calcium calmodulin dependent protein kinase ii in cardiac disease and arrhythmia
Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt
Ngày tải lên :
16/03/2014, 16:20
... Phospholipase C, protein kinase C, Ca2+ /calmodulin- dependent protein kinase II, and tyrosine phosphorylation are involved in carbachol-induced phospholipase D activation in Chinese hamster ovary ... activation is dependent on Ca2+ and possibly involves the Ca2+-activated protein kinase, CaM kinase II (Fig 9D) These data suggest that EGCG activates PKC-a in U87 cells Involvement of PKC in EGCG-induced ... involving redox -dependent changes in the cell, which stimulate the PLC-c1 [Ins(1,4,5)P3–Ca2+]–Ca2+ /calmodulin- dependent protein kinase II (CaM kinase II) –PLD pathway and the PLC-c1 (diacylglycerol)–PKC–PLD...
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Báo cáo Y học: Autophosphorylation-dependent inactivation of plant chimeric calcium/calmodulin-dependent protein kinase docx
Ngày tải lên :
17/03/2014, 17:20
... of plant chimeric calcium/ calmodulin- dependent protein kinase J Biol Chem 276, 32940–32947 13 Colbran, R.J & Soderling, T.R (1990) Calcium/ calmodulindependent protein kinase II Curr Top Cell Regul ... Ischemiainduced translocation of Ca2+ /calmodulin- dependent protein Ó FEBS 2002 Chimeric calcium/ calmodulin dependent protein kinase (Eur J Biochem 269) 2463 kinase II: potential role in neuronal ... Poovaiah, B.W (2000) Plant chimeric Ca2+ /Calmodulin- dependent protein kinase Role of the neural visinin-like domain in regulating autophosphorylation and calmodulin affinity J Biol Chem 275, 30417–30422...
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báo cáo khoa học: " A rice calcium-dependent protein kinase is expressed in cortical root cells during the presymbiotic phase of the arbuscular mycorrhizal symbiosis" pps
Ngày tải lên :
11/08/2014, 11:20
... and calmodulin- dependent protein kinase (CCaMK in L japonicus; DMI3 in M truncatula) and CYCLOPS (LjCYCLOPS; DIM3-interacting protein in M truncatula) [16-21] CCaMK interacts with, and phosphorylates, ... Infections 2” in M truncatula), two nucleoporins (NUP85 and NUP133), two cation channel proteins (the L japonicus CASTOR and POLLUX proteins; DMI1a and DMI1b in M truncatula), a calcium and calmodulin- dependent ... of protein kinases and represent a differentiated group of protein kinases found in plants, algae and protists [34-36] They possess a characteristic structure consisting of four domains: an amino...
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Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx
Ngày tải lên :
07/03/2014, 06:20
... assayed for protein kinase activity Three distinct peaks associated with protein kinase activity were separated: I, II and III Aliquots of fractions were also analysed by (B) Coomassie-stained SDS ... human Ca-isoform contained only a 40 kDa protein, whereas those corresponding to peak II contained two different protein bands of 41 and 43 kDa (Fig 2B) The three protein bands showed reactivity ... eliminate most contaminant proteins, and C-subunit isoforms were eluted by applying a continuous NaCl gradient (0–0.4 m in buffer C) The collected fractions of 0.5 mL were assayed for protein kinase...
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Báo cáo khoa học: The hinge region operates as a stability switch in cGMP-dependent protein kinase Ia doc
Ngày tải lên :
07/03/2014, 09:20
... enzyme from the AGC-family of protein kinases, cAMP -dependent protein kinase (PKA) have greatly contributed to our understanding of PKG’s intra- and inter-domain interactions, particularly the ... sites in cAMP -dependent protein kinase are transposed in type Ibeta cGMP -dependent protein kinase J Biol Chem 271, 17570–17575 15 Corbin JD & Doskeland SO (1983) Studies of two different intrachain ... (2000) Rising behind NO: cGMP -dependent protein kinase J Cell Sci 113, 1671–1676 Vo NK, Gettemy JM & Coghlan VM (1998) Identification of cGMP -dependent protein kinase anchoring proteins (GKAPs)...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
Ngày tải lên :
16/03/2014, 12:20
... by embryonic kinases and, furthermore, suggest a transient change in protein kinase activity during zebrafish development It is worth mentioning that CNBP phosphorylation began to increase at the ... Blue stained gels Tyr residues and, furthermore, to confirm the data obtained from phospho-site predictor servers CNBP is mainly phosphorylated by cAMP -dependent protein kinase To identify kinases ... of human La protein increases binding to and shifts the 5¢ TOP-mRNA encoding rpL37 off the polysome distribution suggesting that increased levels 492 of nonphosphorylated La protein may have...
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Báo cáo Y học: Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues pptx
Ngày tải lên :
17/03/2014, 23:20
... indeed located in the N-terminal portion of p25rum1 protein within amino acids 3–74 N-Terminal deletion does not in uence the activity of p25rum1 as a Cdc2 kinase inhibitor The p25rum1 protein ... GST–p25rum1-DN74 proteins as a Cdc2 kinase inhibitor (Fig 3B) Therefore, the data indicate that N-terminal phosphorylation of p25rum1 indeed reduces the Cdc2 kinase inhibitor activity of this protein Determination ... (lane 1) and prephosphorylated (lane 2) GST fusion proteins of the DN74 mutant were examined as in (A) (D) Cdc2 kinase activity against Histone H1 was examined by in vitro kinase assay in the presence...
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Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt
Ngày tải lên :
23/03/2014, 13:20
... O.K & Doskeland, S.O (1997) Synergistic antiproliferative actions of cyclic adenosine 3¢,5¢-monophosphate, interleukin-1beta, and activators of Ca2+ /calmodulin- dependent protein kinase in primary ... autoradiography of the wet gel Protein kinase assays Two independent clones of stably expressing HIP/PAP Chang cells (HIP and HIP 4) and two independent control clones (PC4 and PC8) stably transfected ... markers run in parallel with the samples Ó FEBS 2004 Fig Protein kinase activity in HIP/PAP expressing Chang cells Protein kinase activity was assayed with 50 lM kemptide as the substrate in the...
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Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
Ngày tải lên :
18/02/2014, 11:20
... proteins has been not been demonstrated [5,6,17] Data are emerging that point to perilipin as a key player in directing proteins involved in lipolysis to a subset of lipid droplets [5] Interestingly, ... phosphorylation This gain in hydrophobic surface area presumably accounts for the increase in in vitro activity of HSL following PKA phosphorylation through increased binding between HSL and the lipid ... phosphorylation induces a conformational change that increases the accessible hydrophobic surface area, enabling freer access to the lipid-binding site, in return for weaker binding This is well in line...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins are localized in the matrix compartment in a membrane-extrinsic topology ... these proteins are translocated to the mitochondrial compartment remain unclear [35,36] The mitochondrial inner membrane-associated carrier protein, uncoupler proteins and outer membrane proteins ... suggesting that an open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP1A1 requiring N-terminal truncation, we have shown that intact...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
Ngày tải lên :
19/02/2014, 06:20
... Eugene, OR, USA) using bovine serum albumin as a standard Protein identification The N-terminal structures of proteins, including nucleolin, Ku80, nucleolinD, HMGB1 and HMGB2, were determined with an ... DNA -dependent protein kinase: DNA binding and activation in the absence of Ku Proc Natl Acad Sci USA 95, 525–530 32 Hammarsten O, DeFazio LG & Chu G (2000) Activation of DNA -dependent protein kinase ... Mini S PC 3.2 ⁄ column (Amersham Biosciences) in R buffer containing 0.1 m NaCl (Fig S3) Each of the adsorbates, including DNA -dependent protein kinase catalytic subunit (DNA-PKcs), nucleolin,...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
Ngày tải lên :
22/02/2014, 04:20
... of protein kinase inhibitors on the enhancement by TS of LPS/IFN-induced NO production Protein kinases such as PKA and PKC, and various factors such as tumor necrosis factor receptor (TRAF6) and ... of extracellular signal-regulated kinase and c-Jun NH2-terminal kinase but not p38 mitogen-activated protein kinases is required for RRR-a-tocopheryl succinate-induced apoptosis of human breast ... L.-T., Raung, S.-L., Lin, P.-L & Lin, C.-N (1999) Stimulation of respiratory burst by cyclocommunin in rat neutrophils is associated with the increase in cellular Ca2+ and protein kinase C activity...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... mutations to abolish protein phosphorylation was a result of other protein kinases masking the PKA-catalysed part Indeed, protein kinases other than PKA, including 6222 tyrosine kinases, have been ... determinant in their surroundings, a lysine at )3 (S401) and at )2 (T407), with lysine representing a weaker determinant than arginine Accordingly, their contribution to PKA-catalysed in vitro ... from bovine atrium, represented a prominent target for several S ⁄ T protein kinases in vitro, including cAMP-activated PKA [17] To investigate whether GIRK1 represents a target for PKA also in vivo,...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
Ngày tải lên :
07/03/2014, 15:20
... the protein kinase inhibitor of cAMPdependent protein kinase J Biol Chem 272, 18169–18178 Gamm, D.M & Uhler, M.D (1995) Isoform-specific differences in the potencies of murine protein- kinase inhibitors ... of cAMP -dependent protein kinase in mouse J Biol Chem 268, 10927–10931 Olsen, S.R & Uhler, M.D (1991) Inhibition of protein kinase- a by overexpression of the cloned human protein- kinase inhibitor ... an inhibitor protein of cAMP -dependent protein kinase J Biol Chem 266, 11158–11162 Scarpetta, M.A & Uhler, M.D (1993) Evidence for two additional isoforms of the endogenous protein kinase inhibitor...
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Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx
Ngày tải lên :
22/03/2014, 21:21
... the Ca2+-independent ABA-activated SnRK2.6 kinase [21] In addition, the SnRK2.6 kinase could phosphorylate T306 in an in vitro kinase assay This evidence suggests that multiple protein kinases ... two-step PCR using HindIII site-containing sense primer and BamHI site-containing antisense primer (Table S1, see Supporting information) [49] The HindIII-BamHI DNA fragments were ligated into the ... DAG kinases in plants [41], and carrot CDPK, DcCPK1, and maize CDPK, ZmCK11, are activated by PAs and Ca2+ [48,74] The phosphoinositide -dependent protein kinase- 1 specifically binds PA [75] In...
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Báo cáo khoa học: Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester ppt
Ngày tải lên :
23/03/2014, 13:20
... NF-kappaB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF-kappaB-inducing kinase and IkappaB kinase Mol Cell Biol 20, 1278–1290 Ishii T, Kwon H, Hiscott J, Mosialos ... Activation of the IkappaB alpha kinase (IKK) complex by double-stranded RNA-binding defective and catalytic inactive mutants of the interferon-inducible protein kinase PKR Oncogene 20, 1900–1912 ... of in uenza viruses into cells is inhibited by a highly specific protein kinase C inhibitor J Gen Virol 81, 2697–2705 Constantinescu SN, Cernescu CD & Popescu LM (1991) Effects of protein kinase...
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Báo cáo khoa học: Regulation of the muscle-specific AMP-activated protein kinase a2b2c3 complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme docx
Ngày tải lên :
30/03/2014, 08:20
... (1995) 5¢-AMP activates the AMP-activated protein kinase cascade, and Ca2+ ⁄ calmodulin activates the calmodulin- dependent protein kinase I cascade, via three independent mechanisms J Biol Chem 270, ... Momcilovic M, Johnstone SR, Carlson M & Carling D (2005) Ca2+ ⁄ calmodulin- dependent protein kinase kinase-beta acts upstream of AMP-activated protein kinase in mammalian cells Cell Metab 2, 21–33 ... Edelman AM, Frenguelli BG & Hardie DG (2005) Calmodulin- dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase Cell Metab 2, 9–19 49 Sakamoto K, Mccarthy...
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Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc
Ngày tải lên :
30/03/2014, 15:20
... Thr18 and Ser9 Introduction of the single Thr18 and Ser9 mutations in the wild-type protein background resulted in the abrogation of one band, further indicating that these sites are genuine targets ... although insufficient, has been shown to be required for sequenceindependent binding [33] Alternatively, a second possibility is that full-length p53 becomes involved in a protein protein interaction ... target site is in determining the specificity of the kinase DNA-PK is not the only candidate kinase for phosphorylation of p53 at Ser9 and Thr18 Previous work has shown that casein kinase also has...
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Báo cáo khoa học: Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm pdf
Ngày tải lên :
30/03/2014, 15:20
... nuclear and cytoplasmic salt-inducible kinase (SIK1) mutants repress cAMP-response element (CRE)/CRE-binding protein (CREB) activities (A) The protein kinase A (PKA)-induced CRE activity, and its ... SIK1 and Ser587 in SIK2, are indicated in bold The basic residues in domain 3B are indicated by underlines The similarity between SIK1 and SIK2 in domain 3A, the N-terminal half of domain 3, ... for the intracellular distribution and cAMP-response element-binding protein (CREB)-modulating activity of salt-inducible kinase (SIK1) The kinase domain is indicated as KD The region 567–585...
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Báo cáo y học: "Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes" pps
Ngày tải lên :
09/08/2014, 10:21
... glucosamine was ineffective in preventing phosphorylation of this kinase JNK and p38 are involved in cellular stress; they are phosphorylated in response to proinflammatory cytokines, resulting in ... amino-terminal kinase (JNK) and total JNK, (b) p-p38 and total p38, and (c) p-extracellular signal-regulated kinase (ERK)1/2 and total ERK1/2 were used to visualize mitogen-activated protein kinase ... NH2-terminal kinase, p38mitogen-activated protein kinase and activating protein- I J Pharmacol Exp Ther 2003, 305:981-988 40 Ho LJ, Lin LC, Hung LF, Wang SJ, Lee CH, Chang DM, Lai JH, Tai TY: Retinoic...
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