... follows: In vitro transcription and translation and immunoprecipitation Plasmids, containing cDNA of the antigens were purified with Qiagen miniprep kit (Qiagen GmbH, Hilden, Germany) The construction ... hearing loss Laryngoscope 1984, 94:501-507 Boulassel M-R, Tomasi J-P, Deggouj N, Gersdorff M: Identification of beta-actin as a candidate autoantigen in autoimmune inner ear disease Clin Otolaryngol ... Immunological disturbances have previously been described in Turner syndrome, with an association to reduced levels of serum IgG and IgM, increased IgA and decreased levels of circulating T- and...
Ngày tải lên: 11/08/2014, 08:21
báo cáo khoa học: "Correction: Addison’s disease presenting with idiopathic intracranial hypertension in 24-year-old woman: a case report" doc
Ngày tải lên: 11/08/2014, 02:22
Báo cáo y học: "Addison’s disease presenting with idiopathic intracranial hypertension in 24-year-old woman: a case report." pptx
... Wysowski DK, Fleming GA, Stadel BV: Benign intracranial hypertension in children with growth hormone deficiency treated with growth hormone J Pediatr 1995, 126(6):996-999 Leggio MG, Cappa A, Molinari ... 250 mg daily for presumed idiopathic intracranial hypertension (IIH) Visual acuity and visual field (Goldman perimetry) testing was unremarkable On endocrine review, generalised hyperpigmentation ... although she had no buccal pigmentation, she did have marked pigmentation of a recent scar over her left shoulder (Figure 1) A short synacthen (250 mcg) test (SST) was performed and demonstrated...
Ngày tải lên: 11/08/2014, 11:23
Cushing’s Disease in Children: Report of Three Cases potx
... Case This girl presented at the age of 11 years and months She had experienced rapid weight gain from 31 kg to 65 kg, but had only grown by cm in height in years Acne and facial flushing were also ... A girl aged 10 years and months experienced rapid weight gain from 23 kg to 36.4 kg, with an increase in height of only 3.5 cm in years No specific drugs had been prescribed previously Although ... (Figure 3) Transsphenoidal pituitary adenomectomy was performed The pathologic findings disclosed an ACTH-secreting pituitary adenoma Transient neurologic diabetes insipidus was noted after surgery...
Ngày tải lên: 22/03/2014, 10:20
Báo cáo khoa học: LRRK2 in Parkinson’s disease: in vivo models and approaches for understanding pathogenic roles pdf
... Mouse Tetracycline-regulated transgenics ⁄ human LRRK2 G2 019S BAC transgenics ⁄ human LRRK2 R1441C Mouse Mouse Mouse One line expressing FLAG tagged LRRK2 wild-type (> 20-fold) shows regulated ... usefulness in studying gene function in vivo, particularly in the central nervous system [44] Growing evidence demonstrates the power of this transgenic approach in conferring correct transgene expression ... LRRK2 Worm [25] Transgenic expression of green flourescent proteintagged LRK-1 wild-type or G1 876S mutant Disruption of endogenous LRK-1 Transgenic expression and disruption of endogenous dLRRK Worm...
Ngày tải lên: 23/03/2014, 04:20
báo cáo khoa học: "Fulminant Leptospirosis (Weil’s disease) in an urban setting as an overlooked cause of multiorgan failure: a case report" pptx
... vancomycin was continued The right upper quadrant sonogram showed thickening of the gallbladder wall Urine legionella antigen and serum HIV antibody results were negative Initial blood cultures ... last longer than one month During this stage, specific organ damage can be observed Aseptic meningitis is one of the most important clinical syndromes that can occur in 80% of patients during the ... Mandell GL, Bennett JE, Dolin R Philadelphia: Churchill Livingstone Elsevier; , 2010:3059-3065 Chang ML, Yang CW, Chen JC, Ho YP, Pan MJ, Lin CH, Lin DY: Disproportional exaggerated aspartate transaminase...
Ngày tải lên: 11/08/2014, 02:22
Báo cáo khoa hoc:" Exclusion of PINK1 as candidate gene for the late-onset form of Parkinson''''s disease in two European populations" pps
... 5'-CTCTAGTGCCCCTGGAGAGCT-3' F 5'-CGAGTCTCCTGCATTCAGTGG-3' R 5'-GACATAGCAGGGCCTCTCAGAG-3' F 5'-TCAGGTGATGTGCAGGACATG-3' R 5'-CAGAGGTTTCTACCCACACCG-3' F 5'-GGACCAGAGAAGGGAAGACCC-3' R 5'-TCACGACACAGAGGATGCCA-3' 387 240 ... F 5'-GCTTACAAGGAACTTACCATTCTGC-3' R 5'-GTGCTGAGGACATAAGTGATGGAT-3' F 5'-GATGTATCAGCTCCAGGCCCT-3' R 5'-TATTCTTTCCAGGTGTTGTATCTGATG-3' F 5'-AAACGTATTGGGAGTCGTCGA-3' R 5'-CTCTAGTGCCCCTGGAGAGCT-3' ... PINK1 gene analysis Exon Primer sequence Product size (bp) Ex 507 Ex Ex Ex Ex Ex Ex Ex F 5'-AAGTTTGTTGTGACCGGCG-3' R 5'-CTTAGCTCCGTCCTCCGCT-3' F 5'-CCTTCCTAGGCTCCCTGGC-3' R 5'-AAGATGGGCATTTTGAGAACATCT-3'...
Ngày tải lên: 11/08/2014, 08:20
Báo cáo y học: "Manifestations of Ollier’s disease in a 21-year-old man: a case report" pdf
... problems are progressive shortening of the involved extremity, angular deformity, pathological fractures and malignant transformation in 20% to 50% of cases [2,3] There may be some gait problems ... correction of angular deformities and limb-lengthening procedures [2] Radionuclide bone scanning is one method used to assess lesions depicted on radiographs or magnetic resonance images that are ... and the possibility of malignant transformation was ruled out based on the pathology findings Conclusion Figure Bone scan of the same patient showing areas of radiotracer uptake in the humerus,...
Ngày tải lên: 11/08/2014, 14:20
Báo cáo y học: " Synchronously diagnosed eosinophilic granuloma and Hodgkin''''s disease in a 12-year-old boy: a case report" pptx
... D, Ehninger G: Simultaneous occurrence of Hodgkin's lymphoma and eosinophilic granuloma: a potential pitfall in positron emission tomography imaging Clin Lymphoma 2002, 3:121-4 Kjeldsberg CR, Kim ... cells including many eosinophils with Langerhans cells (Figure 1) Langerhans cells showed somewhat glassy pink cytoplasms, indistinct cell borders and longitudinal coffee-bean grooves in their ... flexion but no other abnormality on general physical examination No lymphadenopathy or organomegaly was detected Hematologic investigations showed hemoglobin of 11.6 g/ dl with MCV = 86, platelet count...
Ngày tải lên: 11/08/2014, 19:21
Báo cáo y học: " Accidental Jorge Lobo''''s disease in a worker dealing with Lacazia loboi infected mice: a case report" doc
... mass resembling a lipoma, with a smooth bright surface Because of the initial diagnosis of a benign tumor, microbiological testing (including culture) was not requested Histopathological examination ... pathogen, and transmission between susceptible hosts seems to be its survival strategy [6] However, several lines of evidence suggest that L loboi is acquired either through contact with propagules ... killing the host might have had a significant role in shaping its genome during its evolutionary path to a more restricted mammalian pathogen Conclusion This is a report of animal to human transmission...
Ngày tải lên: 11/08/2014, 20:20
Heart Disease in Pregnancy - part 1 doc
... volume of distribution of drugs together explain in large part the need for modified drug dosing during pregnancy Anatomical changes during pregnancy The physiological changes in preload and afterload ... arrhythmias during pregnancy Other changes, such as small pericardial effusion, may have little clinical significance Although the prognostic significance of changes such as ventricular remodeling are well ... vasodilatation Pathological volume-loaded conditions such as aortic or mitral regurgitation, or left-to-right shunting should be distinguished (Table 3.1) The mitral closure sound may be slightly increased...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 2 ppt
... during pregnancy, particularly when there is associated aortic regurgitation.4 Aortic regurgitation tends to be progressive in patients with tetralogy of Fallot who have not undergone surgery ... at 3.2 kg Eight women had unrepaired tetralogy at the time of their 20 pregnancies: of them were cyanotic during 12 pregnancies Unrepaired tetralogy was predictive of low infant birthweight, as ... edema, high diaphragms and poor operating conditions for the surgeon Every measure should be taken to avoid cardiac surgery during pregnancy However, when cardiac surgery cannot be avoided, management...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 3 pdf
... moderate degree of mitral regurgitation (Table 8.1).12 During pregnancy Mitral valve prolapse is well tolerated during pregnancy, even when there is mitral regurgitation.15 The main physiological ... of long-standing aortic or mitral regurgitation complicated by severe left ventricular dysfunction may decompensate during pregnancy The prognosis and management of such patients during pregnancy ... Sabag T, Al Zaibag M et al Beta-adrenergic receptor blockade in the management of pregnant women with mitral stenosis Am J Obstet Gynecol 1990;163: 37–40 Bates SM, Greer IA, Hirsh J, Ginsberg JS...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 4 pot
... mechanism of degenerative mitral regurgitation • The use of strict echocardiographic criteria avoids over-diagnosis • Echocardiographic examination plays a key role in quantifying mitral regurgitation ... mitral regurgitation by transthoracic echocardiography using standardized imaging planes: diagnostic accuracy and outcome implications J Am Coll Cardiol 2005;46:302–9 23 Otto CM Timing of surgery ... requiring cooperative management by several specialists during pregnancy Improved medical and surgical management permits affected women to reach childbearing age, but good advice should begin during...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 5 potx
... Hughes GRV Treatment of pregnancy loss in Hughes syndrome: a critical update Autoimmunity Rev 2002;1:298–304 81 Bates S, Greer IA, Hirsh J, Ginsberg JS Use of antithrombotic agents during pregnancy ... or diffusing capacity Lung mechanics The lung volume is not affected until the second half of pregnancy when the uterus enlarges, raising intra-abdominal pressure and altering the configuration ... trimester.44 The patient was obese (weight 155 kg, BMI 55) and gained 12 kg during pregnancy There was a past history of heavy snoring, with daytime somnolence getting worse in the first two trimesters...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 6 ppt
... performed during pregnancy in the rare patient with a large outflow gradient who develops severe intractable symptoms during pregnancy Table 13.1 General recommendations for management of patients ... therapy and surgical delivery have also been suggested.21,22 The hemodynamic burden is greater during pregnancy than in the puerperium Multiple pregnancy magnifies this If twin pregnancy is a cause ... there is hesitation over management and coronary angiography is delayed or not done The diagnosis will be confirmed by ECG changes, echocardiography, coronary angiography and release of cardiac...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 7 doc
... during pregnancy despite continuing therapy that had previously been effective.4 The greatest risk of drug-induced congenital malformation occurs during fetal organogenesis, which occurs during ... occurring during pregnancy, the numbers distributed throughout, but the most dangerous period still being the first 24 hours after delivery (vaginal or surgical), and the heightened risk continuing ... in pregnancy: importance of pregnancy induced changes in the vessel wall and bicuspid aortic valve in pathogenesis Br J Obstet Gynaecol 1994;101:1085–18 14 Basso C, Morgagni GL, Thiene G Spontaneous...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 8 pptx
... Ampicillin g i.v./i.m plus gentamicin 1.5 mg/kg i.v (max 120 mg) load Followed h later by ampicillin g i.v or amoxicillin g p.o Penicillin allergies: vancomycin g i.v over 1–2 h; gentamicin 1.5 mg/kg ... CT nor MRI are needed if leg vein ultrasonography is positive Pulmonary angiography Pulmonary angiography is safe during pregnancy with suitable abdominal screening, but is rarely indicated except ... Peripheral Follow management strategy for stable patients Central Catheter fragmentation Successful Unsuccessful Thrombolysis Surgical embolectomy Figure 17.5 Management strategy in patients with...
Ngày tải lên: 13/08/2014, 17:22
Heart Disease in Pregnancy - part 9 pps
... seeing these patients before they plan their pregnancy Genetic counseling is not just a case of providing a risk figure and arranging the necessary scans It may involve making the diagnosis of a genetic ... fluoroscopy time Transesophageal ultrasonography or even simple transthoracic ultrasonography (when a good echo window in the supine position can be achieved) can be used during percutaneous balloon ... child-bearing at an older age and the ongoing effects of cigarette smoking, stress and cocaine use, the occurrence of AMI during pregnancy is expected to increase Most commonly, AMI happens during the...
Ngày tải lên: 13/08/2014, 17:22