carcinoma clone 15 hac15 cell line and related clones

... after 2–3 weeks and re-cloned by serial dilution The stable cells transfected with sense and antisense PKB were named S-PKB/H7721 and AS-PKB/H7721 Cell culture and treatment Cells were cultured ... H7721 cells; Ins, H7721 cells treated with nM insulin; LY294002, H7721 cells treated with 15 lM LY294002; LY294002 + Ins, H7721 cells treated with both LY294002 and insulin; Mock, H7721 cells ... both cell migration and invasion were reduced to about 45% of the UnC level These two parameters also declined to 41.4% and 52.5% in AS-PKB/ H7721 cells when compared with the mock cells, and...

Ngày tải lên: 21/02/2014, 00:20

... purchased from Promega Cell culture The human hepatocellular carcinoma cell line, QGY-7703, was kindly provided by D Lu (Dept of Neurology, Stanford University, Palo Alto, CA, USA) The cells were maintained ... dithiothreitol and protease inhibitor cocktail added before use) for 15 on ice Cell lysates were centrifuged at 16 000 g for and the supernatants mixed with · Laemmli buffer and resolved by SDS–PAGE (15% ... containing the extracellular domain (amino acids 114–281) of the human liver cancer cell line, QGY-7703 [27], we treated the cells with increasing concentrations of TRAIL and then analysed cell viability...

Ngày tải lên: 23/03/2014, 17:22

... mediated predominantly by CAD and partially by other nucleases Page of Methods Cell lines NPC cell lines SUNE1 and HONE1, as well as the EBV genome-positive marmoset cell line, B95-8 (gifts from Prof ... other hand, cells transfected with LMP1 expression plasmids (Fig 4C, lanes and 4) showed very distinct and intense bands of sizes smaller than kb DNA sequencing of these bands (600 bp and 300 ... 11 12 13 14 15 16 17 18 Acknowledgements We would like to thank Prof Dr Choon-Kook Sam for the NPC cell lines and the EBV genome-positive marmoset cell line, B95-8; Dr Eng-Lai Tan and Prof Dr...

Ngày tải lên: 10/08/2014, 05:21

... characterization cHCC cell line Histomorphological characterization cHCC cell line A) Solid cell clusters of large epithelial cells with papillary projections at the periphery and extensive central ... smaller clusters and not as single cells The cell clusters were collected, fixed and handled as described As shown in Figure 2, histology revealed solid cell clusters of large epithelial cells, with ... widely used human hepatocyte cell line HepG2 (enzyme activity of HepG2 was set at 100%), and the commonly used canine kidney cell line MDCK The results showed the cHCC cell line produced 25% of the...

Ngày tải lên: 13/08/2014, 13:20

... growthpromoting and antiapoptotic factor in human hepatocellular carcinoma cells [15] Moreover, a recent study has suggested that miR-27a functions as an oncogenic miRNA in gastric adenocarcinoma cells ... gastric cancer growth L.-H Zhu et al Cell culture and transfection Human gastric adenocarcinoma cell line MGC803 was maintained in RPMI1640 (GIBCO BRL, Grand Island, NY, USA) supplemented with 10% ... the gastric adenocarcinoma cell line MGC803, providing the first proofof-concept that there is a potential link between the tumor promoter miR-23a and gastric adenocarcinoma cell proliferation...

Ngày tải lên: 18/02/2014, 04:20

... epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II, CD1d, ICAM-1 and the novel ... harvested and acquired immediately in a FACSCalibur and analyzed using the CELLQUEST software Histogram shows DCFH fluorescence in HepG2 cells grown without (thin line, – Fe) or with (thick line, ... anti-(mouse Igs) Cells were acquired immediately in a FACSCalibur and analyzed using CELLQUEST Histogram shows cell surface expression of acrolein adducts in HepG2 cells cultured without (thin line, –...

Ngày tải lên: 19/02/2014, 16:20

... action of extracellular nucleotides on the human lung adenocarcinoma cell line LXF-289, which is derived from solid lung adenocarcinoma Extracellular nucleotides strongly attenuated the cell cycle ... jB1 (NF-jB1) and the MAPKs extracellular signal -related kinase ⁄ (ERK1 ⁄ 2) and p38 Our findings underline the importance of extracellular nucleotides in the specific modulation of cell proliferation ... inhibition and apoptosis induced by P2Y2 receptors in human colorectal carcinoma cells: involvement of intracellular calcium and cyclic adenosine monophosphate Int J Colorectal Dis 16, 154 –166 15 Rapaport...

Ngày tải lên: 07/03/2014, 12:20

... Synthesis and transfer of Man5GlcNAc2 species in the MI8-5 cell line We have demonstrated previously that MI8-5 CHO cells are deficient in Glc-P-Dol-dependent glucosyltransferase I and that the ... of LLO and oligomannoside species bound to newly synthesized glycoproteins during Glc deprivation of MI8-5 cells MI8-5 cells were preincubated in 0.175 mM Glc for 20, 40 and 120 min, and then ... lipid intermediates depends on several cellular and extracellular variables The Glc concentration in the extracellular medium [20], the capacity of the cell to synthesize the different metabolic...

Ngày tải lên: 16/03/2014, 16:20

... N-procollagen and if types IX and XI collagens were also incorporated, in order to understand better the temporal sequence and mechanism of assembly Materials and methods Cell culture The RCS-LTC cell line ... tumor cells (Fig 1B) The results of radiolabeling with [3H]proline showed that the cultured RCS-LTC cells continued actively to synthesize and incorporate type II N-procollagen, and types IX and ... lCiÆmL)1 [3H]proline, 10 lgÆmL)1 ascorbate, 100 lgÆmL)1 bAPN, and incubation was continued for a further 24 h The medium was then removed and the cell layer extracted with 0 .15 M potassium phosphate...

Ngày tải lên: 31/03/2014, 07:20

... features of DRG cells present in F-11 cells such as l- and d-opioid receptors, receptors for prostaglandin and bradykinin, and voltage-sensitive calcium channels Also F-11 cells synthesize and release ... than fibroblast- or kidney-derived cell lines VR1 was not endogenously expressed in F-11 cells These cells, formerly generated and characterized by Platika et al [7] and Francel et al [8], have been ... codon for P761 and a stop codon The accuracy of the resulting new plasmid pcDNA3.1/rVRL-1 was confirmed by DNA sequencing of both strands Cell culture and transfection of F-11 cells F-11 cells (obtained...

Ngày tải lên: 31/03/2014, 07:20

... cells, twelve cell clones with stably expressed ABCC2shRNA were picked, cultured and analyzed separately ABCC2 mRNA expression in these cell clones was compared to that in parent CNE2 cells and ... of the selected cell clones was determined by western blot As shown in Fig 1C and 1D, ABCC2 protein expression of both cell clones, CNE2/shABCC2-1 and -2, was decreased by 76% and 74%, respectively ... molecules across the cellular membrane, and are confirmed to be associated with anticancer drug transporter [5,6] Cell lines and animals The human NPC cell lines CNE1, CNE2, 5–8F, 6–10B, and HONE1 were...

Ngày tải lên: 18/06/2014, 15:20

... pancreatic tumor cell line Cell lines The AR42J cell line, derived from a rat exocrine pancreas neuroendocrine tumor, was used Cells were obtained from the European Collection of Cell Cultures and were ... fitting and provided five parameters: the transport parameters for tracer into and out of the cell, K1 and k2, the parameters for phosphorylation and dephosphorylation of intracellular tracer, k3 and ... with GIST and recurrent gliomas [14 15] The expression of GRP receptor in AR42J cell line has been reported by other groups [16–17] So far, the expression of integrin ανβ3 in AR42J cell line has...

Ngày tải lên: 18/06/2014, 18:20

... http://www.translational-medicine.com/content/9/1/97 Cell lines and culture conditions The human colorectal cancer cell lines LoVo, SW480, HT29, HCT116 and LS174T were maintained in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented ... inhibited cell migration and invasion of CRC cell lines (A) Monolayers of cells transfected with CK2a-specific siRNAs were wounded by scraping, and wound closure was followed at 0, 12, and 24 h ... five CRC cell lines GAPDH expression was used as a loading control CK2a regulates growth, proliferation and senescence of CRC cell lines Because the process of tumorigenesis is closely correlated...

Ngày tải lên: 18/06/2014, 19:20

... vitro and in vivo Genes & Dev 2: 1627-1638 Imaging Cellular Metabolism 203 Hernandez-Verdun, D., Roussel P & Gebranes-Younes J 2002 Emerging concepts of nucleolar assembly J Cell Sci 115: 2265-2270 ... Tenley N., Wu C., Tian H., Wang Y & Lee Z 2011 PET imaging of hepatocellular carcinoma with 18F-fluoroethylcholine and 11C-choline Eur J Nucl Med Mol Imaging 38: 1248-56 Kubota, K., Furumoto S., ... therapy: oncogene and nononcogene addiction Cell 136: 823-837 Manders, E.M.M., Kimura H & Cook P.R 1999 Direct imaging of DNA in living cells reveals the dynamics of chromosome formation J Cell Biol...

Ngày tải lên: 22/06/2014, 02:20

... extracellular matrix and mediate endothelial cells adhesion, migration, and invasion Endothelial cells (ECs) also express RTK (i.e., the FGF and PDGF receptors) that are found on many other cell types ... migration, and enhanced survival of endothelial cells, as well as regulation of the recruitment of perivascular cells and bloodborne circulating endothelial precursors and hematopoietic stem cells ... branch in random ways This leads to uneven blood flow within the tumor, with areas of acidosis and hypoxia (which stimulate release of angiogenic factors) and high intratumoral pressures...

Ngày tải lên: 07/07/2014, 01:20

... (both Mycobacterium tuberculosis and M avium), fungi, or HIV, and in sarcoidosis Intracellular lipid deposition in Gaucher disease and obliteration of the marrow space related to absence of osteoclast ... myelosuppression (worsening thrombocytopenia and neutropenia, necessitating blood count monitoring) and an increased risk of deep vein thrombosis and pulmonary embolism Other treatments for MDS ... and hairy cell leukemia The pathophysiology has three distinct features: proliferation of fibroblasts in the marrow space (myelofibrosis); the extension of hematopoiesis into the long bones and...

Ngày tải lên: 07/07/2014, 04:20

... vitro cellular radiosensitivity, heavy ion biological effectiveness, and responses to chemotherapy agents for a sacral chordoma cell line Methods Cell lines and culture conditions The chordoma cell ... of cell lines A) Growth curves for three cell lines Black reverse triangle indicates HeLa cells, white circle indicates U87-MG glioma cells, and black circle presents chordoma origin U-CH1-N cells ... U-CH1-N cells after high LET heavy-ions A) X-rays and carbon-ions (LET 13, 30, 50, and 70 keV/μm), B) X-rays and neon-ions (LET 13, 70, and 120 keV/μm), C) X-rays and silicon-ions (LET 55, 150 , and...

Ngày tải lên: 09/08/2014, 09:21

... SOX9 and green fluorescent protein (GFP), at a titre of × 106 [14] After three repeated transductions, more than 90% of the cells were transduced and the cells were designated SOX9-SW1353 Cells ... knee joints and maintained in culture in medium (as above) [14] Cells were harvested for gene-expression analysis within the first week of culture (P0) and after and passages (P1 and P2) HACs ... authors read and approved the final manuscript Acknowledgements The authors wish to thank Andrew Hayes and Leo Zeef for technical and analytical assistance with the microarray study and to the...

Ngày tải lên: 09/08/2014, 10:21