ca2 influx and generation of rns ros
Báo cáo y học: " A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions" pptx
... analysis of clones of GC4 infected by Akv-Y and Akv-2XY Figure Flow cytometry analysis of clones of GC4 infected by Akv-Y and Akv-2XY The profile of AYGC.2A2 was representative of all Akv-Y infected ... expressing Env and EYFP The profile of A2XYGC.2B1 was representative of all Akv-2XY infected GC4 clones, except A2XYGC.3B8, in that they expressed high EYFP levels and GFP-Cre but no Env Of all the ... between the 5'LTR and gag and between the env and the 3'LTR (b) The Akv-Y provirus contained IRES-EYFP in the U3 region of the LTR (c) The Akv-2XY provirus contained IRES-EYFP and two LoxP sites...
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Synthetic progress toward parvistemonine, spiroxins a and b, and generation of palmarumycin analogues
... HOMOTYROSINE 25 3.0 OXIDATION OF HOMOTYROSINE 27 3.1 4.0 OXIDATION OF HOMOTYROSINE TO DIENONE 27 BIRCH REDUCTION OF HOMOTYROSINE 30 4.1 BIRCH REDUCTION OF HOMOTYROSINE AND ... oxidative cyclization of tyrosine was incorporated into the syntheses of several natural products, and the extension of this methodology to homotyrosine for construction of the azacyclic core ... Model of spiroxin core 293 215 xii LIST OF SCHEMES Scheme 1.1 Williams’ retrosynthetic analysis of (±) croomine (4) Scheme 1.2 Chen and Hart’s retrosynthetic analysis of stenine...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... thrombin-induced Ca2+ response, and abolished the effects of ADP, AR-C and wortmannin A B C D Fig Contribution of SERCA and phospholipase C to P2Y12-dependent prolongation of Ca2+ responses (A, ... equation of Fluo-3 for Ca2+ [27] Ultra-pure calcium-free water was used for preparation of all buffers and (ant)agonists Measurement of cytosolic cAMP and InsP3 Intracellular levels of cAMP and InsP3 ... also changes in [Ca2+ ]i-time integrals, which reflect both the extent and duration of the [Ca2+ ]i increase [9] Platelet pretreatment with AR-C reduced the Ca2+ peak (–9%) and the Ca2+ integral (–34%)...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ... chloride salts of divalent cations on the membrane-bound and cytosolic PtdEtn-PLD activities assayed in the absence of added EDTA and EGTA, i.e in the presence of 10)5 M of ambient free Ca2+ The ... and repeated three times in the presence of Ca2+ we lysed the yeast cells in the presence of Ca2+ (10 mM) or EGTA (1 mM) and examined PtdEtn-PLD activity in the 100 000 g pellet (membranes) and...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local artificial library design in (A) CDR loops of Fv and (B) BC, DE and FG ... between Thr25 and Glu26 Fv Fig Functionalization of small scaffold proteins by replacing a loop of the scaffold protein with a CDR loop of antibody fragments (A) Replacement of the candidate location ... sequences and small scaffold proteins, and as methods for designing high-quality libraries of small scaffold proteins Functionalization of small scaffold proteins by peptide grafting The design of chimeric...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... isolated EF-hand motif of STIM1 undergoes Ca2+ -induced conformational changes and remains as a dimer in the absence and presence of Ca2+ Results and Discussion The isolated EF-hand motif from ... affinity and to probe the oligomeric state of the EF-hand of STIM1 using size-exclusion chromatography and pulsed-field diffusion NMR We found that mutations on loop positions and of the EF-hand from ... perturb the Ca2+ -binding ability of the grafted EF-hand of STIM1 (B) Modelled structure of the engineered protein with the grafted EF-hand Ca2+ -binding motif (magenta) from STIM1 W32 and Y76 in...
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Báo cáo khoa học: Stimulation of fibroblast proliferation by neokyotorphin requires Ca2+ influx and activation of PKA, CaMK II and MAPK/ERK pdf
... target one of the elements of the PKA-signaling system PKA may activate the Ca2+ L-type channel, resulting in an increase in the Ca2+ influx, elevation of the intracellular Ca2+ level and CaMK II ... activation It inhibits PKA I and II (IC50 ¼ 135 nm), and also ribosomal S6 kinase (S6K1) and mitogen- and stress-activated protein kinase (MSK1); with 10 lm of H-89 the activity of these kinases is suppressed ... containing 10% of fetal bovine serum (Sigma), mm l-glutamine, 10% standard vitamins solution, 100 lmÆmL)1 of penicillin G, 0.1 mgÆmL)1 of streptomycin sulfate and 0.25 lgÆmL)1 of amphotericin...
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Báo cáo khoa học: "Bilingual Hebrew-English Generation of Possessives and Partitives: Raising the Input Abstraction Level" pptx
... partitives a loaf of bread, a slice of cake, and general partitives: a piece/bit /of an item of X In the syntactic structure of a partitive structure, the part is the head of the phrase (and determines ... amount of the thing The main modifications from the existing SURGE input structure are the introduction of the re/-set feature and the update of the usage of the possessor feature For both of these ... modifiers and determiners Machine Translation, 11:159-184 R Gedalia 1996 Automatic generation of business letters: Combining word-based and template-based nlg through the distinct handling of referring...
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Báo cáo khoa học: Modulation of Ca2+ entry and plasma membrane potential by human TRPM4b pptx
... (Icrac [14]) A Ca2+ -free ⁄ Ca2+ -readdition protocol was used to distinguish between the release of Ca2+ from intracellular stores and Ca2+ influx across the plasma membrane Whereas Ca2+ release ... influence of fused EGFP on channel function (compare Fig 1D with Fig 2A,B) Generation of HEK-293 clones with stable expression of TRPM4b To investigate the effect of TRPM4b expression on Ca2+ entry and ... Modulation of Ca2+ entry by TRPM4b R Fliegert et al phosphorylation by protein kinase C, and binding of Ca2+ –calmodulin and phosphatidylinositol 4,5-bisphosphate [8,9] Nilius et al observed Ca2+ -dependent...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... C roseus D A E B C F Fig GUS and GFP fluorescence patterns of CrPrx expression in N tabacum leaf (A) GUS staining and (B) GFP fluorescence patterns of the same (C–E) GFP fluorescence patterns of ... and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene The asterisk indicates the position of the codon encoding ... 4, and show PCR reactions run on plasmid DNA harboring CrPrx cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and...
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Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... Centruroides noxius Hoffmann were isolated and functionally characterized Cn2 is one of the most abundant and toxic components of C noxius venom (6.8% of total venom; LD50 ¼ 0.25 lg per 20 g of mouse weight) ... noxius venom To the best of our knowledge, this is the first report of the generation of a human recombinant antibody fragment capable of neutralizing the toxic effects of the whole venom from ... neutralize LD50 of toxin Cn2 and LD50 of whole venom Mutant 6009F was obtained after performing some modifications to the standard procedures of biopanning, specially the inclusion of a pre-elution...
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Báo cáo khoa học: "Syntactic Dependence and the Computer Generation of Coherent Discourse" pdf
... order of application of formulas It is a modification of a method used by Yngve13 and was suggested as one of several methods by Harris4 In the usual description of a phrase structure generation ... head of the verb phrase in which they appear Two-way dependency exists between the head of a phrase and any form of the verb "to be" or the preposition of. ” This rule holds for the heads of both ... exceptions and additional convenient rules of dependency, such as the two-way linkage that we use for “to be” and of, ” which will improve the operation of language process- KLEIN AND SIMMONS...
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Báo cáo khoa học: A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration pdf
... protein sequence with that of other species (Xenopus, Drosophila, mouse isoforms 1, 2, and 4, rat isoforms and 2, bovine isoforms 1, 2, 3, and 4, and human isoforms 1, 2, 3, and 4) is shown in Fig ... inhibitors of ANT have also been reported [37] Mindful of (a) the well-established ligand profile of ANT, (b) the modulatory role of ANT in the mammalian PTP, and (c) the absence of a Ca2+ -induced ... Fig TEM and EFTEM images of Ca2+ loaded Artemia mitochondria (A, B) TEM images of Artemia mitochondria loaded with Ca2+ , incubated in the absence (A) or presence (B) of ADP (C) TEM images of Artemia...
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Báo cáo khoa học: "DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION PROJECT" potx
... transfer and generation stages of our Mu system to make the system both powerful and fault-tolerant As is mentioned above, our system has many advantages such as the flexibility of the generation ... Grobal sentential structures of Japanese and English are quite different, and correspondingly the internal structure of a Japanese sentence is not the same as that of English Fundamental difference ... utilization of strong lexical information The system is in the course of development in collaboration with a number of computer scientists from computer industries and expert translators Some of the...
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Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx
... Sequence analysis Both CM-AAT1 and CM-AAT2 encode proteins of 461 amino acids with a theoretical molecular mass of 51.5 kDa and 51.8 and a pI of and 8.5, respectively, and 87% identity at the amino ... recombinant protein was capable of producing esters from a wide range of combinations of alcohols and acyl-CoAs with the exception of ethanol, nonanol, and linalol from acetylCoA, and ethanol, cis/trans-3-hexenol, ... trellis in a greenhouse under standard cultural practices for fertilization and pesticide treatments Flower tagging on the day of hand pollination and daily measurements of internal ethylene (WT fruit...
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Báo cáo Y học: Inhibition of SERCA Ca2+ pumps by 2-aminoethoxydiphenyl borate (2-APB) 2-APB reduces both Ca2+ binding and phosphoryl transfer from ATP, by interfering with the pathway leading to the Ca2+-binding sites ppt
... function of free [Ca2+ ] in the absence and presence of 2-APB Purified Ca2+ ATPase (0.5 lM) was incubated in a buffer at either pH 7.2 (A) or pH 6.0 (B) and the effects of different free [Ca2+ ] on ... Table ATP binding and phosphorylation of the Ca2+ -ATPase Figure 8A shows the effects of mM 2-APB on the phosphorylation of the Ca2+ ATPase in SR The EC50 and maximum level of phosphoenzyme formation ... to the Ca2+ ATPase (A) shows the effects of phosphorylation of the SR Ca2+ ATPase at varying concentrations of [c-32P]ATP, in the absence (j) and presence (s) of mM 2-APB (B) shows the spectroscopic...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... of antigen-specific CTL and the generation of Treg by fusions of DCs and HCC cells The local tumor-related factors may favor the generation of Treg through the inhibition of DCs maturation; however, ... high levels of IFN-γ production and lysis of the CEA- and/ or WT1-positive targets restricted in HLA-A2 and/ or HLA-A24 Interestingly, fusions of immature DCs generated in the presence of HCC cell ... supernatants (HCCsp) and allogeneic HCC (DCs/allo-HCC/sp) induce dysfunction of the fused cells and promote the generation of CD4+ CD25high Foxp3+ Treg and impair the induction of antigen-specific...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... DCTs and DCTIs have an optimal antigen uptake capacity and upregulation of CD86, CD40 and class I, but low levels of CD83, as is expected of non-terminally mature DCs The phenotypic profile of ... JME, ME: conception and logistics of the study MMA, CV, SS, RAR, SC AJM: vaccination of patients, acquisition of samples and generation of data MMA, AJM, NRH, JB, FF: preparation of the vaccine MMA, ... I and hTERT) Tetramer analysis allowed careful documentation and tracking of peptide-specific CD8+ T cells produced as a result of the in vivo immunizing activity of each type of DC Page 14 of...
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báo cáo hóa học: " Platelet-activating factor enhancement of calcium influx and interleukin-6 expression, but not production, in human microglia" pptx
... inhibition of SOC with SKF96365 blocked both the influx of Ca2+ and microglial expression of IL-6 However, PAFinduced expression of IL-6 (Fig 2) did not translate into production of the cytokine ... attributed the lack of a SOC phase of [Ca2+ ]i due to concomitant ATP binding to some P2XR (not P2X7R) causing cellular depolarization and block of Ca2+ influx [6] PAF induction of IL-6 was found ... PAF-induced Ca2+ responses PAF-induced Ca2+ responses A: Representative trace (n = 18 cells) showing change in [Ca2+ ]i induced by PAF (100 nM) Following a prolonged level of SOC-mediated influx of Ca2+ ,...
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Báo cáo hóa học: "Photochemically reduced polyoxometalate assisted generation of silver and gold nanoparticles in composite films: a single step route" pptx
... broad bands at 460 and 750 nm (Fig 2, curve b) When the reduced composite film was dipped into AgNO3, these bands disappeared and a new band at 420 nm is observed indicating the formation of Ag ... bands of Ag, Au nanoparticles The time evolution formation of Ag nanoparticles in the composite film was monitored by UV-Visible spectroscopy As the time of dipping increased, the blue color of ... suitable dipping time intervals and concentration of metal ions solutions As the concentration of AgNO3 is increased, the intensity of the surface plasmon band increases and the absorption shifts to...
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