c adeno associated virus aav based vectors

... strains including C beijerinckii ATCC17778, C acetobutylicum DSM792 (ATCC824) or C acetobutylicum NI4082 (reclassified as C saccharoperbutylacetonicum) and C butyricum are non-pathogenic and their ... accomplishment are within close reach [25] (C) Adeno- associated virus (AAV) -based vectors AAV- based vectors have been shown to be non-toxic and undergo widespread cellular uptake in preclinical ... intravenous injection of rhabdomyosarcoma-bearing rats with at least 107 spores of C beijerinckii ATCC17778, C acetobutylicum DSM792 (= ATCC824) or C acetobutylicum NI-4082 (reclassified as C saccharoperbutylacetonicum)...

Ngày tải lên: 14/08/2014, 19:22

... TRAIL, CXCL1, CCL5, IFN-β and oncolytic viruses for the treatment of glioma, renal cell carcinoma, prostate cancer, lung cancer, and pancreatic cancer animal models (Hu et al., 2010) NSC vectors ... and cyclooxygenase-2 (Cox-2) (Pesonen et al., 2011) Besides oncolytic adenoviruses, several viruses such as oncolytic herpes simplex virus (HSV), vaccinia virus, reovirus, Newcastle disease virus, ... Type Culture Collection BV Baculovirus CD Cytosine deaminase CMV Cytomegalovirus CNS Central nervous system CRAds Conditionally replicating adenoviruses EGFP Enhanced green fluorescent protein...

Ngày tải lên: 09/09/2015, 10:08

... injection of BV-PEI25k particles…………………….136 XI ABBREVIATIONS AAV AcMNPV adeno- associate virus Autographa californica nuclear polyhedrosis virus Ad adenovirus BV baculovirus CAG cytomegalovirus ... infective viruses, such as Cytomegolovirus (CMV), Simian virus( SV40), CMV enhancer/chicken β-actin promoter (CAG) etc., have been commonly used However, the transcriptional activity of such strong ... easy comparing to other types of viral vectors, such as Lentivirus which needs cotransfection of a set of plasmids for each batch of virus production, or Adenoassociated virus which requires complicated...

Ngày tải lên: 14/09/2015, 11:28

... primer, 5-ATTGCGGCCGCGGTACCGAGCTCTTACG and reverse primer, 5-ATTGCGGCCGCTTATCGATTTTACCACATTTG The CMV E/PDGF promoter, CMV promoter and PDGF promoter were then cut from pGL3-basic plasmid and ... neurotrophic factor BV Baculovirus CAG CMV enhancer/β-actin promoter CMV Cytomegalovirus CMV E Enhancer of cytomegalovirus immediate-early gene CNS Central nervous system DMEM Dulbecco’s modified ... PCR in the DNA engine Opticon Thermal Cycler (MJ Research, USA) using the luciferase gene-specific primers 5’CGGGCGCGGTCGGTAAAG3’ and 28 Chapter Hybrid Neuron-Specific Promoter 5’ACTTCAGGCGGTCAACGAT3’,...

Ngày tải lên: 16/09/2015, 08:30

... pair Cell cycle-dependent element Cell cycle-dependent factor Cell cycle genes homology region Cytomegalovirus Epidermal growth factor receptor Fluorescence activated cell sorter Fas -associated ... Helper virus- free packaging system 20 Figure 3.1 Summary of cell cycle regulation 56 Figure 3.2 Cyclin A promoter sequence 59 Figure 3.3 Sequence alignment of the CDE/CHR region in the cdc2 5C, cdc2 ... analysis 5.3 Results 5.3.1 Cell type-specific and cell cycle-regulated transgene expression mediated by HSV-1 amplicon vectors in vitro 5.3.1.1 Cell type-specific and cell cycle-regulated transgene...

Ngày tải lên: 16/09/2015, 15:54

... http://www.translational-medicine.com/content/8/1/10 mechanism could provide a new target for anti-cancer drug development List of Abbreviations AAV: adeno- associated virus; CP: chronic pancreatitis; IFN: ... Adenovirus type wt from crude lysate titer and Ad DNA replication was determined by qPCR using the following primers: Ad type forward primer 5’-AACCGAAGGCTGCATTCACT, reverse primer 5’-ACCGCACAGGGTCTTAATAGAG ... [21] The 10 human PDAC cell lines included Panc1, MiaPaCa-2, HPAF-I, CFPAC1, Ger, PSN1, Panc2, Paca3, Paca44 and PT45 [22] Page of 11 total RNA according to manufacturer’s instructions (Affymetrix)...

Ngày tải lên: 18/06/2014, 16:20

... 4.11 Phase contrast images showing the cytotoxicity of 139 GCV in NSC1-tk cells and NSC1-tk cocultured with U87 Figure 4.12 In vitro therapeutic efficacy of NSC1-tk cells in the 140 coculture system ... cell MSC Mesenchymal stem cell EGF Epidermal growth factor bFGF Basic fibroblast growth factor EB Embryoid body SDF-1 Stromal cell-derived growth factor CXCR4 CXC chemokine receptor VEGF Vascular ... tumor-specific replication and lysis in early-phase clinical trials (Shah et al, 2003; Varghese and Rabkin, 2002) 1.2.2 Chemical vectors In clinical trials, chemical vectors have shown lower transfection...

Ngày tải lên: 11/09/2015, 09:00

... this case Bgl II and Xho I) of the destination vector (pGL4.11) InFusion Forward primer 5-’GCTCGCTAGCCTCGACCACTAGTAACGGCCGCCAGT- 3’  InFusion Reverse primer 5’-CGCCGAGGCCAGATCCCAGTGTGATGGATATCTGCA ... the cloning of the kb promoter region was kept the same IFI30 1.5 kb F-5’TGTAGGCCCACTCTATGCCTGGCACCA 3’ IFI30 kb F-5’-GTCTTGCCCTGAATGTAAGGCCTGGC-3’  IFI30 R -5’- CGGCTGCCTGGGAAATAAGCGCCTTTAAC-3’ ... ACGTATCGATACTTGGATCCTGGGTACCCTAGAGAAATGA-3’ IFI30 R NotI: 5'- ACTTGCGGCCGCTGCCTGGGAAATAAG-3' 28    Step Conditions Cycles 94 C, 2min 94 C, 30s 55 C, 30s 30 68 C, 45s 68 C, 10 4 C HOLD Table 2.4- PCR conditions for cloning...

Ngày tải lên: 22/10/2015, 21:20

... Genes Vsuc Vsuc Vacc Tmed -3 4V Text Vmed med Tacc 2048 Vext -3 Tsuc Tsuc 4096 -1 Vext Tacc Number of genes: Vacc -1 Text Vmed Baseline Classifier / SVM Genes 3 Vext -3 Tsuc Tmed Vacc 1024 512 ... results; suc = success rate; acc = acceptance rate; ext = extremal margin; med = median margin The coefficients have been rescaled such that each indicator has zero mean and variance across all four ... function/relation to Colon cancer Collagen alpha 2(XI) chain (Homo sapiens) Collagen is involved in cell adhesion Colon carcinoma cells have collagen degrading activity as part of the metastatic...

Ngày tải lên: 06/03/2014, 00:22

... human cervix, RCB accession no RCB0271) and CHO cells (a Chinese hamster ovary, RCB accession no RCB0285) (RIKEN Cell Bank, Tsukuba, Japan) HuH-7 cells (a human hepatoma cell line, JCRB accession ... polyampholyle comb-type copolymers consisting of poly (L-lysine) backbone and hyaluronic acid side chains for a DNA carrier Bioconjugate Chem 9, 476–481 Cationic -Helical Peptides 11 Cationic -Helical ... (step 2c) per well Incubate for h at 37 C in the CO2-incubator Cationic Methacrylate Polymers/DNA Complexes 53 Table Cell Viability Calibration Curve Cells/well A B C D E F G H 100 µL cell stock...

Ngày tải lên: 11/04/2014, 09:59

... Helper Virus- Free Production of Adeno- Associated Virus Vectors Roy F Collaco and James P Trempe 237 Novel Tools for Production and Purification of Recombinant Adeno- Associated Viral Vectors ... Missouri, Columbia, MO THOMAS W DUBENSKY, JR • Vice President, Research, Cancer Vaccines, Cerus Corporation, Concord, CA MOGENS DUCH • Departments of Molecular and Structural Biology and Medical Microbiology ... and Protocols consists of 30 chapters detailing the use of herpes viruses, adenoviruses, adeno- associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems...

Ngày tải lên: 11/04/2014, 10:39

... more datasets Abbreviations CaMP score: cancer mutation prevalence score; CAN-gene: candidate cancer gene; CAT plot: ‘correspondence at the top’ plot; FDR: false discovery rate; GBM: glioblastoma ... G, Velculescu V: A multidimensional analysis of genes mutated in breast and colorectal and colorectal cancers Genome Research 2007, 17:1304-1318 The Cancer Genome Atlas Research Network: Comprehensive ... or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript...

Ngày tải lên: 09/08/2014, 22:23

... more datasets Abbreviations CaMP score: cancer mutation prevalence score; CAN-gene: candidate cancer gene; CAT plot: ‘correspondence at the top’ plot; FDR: false discovery rate; GBM: glioblastoma ... G, Velculescu V: A multidimensional analysis of genes mutated in breast and colorectal and colorectal cancers Genome Research 2007, 17:1304-1318 The Cancer Genome Atlas Research Network: Comprehensive ... or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript...

Ngày tải lên: 09/08/2014, 22:23

... μg/ml PEI was cytotoxic, significantly reducing cell viability However, the observed cytotoxicity decreased as the PEI concentration decreased Cytotoxicity was Control 97% SSC A undetectable with ... cancer gene-specific for ALCL The nanocomplexes are specific and noncytotoxic to lymphoma cells, which advance great potential for their clinical applications Page 10 of 12 structure (nanocore) was ... indicated Reduction of the PEI-medicated non-specific cell binding was then monitored by flow cytometry B, To gain specific cell binding capacity, the CD30 aptamer was incorporated into PEI-citrate...

Ngày tải lên: 11/08/2014, 00:22

... 5'GCTTTTCCTGCGGTATTCCTG-3'; Mmp7 forward, 5'CTGATGATGAGGACGCAGGA-3', Mmp7 reverse, 5'ATTCATGGGTGGCAGCAAAC-3'; Svs2 forward, 5'-TCAGAAAGGCCGTCTCAGTT-3', Svs2 reverse, 5'-AGCTGCTTCGTCACTTCCTC-3'; ... 5'-AGGAGCGATTTGCTGGTGTGGA-3', S16 reverse, 5'-GCTACCAGGCCTTTGAGATGGA-3'; Psca forward, 5'GCCTGGTAGAGGCTGAGATG-3', Psca reverse, 5'ATCATCTCCTGGGACTCCTG-3'; Clusterin forward, 5'TTTATGGACACAGTGGCGGA-3', Clusterin ... responsible for cancer susceptibility have successfully identified modifying loci [12] The characterization of specific genes modulating cancer phenotypes indicates that carcinogenesis is influenced by...

Ngày tải lên: 14/08/2014, 07:21

... retroviral vectors for gene therapy Non-defective oncogenic retroviruses Non-defective, replication competent retroviruses are also associated with malignant diseases These viruses not carry oncogene ... non-defective simple retroviruses are overtly oncogenic and the oncogenic, non-defective simple retroviruses show a spectrum of tissue specificity and oncogenic potential Analysis of the oncogenic ... for vectors developed from particular retroviruses, such as Friend virus Retroviruses causing CNS disease Several retroviruses cause CNS disease Some of these, such as the murine retroviruses Cas-Br-E...

Ngày tải lên: 14/08/2014, 19:22

... PV: 5' CGG GAT CCG GCC AGC TCT CAC AGT CCG GT; DuvSAF: 5' CGG GAT CCC TCT CAC TCC CTT GTT GAT GG; EBL1: 5' CGG GAT CCT GCT TAT GAC TCA CAA GTA GT; LagNGA: 5' GGA ATT CTT GTT ACC ATG AGT CAA CTA ... CAC CAT GCC ACT CAA TGC AGT CA (sense), 5' CGG GAT CCC TCT CAC TCC CTT GTT GAT GG (antisense); EBL1: 5' GGA ATT CAC CAT GTT ACT CTC TAC CGC CA (sense), 5' CGG GAT CCT GCT TAT GAC TCA CAA GTA GT ... respectively The primers used for PCR were as follows: Rabies PV: 5' GGA ATT CCA AGG AAA GAT GGT TCC TCA G (sense), 5' CGG GAT CCG GCC AGC TCT CAC AGT CCG GT (antisense); DuvSAF: 5' GGA ATT CAC CAT...

Ngày tải lên: 14/08/2014, 19:22

... 5´–CTC AGC GGC ATC CGC ACC CCT C 3´ For BMP6, the upstream primer was 5´–TTAGAT CTC CAC CAT GCC CGG GCT GGG G–3´, and the downstream primer was 5´–AGA ATC ACA GCC CCT GCA A–3´ Single PCR products ... BMP4, the upstream primer was 5´–CCA CCA TGA TTC CTG GTA ACC GAA TGC TG–3´, to which CCACC was added to give the amplified product a typical Kozak consensus sequence around the initiator methionine; ... were transfected into 293A cells with Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA) African green monkey kidney cells (Vero cells, No CCL-81; American Type Culture Collection [ATCC], Manassas,...

Ngày tải lên: 31/10/2012, 17:08

... screening - http://thomsonlifestylecentre.com/breast-cancer-screening-package The American Cancer Society recently launched new cancer of the breast screening recommendations The brand new recommendations ... additionally to some mammogram, not instead of Learn more at cancer screening http://thomsonlifestylecentre.com/breast-cancer-screening-package ... each year Women at moderate risk (15 to 19 percent) should request their healthcare provider concerning the benefits and drawbacks of adding a yearly MRI There's no proof that MRI is an efficient...

Ngày tải lên: 27/01/2014, 08:52