cad ntf1 by calpain inhibition both induces spheroid disadherence and prevents in vitro spheroidgenesis
Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf
Ngày tải lên :
16/03/2014, 01:20
... confirmed by DNA sequencing Immunoblotting Cells were harvested by scraping from culture dishes in hot 1· SDS sample buffer, and the lysates were separated by SDS–PAGE and analyzed by immunoblotting ... confirmed inhibition of protein synthesis by monitoring expression of c-Fos protein, which is a FEBS Journal 276 (2009) 3269–3276 ª 2009 The Authors Journal compilation ª 2009 FEBS 3271 NGF induces ... 100 ngÆmL)1 NGF for h Inhibition of sustained ERK1 ⁄ activation by U0126 was confirmed by immunoblotting (B) The expression levels of miR-221 ⁄ 222 were examined as described in Fig 1C (*P < 0.05,...
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Báo cáo y học: "Glucosamine prevents in vitro collagen degradation in chondrocytes by inhibiting advanced lipoxidation reactions and protein oxidation" pdf
Ngày tải lên :
09/08/2014, 10:20
... relate these in vitro findings to the retardation of cartilage degradation reported in OA trials investigating glucosamine Conclusion In an in vitro model of cartilage collagen degradation in which ... that the decrease in collagen degradation by glucosamine observed in this in vitro model system may be mediated by the inhibition of advanced lipoxidation reaction, preventing the oxidation and ... glucosamine hydrochloride and glucosamine sulfate on collagen degradation As shown in Figure 2, increasing the concentration of both the glucosamine hydrochloride and glucosamine sulfate resulted in...
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Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot
Ngày tải lên :
21/02/2014, 15:20
... preincubation (Fig 4B) In contrast, preincubation with erythromycin resulted in a 20% decrease in splicing inhibition (Fig 4B), con®rming that erythromycin does not inhibit nuclear splicing by ... reduction in splicing inhibition indicates that erythromycin does not inhibit nuclear splicing by interacting with the pre-mRNA Thus, this ®nding is consistent with the idea that erythromycin interacts ... splicing inhibition by Cl-tetracycline may be explained by the additional size and hydrophobicity introduced by the substitution, as is often the case in ribozyme inhibition by tetracyclines...
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Báo cáo khoa học: Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters ppt
Ngày tải lên :
08/03/2014, 02:20
... was examined by sequencing in both directions At this stage, two nonconservative point mutations were found in the first thrombospondin repeat G1851A and A1855G resulting in the following amino acid ... recombinant aggrecanase activities in our enzyme preparations, with no contaminating proteolytic activities being detected Inhibition of ADAMTS activity by catechin gallate esters Work in our ... maximum inhibition observed was still below 50% (EGCG; 29 ± 4% inhibition of collagenase-1 activity and 30 ± 9% inhibition of collagenase-3: ECG; 14 ± 5% inhibition of collagenase-1 and 20 ± 7% inhibition...
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Báo cáo khoa học: Proteolytic degradation of nitric oxide synthase isoforms by calpain is modulated by the expression levels of HSP90 potx
Ngày tải lên :
23/03/2014, 07:20
... This explains why both NOS are inactivated by calpain in the absence of HSP90 The removal of the PDZ domain by calpain, which occurs without loss of catalytic activity in NOS even in the presence ... NOS isozymes by calpain M Averna et al Scheme Binary and ternary complexes generated by HSP90, NOS and calpain CLP, calpain; CST, calpastatin remained associated with HSP90, resulting in the formation ... binding site also at basal Ca2+ levels [7] NO production is highly regulated by many factors and mechanisms, including protein–protein interactions involved in allosteric regulation, scaffolding...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... al In vivo degradation of NOS and HSP90 by calpain higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following ... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... produced by active calpain [32], these observations further indicate that calpain is activated in both tissues, although at a higher rate in aorta Further direct evidence in support of calpain activation...
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Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx
Ngày tải lên :
30/03/2014, 10:20
... the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is required for inactivating the spindle checkpoint signal and progression in the cell cycle [20] The ... The results indicated that the sister kinetochores are not under tension in the presence of benomyl, resulting in the inhibition of degradation of the checkpoint protein BubR1 at the kinetochore ... Benomyl inhibited HeLa cell proliferation by arresting the cells at mitosis and induced cell death (A) Inhibition of proliferation (s) and mitotic progression (m) in HeLa cells by benomyl The inhibition...
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báo cáo hóa học: " Inhibition of the alternative complement activation pathway in traumatic brain injury by a monoclonal anti-factor B antibody: a randomized placebo-controlled study in mice" pot
Ngày tải lên :
19/06/2014, 22:20
... receptor type 1-related protein y, IgG1-linked murine recombinant fusion protein; MBP, mannose-binding protein; rVCP, recombinant Vaccinia virus complement control protein; sCR1, soluble complement ... represents an approach with potential clinical implications In order to induce a continuing complement inhibition during the acute inflammatory phase in the first days, injections were repeated at 24 ... (in 0.1% milk in PBS containing 0.05% TWEEN) and murine recombinant mouse C5a at defined concentrations for establishing the standard curve After incubation and subsequent washing steps, biotinylated...
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Báo cáo hóa học: " The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay" ppt
Ngày tải lên :
20/06/2014, 01:20
... sugar side-chains of the mucins, a purely physical phenomenon [10,24-26], resulting in preventing the virus from entering the host cells (CEM SS cells) This was supported by our finding that salivary ... http://www.virologyj.com/content/5/1/59 Inhibition of HIV-1 activity by crude pregnancy plug mucus and purified pregnancy plug mucins in vitro assay Figure Inhibition of HIV-1 activity by crude pregnancy plug ... MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type in an inhibition assay Virol J 2006, 3:99 Habte HH, de Beer C, Lotz ZE, Tyler MG, Kahn D, Mall AS: Inhibition of Human...
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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
Ngày tải lên :
20/06/2014, 01:20
... powerful tool for the inhibition of virus replication and has a high therapeutic potential In our case, the inhibition effect is not so well-defined as in the result reported by Chen et al [24] ... supported by a grant from the Bingtuan Doctor Foundation Program (05JC04, XinJiang, China) References survival 40% 11.1% survival Competing interests 5LD50 20LD50 FMD3 in this study, siRNAs targeting ... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering...
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Báo cáo hóa học: " In vitro inhibition of human influenza A virus replication by chloroquine" pot
Ngày tải lên :
20/06/2014, 01:20
... needed to determine the role, if any, of chloroquine in the prophylaxis and treatment against influenza infection It would also be interesting to determine the activity of chloroquine against the highly ... ment of influenza A (H5N1) infection since the pathology of avian influenza infection in humans appears to be mediated by pro-inflammatory cytokines [13] Although we have shown that chloroquine is ... antiviral stockpile against a possible pandemic In conclusion, chloroquine demonstrates an inhibitory effect against the replication of human influenza A virus H1N1 and H3N2, in vitro and further...
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báo cáo hóa học:" Augmented inhibition of angiogenesis by combination of HER2 antibody chA21 and trastuzumab in human ovarian carcinoma xenograft" pptx
Ngày tải lên :
20/06/2014, 07:20
... variety of signaling cascades, including the phosphatidylinositol-3 kinase (PI3K)/serine/threoninespecific protein kinase (AKT), and it regulates various cell functions especially in tumor growth, ... potency on inhibiting the AKT It may partly explain our findings that the combination of chA21 with trastuzumab could synergistically enhance the in vitro and in vivo anti-angiogenesis effects In conclusion, ... analyzed using Image-pro Plus 5.02 (Media Cybernetics Inc, USA) for VEGF expression MVD was determined by counting the number of microvessels (marked by CD34 staining) per high-power field (200×) in...
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Báo cáo khoa học: " A novel b-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages" docx
Ngày tải lên :
07/08/2014, 23:22
... LPS in vivo and on the in vitro mitogenic activity of LPS by forming a stable molecular complex with the lipid A of LPS [21] Therefore, this study also investigated the effects of polymyxin B ... of βglucan and LPS in order to exclude any possible contamination due to endotoxins during the preparation process Polymyxin B significantly (p ＜ 0.05) inhibited NO production by LPS actvation ... actvation Nevertheless, polymyxin B had no significant effect on NO production induced by β-glucan (Fig 4) Finally, the mRNA expression of various cytokines was investigated in RAW264.7 macrophages...
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Báo cáo y học: "Inhibition by Natural Dietary Substances of Gastrointestinal Absorption of Starch and Sucrose in Rats and Pigs: 1. Acute Studies" pot
Ngày tải lên :
08/08/2014, 16:23
... the blood glucose increased above baseline by an average of approximately 25 mg/dl in the pigs in the absence of the enzyme-inhibiting formula, with essentially no increase in blood glucose when ... and/or 100 g rice starch individually or combined in enough drinking water to solubilize the constituents This fluid mixture was consumed totally within minutes To complete an investigation on each ... amount of blood obtained at a single testing amounted to less than 0.5 ml Ingredients: The individual test ingredients as well as the formulation were obtained from AdvoCare International, Carrollton,...
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Báo cáo y học: "Inhibition by Natural Dietary Substances of Gastrointestinal Absorption of Starch and Sucrose in Rats 2. Subchronic Studies" pptx
Ngày tải lên :
08/08/2014, 16:23
... of combining natural products containing inhibitors of both starch and sucrose absorption was strengthened when a combined starch-sucrose challenge, simulating a meal containing both, was influenced ... [38] Because angiotensin can influence water intake [39], a decrease in circulating angiotensin might explain, at least in part, both the lower water intake and the decrease in SBP Dietary CHO have ... see a lower gain in the group receiving the formula Continuation of this study might have resulted in a significant difference When the drinking water was changed to 10% w/v sucrose in week 5, the...
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Báo cáo y học: "Modulation of granulocyte-endothelium interactions by antileukoproteinase: inhibition of anti-type II collagen antibody-induced leukocyte attachment to the synovial endothelium" ppt
Ngày tải lên :
09/08/2014, 08:22
... Figure by ALP Inhibition of IL-1β-induced upregulation of E-selectin in glEND.2 cells by ALP (a) Inhibition of IL-1β-induced upregulation of E-selectin in glEND.2 cells by antileukoproteinase ... off-line analysis ALP in vivo Inhibition of leukocyte interaction with the postcapillary endothelium by ALP in vivo (a) Inhibition of leukocyte rolling along the postcapillary endothelium by antileukoproteinase ... blocking effects of ALP on leukocyte rolling in vivo as an additional interference with selectin interactions beyond the already known suppression of leukocyte integrin activation [5] In an in vitro...
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Báo cáo khoa học: "Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo" docx
Ngày tải lên :
09/08/2014, 09:20
... proven effective in inhibiting the function of proteins by blocking the activity domain or critical protein interaction sites There have been numerous antibody drugs undergoing pre-clinical and Du ... DNA-PKcs is approximately 470 kD consisting of 4128 amino acids Its C-terminus contains a serine/threonine protein kinase domain (3719- 4128) [23] For screening Page of 11 the specific anti-DNA-PKcs ... for screening singlechain variable antibody fragment (scFv) In essence, scFv is a small protein made up of both variable heavy and light chain domains coupled by a flexible peptide linker, and...
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Báo cáo khoa học: " Differential protection by wildtype vs. organelle-specific Bcl-2 suggests a combined requirement of both the ER and mitochondria in ceramide-mediated caspase-independent programmed cell death" docx
Ngày tải lên :
09/08/2014, 10:20
... storage in the cell - has also been implicated in the induction of both apoptosis [15] and ciPCD [2,12,13] In fact, the ER may play a key role in certain types of ciPCD, as intracellular calcium influx ... cells stably expressing the various versions of Bcl-2 in pRc/CMV were obtained by electroporation using a Gene pulser II (BioRad) followed by selection with Geneticin (Invitrogen) and were used ... this treatment induces PCD exclusively by caspase-independent mechanisms, but not by apoptosis [31,32] In this study, we additionally verified this by measuring the activities of the initiator-caspase...
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Báo cáo y học: " Targeting viral infection by microRNA inhibition" pdf
Ngày tải lên :
09/08/2014, 20:21
... before treatment were accompanied by administration of an intravenous saline placebo SPC3649 was administered by weekly intravenous injection for 12 weeks followed by a 17-week treatment-free follow-up ... competitive inhibitors of small RNAs in mammalian cells Nat Methods 2007, 4:721-726 doi:10.1186/gb-2010-11-1-201 Cite this article as: Roberts APE, Jopling CL: Targeting viral infection by microRNA inhibition ... genomes is critical in vivo and suggesting that resistance to SPC3649 therapy might not be generated by mutation in miR-122 binding sites Rebound of viral load did not occur during therapy, and...
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