder in helix H69, as is the case in the crystal structure of the large ribosomal subunit from H marismortui, H50S [2,3] None ... with H69, the other likely to be a conformational switch, assisting translocation near the subunit interface [7,8] We found that the space available for A2602 throughout the rotatory motion of the ... somewhat released when the participation of the phosphate, rather than the base is required, as is the case of G2494, a rear-wall moiety that is less conserved Ó FEBS 2003 Ribosomal catalytic and regulatory...

Ngày tải lên: 23/03/2014, 17:22

... site (see Fig S3 and Doc S1) Thus, the propensity of hydrophobic residues on the putative binding interface of the MSR FMN domain suggests less emphasis on electrostatic interactions mediating hMS–MSR ... anaerobic buffer (10 mm potassium phosphate, pH 7.2) The enzymes were reduced to the 1, and (in the case of full-length MSR) reduced states by titration with dithionite The UV–visible spectra of the ... measured by the AqCbl ⁄ dithiothreitol radioactive assay described above Acknowledgements This study was funded by the UK Biotechnology and Biological Sciences Research Council N.S Scrutton is...

Ngày tải lên: 18/02/2014, 08:20

... transcript level, we used primary cultures of human fibroblasts These cells are a good model to study inflammatory processes, because, in contrast to HepG2 cells, expression of IL-1b in fibroblasts ... for MCPIP cDNA (control of overexpression, right panel) or for IL-1b cDNA (left panel) In each case, the IL-1b transcript level after LPS treatment was expressed as a percentage of the basal ... MCPIP mRNA level for both forms (Fig 5A, lanes and 6, upper bands) Moreover, in a co-transfection study with the IL-1b-expressing vector, the level of mRNA for the mutant form of MCPIP was higher...

Ngày tải lên: 18/02/2014, 14:20

... those states and the different conformational stability between the HPr species [32] Thus, the study of the stability among the different HPr members will allow one to establish whether there ... of the protein with cold-denaturation detectable at temperatures above 273 K The possibility to study both cold- and heat-denaturation over a range of urea concentrations has made possible the ... spectra were collected on a SLM 8000 spectrouorometer (Spectronics Instruments, Urbana, IL, USA), interfaced with a Haake water bath A 0.5-cm pathlength quartz cell (Hellma) was used Urea-unfolding...

Ngày tải lên: 19/02/2014, 12:20

... 1.0 (18 2) c 37 0.02 (0.21 0.08) 4.4 350 600 17 16 c a Metal ion concentration was mM in all cases b From [6], calculated from the Trp uorescence quenching in the unlabeled enzyme c This work, ... experiments were carried in the presence of several combinations of substrates and metal ions In all cases the decay of the uorescence intensity of the P-pyridoxyl group ts quite well to a biexponential ... P-pyridoxyl group, which is in agreement with the acrylamide quenching experiments In conclusion, this study shows that the pyridoxyl chromophore of PLP is an ideal probe to detect environmental changes...

Ngày tải lên: 21/02/2014, 01:21

... residues in a fixed order, e.g S ⁄ T-X-X-S ⁄ T for casein kinase I [25–27] When dephosphorylation proceeds in the reverse order, we will refer to this case as a strictly sequential mechanism (Fig 1, ... interactions for a systematic simplification of the model [20] This allows us to obtain, in special cases, explicit solutions for the steady-state response curves and phosphorylation times, and to ... cycles can potentiate differential control of the phosphorylation sites by the same kinase This study provides a link between the structural, kinetic and thermodynamic aspects of complex multisite...

Ngày tải lên: 07/03/2014, 10:20

... CSQ overlay technique to study complex formation in developing, transforming and ageing skeletal muscle fibers Blot overlays are a technically challenging approach to studying complex protein–protein ... using the optimized CSQ-POD overlay procedure presented in this study are an excellent example of applying a direct decoration method to studying heterogeneous membrane assemblies under differing ... I-A band interface, and the process is completed by the transverse orientation of dihydropyridine receptor-containing membrane domains [45] The CSQ-POD overlay analysis presented in this study indicates...

Ngày tải lên: 08/03/2014, 10:20

... splice variant has been detected in all melanoma and most of lymphoma cell lines tested 1598 In this study, we focus on the biochemical properties of both splice variants of hGBP5, hGBP5a ⁄ b (amino ... binding, and, especially when using the GTP analogs, to the formation of higher-order oligomers In the case of hGBP1, the entropy changes for nucleotide binding are generally positive but less than kcalÆmol)1 ... contributions of nucleotide binding and the coupled oligomerization show opposing signs In the case of hGBP1, release of water from the nucleotide binding pocket and the protein surface may counterbalance...

Ngày tải lên: 15/03/2014, 10:20

... residues to either alanine (Ala) or glutamic acid (Glu), as shown in Fig 1B, left panel; in many cases Glu can functionally substitute for phospho-Ser/Thr residues Mutation of the first set of Ser ... position of functional domains is indicated, and regions fused to GST protein and used in this study are mapped below (B) Extracts from control [C] or virus-infected [V] HEC-1B cells labeled ... truncations from the C-terminus led to the detection of slower migrating forms (Fig 5C, top panel) In the case of virus-activated IRF-3 (Fig 3B), the slower migration on deoxycholate-PAGE could be due to...

Ngày tải lên: 17/03/2014, 10:20

... considered as a protein with an exclusive location in the endoplasmic reticulum [33], but in that study it was demonstrated that it also exists in the cytosol where it impairs cellular functions ... expressing HIP/PAP Two independent methods were used to address this question: assay of PKA activity and study of the expression of a gene whose promoter contains a sequence responding to PKA These approaches ... L-29, a soluble lactose-binding lectin, is phosphorylated on serine and serine 12 in vivo and by casein kinase I J Biol Chem 268, 26712–26718 36 Cowles, E.A., Agrwal, N., Anderson, R.L & Wang,...

Ngày tải lên: 23/03/2014, 13:20

... Biochem 270) 3093 introducing a possible confounding factor not present with chicken TnI In this study we have labeled the two Nterminal cysteine residues of chicken pectoral muscle TnI with either ... sequences of skeletal and cardiac TnC and TnI are different [19,23–26], in particular, at their binding interfaces These differences might alter the TnI–TnC interaction and thus affect muscle regulation ... substitutions at Ca2+ binding loop I [23], porcine cardiac TnC binds mol Ca2+ per mol protein [26] In this study, we also compared the effects of sTnC and cTnC to test if an isoform-specific interaction of...

Ngày tải lên: 23/03/2014, 21:20

... is characterized by a prevalence of hydrophobic residues in the case of the subunits B and C and of hydrophilic residues in the case of the subunit A Val20, Trp79, Leu82, Ile84, Pro113 and Tyr114 ... ability to interact with each other in terrestrial vertebrates remains to be clarified In the present study, we report on the structure of a complex formed by the association of human TTR, human holoRBP ... 2G4E) [39] TTR variants at neutral pH are very similar to that of the wild-type protein In both cases, the amino acid replacements are not associated with significant local conformational changes...

Ngày tải lên: 30/03/2014, 02:20

... Techniques, edited by Hans J Vogel, 2001 172 Calcium-Binding Protein Protocols, Volume 1: Reviews and Case Histories, edited by Hans J Vogel, 2001 171 Proteoglycan Protocols, edited by Renato V Iozzo, ... wrappedaround RNA polymerase II (POL II), allowing contacts by the Xeroderma Pigmentosum Group B (XPB) helicase of transcription factor TFIIH to the template strand of the melted DNA duplex immediately upstream ... providing the key to the genetic plan as well as mechanisms for its maintenance and evolution The study of these interactions is therefore fundamental to our understanding of growth, development,...

Ngày tải lên: 08/04/2014, 12:51

... equimo- EvdW conceived the study, carried out the experiments, and prepared the manuscript KEP conceived the study, helped construct chimaeric genomes and supervised the study DPM helped prepare ... genetic modules, which may range in size from a few nucleotides in the case of some regulatory sequences to many megabases in the case of an entire genome Here we present evidence that the mp-cp cassette ... extreme cases, were almost as wide as the leaf, and usually extended unbroken for several centimetres (Figure 1) In severe KNco infections, plants and leaves were noticeably stunted and in some cases...

Ngày tải lên: 20/06/2014, 01:20