ca2 independent nitric oxide synthase activation in endothelial cells
Báo cáo y học: " Protein kinase A-dependent Neuronal Nitric Oxide Synthase Activation Mediates the Enhancement of Baroreflex Response by Adrenomedullin in the Nucleus Tractus Solitarii of Rats" pps
Ngày tải lên :
10/08/2014, 05:21
... Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats Neuroscience 2009, 159:727-734 Prast H, Philippu A: Nitric oxide ... in NTS AC: adenylate cyclase; ADM: adrenomedullin; ADMR: adrenomedullin receptor; GC: guanylate cyclase; Gs: stimulatory GTP-binding protein; nNOS: neuronal nitric oxide synthase; PKA: protein ... of nitric oxide on baroreflex activation in the brainstem nuclei of rats Chin J Physiol 1996, 39:57-62 26 Pontieri V, Venezuela MK, Scavone C, Michelini LC: Role of endogenous nitric oxide in...
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Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc
Ngày tải lên :
22/03/2014, 17:20
... Rapamycin inhibits LPS-induced G-CSF and iNOS expression G-CSF promoter iNOS promoter Oct-2 Oct-2 IgG A β-actin Input LPS LY Akt -in Rapa LPS Rapa Fig Rapamycin inhibits the binding of Oct-2 to the iNOS ... factors binding to it in LPS-induced gene expression Although involvement of octamer-binding factor-1 (Oct-1), another octamer-binding protein, cannot be excluded in the LPSinduced expression of iNOS ... and by inhibiting eukaryotic translation initiation factor 4E-binding protein (eIF4E-BP1) [19] Rapamycin, a potent immunosuppressor, exerts its function by binding to the FK506-binding protein (FKBP12)...
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Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx
Ngày tải lên :
30/03/2014, 14:20
... the FAD isoalloxazine ring nNOS, rat neuronal nitric oxide synthase; eNOS, rat endothelial nitric oxide synthase; iNOS, rat inducible nitric oxide synthase; CPR, human cytochrome P450 reductase; ... F1395S NOS, suggesting interplay between CaM binding, nicotinamide binding and F1395 in coenzyme binding/FAD reduction [29] The switch in coenzyme specificity towards NADH in CPR is consistent ... indicating that Trp676 is actively involved in displacing NADP+ from reduced FAD domain [27] Mutagenesis of the aromatic shielding tyrosine residue in pea ferredoxinNADP+ reductase to serine stabilizes...
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Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx
Ngày tải lên :
07/08/2014, 18:21
... neuronal (nNOS), endothelial (eNOS), and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs The intensity of staining is indicated by (-), where staining was absent, ... of inducible and endothelial nitric oxide synthase in the rat ovary Effects of gonadotropin stimulation in vivo J Clin Invest 1995, 96, 2719-2726 15 Xie QW, Nathan C The high-output nitric oxide ... The involvement of nitric oxide in the ovulatory process in the rat Endocrinology 1994, 135, 2287-2290 11 Takesue K, Tabata S, Sato F, Hattori MA Expression of nitric oxide synthase- 3 in porcine...
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Báo cáo y học: " Inhibition or knock out of Inducible nitric oxide synthase result in resistance to bleomycin-induced lung injury" potx
Ngày tải lên :
12/08/2014, 18:21
... staining for nitrotyrosine (B) was localized mainly in nuclei of inflammatory cells There was a marked reduction in the immunostaining in the lungs of bleomycin-treated iNOSKO mice (C) and in ... the tissue injury caused by inflammation [26] Induction of lung injury by bleomycin Mice received a single intratracheal instillation of saline (0.9%) or saline containing bleomycin sulphate ... of aminoguanidine in a murine model of pulmonary fibrosis induced by bleomycin Fundam Clin Pharmacol 2000, 14:561-7 Hu J, Xu Q, Li B: The effect of aminoguanidine, a nitric oxide synthase inhibitor,...
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Báo cáo y học: "Signalling pathway involved in nitric oxide synthase type II activation in chondrocytes: synergistic effect of leptin with interleukin-1" pdf
Ngày tải lên :
09/08/2014, 06:22
... Ishimaru S, Hirata Y: Cytokineactivated Jak-2 is involved in inducible nitric oxide synthase expression independent from NF-kappaB activation in vascular smooth muscle cells Atherosclerosis 2002, ... were incubated for 48 hours with both cytokines in the presence of the NOS type II inhibitor aminoguanidine (1 mmol/l), added hour before cytokine administration Aminoguanidine completely inhibited ... accumulation in the culture supernatant of human primary chondrocytes (Fig 1c) and ATDC5 cells (Fig 1d) Janus kinase-2 inhibition blocks leptin/IL-1 induced nitric oxide production and nitric oxide synthase...
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Báo cáo y học: " Overexpression of endothelial nitric oxide synthase suppresses features of allergic asthma in mice" potx
Ngày tải lên :
12/08/2014, 16:20
... double-blind placebo-controlled study of the effect of inhibition of nitric oxide synthesis in bradykinin-induced asthma Lancet 1996, 348:374-377 Redington AE, Meng QH, Springall DR, Evans TJ, Creminon ... measured in lung tissue by the Larginine to L-citrulline conversion assay using a nitric oxide synthase assay kit (Calbiochem, La Jolla, CA, USA; cat no 482700) according to the manufacturer's instructions ... der Linde HJ, Nijkamp FP: Virus-induced airway hyperresponsiveness in guinea pigs is related to a deficiency in nitric oxide J Clin Invest 1995, 95:26-30 Persson MG, Gustafsson LE: Allergen-induced...
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Báo cáo y học: "Involvement of Akt and endothelial nitric oxide synthase in ventilation-induced neutrophil infiltration: a prospective, controlled animal experiment" docx
Ngày tải lên :
13/08/2014, 08:20
... effects on stretch-induced Akt and endothelial nitric oxide synthase activation Phosphorylated serine/threoactivation nine kinase/protein kinase B (Akt) or endothelial nitric oxide synthase (eNOS) ... also lead to activation of Akt and eNOS [14,15] Nitric oxide has been shown to be produced from Larginine by a family of nitric oxide synthase isoforms, including inducible nitric oxide synthase ... without hyperoxia are complex, including activation of mitogen-activated protein kinases [12], serine/threonine kinase/protein kinase B (Akt), and endothelial nitric oxide synthase (eNOS) [14,15] High...
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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf
Ngày tải lên :
19/02/2014, 16:20
... enrichment in iNOS activity within the particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... localization of iNOS to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... the C-terminus of iNOS promotes in vitro interactions with the PDZ protein EBP50 [13] Interestingly, EBP50 has different binding partners including ezrin that can be anchored to the actin cytoskeleton...
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Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
Ngày tải lên :
07/03/2014, 05:20
... lines on the autoradiogram The origin was marked with an ink spot Argininosuccinate is the immediate precursor to arginine in the urea cycle, and is converted to arginine and fumarate by argininosuccinate ... NOS assay Citrulline Hydroxyarginine Argininosuccinate Ornithine Origin 1-D 2-D Fig Two-dimensional TLC analysis of reaction product A reaction with [14C]arginine, desalted protein extract and ... arginine that mimics an NOS reaction This reaction, however, produces argininosuccinate, not citrulline, and requires fumarate, indicating that ASL is catalyzing the reaction Because argininosuccinate...
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Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx
Ngày tải lên :
16/03/2014, 13:20
... structures of peptides derived from the calmodulin-binding domains of inducible and endothelial nitric- oxide synthase in solution and in complex with calmodulin J Biol Chem 272, 23050–23056 18 Yuan T, ... Walsh MP (1998) Characterization of the Ca2+ -dependent and -independent interactions between calmodulin and its binding domain of inducible nitric oxide synthase FEBS Lett 431, 210–214 19 Meador ... demonstrating that electron transfer within the reductase domain of iNOS is CaM -independent using only the reductase domains of both human and mouse iNOS [29] In contrast with the results obtained...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... previously to induce an increase in the level of [Ca2+ ]i and a correlated calpain activation [28] This increase in [Ca2+ ]i is more intense in aorta than in brain Under these conditions, in brain, no ... of inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in ... al In vivo degradation of NOS and HSP90 by calpain higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following...
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báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
Ngày tải lên :
19/06/2014, 22:20
... cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells Diabetologia 1998, 41:1101-8 Kong GY, Peng ZC, Costanzo C, Kristensson K, Bentivoglio M: Inducible nitric oxide ... treated with increasing concentrations of TNFα in the presence A: absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated ... derived neurotrophic factor; IGF, insulin-like growth factor; IL-1β, interleukin-1beta; iNOS, inducible nitric oxide synthase; MAPK, mitogen activated protein kinase; NF-κB, nuclear factor kappa...
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Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
Ngày tải lên :
07/08/2014, 14:22
... the inflammatory cells in the pathogenesis of EAE, as does iNOS in EAE [45] However, our study did not support the finding of EAE in iNOS knockout mice in which both nNOS and eNOS were not increased ... of nitric oxide synthase in autoimmune encephalomelitis 27 28 29 30 31 32 33 34 35 36 37 38 Induction of nitric oxide synthase activity in human astrocytes by interleukin-1beta and interferon-g ... anti-digoxigenine antibody for 60 Positive cells were visualized using a diaminobenzidine substrate kit (Vector) and counterstained with hematoxylin Fig The clinical course of rat spinal cord homogenate-induced...
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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx
Ngày tải lên :
07/08/2014, 14:23
... observed in inflammatory microabscesses Counterstained with hematoxylin Magnification: ×132 C: Immunohistochemical staining of inducible NOS in the caprine brain with natural listeriosis shows that iNOS ... immunoreactivity was recognized in neurons, some inflammatory cells in perivascular cuffings Counterstained with hematoxylin, Magnification: ×132 D: Immunostaining of neuronal NOS in brain sections of goats ... of nNOS and iNOS in the nonneurological control brain Both nNOS and iNOS immunoreactivity was recognized in the brain of control goats No inflammatory cells were found in the brain sections of...
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Báo cáo khoa học: " Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex" doc
Ngày tải lên :
07/08/2014, 23:22
... apparently involved in neurodegeneration by different mechanisms, including oxidative stress and activation of intracellular signaling mechanisms [19] Al affects NO production in the brain Al decreases ... epinephrine autoxidation Anal Biochem 1978, 90, 81-89 35 Tanino H, Shimohama S, Sasaki Y, Sumida Y, Fujimoto S Increase in phospholipase C-delta1 protein levels in aluminum-treated rat brains ... group (Fig 1) Superoxide anion radical production in the rat forebrain cortex The results in Fig show the bilateral ∙O2 levels (μM red NBT/min/mg proteins) in the rat forebrain cortex homogenates...
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Báo cáo y học: "Octacalcium phosphate crystals directly stimulate expression of inducible nitric oxide synthase through p38 and JNK mitogen-activated protein kinases in articular chondrocytes" pps
Ngày tải lên :
09/08/2014, 06:23
... protein kinases and tyrosine kinases on IL1-induced NF-κB activation and iNOS expression in bovine articular chondrocytes Nitric Oxide 2002, 6:35-44 66 LaPointe MC, Isenovic E: Interleukin-1β ... dehydrogenase; iNOS, inducible nitric oxide synthase; JNK, c-Jun amino-terminal kinase; NO, nitric oxide; OCP, octacalcium phosphate Figure OCP crystal induced p38 and JNK MAPK activation Nonadherent bovine ... ankyrin repeat containing a serine/threonine protein kinase that interacted with the cytoplasmic domain of β1 integrin [51] Furthermore, Liu and Lioté [37] found that, within a few minutes, MSU crystals...
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Báo cáo y học: "Dynamic compression counteracts IL-1β induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs" pdf
Ngày tải lên :
09/08/2014, 10:23
... Nelson AH, Barone FC, Kumar S: SB203580 inhibits p38 mitogenactivated protein kinase, nitric oxide production, and inducible nitric oxide synthase in bovine cartilage-derived chondrocytes J Immunol ... noted for iNOS induction in response to IL-1β, with a sevenfold increase in free-swelling conditions (Figure 2a), as compared with a 145-fold increase in unstrained constructs cultured within the ... marked differences in the diffusional constraints in unstrained constructs in the bioreactor, involving contact of the upper surface with a fluid impermeable loading pin Accordingly, the mass transport...
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Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
Ngày tải lên :
09/08/2014, 14:20
... Kanno K, Saito I, Miyasaka N: Nitric oxide production and inducible nitric oxide synthase expression in inflammatory arthritides J Clin Invest 1995, 96:2357-2363 41 McInnes IB, Leung BP, Field M, ... OA in rabbits [8] and to improve the subchondral bone structure in an ovine OA model induced by meniscectomy [9] In addition to the above findings, and most interesting are the results from clinical ... Nitric oxide mediates interleukin-1 induced inhibition of glycosaminoglycan synthesis in rat articular cartilage Mediators Inflamm 1995, 4:107-111 48 Murrell GAC, Jang D, Williams RJ: Nitric oxide...
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