capillary electrophoresis for the detection of fragile x expanded alleles

... many proteins This ultimately Another drawback for the use of EDCHCl/sulfo-NHS for the formation of stable bioconjugates is the lack of specificity on the protein of interest As numerous amine functional ... [33] The use of QD-hydrazide (in contrast to QD-NH2) for bioconjugation with an oxidized IgG (IgG-CHO) increases the selectivity of the reaction However, there still remains the potential to form ... carboxylated QDs have been performed with the use of EDC alone [7] Despite the simplicity of a one-step reaction, the drawback involves a degree of uncontrollability during bioconjugation, forming...

Ngày tải lên: 11/08/2014, 00:22

... β-CD-NH2 and the backward movement of the neutral sample partitioned in the micelles of sodium dodecyl sulfate (SDS) Stability of the inclusion complexes formed between the carrier and the solute ... displacer plug was introduced before sample injection to reduce the length of the concentrated sample zone and increase the peak height by slowing down the forward movement of the neutral sample associated ... Publications……………………………………………………………………… xii~xiii Summary………………………………………………………………… ……….xiv~xvi Chapter Introduction……………………………………… …… 1~57 1.1 Basic Theory of Capillary Electrophoresis ……………………….… 1~6 1.2 Electroosmotic Flow (EOF)……………………………………………...

Ngày tải lên: 14/09/2015, 10:36

... Where, Δp: the pressure difference; r: the inner diameter of the capillary; 20 t: the injection time; η: the viscosity of the buffer; L: the total length of the capillary In the case of all hydrodynamic ... macromolecules The transport of the solutes through the 15 capillary is based on the charge of the macromolecules, but the separation is dependent on the molecular size Besides the gel forming medium, other ... suppliers The principle of UV detection in CE is the validity of the Lambert-Beer law [38, 39] UV detection is performed on column The optical path length is approximately defined by the ID of the...

Ngày tải lên: 14/09/2015, 10:37

... limits of detection of various modes of detection in MCE 46 Table 3.2 The parameters and their respective conditions in the stepwise optimization of the dimensions of the microchip detection ... even after it is deformed by the application of external forces Hence, soft lithography technique is commonly utilized for the microfabrication of such microchips35 The design of the fluid circuit ... applied, the mobile layer of buffer cations migrates towards the cathode As it does, it drags the bulk of the buffer solution along with it and thereby generating the EOF The strength of this EOF is...

Ngày tải lên: 02/10/2015, 12:56

... according to whether the patients had prior revascularization The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile ... coronary stenosis according to sex and age groups The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile range (IQR) ... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting...

Ngày tải lên: 03/11/2012, 10:58

... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts ... supported by the research project The research on the development of the total evaluation technique for the hazardous impacts by the chemical substances towards human and ecology (the project ... postlabeling method, etc.) Therefore, it is important to develop a simple rapid in vitro method for evaluating the generation of reactive oxygen species from a variety of genotoxic chemicals We developed...

Ngày tải lên: 05/09/2013, 08:40

... addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology would have the greatest potential benefit Finally, since 1/3 of the ... used for decades as an aid to the identification of mucosal abnormalities of the cervix as well as in the oral cavity It has been valued by surgeons as a useful way of demarcating the extent of ... reproducibility of the test At a minimum, the report should include an adequate description of the cohort of patients that were studied, a description of how the screening exam or test was performed and...

Ngày tải lên: 06/03/2014, 02:21

... drops of the creatine solution, three drops of the ethanolic solution of 1-naphthol and then two drops of the potassium hydroxide solution; shake after the addition of each reagent The formation of ... the results of the interpretation, indicate the presence or absence of Salmonella in a test portion of x g or x ml of product (see ISO 7218) See annex C for the precision data obtained from the ... leave for several minutes (approximately min) Add 0,25 ml of the reagent for detection of β -galactosidase and mix Replace the tube in the water bath set at 37 °C and leave for 24 h ± h, examining...

Ngày tải lên: 07/03/2014, 16:20

... monitor the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature ... one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to NO2 that are responsible for the high sensitivity associated with the promotion However, whether ... materials of WO3 promoted with wt.% metal or metal oxides for NO detection were then systematically screened It was found that some of these promoters were critical for improving the sensitivity [9] The...

Ngày tải lên: 19/03/2014, 16:47

... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... between the 833 bp of the ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87% nucleotide identity The 834 bp sequence of the ... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses...

Ngày tải lên: 18/06/2014, 22:20

... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... between the 833 bp of the ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87% nucleotide identity The 834 bp sequence of the ... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses...

Ngày tải lên: 20/06/2014, 04:20

... Parkinson disease, for the development of new sensitive drug screening methods and for the quantification of toxicodynamic and toxicokinetic effects Application of the MCC for the analysis of nanoparticleinduced ... previous exposures Therefore, the analysis and quantification of single-cell responses will provide important information on the toxicity of the tested materials The MCC-based method is therefore ... GC10 for 24 h After the exposure time, the GFP expression of the pIL8-GFP A549 cells in the microcavities was analyzed by fluorescence microscopy The percentage of GFP-expressing cells in the...

Ngày tải lên: 21/06/2014, 00:20

... R∼−1^∼a rx ^x rx x x x x x x x Σ (37) K n=0 Using (37) into (9), we deduce that a sufficient statistic for the previous detection problem is given by (31) for K’ = and can be written as OPT5(xv, K) ... statistical theory of detection, the optimal receiver for the detection of the K symbols an from both the K primary data xv(nT) and the K’ secondary data bTv(nT)’ consists in comparing to a threshold the ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors...

Ngày tải lên: 21/06/2014, 00:20

... Page of adjust the pH of the mixture to 9.1 under stirring The NaHTe solution prepared in the first step was added to the reaction mixture under N protection The resultant mixture was stirred for ... and then boiled at 100°C The reflux time to get the CdTe QDs was h X- Ray diffraction (XRD) was used to confirm the crystalline phase of QDs Four milliliter of CdTe QDs, approximately 100 μg of ... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value Detection of...

Ngày tải lên: 21/06/2014, 01:20

... self-evident that the area of the convex hull for the ROC with reject option is greater than that without reject option The area of the convex hull is correlated with the area under the ROC that is ... probability of detection approximately 73% for probability of false alarm 4% [33] For the same probability of false alarm, a probability of detection, which falls between 80.95% for rectangular ... likelihood and the fraction in the right-hand side of (1) is called the threshold value of the likelihood ratio for decision [43] Frequently, the decision is expressed in terms of the minus log-likelihood...

Ngày tải lên: 21/06/2014, 20:20

... selectivity when they are used just for Zn(II) detection On the other hand, the fluorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... intracellular environment for the detection of Zn(II) References ð1Þ Wherein, A is the value of the fluorescence intensity, C is the concentration of Zn(II), and the range of C is from 0.2 lM to ... Thus Zn(II) considerably enhances the fluorescence of MWNTs-GNQ To further characterize the performance of the sensing material for Zn(II), a series of comparison experiments were carried out with...

Ngày tải lên: 22/06/2014, 01:20

... , N y the position along image’s y-axis All maxima xi∗ j,max are estimated with a high accuracy in x- direction One major problem concerning the detection of the maxima is that most of the brightest ... difj,max ferent methods: the center -of- mass and the Blais-and-Rioux operators The first uses the distribution of the grey level to retrieve the positions of the maxima, whereas the second applies a ... j,max ∈ R and intensity values fi j,max ∈ Z Figure 11 shows the computing principle for image function f (x, y) ∈ Z64×64 of shape s∗ i j,max and intensity fi j,max values of maxima xi∗ j,max for...

Ngày tải lên: 22/06/2014, 19:20

... at 7x- 4 0x magnification Scrapings of the body surface were done with a cover slip to remove epithelial cells and mucus with parasites for examination in the compound microscope (4 0x- 100 0x) The ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the...

Ngày tải lên: 06/08/2014, 19:20

... recommended for diagnosis of CHV in formalin-fixed tissues The optimized ISH assay, which developed in this study, may be a valid tool for the study of pathogenesis and diagnosis of CHV infection ... digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled probe, which was optimized ... hybridization for CHV In this study, several ISH protocols, which were consisted of the changes of enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed...

Ngày tải lên: 07/08/2014, 17:22

... Australia) The reaction mixture for each one tube TaqMan reaction mix consisted of µl of Universal 5X buffer, µl of 10 mM dNTP, µl of enzyme mix (Qiagen, Germany), µl of 20 pM forward primer, µl of 20 ... value on the y-axis and cycle number on the x- axis The threshold cycle (Ct) was defined as the cycle number at which the reporter fluorescence generated by cleavage of the probe passed a fixed threshold ... assess the correct specificity of the real-time RT-PCR assay Both Anyang 300 and 347 Fig Comparison of real-time RT-PCR and gel electrophoresis analysis of the product for the detection of JEV...

Ngày tải lên: 07/08/2014, 18:20