car wash simulation with simulation activity
Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... wild-type hMnSOD, with an accompanying 10-fold drop in catalytic activity [23] Interestingly, replacement of Gln143 with a number of different residues, even the conservative replacement with Asn, resulted ... amide ˚ of Asn73 is near to (3.1 A) and possibly hydrogen bonded with the side chain carbonyl of Gln143 With the substitution of Gln143 with Ala, this favorable interaction between Gln143 and Asn73 ... hydrogen bonded with buried water molecules that are not seen in the crystal structure However, the backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide...
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Báo cáo khoa học: EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine⁄threonine kinases and phosphatase in Mycobacterium tuberculosis doc
Ngày tải lên :
16/03/2014, 14:20
... phosphorylation-dependent ATPase activity is associated with EmbR Dephosphorylation of EmbR by Mstp reduces its ATPase activity, interaction with STPKs and DNA-binding activity towards promoter regions ... bound to His-EmbR, (2.5 lg each) were incubated with Mstp (1 lg) for different time periods After incubation, the beads were washed twice with wash buffer to remove liberated 32Pi and the proteins ... incubated with a soluble fraction (5 lg of protein) of E coli cells expressing EmbR, at 25 °C for h in mL of NaCl ⁄ Pi buffer The protein–resin complex was washed six times with mL of NaCl ⁄ Pi each wash...
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Báo cáo khoa học: Brain succinic semialdehyde dehydrogenase Reactions of sulfhydryl residues connected with catalytic activity pdf
Ngày tải lên :
16/03/2014, 18:20
... react with cysteine via a SN2 reaction mechanism to give the corresponding carboxamidomethyl derivatives The reaction of haloacetamide derivatives with cysteine is 20–100 times as rapid as with ... the catalytic activity of SSADH, was examined by reacting the enzyme with DABIA DABIA, a chromophoric reagent, was chosen for our study for the following reasons: (a) it reacts with the SH groups ... Incubation of SSADH with increasing concentrations of DABIA resulted in a progressive decrease in its enzymatic activity (Fig 1) This inactivation followed pseudo firstorder kinetics with concentrations...
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Báo cáo khoa học: NtKTI1, a Kunitz trypsin inhibitor with antifungal activity from Nicotiana tabacum, plays an important role in tobacco’s defense response pot
Ngày tải lên :
23/03/2014, 03:20
... uninduced E coli extraction with NtKTI1 construct without putative signal peptide; lane 4, soluble sample protein from induced E coli extraction with NtKTI1 construct without putative signal peptide ... The antifungal activity towards R solani was prominent (Fig 4A), with antifungal action clearly observed 24 h after loading the samples Meanwhile, NtKTI1 also showed moderate activity against ... displays in vitro antifungal activity as a trypsin inhibitor To elucidate the functional identity of the NtKTI1 gene, we produced an N-terminally His-tagged protein with and without the predicted signal...
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Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus pptx
Ngày tải lên :
29/03/2014, 08:20
... 27 cm; GE Healthcare) equilibrated with 25 mm imidazole-HC1 (pH 7.4) The fraction was eluted with polybuffer 74 (pH 4.0), diluted 12-fold with de-aerated water and further eluted with 0.5 m NaCl ... followed by elution with 50 mm phosphate buffer and 10 mm phosphate buffer Antibacterial activity was assayed using the growth-inhibition plate method The fractions with antibacterial activity were ... Flow high sub (1.0 · 25 cm; GE Healthcare UK Ltd., Little Chalfont, Bucks, UK), equilibrated previously with the same buffer, and the column was then washed with the buffer The flow rate of the...
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Synthesis and Evaluation of Pyrazine Derivatives with Herbicidal Activity
Ngày tải lên :
02/07/2014, 12:48
... groups containing to carbon atoms and B represents an ethylamino, n-propylamino, n- or iso-butylamino, 1carboxyethylamino, 1-carboxy-n-propylamino, 1-carboxy-iso-butylamino, 1-carboxy-npentylamino ... results are in accordance with those obtained with 2-alkylsulfanylpyridine-4-carbothioamides (Kráľová et al., 1997) Similar study with anilides of 2-alkylpyridine-4-carboxylic acids has shown ... alkylpyrazine compounds (Fig 3, X) with plant growth regulating activity, where R1 is C1-C4 alkyl optionally substituted with halogen or cyclopropyl, optionally substituted with C1-C4 alkyl; R2 is C1-C8...
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Báo cáo toán học: "Hard Squares with Negative Activity and Rhombus Tilings of the Plane" pdf
Ngày tải lên :
07/08/2014, 13:21
... y2 (σ) to be minimal with this property (with respect to some total order of σ(2) ) We claim that we may add or delete z1 := se2 (y2 ) without ending up outside Λ2,2 (K) and without altering the ... be maximal with this property Let σ ∈ Ω1 (a) By construction, there exists an element x in σ(3i) \ (se)∞ (σ) such that n3 w3 (x) ∈ σ Let / x := x(σ) be maximal with this property (with respect ... with their dimer counterparts, and for all but the first family, the growth at z = −1 appears to be exponential Acknowledgment This work was carried out at the Technical University of Berlin within...
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Báo cáo toán học: "Hard Squares with Negative Activity on Cylinders with Odd Circumference" ppt
Ngày tải lên :
08/08/2014, 01:20
... paired with odd sets, leaving us with the conclusion that Z(Q2 ) is indeed zero whenever n is odd the electronic journal of combinatorics 16(2) (2009), #R5 Reducing Q2 to the family Λ of sets σ with ... [8] J Jonsson, Hard squares with negative activity and rhombus tilings of the plane, Electronic J Combin 13 (2006), no 1, #R67 [9] J Jonsson, Hard squares on grids with diagonal boundary conditions, ... statistical mechanics This model deals with configurations of non-overlapping (“hard”) squares in R2 such that the four corners of any square in the configuration coincide with the four neighbors (x, y...
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Báo cáo y học: "Serum levels of autoantibodies against monomeric C-reactive protein are correlated with disease activity in systemic lupus erythematosus" potx
Ngày tải lên :
09/08/2014, 01:23
... Eleven patient sera with strong reactivity regarding antinucleosome antibodies as judged by our assay were used for blocking experiments The sera were preincubated overnight with increasing concentrations ... Labs, Chantilly, VA, USA) were coated overnight at room temperature with native human CRP (Sigma, St Louis, MO, USA) in carbonate–bicarbonate buffer (pH 9.6) at a concentration of 1.0 µg/ml Such binding ... at flare Patients with active kidney involvement at the present flare are indicated by filled circles, patients without kidney involvement by open circles Four of four patients with ongoing nephritis...
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Báo cáo y học: "Association of elevated transcript levels of interferon-inducible chemokines with disease activity and organ damage in systemic lupus erythematosus patients" docx
Ngày tải lên :
09/08/2014, 13:22
... patients with different levels of disease activity, as assessed using SLEDAI-2K and the level of complement C3 SLE patients were divided into those with stable disease (SLEDAI-2K score to 4), those with ... patients without a flare (P = 0.029; Figure 2a) Chemokine scores were positively correlated with SLEDAI-2K scores (r = 0.34, P < 0.005; Figure 2b) C3 level is also an indicator of disease activity, with ... higher in patients with active LN than in those with inactive LN or without LN (P = 0.014 and P = 0.002, respectively; Figure 3b), indicating that chemokine scores are associated with ongoing renal...
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Báo cáo y học: "Collagen-specific T-cell repertoire in blood and synovial fluid varies with disease activity in early rheumatoid arthritis" pot
Ngày tải lên :
09/08/2014, 13:22
... accordance with the manufacturer's instructions CD19-negatively sorted cells were enriched in CD45RA+ and CD45RA- cells by labelling them with an anti-CD45RA MACS™ beads, in accordance with the ... Cloning® kit (Invitrogen, Carlsbad, CA, USA), in accordance with the manufacturer's instructions Transformed Escherichia coli were grown in ml LB medium supplemented with ampicillin, and plasmids ... after stimulation with the subdominant epitope huCollp289–303 In these patients, overall we identified 26 rearrangements expanded by stimulation with huCollp261–273 but not with huCollp289–303...
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Báo cáo y học: "Increased production of soluble CTLA-4 in patients with spondylarthropathies correlates with disease activity" pps
Ngày tải lên :
09/08/2014, 14:22
... serum sCTLA-4 in patients with SpA, compared with levels observed in patients with RA or in healthy volunteers Circulating sCTLA-4 correlated with clinical index of disease activity BASDAI and CRP ... spondylarthropathies (SpA,(HC, 165), with Serum concentrationswith = 71) and healthy Lymphocyte Antigen- (sCTLA-4) in patients with spondylarthropathies (SpA, n = 165), with rheumatoid arthritis (RA, ... factors Patients with systemic lupus erythematosus In order to have another comparative group (with previously determined elevated serum sCTLA-4 levels) [15,16], 14 patients with SLE (13 females,...
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Báo cáo y học: "Markers of B-lymphocyte activation are elevated in patients with early rheumatoid arthritis and correlated with disease activity in the ESPOIR cohort" pdf
Ngày tải lên :
09/08/2014, 14:22
... test was used to compare continuous data between patients with RA and patients with UA or, within the RA group, between patients with or without RF, anti-CCP, or radiological erosions The chi-square ... activation are higher in patients with early RA than in patients with undifferentiated arthritis (UA) In addition, their increase is correlated with disease activity but is independent of serum ... activation are elevated in patients with early RA compared with undifferentiated early arthritis They also show that markers of B-cell activation are correlated with disease activity Lastly, they indicate...
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Báo cáo y học: "Increased levels of circulating microparticles in primary Sjögren''''s syndrome, systemic lupus erythematosus and rheumatoid arthritis and relation with disease activity" pdf
Ngày tải lên :
09/08/2014, 14:22
... or metabolic diseases Cardiovascular risk factors (diabetes, smoking, arterial hypertension, hyperlipidemia) were noted in three patients with pSS, one with SLE, and six with RA Of note, at the ... Correlation of MP level with disease activity of pSS and other AIDs As MPs can reflect the state of cellular stimulation, we hypothesized that the level of MPs could be associated with AID activity Platelet ... concomitantly with MP acquisition in the specific gate (b to d) Gated MPs alone (b) without annexin V addition, (c) stained with annexin V FITC in a calcium-specific buffer, and (d) stained with annexin...
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báo cáo khoa học: "Nodulin 41, a novel late nodulin of common bean with peptidase activity" pot
Ngày tải lên :
11/08/2014, 11:21
... h at room temperature with 10 μg/ml of PvNod41 dissolved in TBST [TBS, 0.1% (v/v) Triton X100] After washing three times for 15 each with TBST, the blots were incubated with anti-PvNod41 antiserum ... copolymerized with 0.15% gelatin The gel was run at constant voltage (120 V) for 1.5 h at room temperature SDS was removed by washing the gel three times with 50 ml of 20 mM Tris-HCl, pH 4.5 with 0.1% ... at room temperature with anti-PvNod41 antiserum diluted 1:5000 in TBST After washing three times for 15 each with TBST, the blots were incubated for h at room temperature with goat anti-mouse...
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báo cáo khoa học: " Biological activity of a genetically modified BMP-2 variant with inhibitory activity" potx
Ngày tải lên :
11/08/2014, 20:20
... BMPR-II [9] Different BMP-2 mutants with alterated binding epitopes were developed by Kirsch et al The in vitro evaluation of their biological activity, using ALP activity as a marker, revealed alterated ... method described by Kuberasampath and Ridge [12] The cylindric carriers with a diameter of mm and a length of 10 mm were autoclaved, soaked with the protein solution and lyophilized Animal studies ... split animal design Control specimens (carriers coupled with μg rhBMP-2) were implanted into prepared muscle cavities on the left side Test specimens loaded with same dose rhBMP-2 (5 μg) as well...
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Báo cáo y học: "Enhanced late-outgrowth circulating endothelial progenitor cell levels in rheumatoid arthritis and correlation with disease activity" doc
Ngày tải lên :
12/08/2014, 11:23
... arthritis (RA) with disease activity Correlation between EPC counts and disease activity (a) and association of lower EPC levels in different groups of RA patients according to disease activity score ... comparison with other works Neverthe- less, disease duration was never reported to be associated with EPC levels and thus may not account for our findings We excluded RA patients with cardiovascular ... were cardiovascular events and conventional cardiovascular risk factors (diabetes, hypertension, and past medical history of coronary artery disease and smoking) except for three RA patients with...
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Báo cáo y học: " Expression of K2P5.1 potassium channels on CD4+ T lymphocytes correlates with disease activity in rheumatoid arthritis patients" potx
Ngày tải lên :
12/08/2014, 15:22
... patients with low disease activity (2.6
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Báo cáo y học: "Interferon-lambda1 induces peripheral blood mononuclear cell-derived chemokines secretion in patients with systemic lupus erythematosus: its correlation with disease activity" potx
Ngày tải lên :
12/08/2014, 15:23
... positively with SLEDAI scores and negatively with C3 levels Compared with patients without lupus nephritis and those with inactive lupus nephritis, chemokine scores were increased in patients with ... with normal controls In addition, they had a positive relation with SLEDAI Furthermore, in SLE patients with renal involvement, IP-10 and IL-8 levels were positively correlated with disease activity ... SLE patients with active disease and inactive disease as well as NC (a) IFNl1 mRNA levels were significantly elevated in SLE patients with active disease (n = 27) compared with those with inactive...
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Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals" ppt
Ngày tải lên :
12/08/2014, 23:22
... other than rodents and primates but possessing a placenta with a related architecture, i.e with a syncytiotrophoblast layer in direct contact with maternal blood at the maternal-fetal interface, have ... fide syncytin gene, with high levels of expression in the placenta and very limited expression in other tissues Expression in the placenta decreases with gestational age, with a four-fold reduction ... Figure Fusogenic activity of syncytin-Ory1 Fusogenic activity of syncytin-Ory1 (A) Assay for cell-cell fusion mediated by syncytin-Ory1 The indicated cell lines were transfected with an expression...
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