các rủi ro trong đầu tư

... cross-linking (ICLs), revealed a broad spectrum of anti-cancer activities in vitro without cross-resistance to taxol or vinblastine Due to BO-1051’s hydrophobic ability, it can penetrate through ... BO-1051-induced tumor growth delay in GBM-3 xenografts Treatment group Tumor growth period, days* Control 20.2 Absolute growth delay† BO-1051 29.5 9.3 IR 23.6 3.4 BO-1051+IR 36.2 16 Normalized growth delay‡ ... 6A For each group, the time for tumors to grow from 150 to 1500 mm (i.e., a 10-fold increase in tumor size) was calculated using tumor volumes from the individual mice in each group (mean + SD)...

Ngày tải lên: 09/08/2014, 09:20

... T: Transforming growth factor beta derived from bone matrix promotes cell proliferation of prostate cancer and osteoclast activation-associated osteolysis in the bone microenvironment Cancer Sci ... TNFalpha/TNFR/TRAF1 and IL-6/CD126/JAK/STAT [30,31] Osteoblast activity is strongly regulated by surrounding pH and growth factors released from resorbed Table Alkaline phosphatase specific activity of MG-63 ... detected with peroxidase-coupled secondary antibody and enhanced chemiluminescence (Cell Signaling Technologies, Beverly, MA) The bands were visualized by nitroblue tetrazolium/5-bromo-4chloro-3-indolyl-phosphate...

Ngày tải lên: 20/06/2014, 04:20

... T: Transforming growth factor beta derived from bone matrix promotes cell proliferation of prostate cancer and osteoclast activation-associated osteolysis in the bone microenvironment Cancer Sci ... TNFalpha/TNFR/TRAF1 and IL-6/CD126/JAK/STAT [30,31] Osteoblast activity is strongly regulated by surrounding pH and growth factors released from resorbed Table Alkaline phosphatase specific activity of MG-63 ... detected with peroxidase-coupled secondary antibody and enhanced chemiluminescence (Cell Signaling Technologies, Beverly, MA) The bands were visualized by nitroblue tetrazolium/5-bromo-4chloro-3-indolyl-phosphate...

Ngày tải lên: 20/06/2014, 07:20

... Germany) About 30 μg of total protein from each cell lysate was separated on a 10% NuPAGE Bis-Tris (Invitrogen) gel that was placed in an X-Cell SureLock Mini-Cell (Invitrogen) The membrane was blocked ... 2006, 29(1):1-13 Kessler JH, Mullauer FB, de Roo GM, Medema JP: Broad in vitro efficacy of plant-derived betulinic acid against cell lines derived from the most prevalent human cancer types Cancer ... Chintharlapalli S, Papineni S, Ramaiah SK, Safe S: Betulinic acid inhibits prostate cancer growth through inhibition of specificity protein transcription factors Cancer Res 2007, 67(6):2816-2823 17 Wick...

Ngày tải lên: 09/08/2014, 09:21

... S: The potential role of basic fibroblast growth factor in the transformation of cultured primary human fetal astrocytes and the proliferation of human glioma (U-87) cells Neurosurgery 1995, 37:723-730 ... apoptosis: Cytochrome C, Caspase3, and Bax As shown in Figure 4B, the level of Cytochrome C, Caspase3, and Bax was markedly higher in the Ad-bFGF-siRNA group than in the control and Ad-GFP groups, confirming ... residues Tyr705 and Ser727 are phosphorylated [16] For this propose, we extracted total proteins from DMSO, Ad-GFP, and Ad-bFGF-siRNA treatment groups at 24, 48, and 72 h time points and Figure Ad-bFGF-siRNA...

Ngày tải lên: 10/08/2014, 10:21

... IL-8 (Zaporozhets et al., 2004) Increased production of pro-inflammatory cytokines can promote development of inflammatory reaction at the early stages of infection process, produce neutrophil inflow ... Huntington's disease, and amyotrophic lateral sclerosis) (Galea et al., 1992) 3.2 Regulation of iNOS expression in glial cells Cloning of iNOS promoter from different species has strongly contributed to ... senile plaques trigger reactive glial changes and neuroinflammation that can contribute to neuronal loss through overproduction of reactive proinflammatory cytokines such as TNF-α, IFN-γ and IL-1β,...

Ngày tải lên: 13/11/2014, 06:42

... group (control); (b) group exposed to 0.5 ppm ozone (ozone group); (c) group exposed to 1.0 mg NNK/kg body weight (NNK group); (d) group exposed to 5,000 ppm DBP (DBP group); (e) group exposed ... colonies were propagated sufficiently for molecular analysis by RT-PCR using the propagation procedure for mouse clones described elsewhere [24] Propagated mutant T-cell clones from the control and ... (ozone + NNK group); (f) group exposed to 0.5 ppm ozone + 5,000 ppm DBP (ozone + DBP group); (g) group exposed to 0.5 ppm ozone + 1.0 mg/kg NNK + 5,000 ppm DBP (three-combination group) Exposures...

Ngày tải lên: 07/08/2014, 18:20

... -6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester (DCF-DA) was purchased from Molecular Probes (USA) Hydrogen peroxide solutions were obtained from Sigma-Aldrich (USA) Phospho-p38 ... increase in the ROS generation and LPO Fig Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced apoptotic cell death A: Dichlorofluorescein-sensitive cellular ROS was measured ... that the Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 253 Fig Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced decrease...

Ngày tải lên: 07/08/2014, 20:23

... electron to make molecular oxygen, resulting in excess superoxide production in most tissues [2] These superoxide anions are converted to hydrogen peroxide and water with the help of a group ... Dermatol 1990, 126, 213-221 Trojanowski JQ Rotenone neurotoxicity: a new window on environmental causes of Parkinson’s disease and related brain amyloidoses Exp Neurol 2003, 179, 6-8 Varshneya ... ＜ 0.05) Significant increase in lipid peroxidation indicated lipid membrane damage from 30 days onward Pyrethroids are metabolized in liver via cytochrome P450 oxidative pathways yielding reactive...

Ngày tải lên: 07/08/2014, 23:22

... [17,20] The treatment protocols for these groups are as follows Group (vehicle control) rats received 1X phosphate buffer saline ml/kg, s.c., twice a day from E3 to E20 Group (morphine) rats received ... compared to saline group, p < 0.05 **Significantly different compared to saline group, p < 0.01 ***Significantly different compared to saline group, p < 0.001 group and the saline controls (Figure 2B) ... dose reached mg/kg Group (methadone) rats received methadone, mg/kg, s.c., twice a day from E3 to E20 Group (buprenorphine) rats received buprenorphine, mg/kg, s.c., once a day from E3 to E20 The...

Ngày tải lên: 10/08/2014, 05:21

... two groups (n = 24) One group was infected with H1N1 virus and the other was uninfected Six weeks later, both groups were divided into subgroups (n = 6) In both groups, three of the subgroups ... partial intersubtypic cross-protection mainly relies on the cell-mediated immune responses induced by infection Though the cross-protection provided by infection could play a role in alleviating ... group *Significant difference (p < 0.05), compared with the corresponding H5N1 virus group Chang et al Virology Journal 2010, 7:197 http://www.virologyj.com/content/7/1/197 Page of Table Protection...

Ngày tải lên: 12/08/2014, 04:20

... cellular necrosis and IL-8 production, while decreased VEGF production, cell proliferation and IL-10 production Interestingly, preincubation with rIL-10 before hyperoxia protected FATIICs from injury ... analog 5-bromo-2’-deoxyuridine (BrdU) 65%-hyperoxia decreased type II cell proliferation by 36% after 24 h (control = 6.5 ± 0.25 vs hyperoxia = 4.2 ± 0.20; p < 0.01) and by 56% after 36 h (control ... and hyperoxic conditions, and TNFa-release decreased significantly in hyperoxic samples compared to controls (24 h-control = 17.3 ± 1.80 vs 24 h-hyperoxia = 9.7 ± 0.53; p < 0.01; 36 h-control =...

Ngày tải lên: 12/08/2014, 13:22

... QuantiFERON TB-Gold assay (QTF-G), RD1 intact proteins and selected peptides Moreover to evaluate whether or not a previous infection could influence such response we included a control group composed ... all enrolled individuals provided written informed consent prior to screening procedures, as did the parents of the children included in the study TST TST was administered by the Mantoux procedure ... subtraction of the appropriate control according to the described criteria [16] Cut-off values were determined by constructing a Receiver Operator Characteristic (ROC) curve by means of LABROC-1 software...

Ngày tải lên: 12/08/2014, 15:20

... is known about the processes that are involved in the RTG program, however deposited research The return to growth (RTG) transcription program To try and infer the stage through which the cells ... Such genes could be classified into two groups First, a 'process-linked' group was defined Genes in this group were induced during late meiosis in SPM (at around 8-9 hours), and were also induced ... alternative developmental route Differentiation processes proceed through a sequence of events involving coordinate modulations of morphology and gene expression In many cases, once the process has passed...

Ngày tải lên: 14/08/2014, 16:21

... increased compared to control group The proportion of early and late apoptotic cells in the 150 μM treatment group was about six times higher than in the drug-free group The proportion of apoptotic ... (50 μg) from each sample were subjected to seperate on a SDS-PAGE After electrophoresis, proteins were electroblotted to polyvinylidene difluoride membranes The membranes were blocked at room temperature ... glycerol, 200 mM KCl, 0.5 mM EDTA, 0.5% NP40, 0.5 mM DTT, 1% protease inhibitor cocktail) Protein concentration was determined by bicinchoninic acid (BCA) protein assay Equal amounts of protein...

Ngày tải lên: 10/08/2014, 10:21

... distribution, and reproduction in any medium, provided the original work is properly cited This is an Openlicenseefrom: http://www.retrovirology.com/content/7/1/59 Retrovirology available BioMed ... HU/UV promotes protein degradation of Cdc25A through Chk1 Vpr Promotes Proteasome-mediated Protein Degradations of Cdc25C and Cdc25B Since Vpr had little or no effect on Chk1-mediated Cdc25A protein ... a rather strong reduction of Cdc25C over time (Figure 5A-a, second row, lanes 8-10) To ascertain Vprmediated reduction of Cdc25C protein levels is also through proteasome-mediated proteolysis,...

Ngày tải lên: 13/08/2014, 01:20

... proliferation was calculated by (Abs cells – Abs background) treated/(Abs cells – Abs background) control X 100 80 60 40 20 -20 CellCell Proliferation Proliferation (% of control) (% of control) ... previously study showed that proteolysis of 14-3-3σ by Efp promotes breast tumor growth and suggested that 14-3-3σ protein might play an important role in regulating tumor growth [24] Conclusion In ... soluble β-glucans from Saccharomyces cerevisiae could activate macrophage to secrete TNF-α [6], which is found to have a pro-apoptotic effect on human cancer cells [7] CKBM is a product that contains...

Ngày tải lên: 02/11/2012, 11:12

... positive control a well characterized Rous Sarcoma Virus (RSV) promoter, excised from pAdRSVbgal, was inserted into pGFL, resulting in pRSVGFL and sequenced This promoter stems from rous sarcoma ... activity using the b-Gal staining kit purchased from Invitrogen Routine luciferase activity measurement procedures were performed according to the following procedure Cells were plated at a density of ... activity of the wild-type Egr-1 promoter construct was roughly 10±12% of the activity displayed by a control RSV promoter As demonstrated in Fig 2, the relative activity of promoter variant pD1egrGFL...

Ngày tải lên: 17/03/2014, 17:20

... reasons novel therapeutic approaches are strongly needed Mitochondria-directed chemotherapy is emerging as a promising tool to combat apoptosis-resistant cancer cell proliferation [10-12] Mitochondria ... acid (BA), a natural product derived from the bark of the white birch tree [25], has been demonstrated to potently inhibit the growth of neuroectodermal tumors, such as neuroblastoma, medulloblastoma, ... one WHO grade IV GBM sample were obtained from patients enrolled in a clinical-genetic protocol at Neurosurgery Unit of Livorno Hospital after the approval of the ethics review committee of Livorno...

Ngày tải lên: 18/06/2014, 15:20

... sequences of 3b protein may localize in the C-terminal regions from 134 to 154 amino acids [9] Nucleolus localization of SARS-CoV 3b protein suggested that the expression of 3b protein may interfere ... herpesvirus U(S) ORF8 protein induces apoptosis in infected cells and facilitates virus egress Virology 2002, 304:24-32 Drosten C, Gunther S, Preiser W, van der Werf S, Brodt HR, Becker S, Rabenau ... J, Cong Y: Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus Virus Res 2005 in press Chau TN, Lee KC, Yao...

Ngày tải lên: 19/06/2014, 08:20