calculations of proliferation and estimates of size
ROLE OF THE IMMUNE SYSTEM IN TUMOR PROGRESSION 2
Ngày tải lên :
10/09/2015, 15:50
... Appendix 1: Calculations of proliferation and estimates of size Effect of reduced mitotic index on tumor diameter: At early time points, tumor growth is assumed to be exponential and described ... the number of melanoma cells at the end of the experiment, N is the number of melanoma cells at the onset of the experiment, k is the fraction of proliferating cells, t is the duration of the experiment ... the number of melanoma cells at the end of the experiment, N is the number of melanoma cells at the onset of the experiment, k is the fraction of proliferating cells, t is the duration of the experiment...
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Báo cáo khoa học: Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thaliana docx
Ngày tải lên :
30/03/2014, 15:20
... comparison of the lipid content of AtLCAT3transformed yeast and that of the void plasmid-transformed yeast showed a decrease (by a factor 2) of the PC, PE and PS contents and a strong increase of the ... allowing isolation of positive clones Two of the clones (211 and 314) were selected and sequenced, 314 was shown to be a full-length cDNA of 1691 bp encoding a polypeptide of 451 amino acids ... folding topology related to that of the catalytic domain of animal cytosolic PLA2s Mutagenesis confirmed the critical role of Ser77 and Asp215 in enzymatic activity and of His109 in enzyme stability...
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Báo cáo khoa học: "Aquaporin 1 expression in tissues of canines possessing inherited high K+ erythrocytes" pptx
Ngày tải lên :
07/08/2014, 20:23
... accumulation of three amino acids (Asp, Glu, and Gln) and glutathione [6,11] The volume of HK cells is greater than that of LK cells, the lifetime of the HK cells is half that of LK cells, and some of ... shown (B) AQP1 expression of each tissue was standardized using the signal intensity of GAPDH (C) AQP1 expression was standardized by that of GAPDH in each tissue Standardization revealed abundant ... 4.2 kb band in any HK dog tissue preparation The 1.4 kb band was predominant in skeletal muscle and small intestine of HK dogs, whereas only skeletal muscle exhibited a predominant 1.4 kb band in...
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Báo cáo y học: "Emphysema is associated with increased inflammation in lungs of atherosclerosis-prone mice by cigarette smoke: implications in comorbidities of COPD" ppsx
Ngày tải lên :
11/08/2014, 03:20
... lungs of ApoE-/- mice Figure SIRT1 levels and activity, and eNOS level in lungs of ApoE-/- mice exposed to CS SIRT1 and eNOS levels were measured in lungs of WT and ApoE-/- mice exposed to CS (A and ... increased levels and activities of matrix metalloproteinases, and reduction of SIRT1 levels and activity as well as eNOS levels in lungs by CS MMPs, particularly increased levels of MMP-9 and MMP-12, ... the levels of proinflammatory mediators, such as MCP-1 and KC, which can recruit macrophages and neutrophils in the lung, were measured in lung homogenates of air- and CS-exposed WT and ApoE-/-...
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Báo cáo y học: "Emphysema is associated with increased inflammation in lungs of atherosclerosis-prone mice by cigarette smoke: implications in comorbidities of COPD" potx
Ngày tải lên :
11/08/2014, 06:22
... lungs of ApoE-/- mice Figure SIRT1 levels and activity, and eNOS level in lungs of ApoE-/- mice exposed to CS SIRT1 and eNOS levels were measured in lungs of WT and ApoE-/- mice exposed to CS (A and ... increased levels and activities of matrix metalloproteinases, and reduction of SIRT1 levels and activity as well as eNOS levels in lungs by CS MMPs, particularly increased levels of MMP-9 and MMP-12, ... the levels of proinflammatory mediators, such as MCP-1 and KC, which can recruit macrophages and neutrophils in the lung, were measured in lung homogenates of air- and CS-exposed WT and ApoE-/-...
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Báo cáo y học: "Analysis of gene expression in operons of Streptomyces coelicolor" ppsx
Ngày tải lên :
14/08/2014, 16:21
... enhancement of expression due to presence of the promoter (as expected) As a control, when selecting the same number of genes randomly from all of the documented S coelicolor operons and randomly ... methods These methods often use the presence of promoters and/ or terminators at the start and/ or end of an operon as signals to delineate operon boundaries, and clearly in the cases of S coelicolor ... f g h i Known operon Figure Examples of positive and control sets of operons/non-operons Examples of positive and control sets of operons/non-operons 5) and from the transcriptional unit annotation...
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Báo cáo sinh học: "CGB and GNRH1 expression analysis as a method of tumor cells metastatic spread detection in patients with gynecological malignances" potx
Ngày tải lên :
18/06/2014, 22:20
... expression of CGB (mean of log10 of CGB expression: -2.13) consisted of 36.8% of tissues, while the second with higher CGB activity (mean of log 10 of CGB expression: -1.35) included 63.2% of samples ... the presence of tumor cells circulating in peripheral blood of gynecological cancer patients Thus, the expression of CGB and GNRH1 may become a prognostic factor of metastatic spread of tumor cells ... expression in blood of healthy volunteers and patients with cancer differed significantly (P = 0.001066) and was higher in blood of cancer patients In case of GNRH1 analysis the difference of the gene...
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Báo cáo y học: "Green tea polyphenol epigallocatechin-3-gallate inhibits advanced glycation end product-induced expression of tumor necrosis factor-α and matrix metalloproteinase-13 in human chondrocyte" ppsx
Ngày tải lên :
09/08/2014, 14:20
... clear bands on a background of uniform light blue staining Electrophoretic migration of MMP-13 in the supernatant was compared with known molecular weight standards and also with clear bands of MMP-13 ... Densitometric analysis Measurements of the scanned bands were performed using Un-Scan-It software Each band was scanned five times, and the mean band intensity (pixels per band) was obtained Data were ... for 45 minutes, and then the phosphorylation of p38, JNK, and ERK was determined by western blot analysis (b) Band images were digitally captured and the band intensities (pixels/band) were obtained...
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báo cáo khoa học: " All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein" pdf
Ngày tải lên :
10/08/2014, 10:20
... acids might contribute to treatment of cancers In this work, we studied the effect of ATRA on GIST cells in term of inhibition of cell proliferation, and induction of apoptosis For the first time ... Dimitrijevic S, Nielsen OS: Update of phase I study of imatinib (STI571) in advanced soft tissue sarcomas and gastrointestinal stromal tumors: a report of the EORTC Soft Tissue and Bone Sarcoma Group Eur ... et al Journal of Experimental & Clinical Cancer Research 2010, 29:165 http://www.jeccr.com/content/29/1/165 Page of Figure Effect of ATRA on cell proliferation of GIST-T1, GIST-882 and human normal...
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Báo cáo y học: "Expression of tumor necrosis factor-alpha converting enzyme and matrix metalloproteinase-3 in proliferated synovium in a patient with synovitis-acne-pustulosis-hyperostosis-osteitis syndrome: a case report" doc
Ngày tải lên :
11/08/2014, 14:21
... was no recurrence of synovitis, no exacerbation of palmoplantar pustulosis, and MMP-3 and CRP levels remained normal Discussion We investigated the histopathological features of SAPHO syndrome ... examples of the application of these agents have been limited to recalcitrant cases Further investigations are clearly needed to elucidate this rare and complicated disorder and the expression of TACE, ... KK was the primary physician and orthopedic surgeon, conceived the original study, organized and analyzed the data and prepared the draft of the manuscript HY, NT and MY were consulting orthopedic...
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Expression and editing of MicroRNA 376 cluster in human glioblastomas role in tumor growth and invasion
Ngày tải lên :
10/09/2015, 08:28
... configuration of dsRNA structures undergoing site-specific editing Similarity of structures and location of editing sites in coding sequences of Gabra-3 and GluR-B, and non-coding sequences of pri-miR-376c ... editing status of miR-376 cluster in gliomas and identify candidate miRNAs for characterization of altered A-to-I miRNA editing Analysis of functional consequences of aberrant editing of candidate ... involvement of miRNAs in regulation of developmental pathways and differentiation of cells At least three features of miRNA genomics and expression support a role for miRNAs in cancers: Location of human...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
Ngày tải lên :
12/02/2014, 10:20
... growth and mortality of insect pests, Plutella xylostella (PX) and Spodoptera litura (SL).17th International Congress of Biochemistry and Molecular Biology Annual Meeting of the American Society of ... stop codon and Xho I restriction site was introduced on the end of the proposed synthetic trypsin inhibitor gene The sequences of the forward and reversed primers are shown on fig and of the synthetic ... (bp) 0.1 0.2 0.3 0.4 0.5 0.6 (b) Fig 8: Electrophoresis of the PCR product of MCoTI-II gene on 2% agarose gel (a) and estimation of its size by using SHARP JX-330 scanner (b) 0.7 0.8 Rf 0.9 M:...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
Ngày tải lên :
16/02/2014, 09:20
... causing activation of Bax and Bak [23] Regulation of Bcl-2 family members can occur by a number of mechanisms, including up-regulation of synthesis, enhancement of degradation and phosphorylation ... level of Bcl-2 family genes and the phosphorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members However, each type of cancer has its own way of ... ⁄ and the PI3K ⁄ Akt pathways results, in turn, in the inactivation of pro-apoptotic Bcl-2 proteins (e.g PUMA, Bax, Bim and Bad), Fas, and CDK inhibitors (e.g p27KIP1, p21WAF1 and p15INK4b), and...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
Ngày tải lên :
18/02/2014, 18:20
... the presence of cytokines Natural ligands of ICAM- 1and LFA-1 are expressed on the surface of T and B lymphocytes, natural killer cells, monocytes, macrophages and granulocytes [33], and interaction ... activity of ERK, p38 and SAPK ⁄ JNK, the three major members of the MAPK family, in LPS-treated SCs Briefly, as illustrated in Fig 5A, phosphorylation of p38 and SAPK ⁄ JNK appeared at 30 min, and ... phosphorylated ERK, p38 and JNK (p-ERK, p-p38 and p-JNK) and total ERK, p38 and JNK (tERK, tp38 and tJNK) (B) The ratio of phosphorylated to total ERK (p44 ⁄ 42), p38 and JNK at each time point The data...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
Ngày tải lên :
19/02/2014, 16:20
... ÔStainsAllÕ analysis of Fig and the 1D immunoblotting of Fig As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of a-dystroglycan ... listed in Table The pH values of the first dimension gel system and molecular mass standards (in kDa) of the second dimension are indicated at the top and on the left of the panels, respectively ... pattern and often result in a streaky spot pattern Therefore, silverstained 2D patterns of muscle proteins probably overestimate the presence of soluble proteins and underestimate the expression of...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
Ngày tải lên :
19/02/2014, 16:20
... Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation This article describes the differing effects of n-3 and n-6 PUFAs on the proliferation of SMC ... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... Modulation of smooth muscle cell (SMC) proliferation and retinoblastoma protein (Rb) phosphorylation after supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) (A,B) Effects of n-3 and...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
Ngày tải lên :
21/02/2014, 15:20
... recombination [27] of two plasmids, one containing the right hand portion of the adenoviral vector genome and a lox site, and the other plasmid, pAvhUCP3lx, containing the left portion of the viral ... the oligomycin-resistant portion of respiration reflects the level of proton leak of mitochondria, the low level of respiration in the presence of oligomycin of L6 cells showed that they were ... muscles of KO mice was found to be much more efficient and the rate of ATP production markedly increased [22] Therefore, although the lack of UCP3 has strong effects on the efficiency of oxidative...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
Ngày tải lên :
06/03/2014, 19:20
... representative TEM images of mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the ... The number and area of mitochondria in the eWAT and iBAT A-B Representative TEM images of eWAT C-D Representative TEM images of iBAT (Arrows point to mitochondria) E: The analysis of mitochondrial ... on our understanding of the adverse effects of particulate air pollution on cardiometabolic diseases, especially in the context of obesity and insulin resistance Xu et al Particle and Fibre Toxicology...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
Ngày tải lên :
07/03/2014, 17:20
... date, four isoforms of PDK (PDK1–4) and two isoforms of PDP (PDP1–2) have been identified in humans [3–5] These isoforms display unique tissue distribution [3–5] and varied kinetic and regulatory ... Regulation of hPDK2 and hPDK4 gene expression Table The effects of PPAR a, d and c agonists on PDK2 and PDK4 transcript abundance expressed as a percentage of basal levels Results are the means ± SEM of ... 50 pmol of each gene-specific primer, ng of double-stranded cDNA, dNTPs (200 lm), buffers and 0.5 U of Expand High Fidelity Polymerase (Roche Diagnostics Ltd, Lewes, UK) in a final volume of 100...
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