capsids nucleic acids and envelopes

... ORFs UL56 and UL55 (gB), and two thirds of the ORF UL54 (DPOL) (Figure 6B) The nucleotide and amino acid sequence differences of CgueCMV-1.1 and CgueCMV-1.2 were 0.6% and 0.5% (UL56), 20% and 8% ... found in the kidney and brain and the other in liver and mucosa (CMV-1 and CMV-2, respectively) They had a nucleotide sequence identity of 82% With LD-PCR, the CMV-1 gB sequence and the CgueCMV-1 ... CgueCMV-1.1 and CgueCMV-1.2 (B) Pairwise alignments of the UL56 and gB proteins and the partial DPOL proteins of CgueCMV-1.1 and CgueCMV-1.2 are shown Dots represent identical amino acids, dashes...

Ngày tải lên: 18/06/2014, 18:20

... Detection of nucleic acids Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic acids ... glassy and spherical carbon powder and MWCNTs had the highest sensitivity to nucleic acids Based on these results, nanocarbon II electrodes were further utilized for detection of both the nucleic acids ... of (D) single strand oligonucleotide influenza (13 μg/mL), and (E) double strand genomic DNA (15 μg/mL) In insets: signals of all nucleic acid bases after baseline correction and smoothing of...

Ngày tải lên: 21/06/2014, 03:20

... molecule ligand or ligands (for example gluthatione), as a nucleic acid label, and an appropriate receptor or protein fragment which binds the ligand as part of the protein vector and which is ... by joining the untagged SAPV and modified AFP by PCR using primers T7-F and TFTER-R and a mixture of the SAPV fragment (M13F/SA-11R PCR fragment) and of the linear and modified AFP fragment (AFP-1F/SA-8R ... Kuntz ID, Birken S, Axel R and Cantor CR Molecular cloning and nucleotide sequence of the streptavidin gene Nucleic Acids Res 1986, 14:1871-1882 Blake KR, Murakami A and Miller PS Inhibition of...

Ngày tải lên: 11/08/2014, 00:21

... specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses Retrovirology 2010 7:112 Submit your next manuscript to BioMed Central and take full advantage ... studies of XMRV: (i) MLV-encoding nucleic acids present in commercial PCR reagents, (ii) trace amounts of mouse genomic DNA in human blood and tissue samples, and (iii) DNA or RNA from human tumor ... AI27757) and Public Health Service grants R01 AI060466 and R37 AI47734 I thank Drs Geoff Gottlieb, Jim Mullins, John Mittler and Mary Campbell (UW) and Dr Dusty Miller (Fred Hutchinson Cancer Research...

Ngày tải lên: 13/08/2014, 01:20

... REVIEW .1 1.1 Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization .1 1.1.2 Label-free detection of nucleic acids 1.1.2.1 Electrochemical ... simple, low cost and ultrasensitive electrochemical nucleic acids detection in portable devices, POC and early disease diagnostic applications (II) bDNA-amplified electrochemical nucleic acids assay ... of nucleic acids hybridization and detection on a solid surface The signal transduction strategies of nucleic acids hybridization can be broadly divided into two main categories: label-free and...

Ngày tải lên: 12/09/2015, 09:28

... Moderate Weak Structure of Acids • oxy acids have acid hydrogens attached to an oxygen atom H2SO4, HNO3 Tro, Chemistry: A Molecular Approach Structure of Acids • carboxylic acids have COOH group ... to produce OH- ions and cations ionic substances dissociate in water NaOH(aq) → Na+(aq) + OH–(aq) • acids ionize in water to produce H+ ions and anions  because molecular acids are not made ... the Brønsted-Lowry Acids and Bases and Their Conjugates in the Reaction H2SO4 + H2O ⇔ HSO4– + H3O+ When the H2SO4 becomes HSO4−, it lost an H+ − so H2SO4 must be the acid and HSO4− its conjugate...

Ngày tải lên: 28/11/2013, 01:12

... racemic at carbon 3, and the synthetic (3-R,S)-methylhexadecanoic and (3-R,S)-methylheptadecanoic acids, have been used It has been shown that the synthetic 3-methyl-branched fatty acids are metabolized ... van de Vlaamse GemeenschapÕ (GOA 94/98–12 and GOA 99/03–09) and from the ÔFonds voor Wetenschappelijk Onderzoek-VlaanderenÕ (G-0239.98, G.0164.96 N and G.0115.02) V.F was supported by a fellowship ... phytanic acid and 3-methyl-adipic acids in patients with Adult Refsum’s disease In Peroxisomal Disorders and Regulation of Genes, 25–28 September 2002, Belgian Society for Cell and Developmental...

Ngày tải lên: 08/03/2014, 02:20

... (depending on the sugar they contain) 1- Ribonucleic acids (RNA) The pentose sugar is Ribose (has a hydroxyl group in the 3rd carbon OH) 2- Deoxyribonucleic acids (DNA) The pentose sugar is Deoxyribose ... Adenine, Thymine, Cytosine, Guanine 3- Double-stranded helix arrangement  RNA 1- Ribose sugar 2- Bases: Adenine, Uracyl, Cytosine, Guanine 4- Single stranded The Double Helix (DNA) Structural model: ... the other: A -T , C -G Due to this specificity, a certain base on one strand indicates a certain base in the other  The strands intertwine, forming a doublehelix that winds around a central axis...

Ngày tải lên: 13/03/2014, 16:34

... (depending on the sugar they contain) 1- Ribonucleic acids (RNA) The pentose sugar is Ribose (has a hydroxyl group in the 3rd carbon -OH) 2- Deoxyribonucleic acids (DNA) The pentose sugar is Deoxyribose ... Adenine, Thymine, Cytosine, Guanine 3- Double-stranded helix arrangement  RNA 1- Ribose sugar 2- Bases: Adenine, Uracyl, Cytosine, Guanine 4- Single stranded The Double Helix (DNA) Structural model: ... the other: A -T , C -G Due to this specificity, a certain base on one strand indicates a certain base in the other  The strands intertwine, forming a double-helix that winds around a central axis...

Ngày tải lên: 13/03/2014, 17:04

... cellular activity Primary Structure of Nucleic Acids • The primary structure of a nucleic acid is the nucleotide sequence • The nucleotides in nucleic acids are joined by phosphodiester bonds ... strands is very specific • The complimentary base pairs are A-T and G-C - two hydrogen bonds form between A and T - three hydrogen bonds form between G and C • Each pair consists of a purine and ... A, C, G, and U are linked by 3’-5’ ester bonds between ribose and phosphate Example of DNA Primary Structure • In DNA, A, C, G, and T are linked by 3’-5’ ester bonds between deoxyribose and phosphate...

Ngày tải lên: 13/03/2014, 17:32

... ()61TTATTT)56 and )37TTATTT)32), GLU2 [)506TTATTT)501 and )90TTATTT)85 ()strand)], GLT ()311TATAAAT)305), carA ()277TATATAA)271 and )188TTATTT)183), and carB [)361TTATTT)356 and )143TTATTT)138 ()strand)] ... The high levels of glutamine and glutamate (nitrogen-rich ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc ... )220 )217, and )129 )216), GLU2 ()223 )220, )218 )215, and )139 )216), GLT ()310 )307, )28 )25, and )24 )21), carA ()237 )234, )151 )148, and )115 )112), and carB ()405 )402 and )79 ...

Ngày tải lên: 30/03/2014, 01:20

... Electrophoresis of Nucleic Acids, Vol 1: Introduction to the Capillary Electrophoresis of Nucleic Acids Edited by: K R Mitchelson and J Cheng © Humana Press Inc., Totowa, NJ 27 28 Slater, Desruisseaux, and ... electroosmosis, and zone dispersion, in Capillary Electrophoresis Theory and Practice (Grossman, P D and Colburn, J C., eds.), Academic Press, San Diego, pp 3–43 Meistermann, L and Tinland, B (1998) Band ... Wilding, P., and Ramsey, J M (2001) DOP-PCR amplification of whole genomic DNA and microchipbased capillary electrophoresis, in Capillary Electrophoresis of Nucleic Acids, Vol (Mitchelson, K R and Cheng,...

Ngày tải lên: 10/04/2014, 11:11

... markers and size standard can be discriminated by the color Electropherograms for injections 1, 100, 200, and 300 are shown: (A), The 100- and 500-bp peaks of the size standard are highlighted and ... H., and Foret, F (1995) Capillary electrophoresis of proteins and nucleic acids Annu Rev Biophys Biomol Struct 24, 579–610 Olivera, B M., Baine, P., and Davidson, N (1964) Electrophoresis of nucleic ... Electrophoresis of nucleic acids Biopolymers 2, 245–257 Wehr, T., Zhu, M., and Mao, D T (2001) Sieving matrix selection, in Capillary Electrophoresis of Nucleic Acids, Vol (Mitchelson, K R., and Cheng, J.,...

Ngày tải lên: 10/04/2014, 11:11

... absorbance at both 595 and 650 nm, using the bg/mL standard as a blank Plot a standard curve of absorbance at 595 nm mmus absorbance at 650 nm as a functron of mrtral DNA concentration and then use the ... 26137 and CA 21797 References Zimmerman, S B., and Sandeen, G (1966) The ribonuclease activity of crystallized pancreatic deoxyribonuclease Anal Bmchem 14, 269-277 Maxwell, I H , Maxwell, F , and ... component that gives a sharp band m a density gradient and can be resolved from the broader mam band of DNA m the gradrent The usual gradient material is CsCl m aqueous buffer and the Cs-’ ions form...

Ngày tải lên: 11/04/2014, 10:00

... transcription by peptide nucleic acid (PNA) at minimal ratios of PNA/RNA Nucleic Acids Res 25, 2167–2173 Lee, R., Kaushik, N., Modak, M J., Vinayak, R., and Pandey, V N (1998) Polyamide nucleic acid targeted ... 73–78 10 Nielsen, P E and Egholm, M., ed (1999) Peptide Nucleic Acids: Protocols and Applications Horizon Press, Wymondham, Norfolk, UK 18 Nielsen 11 Nielsen, P E (1999) Peptide nucleic acid A molecule ... Peptide nucleic acids as antibacterial agents via the antisense principle Expert Opin Invest Drugs 10, 331–341 15 Ray, A and Nordén, B (2000) Peptide nucleic acid (PNA): its medical and biotechnical...

Ngày tải lên: 11/04/2014, 10:02

... ‘Non-protein amino acids , alias ‘non-proteinogenic amino acids or ‘non-coded amino acids 1.9 Coded amino acids, non-natural amino acids and peptides in nutrition and food science and in human physiology ... Introduction 1.1 Sources and roles of amino acids and peptides More than 700 amino acids have been discovered in Nature and most of them are ␣-amino acids Bacteria, fungi and algae and other plants provide ... geological and extra-terrestrial distribution of amino acids 1.11 Amino acids in archaeology and in forensic science 1.12 Roles for amino acids in chemistry and in the life sciences 1.12.1 Amino acids...

Ngày tải lên: 04/06/2014, 16:05

... acids are electron pair acceptors and bases are electron pair donors acids are electron pair donors and bases are electron pair acceptors Q: A chemical ... ion is: monoprotic diprotic triprotic polyprotic Q: Vinegar, fruit juice, and cola are examples of: strong acids weak acids strong bases weak bases ...

Ngày tải lên: 05/07/2014, 03:00

... Extraction and analysis of phenolic acids Phenolic acid contents Phenolic acids were extracted as described by Cvikrová et al (1991) Briefly, free, ester-bound (released after alkaline hydrolysis) and ... a further significant rise in SG and CWE was determined The HPLC analyses indicated the presence of two cinnamic acid derivatives, p-coumaric and ferulic acids and of five benzoic acid derivatives ... (anisic, p-hydroxybenzoic, vanillic and syringic acids) in the Norway spruce cells, and there were no qualitative differences in the phenolic acid com- Extraction and analysis of stilbenes For the...

Ngày tải lên: 07/08/2014, 10:21