ca2 oscillations and ca2 waves

... Mechanically-induced Ca2+ waves in RTEC plated on collagen or LM-332/collagen Figure Mechanically-induced Ca2+ waves in RTEC plated on collagen or LM-332/collagen Pseudo-colour maps of increases in [Ca2+ ]i ... mechanically induced Ca 2+ waves and ATP-induced Ca 2+ oscillations in airway epithelial cells Am J Physiol 1999, 276:L669-678 Evans JH, Sanderson MJ: Intracellular calcium oscillations induced by ... RTEC propagated intercellular Ca2+ waves in response to mechanical stimulation on both matrices tested However, cells grown on LM-332/collagen matrix propagated Ca2+ waves via an extracellular nucleotide...

Ngày tải lên: 12/08/2014, 16:20

Ngày tải lên: 17/03/2016, 21:24

... 3rd, and 4th log cycle of inactivation, respectively; and are deduced from the regression line of their corresponding DRT curves To fit the model to the experimental data and to calculate d and ... the scale and shape parameters from the fitting of the Mafart equation to the survival curves to heat and ultrasound (MS and MTS) obtained in this study Root mean square error (RMSE) and determination ... to heat, MS, and MTS in apple juice Fig shows the C sakazakii inactivation rates by heat (dT values) and ultrasound under pressure at non-lethal (dMS values) and lethal Table Heat and MS/MTS resistance...

Ngày tải lên: 05/05/2014, 08:43

... s(t) and that xl and x2 are chosen so that x, >x2 Suppose p and q and their first derivatives are continuous in xx >x >s(t) and in s(t)>x> x2, and have finite limits as x—>s(t) from above and ... hyperbolic waves The most outstanding new phenomenon of the nonlinear theory is the appearance of shock waves, which are abrupt jumps in pressure, density, and velocity: the blast waves of explosions and ... Introduction and General Outline 1.1 1.2 The Two Main Classes of Wave Motion, Hyperbolic Waves, 1.3 Dispersive Waves, 1.4 Nonlinear Dispersion, 12 PARTI HYPERBOLIC WAVES 19 Waves and First Order...

Ngày tải lên: 12/06/2014, 16:26

... passive oscillations, recent experimental studies in isolated [14, 15] and non-isolated [16] saccular hair cells have documented spontaneous selfsustained voltage oscillations associated with Ca2+ and ... values of gh and gL result in slower- and larger-amplitude oscillations because of hyperpolarization of the cell and activation of the inward rectifier (K1) current The increase of gh and gL results ... a random Gaussian force that was band-limited to 200 Hz and had a standard deviation of σs = pN (b) Sensitivity as a function of the amplitude of the sinusoidal external force Values of b and...

Ngày tải lên: 20/06/2014, 23:20

... conventional way of expanding the land mass for land scarce countries such as Japan, the Netherlands, Monaco and Singapore is through aggressive land reclamation programs, However, land reclamation ... kilometer of the coast, the demand on land resources and space is beginning to approach a critical stage as the population continues to expand at an alarming rate and industrial and urban development ... of the box and always look at the ‘big picture’ which saved me from confusion and doubts in research and in my life I am grateful to all my friends and colleagues for their support and encouragement...

Ngày tải lên: 09/09/2015, 10:07

... ocean, including internal waves, inertial waves and sound waves, are not considered here Almost everyone has personal experience with water waves and sound waves But even for waves of small amplitude, ... 33 lands of Honshu and Hokkaido were struck by a wall of water 21-feet high and 197 people drowned It is clear from the above events that the tsunami waves are fairly permanent and powerful waves, ... dispersive waves, particularly solitary waves and solitons In particular, the Kortweg-de Vries family of nonlinear dispersive wave equations admit such solitary waves and solitons and describe...

Ngày tải lên: 06/06/2016, 13:52

... Synchronization of Ca2+ oscillations One reported means by which stores achieve local synchrony is by Ca2+ waves, a significant form of signaling in living organisms [20–22] Ca2+ waves are considered ... the release of Ca2+ from a dominant store, triggering Ca2+ -induced Ca2+ release from adjacent stores, and the continuation of this process along the array of stores However, Ca2+ waves propagate ... coupling between Ca2+ stores? Oscillating Ca2+ stores can interact by altering the phase of adjacent oscillators through Ca2+ -inducedCa2+ release Here, coupling by exchange of Ca2+ [and ⁄ or Ins(1,4,5)P3...

Ngày tải lên: 16/02/2014, 09:20

... propagating Ca2+ transients As shown in Fig 1Ba, which represents changes in [Ca2+ ]i in the neuron–astrocyte cocultures, astrocytes and neurons show Ca2+ oscillations with different frequency and temporal ... Pathology and Ca2+ transients in astrocytes Ca2+ waves in astrocytes propagate into neurons and affect the excitability of neurons via the release of neuroactive gliotransmitters such as ATP and glutaA ... However, Ca2+ waves are propagated between astrocytes even when the cells not have an absolute requirement for functional contact with each other directly, and the extent and direction of the Ca2+ ...

Ngày tải lên: 16/02/2014, 09:20

... that the Ca2+ mobilization (Ca2+ release from Ca2+ stores) and the synchronous Ca2+ oscillations are essential for neuroepithelial cell proliferation, for ventricular cell proliferation, and for ... provides the driving force for Ca2+ efflux, and the Ca2+ release may also be enhanced by Ca2+ -induced Ca2+ release Such fluctuations in the membrane potential of the Ca2+ store would continue as ... of the Ca2+ store [5] When the Ca2+ store is replenished with Ca2+ ions by Ca2+ pumps in the store membrane, and the store BK channels are reactivated, the voltage fluctuations of the Ca2+ store...

Ngày tải lên: 16/02/2014, 09:20

... the Ca2+ response relies on both phospholipase C and PI3-K activity PI3-Kb and not PI3-Kc mediates the P2Y12 effect on thrombin-evoked Ca2+ responses In man and mouse, the PI3-Kb (p110b) and ... autocrine-released and externally-added ADP reinforce the thrombin receptor-induced Ca2+ responses by a moderate increase of the first Ca2+ peak and a more marked increase of the later Ca2+ signal Furthermore, ... thrombin-induced Ca2+ response, and abolished the effects of ADP, AR-C and wortmannin A B C D Fig Contribution of SERCA and phospholipase C to P2Y12-dependent prolongation of Ca2+ responses (A,...

Ngày tải lên: 18/02/2014, 16:20

... °C with a Ca2+ -sensitive electrode (World Precision Instruments, Inc.) using calibration standards in the range from 10)8 to 10)1 M free [Ca2+ ] Results The Ca2+ -sensor proteins GCAP-1 and GCAP-2 ... HEK293 cells and COS cells Cell membranes were reconstituted with D6SGCAP-1 and GCAP-2 and the EC50 and IC50 values were determined EC50 values were similar as described previously [25,44] and maximal ... between GCAP-1 and GCAP-2 in their regulatory properties and if so, what is the physiological significance of such a difference? The study demonstrates that GCAP1 and GCAP2 modulate the Ca2+ signaling...

Ngày tải lên: 21/02/2014, 00:20

... lL HW-55S solution with Ca2+ and b-1,3-glucan (d), Ca2+ only (m), b-1,3-glucan only (j) and with neither Ca2+ nor b-1,3-glucan (s) than a combination of b-1,3-glucan and Ca2+ This result suggests ... Toyopearl CM-flow-through and Toyopearl CM-eluate were incubated in the presence of b-1,3-glucan and Ca2+ , PO activity was clearly shown to be dependent on b-1,3-glucan and Ca2+ (column 5) Tenebrio ... presence of b-1,3-glucan and Ca2+ (lane 11 in Fig 7B) The activation ratio of the 55-kDa zymogen form in the presence of b-1,3-glucan and Ca2+ was faster than in the presence of Ca2+ only (compare lane...

Ngày tải lên: 21/02/2014, 03:20

... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ... and repeated three times in the presence of Ca2+ we lysed the yeast cells in the presence of Ca2+ (10 mM) or EGTA (1 mM) and examined PtdEtn-PLD activity in the 100 000 g pellet (membranes) and ... Spo14p/Pld1p is Ca2+ -independent and thus may be assayed in the presence of EGTA and EDTA, conditions under which PtdEtn-PLD is inactive On the other hand, PtdEtn-PLD hydrolyses PtdEtn and thus may...

Ngày tải lên: 22/02/2014, 07:20

... isolated EF-hand motif of STIM1 undergoes Ca2+ -induced conformational changes and remains as a dimer in the absence and presence of Ca2+ Results and Discussion The isolated EF-hand motif from ... EF-hand from STIM1 binds to Ca2+ Y Huang et al Fig Grafting the helix–loop–helix EF-hand motif into CD2 (A) The sequence alignment results of calmodulin EF-hand III and the canonical EF-hand ... EF-hand binds to Ca2+ and lanthanide ions One of the most important steps to fully understand the mechanism underlying the Ca2+ -modulated functions of STIM1 is to investigate the site-specific Ca2+ binding...

Ngày tải lên: 07/03/2014, 00:20

... to Ca2+ -binding sites is known to generate the J-band (610–650 nm) and ⁄ or the c-band (480–510 nm) Upon binding to Calnuc, the dye displayed prominent J-band and c-band in both absorption and ... b-crystallin and d-crystallin behave similarly (show only one band), whereas calmodulin and troponin behave like each other On the other hand, Calnuc and parvalbumin generate both the J-band and the c-band, ... the c-band, respectively; with parvalbumin, however, the J-band is retained at all stoichiometries Also, whereas the J-band is replaced by the b-band and the c-band upon the addition of Ca2+ to...

Ngày tải lên: 07/03/2014, 00:20

... control H-89 was used to inhibit PKA activation It inhibits PKA I and II (IC50 ¼ 135 nm), and also ribosomal S6 kinase (S6K1) and mitogen- and stress-activated protein kinase (MSK1); with 10 lm of H-89 ... channels In this study, we show the involvement of L-type Ca2+ channels, protein kinase A (PKA), Ca2+ ⁄ calmodulin-dependent kinase II (CaMK II) and mitogen-activated protein kinases (MAPK ⁄ ERK) in ... at both concentrations of BAPTA-AM These results indicate that Ca2+ influx via the L-type channel and an increase in intracellular Ca2+ levels are involved in the action of neokyotorphin in L929...

Ngày tải lên: 07/03/2014, 11:20

... factors and their interplay, such as Ca2+ and ROS Therefore, we exposed isolated rat brain mitochondria to H ⁄ R and ⁄ or Ca2+ and determined H2O2 concentration, membrane permeability, and, as ... ⁄ R and Ca2+ Brain mitochondria ( 0.5 mg proteinÆmL)1) were incubated at 30 °C under the conditions indicated: Ca2+ , 15 in incubation medium; Ca2+ , 15 in incubation medium plus 3.5 lM Ca2+ ; ... (lane 2), 3.5 lm Ca2+ (lane 6), and the combination of both (lane 3) induced FEBS Journal 272 (2005) 3593–3601 ª 2005 FEBS L Schild and G Reiser Fig Effect of H ⁄ R and low micromolar Ca2+ on state...

Ngày tải lên: 16/03/2014, 22:20

... cyclic-3¢,5¢-AMP, NaF and prostaglandin E1 Endocrinology 86, 396–405 11 Corvilain, B., Van Sande, J., Laurent, E & Dumont, J.E (1991) The H2O2-generating system modulates protein iodination and the activity ... Lecomte, M., Van Sande, J & Dumont, J.E (1994) Role of the cyclic adenosine 3¢,5¢-monophosphate and the phosphatidylinositol -Ca2+ cascades in mediating the effects of thyrotropin and iodide on hormone ... 3000 g for 15 at °C and resuspended in mL 50 mM sodium phosphate buffer, pH 7.2, containing 0.25 M sucrose and mM MgCl2 (buffer A) The pellet was washed twice with mL buffer A and centrifuged at...

Ngày tải lên: 17/03/2014, 03:20

... duration, the actual Ca2+ uptake would be much lower Moreover, the low temperature- and Ca2+ -induced increase in kinase level could be blocked by EGTA and W7 (a CaM inhibitor) and the high Mg2+ ... Engineering and Biotechnology and funds from Department of Science and Technology, Government of India We thank Prof S Assmann for critical reading of the manuscript and constructive suggestions and ... (v/v) and collected by gentle centrifugation Nuclei were resuspended in washing buffer containing 110 mM KCl, 10 mM phenylmethanesulfonyl fluoride, and lgÆmL)1 each of antipain and leupeptin and...

Ngày tải lên: 18/03/2014, 01:20