cardiac remodeling and cell death in heart failure

... systolic time and heart rate and expressed in seconds per minute Diastolic time was calculated as the cardiac length (RR interval) minus the duration of systole To define diastolic duration during exercise ... heterogeneous termination LV isovolumic 338 time intervals were found to be increased at the expense of LV diastolic time in heart failure patients (17,18) Since our findings and other previous ... lack of increase in LV dyssynchrony during dobutamine stress in patients with wide QRS duration (20) In contrast, Kurita et al reported on an increased mechanical dyssynchrony during pacing–induced...

Ngày tải lên: 05/11/2012, 11:32

... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... fusion gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of ... better understanding of carcinogenesis in diverse tissues, e.g the mammary gland (Johnstone et al, 2002) Both extrinsic and intrinsic stimuli can initiate apoptotic signaling, which involves the...

Ngày tải lên: 23/03/2014, 17:20

... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... fusion gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of ... better understanding of carcinogenesis in diverse tissues, e.g the mammary gland (Johnstone et al, 2002) Both extrinsic and intrinsic stimuli can initiate apoptotic signaling, which involves the...

Ngày tải lên: 27/06/2014, 11:20

... England Journal of Medicine CORRECTION Sudden Death in Patients with Myocardial Infarction and Left Ventricular Dysfunction, Heart Failure, or Both Sudden Death in Patients with Myocardial Infarction ... LXRα is expressed predominantly in tissues involved in lipid homeostasis in the liver, intestine, adipose tissue, and macrophages LXRα and LXRβ are equally effective in promoting reverse cholesterol ... reviewed all deaths and episodes of cardiac arrest with resuscitation in a blinded fashion, using source documentation provided by the site investigators Deaths were classified as having cardiovascular...

Ngày tải lên: 27/06/2014, 00:20

... required, at least in part, for imatinib-induced apoptosis in BCR–ABL-transformed murine progenitor cells [64] and in BCR–ABL+ K562 CML cells in response to both imatinib and nilotinib [65] INNO-406, ... imatinib therapy have point mutations in the BCR–ABL kinase domain, including the T315I gatekeeper mutation that impairs imatinib binding [58,59] Accordingly, new therapies are being tested and ... [44–46] It is interesting to note that in all cases MEK inhibitor-induced BIM expression alone was not sufficient to induce a dramatic increase in cell death; pronounced increases in apoptosis...

Ngày tải lên: 16/03/2014, 00:20

... hand, in non-neuronal cells, cytoplasmic retention of ERK1 ⁄ is required for death- associated protein kinase-mediated cell death [35] Thus, ERK1 ⁄ might promote cell death depending upon the cell ... it remains to be seen whether ERK5 is also involved in ERK1 ⁄ 2-implicated cell death paradigms ERK1 ⁄ in cellular models of neuronal death The first evidence for a role of ERK1 ⁄ in cell death ... of multiple mitogen-activated protein kinases in an oligodendrocyte cell line: role of extracellular signal-regulated kinase in hydrogen peroxide-induced cell death J Neurochem 72, 112–119 12 Stanciu...

Ngày tải lên: 29/03/2014, 08:20

... ANT1-silencing induces glioblastoma cell death A Fig Analysis of ANT1 and ANT2 expression in nontransfected ADF cells and in ANT1- and ANT2-silenced ADF cells (A) Number of ANT1 and ANT2 transcripts in ... reduction in cell viability induced by ANT1 siRNA requires protein synthesis ANT1-silencing induces ADF cell apoptosis and a non-apoptotic cell death modality similar to paraptosis To gain more insight ... ANT1-silencing induces glioblastoma cell death A Lena et al Because of their multitask nature, ANTs are involved in several aspects of cell metabolism, as well as in cell survival ⁄ death processes, and...

Ngày tải lên: 29/03/2014, 09:20

... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining ... VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in vitro NB4-R2 cells were treated ... pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied the inhibition of Aurora kinases in NB4-R2 cells using VX-680 Aur-A activation was inhibited by VX-680 at different...

Ngày tải lên: 18/06/2014, 19:20

... matrix shown in C is indicated in D Similar analysis as in A and B was used, but included only MPTP-treated values and assigned value of for no treatment and 10, 30 and 50 for increasing dosage ... to dopaminergic neuronal death by oxidizing dopamine to a reactive dopamine quinone [51], by increasing DNA oxidation [7], or through increased microglial activation leading to chronic inflammation ... of oxidative and inflammatory insults The positive feed back loop may continue until the additional neuronal death (~50% of the remaining of the initial death) combined with the initial loss...

Ngày tải lên: 19/06/2014, 22:20

... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining ... VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in vitro NB4-R2 cells were treated ... pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied the inhibition of Aurora kinases in NB4-R2 cells using VX-680 Aur-A activation was inhibited by VX-680 at different...

Ngày tải lên: 20/06/2014, 03:20

... perifosine and the prototypic intravenously applicable ErPC3, in preclinical and clinical investigations is based on their ability to induce apoptosis in tumor cells and their ability to increase ... effect of combined treatment with ErPC3 and ionizing radiation PC3 cells (A), DU145 cells (B), and LNCaP cells (C) were treated with increasing concentrations of ErPC3 (1-100 µM) and ionizing radiation ... the ErPC3-induced down-regulation of Mcl-1 and p-Akt may be sufficient to overcome the cellular death threshold and to induce apoptotic death of LNCaP cells [16,36] In PC3-cells, ionizing radiation...

Ngày tải lên: 09/08/2014, 09:20

... effective interventions to prevent organ failure in sepsis During sepsis, there is extensive apoptotic death of lymphocytes and gastrointestinal epithelial cells [7] The increased apoptotic death ... these promising findings must be tempered by the new study showing that multiple pathways might be activated in dying cells during sepsis [1] The simple inhibition of a particular cell death route ... liver injury [9] In addition to apoptosis, certain caspases, such as caspase-1, are involved in cytokine maturation by proteolytically processing the inactive precursors of interleukin (IL)-1β and...

Ngày tải lên: 13/08/2014, 18:22

... cullin3 Cylindromatosis cytochrome c death- associated protein-1 death- associated protein kinase DEP domain containing mTOR-interacting protein direct inhibitor of apoptosis-binding protein with ... protects against EBSS-induced cell death in TSC2-/- cells 89 3.2.5 Nutrients supplementation protects against cell death in TSC2+/+cells, but enhances cell death in TSC2-/- cells under ... UVRAG-Bif1-Beclin 1-p150-PI3KC3 (Janku et al., 2011) In contrast, the RUN domain and cysteine-rich domain containing Beclin interacting protein (Rubicon) interacts with UVRAG–Beclin 1–hVps34–p150...

Ngày tải lên: 10/09/2015, 09:30

... direct linkage between JNK signaling and the apoptotic cell- death machinery, especially mitochondrial cell death, remains unclear In the present study, we investigated the basis for interaction ... understanding of cell- death events after ionizing irradiation and development of molecular targets for cancer treatment Results To examine the kinetics of the apoptotic cell death induced by ionizing ... in the regulation of apoptotic cell death in response to various stimuli To investigate a potential involvement of MAPK in ionizing radiationinduced cell death, we employed specific chemical inhibitors...

Ngày tải lên: 07/03/2014, 05:20

... rapidly and extensively induce cell death in the human breast cancer cell line L56Br-C1 This cell line carries a germ-line mutation (1806 C fi T) in the BRCA1 tumour Fig Propidium iodide-stained ... being involved in DENSPM-induced cell death in L56Br-C1 cells The Ó FEBS 2002 1038 C Hegardt et al (Eur J Biochem 269) pathway involves a change in mitochondrial transmembrane potential and in ... Correlations between polyamine analogue-induced increases in spermidine/spermine N1-acetyltransferase activity, polyamine pool depletion, and growth inhibition in human melanoma cell lines Cancer Res 51,...

Ngày tải lên: 17/03/2014, 23:20

... normal human PBLs and B-lymphoid cells, by inducing mitotic catastrophe and erosion of telomeric DNA selectively in the cancer cells and by inducing cell proliferation in the normal cells Although ... results further imply that Ruta induces death- signaling pathways in human glioma brain cancer cells, both in vivo and in vitro, and survival-signaling pathways in normal B and T lymphocytes Discussion ... cancer cells, by either inducing cell death or preventing further proliferation; b) to explore the molecular mechanism of cell death by Ruta + Ca3(PO4)2 treatment of brain cancer cells in vitro; and...

Ngày tải lên: 22/03/2014, 17:20

... phosphate buffer (pH 7.8) containing 1.5 mgÆmL)1 nitro-blue tetrazolium and a further 10 in the same buffer containing 28 mM TEMED and 28 lM riboflavine After a brief washing in water, the gel was revealed ... from control cells and GAL1/GAL10-driven Bax-expressing cells cis-Parinaric acid was incorporated in mitochondrial membranes, and fluorescence measurements were carried out as indicated in the methods ... dioctylphtalate on h-Bax-induced cell death kinetics WtB1 cells were grown in a commercial Yeast Nitrogen Base medium (Difco, h), or in a home-made minimal medium in the absence (j) or in the presence...

Ngày tải lên: 23/03/2014, 21:20

... FEBS S Cagnol and J.-C Chambard ERK and cell death Table Continued Stimuli inducing cell death Duration of ERK activation promoting cell death Human melanoma C8161,WM164 Mel Juso Betulinic acid 96 ... Daniels MA (2009) ERK and cell death: ERK location and T cell selection FEBS J Martin P & Pognonec P (2009) ERK and cell death: cadmium toxicity, sustained ERK activation and cell death FEBS J Taylor ... phospholipase A2 activity is induced by CD95 ligand cross-linking in the mouse derived Sertoli cell line TM4 and is required to trigger apoptosis in CD95 bearing cells Cell Death Differ 7, 916–924...

Ngày tải lên: 29/03/2014, 08:20

... signaling is important for determining cell fate decisions in a diverse number of organisms and cell types (reviewed in [7,8]) In thymocytes, c-JunNH2-terminal kinase (JNK) [9], p38 [10] and extracellular ... Teixeiro and M A Daniels induction of pERK that is localized to the plasma membrane (Fig 2) On the other hand, positive selecting ligands induce a slow and sustained activation of ERK originating ... LAT In line with this, positive and negative selecting ligands show quantitative differences in total phosphorylation of LAT [16], although the phosphorylation state of individual tyrosines in...

Ngày tải lên: 29/03/2014, 08:20

... cadmium-induced ERK activation diminishes cell death, whereas MKP3 exogenous expression diminishes cell death following cadmium intoxication (P Martin and P Pognonec, unpublished data) This sustained ... in NIH3T3 cells [40] and in astrocytes This increase in turn leads to an increase in ROS and to mitochondrial impairment [41] Pretreatment with a calcium chelator reduced ROS production and increased ... lead to cell death in certain systems, but that cadmium, in addition to caspase activation, triggers other parallel pathways that also culminate in cell death This could explain why ERK inhibitors,...

Ngày tải lên: 29/03/2014, 08:20