... Control 58 ± 18 888 ± 30 IL -1 280 ± 29 33 91 ± 98 IL -1 275 ± 22 4055 ± 22 NF1 61 10 -4 M 295 ± 31 3508 ± 82 NF1 61 10 -4 M 288 ± 17 3990 ± 38 NF1 61 10 -5 M 285 ± 23 3330 ± 49 NF1 61 10 -5 M 277 ... 3885 ± 33 NF1 61 10 -6 M 288 ± 28 3452 ± 74 NF1 61 10 -6 M 280 ± 22 3905 ± 55 NF177 10 -4 M 260 ± 21 3660 ± 84 NF177 10 -4 M 268 ± 17 3875 ± 42 NF177 10 -5 M 277 ± 15 3280 ± 94 NF177 10 -5 M 256 ... for 20 h with IL -1 plus either NF177 or NF1 61 (10 -6 10 -4 M). In a second set of experiments, HUVEC were pre-treated with IL -1 for 20 h and then with NF177 or NF1 61 (10 -6 10 -4 M) for a further...
Ngày tải lên: 11/08/2014, 08:21
Ngày tải lên: 11/08/2014, 15:22
Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... & Laursen RA (19 81) Immuno- chemical characterization of the kringle 4 fragment of human plasminogen. J Biol Chem 256, 11 166 11 1 71. 46 Cummings HS & Castellino FJ (19 85) A monoclonal antibody ... to the study of the activation pathways of plasminogen in vivo. J Biol Chem 260, 12 106 12 111 . 57 Wiman B & Wallen P (19 73) Activation of human plas- minogen by an insoluble derivative of urokinase. ... F-75877-Cdx, Paris 18 , France Fax: +33 1 40 25 86 10 Tel: +33 1 40 25 86 11 E-mail: angles@infobiogen.fr (Received 30 March 2005, revised 5 May 2005, accepted 9 May 2005) doi :10 .11 11/ j .17 42-4658.2005.04754.x Plasmin(ogen)...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
... without dots n = 3 Ki -1/ 57 Ki -1/ 57 (1 222) Ki -1/ 57 (1 413 ) B A L CDEF GH I JK Ki -1/ 57 (1 222) Ki -1/ 57 (12 2– 413 ) Ki -1/ 57 (15 1–263) Ki -1/ 57(264– 413 ) Fig. 8. The localization of Ki -1 ⁄ 57 to nuclear bodies ... Scolfaro 10 .000, C.P. 619 2, 13 084-9 71 Campinas, SP, Brasil Fax: +55 19 3 512 10 06 Tel: +55 19 3 512 11 25 E-mail: jkobarg@lnls.br (Received 12 April 2009, revised 8 May 2009, accepted 13 May 2009) doi :10 .11 11/ j .17 42-4658.2009.07092.x The ... GST–hnRNPQ (1 443), GST–Ki -1 ⁄ 57 and the trun- cated constructs Ki -1 ⁄ 57 (12 2– 413 ), Ki -1 ⁄ 57 (15 1–260) and Ki -1 ⁄ 57(2 61 413 ) fused to 6· His were expressed in Escherichia coli BL 21- CodonPlus-RIL...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
... D11A, G13A, S15A, T17A, P19A, C22A, P37A, C38A, M43A, K47A, K47D, K47R, G49A, K50A, K50D, K50I, K50R, H51A, G52A, K55A, F64A, P74A, H77A, M79A, P82A, I84A, R86A, L91A, L101A, P 115 A, E 116 A, L 119 A, ... (K47A), 11 3.8 ± 10 .8 (K47R), 238.7 ± 11 .3 (H51A), 14 4.5 ± 14 .2 (P74A), 15 0 .1 ± 12 .5 (L91A) and 444.0 ± 32.6 min (L101A), whereas that for the strain expressing wild-type heIF5A was 11 2.3 ± 6.8 ... (pBM–TIF51A) (p 414 GAL1–heIF5A-1m) This work HHY 212 S MATa leu2 his3 ura3 trp1 can1 tif51A:: LEU2 tif51B::HIS3 (p 414 GAL1– heIF5A-1m) This work UBHY-R 212 d MATa leu2 his3 ura3 trp1 can1 tif51A:: LEU2 tif51B::HIS3...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme pot
... HO -1. Gly130, Gly134, Gly135, and Asp1 31 correspond to Gly139, Gly143, Gly144, and Asp140 in human and rat HO-1s and to Gly135, Gly139, Gly140, and Asp136 in HmuO. F-helix confomation Gly130 ... accession code 1WE1. (Received 1 1 August 2 004, revised 30 September 2004, accepted 4 October 2004) Eur. J. Biochem. 2 71, 4 517 –4525 (2004) Ó FEBS 2004 doi :10 .11 11/ j .14 32 -10 33.2004.04 411 .x helix ... O–Fe Gly134 N–Fe Gly130 O–Gly134 N Gly130 O–Gly135 N Asp1 31 O–Gly135 N Syn HO -1 closed 4.6–4.9 5 .1 5.4 2.8–3.0 4.0–4 .1 2.9–3.0 Human HO -1 closed 4.8 5.3 3.2 4.3 3 .1 Human HO -1 open 4.9 6.0 3 .1 4.8...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... anti-core +1 pHPI -15 07 anti-core . . . . 14 20 24 Control pHPI -14 95 pHPI -14 94 pHPI -15 07 kDa MG132 1 2 3 4 7 17 1 2 3 4 5 6 7 anti-core +1 Control pHPI -14 96 pHPI -14 96 +MG132 +MG132 +MG132 pHPI -15 79 pHPI -15 79 pHPI -15 80 pHPI -15 80 (a) (b) (a) (b) ... gcccctctATG 85 g nt 515 nt 825nt ntnt nt core +1 myc ( +1) nt nt pHPI -14 96 core +1/ S–myc 1 2 3 4 anti-core +1 7 17 Control pHPI -14 95 pHPI -14 94 pHPI -15 07 kDa MG132 DMSO Lactacystin MG132 1 2 3 anti-core +1 pHPI -15 07 anti-core . . . . 14 20 24 Control pHPI -14 95 pHPI -14 94 pHPI -15 07 kDa MG132 1 ... nt core +1 myc( +1) nt nt del. core nts 342- 514 pHPI -14 95 nt 515 nt 825nt ntnt nt core +1 myc( +1) nt nt del. core nts 342- 514 pHPI -14 95 pHPI -15 80 CMVCMV nt 590 nt 828nt ntnt nt core +1 nt nt core +1/ S optimal...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo khoa học: Solution structure of an M-1 conotoxin with a novel disulfide linkage pdf
... 3.53 Gly7 8 .18 4.58, 3. 31 Cys8 8.42 4.7 3.06 His9 7.3 4.89 3.53, 3.26 d: 7.29 e: 8.67 Glu10 8.92 4 .15 2 .13 c: 2.55 Leu 11 8.45 4.08 2. 01, 1. 72 c: 1. 61 d: 0.94 ± 0.89 Cys12 7.57 4.46 3.22, 3 .17 Tyr13 9.08 ... Number Intraresidual 12 2 Sequential (|I ) j | ẳ 1) 48 Medium range 7 Long range 13 AMBER energies, kcalặmol )1 Bond 2.822 ± 0 .13 6 Angle 34. 311 ± 1. 454 Dihedral 36 .11 4 ± 2.807 VDW )55.829 ± 1. 478 EEL )442.969 ± 13 .886 H-bond ... Chinese Academy of Sciences, 320 YueYang Road, Shanghai 2000 31, China Fax: +86 21 5492 10 11 Tel: +86 21 5492 11 65 E-mail: chi@sunm.shcnc.ac.cn *These authors contributed equally to this study (Received 10 ...
Ngày tải lên: 30/03/2014, 09:20
Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... M. (19 94) The Vpr protein of human immunodeficiency virus type 1 influences nuclear locali- zation of viral nucleic acids in nondividing host cells. Proc. Natl Acad.Sci.USA 91, 7 311 –7 315 . 11 . Nie, ... type 1 forms cation-selective channels in planar lipid bilayers. Proc. Natl Acad.Sci.USA93, 11 1 11 5. Ó FEBS 2002 NMR Structure of the HIV -1 protein Vpr (Eur. J. Biochem. 269) 3787 (Val57, Leu 61, ... a semipreparative Vydac C 18 column using a linear gradient of acetonitrile. An experimental mass of 11 4 31. 92 Da was obtained by electrospray mass spectroscopy and a mass of 11 433.02 Da was calculated...
Ngày tải lên: 31/03/2014, 23:20
Báo cáo sinh học: "The impact of Metastasis Suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance" doc
... tumour suppresor -1, MTSS -1 and the aggressiveness of prostate cancer cells. Exp Therapeut Med 2 011 , 2 :15 7 -16 2. doi :10 .11 86 /14 79-5876-9-95 Cite this article as: Xie et al.: The impact of Metastasis ... 87 Female 18 Age > 55 64 ≤ 55 41 T status 1 1 225 356 419 N status 0 48 1/ 2 53 M status 0 99 12 WHO grade 1 7 246 3 41 47 Xie et al . Journal of Translational Medicine 2 011 , 9:95 http://www.translational-medicine.com/content/9 /1/ 95 Page ... tumours (48 .19 ± 16 .07) (p = 0.024). Xie et al . Journal of Translational Medicine 2 011 , 9:95 http://www.translational-medicine.com/content/9 /1/ 95 Page 4 of 10 RESEARCH Open Access The impact of Metastasis...
Ngày tải lên: 18/06/2014, 19:20
o cáo hóa học:" The impact of Metastasis Suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance" docx
... tumour suppresor -1, MTSS -1 and the aggressiveness of prostate cancer cells. Exp Therapeut Med 2 011 , 2 :15 7 -16 2. doi :10 .11 86 /14 79-5876-9-95 Cite this article as: Xie et al.: The impact of Metastasis ... 87 Female 18 Age > 55 64 ≤ 55 41 T status 1 1 225 356 419 N status 0 48 1/ 2 53 M status 0 99 12 WHO grade 1 7 246 3 41 47 Xie et al . Journal of Translational Medicine 2 011 , 9:95 http://www.translational-medicine.com/content/9 /1/ 95 Page ... tumours (48 .19 ± 16 .07) (p = 0.024). Xie et al . Journal of Translational Medicine 2 011 , 9:95 http://www.translational-medicine.com/content/9 /1/ 95 Page 4 of 10 significantly reduced rate of growth...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo toán học: "Asymptotic Enumeration of Dense 0-1 Matrices with Equal Row Sums and Equal Column Sums" docx
... T 1 2 (S), are these: Q = | j |n 1/ 2+ ,j =1, ,m1 | k |m 1/ 2+ ,k =1, ,n1 |à|(mn) 1/ 2+2 M = |à 1 |m 1/ 2 n 1/ 2+ | 1 |n 1/ 2 m 1/ 2+ B = (1 m 1/ 4 ) 2 à 2 (1 + m 1/ 4 ) 2 (1 n 1/ 4 ) 2 ... of 1 2 Q E 2 and O(H 1 )exp 1 2 + 1 8 A 1 (1 2) 2 + 1 16 A 1 (1 6 +6 2 ) m n + n m ì Amn 1/ 2 An (m1)/2 Am (n1)/2 is exp 1 2 1 2A 24A m n + n m 1+ O(n 1/ 4+/24+4+o (1) )+O(n 1/ 2+/3 +15 /2+2 2 ) ì Amn 1/ 2 An (m1)/2 Am (n1)/2 , which ... abbreviations c 2 = 1 m 1/ 2 (m 1/ 2 +1) 2 ,c 3 = m 1/ 2 +3 m(m 1/ 2 +1) 3 , d 2 = 1 n 1/ 2 (n 1/ 2 +1) 2 ,d 3 = n 1/ 2 +3 n(n 1/ 2 +1) 3 . The determinant of the matrix T 2 is (mn) 1/ 2 ,andso J(m, n)=(mn) 1/ 2 T 1 2 (S) E 1 , where E 1 =exp(L 1 ), and L 1 =...
Ngày tải lên: 07/08/2014, 13:21
Báo cáo khoa học: " Sequence Analysis of Canine LINE-1 Elements and p53 Gene in Canine Transmissible Venereal Tumor" doc
... Acad. S ci. U.S .A. 19 97, 94 , 10 155 -10 160. 14 . Levine, A. J., Momand, J. and Finlay, C. A. The p53 tumour supressor gene. Nature 19 91, 3 51 , 453-456. 15 . McClure, M. A. Evolution of retroposons by ... originate from the 3 region of canine LINE -1. Katzir et al. [11 , 12 ] reported that the 1, 314 -bp LINE -1- like element flanked by 10 -bp direct repeats integrate into the c-myc region of TVT, which is 64-bp smaller ... Rev. Biochem. 19 82, 51 , 813 -844. 10 . Johnson, A. S., Couto, C. G., Weghorst, C. M. Mutation of the p53 tumor suppressor gene in spontaneously occurring osteosarcomas of the dog. Carcinogenesis 19 98, 19 (1) ,...
Ngày tải lên: 07/08/2014, 15:20
Báo cáo khoa học: "Altered expression of thioredoxin reductase-1 in dysplastic bile ducts and cholangiocarcinoma in a hamster model" pptx
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Ngày tải lên: 07/08/2014, 18:21
Báo cáo y học: "Transcriptional regulation of collagenase (MMP-1, MMP-13) genes in arthritis: integration of complex signaling pathways for the recruitment of gene-specific transcription factor" ppt
... appears in EMBO J 19 97, 16 :440]. Embo J 19 93, 12 :3893-39 01. 37. Benbow U, Brinckerhoff CE: The AP -1 site and MMP gene reg- ulation: what is all the fuss about? Matrix Biol 19 97, 15 : 519 - 526. 38. Chamberlain ... fibroblasts. Arthritis Rheum 19 98, 41: 1987 -19 94. 31. Xiao G, Harhaj EW, Sun SC: NF-kappaB-inducing kinase regu- lates the processing of NF-kappaB2 p100. Mol Cell 20 01, 7: 4 01- 409. 32. Heissmeyer V, ... Oncogene 19 95, 10 : 12 09 -12 16. 25. O’Hagan RC, Tozer RG, Symons M, McCormick F, Hassell JA: The activity of the Ets transcription factor PEA3 is regulated by two distinct MAPK cascades. Oncogene 19 96,...
Ngày tải lên: 09/08/2014, 03:24
Báo cáo y học: "Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia" potx
... 11 .79 Aneurysm 18 15 6.64 ± 9 .16 Myocardial ischemia 13 18 0.94 ± 15 .49 Healthy individuals 15 17 1.37 ± 11 .39 Total 95 16 3.59 ± 4.58 MMP-3 Acute aortic dissection 31 12.92 ± 1. 01 Chronic aortic dissection 18 ... C (n = 18 ) Group D (n = 13 ) Group E (n = 15 ) Age mean ± SD 60 .1 ± 14 .8 60.6 ± 13 .1 60.7 ± 13 .7 65.4 ± 10 .3 40 .1 ± 5.2 Male/Female 26/8 17 /1 14/4 13 /0 3 /12 Arterial hypertension (%) 22 (64.7%) 13 ... 3 .16 ± 0. 51 Myocardial ischemia 13 4.58 ± 1. 04 Healthy individuals 15 1. 10 ± 0.38 Total 95 2.95 ± 0.34 MMP-2 Acute aortic dissection 31 1 51. 62 ± 6.82 Chronic aortic dissection 18 17 2 .12 ± 11 .79 Aneurysm...
Ngày tải lên: 10/08/2014, 10:20
Báo cáo y học: " Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy" doc
Ngày tải lên: 12/08/2014, 23:22