cancer stem cells identification and isolation

... Stem Cells to Overcome Drug Resistance 401 Miaorong She and Xilin Chen Chapter 21 Cancer Stem Cells and Chemoresistance Suebwong Chuthapisith Chapter 22 Cancer Stem Cells in Drug Resistance and ... Chapter Prostate and Colon Cancer Stem Cells as a Target for Anti -Cancer Drug Development Galina Botchkina and Iwao Ojima Niches and Vascularization 135 155 Importance of Stromal Stem Cells in Prostate ... Cruz and Isidro Sanchez-Garcia Part Stem Cells in Specific Tumors 61 Chapter Breast Cancer Stem Cells 63 Marco A Velasco-Velázquez, Xuanmao Jiao and Richard G Pestell Chapter Glioma Stem Cells: ...

Ngày tải lên: 12/08/2014, 06:20

... normal stem cells to cancer stem cells Adult normal stem cells represent one purported origin of cancer stem cells What causes the transformation from normal stem cells to cancer stem cells? ... checkpoint activation and apoptosis in cancer stem cells Another possible reason for increased genomic instability in cancer stem cells is malignant transformation of aged stem cells Stem cells have a ... Clarke, M.F., and Weissman, I.L (2001) Stem cells, cancer, and cancer stem cells Nature 414, 105-111 Ringrose, L., and Paro, R (2004) Epigenetic regulation of cellular memory by the Polycomb and Trithorax...

Ngày tải lên: 12/08/2014, 06:20

... [62] Murine and human hematopoietic stem cells [63-64], murine neural stem cells [65], normal and malignant human mammary stem cells [66], and normal and malignant human colorectal stem cells [62,67] ... normal neural stem cells and glioma stem cells, and may identify new CD133- glioma stem cells 4.4 New markers: Podoplanin and Integrin Alpha There are two new promising cancer stem cell markers ... factors For instance, Wnt ligands are produced and released from both stem cells and niche cells, BMP and Shh are released from niche cells and epithelial cells respectively, and Notch signaling is...

Ngày tải lên: 12/08/2014, 06:20

... 118 Cancer Stem Cells Theories and Practice Fig Expression of ALDH1, ALDH2 and CD133 mRNA in immortalized human airway cell and lung cancer cell lines Levels of mRNA of ALDH1, ALDH2 and CD133 and ... chemiluminescence system (ECL, Amersham) This is trial version www.adultpdf.com 124 Cancer Stem Cells Theories and Practice This is trial version www.adultpdf.com Cancer Stem Cells in Lung Cancer: Distinct ... 126 Cancer Stem Cells Theories and Practice Table Cancer stem cell markers in small cell lung carcinoma amd non-small cell lung carcinoma This is trial version www.adultpdf.com Cancer Stem Cells...

Ngày tải lên: 12/08/2014, 06:20

... in stem cell regulation and cancer formation and progression are an attractive area of research Self-renewal of cancer stem cells Stem cells are defined by their multi-lineage differentiation and ... (Papagiannakopoulos and Kosik, 2008) According to this cancer stem cell hypothesis, cancer stem cells are tumorinitiating cells that proliferate uniquely through self-renewal Cancer stem cells are thought ... distinction between normal adult stem cells and cancer stem cells Pharmacology & therapeutics 119, 74-82 This is trial version www.adultpdf.com 16 MicroRNAs and Cancer Stem Cells in Medulloblastoma Massimo...

Ngày tải lên: 12/08/2014, 06:20

... review this issue with depth Stem cells and cancer stem cell theory Stem Cells In order to understand cancer stem cells a brief description of normal adult stem cells and the environment in which ... metastasis, and immunosurveillance avoidance in tumors This is trial version www.adultpdf.com Cancer Stem Cells as a New Opportunity for Therapeutic Intervention 381 Cancer Stem Cells Cancer Stem Cells ... lung cancer Later, In 2008, Chen et al isolated CD133+ and - cancer cell populations from lung cancer cell lines and non-small cell lung cancer patients and reported that CD133+ lung cancer cells...

Ngày tải lên: 12/08/2014, 06:20

... inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human leukemia ... maintained in standard culture (control group) All cells were monitored × 12 h to assess cleavage, differentiation, and attachment to the feeder cell monolayer, as applicable (Figure and 2) With ... on to fresh feeder cells for confirmation and further analysis; no cells were frozen This work carries forward a theoretical approach suggested more than a decade ago [12], and demonstrates that...

Ngày tải lên: 13/08/2014, 23:20

... data analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments ... primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells ... interactions and stem cell self-renewal Results Identification of gene products associated with a cancer stem cell phenotype By comparing RNA expression patterns from stem and committed cells, independent...

Ngày tải lên: 14/08/2014, 08:21

... Burg, and Andrew Baird 393 29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors Jane E Aubin, Fina Liu, and G Antonio Candeliere 403 30 Nonradioactive Labeling and Detection ... Embryonic Stem Cells 1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L Roach and John D McNeish Introduction Embryonic stem (ES) cells were rst isolated in the 1980s ... (ES cells to inject and cells to pellet for DNA) into the microfuge and spin for at 110g To make sure trypsin is removed, aspirate the supernatant and add mL M2 to the cells for injection and...

Ngày tải lên: 08/04/2014, 12:51

... Zheng, and Dennis A Steindler 15 Isolation of Stem and Precursor Cells from Fetal Tissue Yuan Y Wu, Tahmina Mujtaba, and Mahendra S Rao 29 Olfactory Ensheathing Cells: Isolation and Culture ... Alvarez-Buylla, A and Lois, C (1995) Neuronal stem cells in the brain of adult vertebrates Stem Cells 13, 263–272 Production and Analysis of Neurospheres 27 Gage, F., Ray, J and Fisher, L (1995) Isolation, ... neural stem cells in culture The third section, “Utilization/Characterization of Neural Stem Cells in vivo,” is a collection of techniques to identify and characterize endogenous stem cells as...

Ngày tải lên: 11/04/2014, 09:58

... of stem cells in repair of liver injuries Several types of stem cells, such as embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, haematopoietic stem cells, and mesenchymal stem cells, ... Liver Stem Cells Yock Young Dan Identification and Isolation of Adult Liver Stem/ Progenitor Cells Minoru Tanaka and Atsushi Miyajima Isolation and Purification ... liver stem/ progenitor cells exist in normal adult liver Recently, we and others have successfully identied oval cells and adult liver stem/ progenitor cells Here, we describe the identication and isolation...

Ngày tải lên: 29/05/2014, 17:06

... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... distinguish precancerous stem cells from primary cancer stem cells First is the location Precancerous stem cells mainly exist in precancerous lesions, but primary cancer stem cells exist in primary cancer ... primary cancer stem cells initiate from; whether primary cancer stem cells are same in the same type of cancer among different patients; and how to distinguish cancer stem cells from normal stem cells...

Ngày tải lên: 18/06/2014, 19:20

... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... distinguish precancerous stem cells from primary cancer stem cells First is the location Precancerous stem cells mainly exist in precancerous lesions, but primary cancer stem cells exist in primary cancer ... primary cancer stem cells initiate from; whether primary cancer stem cells are same in the same type of cancer among different patients; and how to distinguish cancer stem cells from normal stem cells...

Ngày tải lên: 20/06/2014, 03:20

... against cancer stem cells discriminate between cancer stem cells and normal stem cells This will require the identification of realistic drug targets unique to cancer stem cells The identification ... normal stem cells and cancer stem cells, there are mechanistic differences in some cancers Interestingly, the mechanistic differences in selfrenewal between normal stem cells and cancer stem cells ... multi-potential stem cells, tissue-specific stem cells, progenitor cells, mature cells, and cancer cells described the direct isolation of populations containing cancer stem cells in various...

Ngày tải lên: 20/06/2014, 07:20

... stem cells These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... tremendous variety of approaches and techniques, and this book, Embryonic Stem Cells - Differentiation and Pluripotent Alternatives and its companion, Embryonic Stem Cells - Basic Biology to Bioengineering, ... Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine 451 Tammy Laberge and Herman S Cheung Chapter 24 Pluripotent Stem Cells from Testis Sandeep Goel and Hiroshi...

Ngày tải lên: 27/06/2014, 19:20

... stem cells These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... tremendous variety of approaches and techniques, and this book, Embryonic Stem Cells - Differentiation and Pluripotent Alternatives and its companion, Embryonic Stem Cells - Basic Biology to Bioengineering, ... Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine 451 Tammy Laberge and Herman S Cheung Chapter 24 Pluripotent Stem Cells from Testis Sandeep Goel and Hiroshi...

Ngày tải lên: 28/06/2014, 04:20

... Mouse and human expression of shared molecules between inflammation and stem cells Mouse and human expression of shared molecules between inflammation and stem cells Comparison of mouse and human ... and2 inflammatory cells Inflammatory receptors and mediators shared between stem Inflammatory receptors and mediators shared between stem cells and inflammatory cells List of inflammatory receptors and mediators ... trafficking and 3) inflammatory cytokines produced by hematopoietic stem cells homing and retention in the bone marrow niche [18,19] Likewise, c-kit, the receptor for stem cell factor (SCF) and a known...

Ngày tải lên: 08/08/2014, 21:20

... overexpression P63+ basal /stem cells were transformed into AR+ or ARcancer cells but rarely into ChgA+ neuroendocrine cancer cells Conventional human prostate cancer cells include malignant cells of luminal, ... Cancer 2006, 42:1213-1218 Lawson DA, Witte ON: Stem cells in prostate cancer initiation and progression J Clin Invest 2007, 117:2044-2050 Lang SH, Frame FM, Collins AT: Prostate cancer stem cells ... co-expressed with P63+ in some cancer cells of the advanced prostate cancer (arrow-indicated respectively in (c) and (f)) (C) P63+ cancer cells recapitulated prostate-like glandular structure formation...

Ngày tải lên: 10/08/2014, 05:21