capillary electrophoresis techniques in biomedical analysis
Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants
... discontinuous electrolyte system 12 Chapter Introduction formed by the leading and terminating electrolytes The leading electrolyte contains ion with the high mobility, whereas the terminating electrolyte ... .55 Figure 2.3 Electrophoresis of ΦX174 DNA in Buffers containing Different Counter-ions 58 Figure 2.4 Electrophoresis of ΦX174 DNA in Buffers containing Different Co-ions ... resulting in different of CE, having many Electrophoresis migration velocities of ionic possible applications (CZE) species in the electrophoretic for small and large buffer contained in the capillary...
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Combining DEMO models with RAD's techniques in the analysis phase of software development process
... By using above five steps, the IAM can be developed It can help to improve the operation of the organization by redesigning and reengineering the business In other words, before building an information ... related transactions Capturing the business processes of the organization, it can be used as the starting point to design the supporting system for the organization Combining with IAM, the PM can ... Technique: The analysts fulfill the following table by using steps in the PM and requirements definition list There are six columns in the following table MAPPING TABLE Requirements: They are requirements...
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Development and application of liquid phase microextraction techniques in the analysis of environmental pollutants
... drugs such as amphetamines and phencyclidine in urine were investigated using LPME combined with GC analysis [57] The optimized method was capable of detecting drugs in urine at concentrations ... achieved with capillary LC analysis This method was also extended to the analysis of aromatic amines in water samples [89], phenoxy herbicides in bovine milk [90] and aminoalcohols in urine [91] Recently ... Mental Health Services Administration established cut-off values for preliminary testing Cocaine and cocaine metabolites in urine samples have also been investigated using LPME [58] 16 Recently,...
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New Techniques in the Analysis ofFoods
... gluten upon denaturation (heating) The dough is worked significantly resulting in an increased chance of protein-protein interaction resulting in aggregation and clumping of Figure Confocal microscopic ... polarize in a large alternating field 7' 19 In support of this statement, Bezruk et al 20 observed a diminishing of the induced-aggregate size with increases in the electric frequency According to ... using calf chymosin, bovine pepsin, porcine pepsin, and Rhizomucor miehei (formerly Mucor miehei) as coagulants' The calf chymosin cheese had the greatest melt and smallest stretch, the porcine...
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Parallel and distributed computing techniques in biomedical engineering
... of biomedical engineering, which is also known as bioengineering Of the many methods of engineering principles, computational and numerical methods have been receiving increasing emphasis in ... which is very common in research in computational fluid dynamics; and biomedical image processing, which is increasingly playing an essential role in research in diagnostic and clinical experiments ... role in the synthesis and integration of information The National University of Singapore combination of biological science research and engineering discipline has resulted in the fast growing...
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Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis potx
... avidin complex (avidin-biotin) Reprinted from [ks.uiuc.edu] Various proteins, e.g., bovine serum albumin, 1-acid glycoprotein, ovomucoid, avidin (Figure 2.28), transferring, pepsin, penicillin ... compounds in chiral CD cavity results in differences in stability of the formed complexes 30 Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis Compounds containing ... separation, continuing with sample preparation and finishing with detection The knowledge and findings from the review and research papers (involving also the author’s works) included here give an integral...
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On line pre concentration techniques in capillary electrophoresis for environmental analysis
... electric field Capillary electrophoresis (CE) is electrophoresis performed in a capillary tubing with typical internal diameter (i.d.) of 20-150 μm The principle underlying most kinds of electrophoresis ... 1.3.2 Sample Introduction Sample can be introduced into the capillary in two common ways in CE One is the hydrodynamic injection, and the other is electrokinetic injection In hydrodynamic injection, ... stacking neutral analytes in high-salt sample matrix with electrokinetic injection The analytes were injected into the capillary by EOF and was stacked at the inlet due to their partitioning into...
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Applications of capillary electrophoresis in the analysis of natural products
... caffeine, theobromine, and theophylline but in varying amounts They also have polyphenolic compounds; catechins including epigallocatechin; gallic acid; bioflavonoids; volatile oil; some vitamin ... that can be performed using same CE instrument Different modes can be achieved by simply changing the separation medium in the capillary or making a coating on the capillary internal wall Table 1.2 ... 146 VIII Chapter Introduction 1.1 Principles and historical perspective of Capillary Electrophoresis Since the introduction of capillary electrophoresis (CE) in 1980s by Jorgenson, the...
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Development of liquid phase microextraction techniques combined with chromatography and electrophoresis for applications in environmental analysis
... thicker coating However, the main drawback of this technique is that the introduction and desorption of extracting membrane in the GC injection liner is quite complicated PC-HFME was developed in our ... 1.7 ml/min The following temperature program was employed: initial temperature of 60 °C for min; increased at 10 °C/min to 260 °C, held for The injector temperature was 270 °C and all injections ... Effect of stirring in the sample solution 56 Figure 3-5 Effect of pumping rate 58 Figure 3-6 Effect of dwell time (a) dwell time in pumping programmed phase 2; (b) dwell time in pumping programmed...
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The development of acidic protein aptamers using capillary electrophoresis methods and their use in surface plasmon resonance
... sequence and binding affinities for each full hemoglobin aptamer using affinity capillary electrophoresis 87 Table 4.7 A summary of the estimated binding affinities of different proteins towards ... against different proteins The catalase protein shows a 100 fold increase in binding compared to the other proteins 174 Figure b.11 Electophoreogram of the initial haemoglobin library using capillary ... from combinatorial libraries against protein targets using kinetic capillary electrophoresis8 4 Protein adsorption onto the capillary surface is a challenging problem, meaning that for proteins with...
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Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies
... monitored in our water sources using analytical techniques In this study, capillary electrophoresis techniques were developed for the determination of cyanobacterial toxins (including microcystins, ... cylindrospermopsin and neurotoxins such as anatoxin-a, anatoxin-a(s) and saxitoxins, the marine toxin aplysiatoxin, debromoaplysiatoxin and lyngbyatoxin-a), and lipopolysaccharides (LPS) All are biotoxins ... (L) and arginine (R) in microcystin LR, arginine (R) and arginine (R) in microcystin RR, Leucine (L) and tryosine (Y) in microcystin LY etc Microcystins have commonly been isolated from Microcystis,...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 1
... by detecting the major components in aminoglycoside antibiotic mixtures Gentamicin was separated into several peaks in 15 minutes, and neomycin into three peaks even faster within minutes This ... Peter C Hauser, Determination of Tobramycin in Human Serum by Capillary Electrophoresis with Contactless Conductivity Detection, Electrophoresis, in press LingLing Yuan, HongPing Wei, HuaTao Feng, ... (Edwardsiella tarda) in fish species by capillary electrophoresis with blue light-emitting diode induced fluorescence, Electrophoresis, 25, 2004, 3139-3144 LingLing Yuan, HongPing Wei, Sam Fong...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 2
... Improving Sensitivity in CE One of the major challenges in capillary electrophoresis and other miniaturization 44 separation techniques is to maintain high detection sensitivity in the increasingly ... injection [6] In pressure injection, the inlet end of the capillary is immersed in the sample and the other end in the buffer Sample is introduced by either pressurizing the inlet vial containing the ... Sample Introduction and Concentration 1.4.1 Sample Introduction There are normally three main kinds of injection methods, which are pressure injection, siphoning injection and electrokinetic injection...
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Data mining techniques in gene expression data analysis
... exist some combinations of rows, X, such that I(X) = ∅ 2.3.2 Pruning Strategies In MineTopkRGS, top-k pruning is the main pruning strategy, and other pruning techniques first introduced in [21] are ... data which combines the discriminating powers of the emerging patterns of each class Unsupervised data mining methods mainly refer to clustering methods The clustering subroutine typically groups ... discriminating powers of the top k covering rule groups of each training sample To address the complex nonlinear and shifting-and-scaling correlations among genes ix in a subset of conditions, we introduce...
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Analysis of intact bacteria, bacterial DNA and mutagenic alkaloids by capillary electrophoresis
... Chapter Introduction 1.1 Electrophoresis 1.2 History of capillary electrophoresis 1.3 Basic principles of capillary electrophoresis 1.4 Different modes of capillary electrophoresis 19 1.5 Instrumentation ... pyrrolizidine alkaloids in traditional Chinese medicine Keywords: Intact bacteria, DNA, alkaloid, capillary electrophoresis VII Chapter Introduction Chapter Introduction 1.1 Electrophoresis Electrophoresis ... to maintain a constant and low conductivity in samples when using electrokinetic injection 1.5.2 Sample stacking As mentioned above, the injection volume is generally less than 1% of the capillary...
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Development of temporal phase analysis techniques in optical measurement
... of rotating the grating in x-y plane and moving it in z-direction (Jin et al 2000), and rotating the grating in x-z plane (Xie et al 1997) However, in shadow moiré technique, phase shifting is ... Analysis Carrier Fringe Technique Fourier analysis Temporal Phase Analysis Fringe Tracking Phase Scanning Method Fourier analysis Wavelet Wavelet Figure 1.1 Fringe analysis techniques applied ... phase analysis techniques and applying them to measurement of continuouslydeforming objects or low-frequency vibrating objects indicated in Fig 1.1 in red colour The objectives of this thesis include:...
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Synthesis of ionic liquids and their applications in capillary electrophoresis
... (MS), indicating their different combining modes in organic solvents which would partially relate to their behavior in CE A capillary electrophoresis (CE) method for determining the main impurity ... adjustable coordinating ability 1.1.4 Use in capillary electrophoresis With the increasing interests with this kind of new materials, some analysts expanded the application of ILs to capillary electrophoresis ... ILs in this emerging area 1.2 Capillary electrophoresis There has been tremendous growth of capillary electrophoresis (CE) in the past decades since the launch of the first commercial CE instrument...
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Complex field analysis of temporal and spatial techniques in digital holographic interferometry
... ‘holo’ meaning whole and ‘graphein’ meaning to write It is a clever method of combining interference and diffraction for recording the reconstructing the whole information contained in an optical ... with initially developed algorithms resembling the former fringe counting The introduction of the phase shifting methods of classic interferometric metrology into HI was a big step forward, making ... REVIEW 2π The processing of converting the interference phase modulo 2π into a continuous phase distribution is called phase unwrapping This can be defined in the following expression (Creath,...
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Emerging techniques in power system analysis Kỹ thuật mới nổi trong phân tích hệ thống điện
... specific emerging techniques for power system analysis These techniques include cascading failure analysis, data mining and its applications in power system analysis, grid computing, phasor measurement ... further reading on emerging techniques for power system analysis With the new initiatives and continuously evolving power industry, technology advances will continue and more emerging techniques ... quantify the increasing uncertainties in power systems and assist operators and planning in making objective decisions Various probabilistic analysis techniques are introduced in this chapter...
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