các đề thi tuyển sinh 2002 2014

... hypoxia, and this increase was significantly inhibited by 10 μM DCQ (Figure 2B) This is in contrast to the observed increase in HIF-1a in response to μM or 10 μM DCQ under normoxia in this cell ... effects have been observed in EMT6 mouse mammary carcinoma cell lines [11] For this, we used the p53+/+ cells as model, since this cell line harbors functional p53 and DCQ significantly decreased the ... normoxic and hypoxic environments which explains the low HCR ratios of (< 1.5) specific for this cell line This is in contrast to the high HCR ratios (> 100) in T-84 human colon cancer cells [4],...

Ngày tải lên: 09/08/2014, 09:20

... a palindromic trioctamer of this sequence can interfere with CREB binding, and specifically inhibit PKA subunit expression, interfering with the CRE-PKA pathway [17] This causes specific inhibition ... cells, but significantly reduced cell numbers in GEO cultures Also, this effect was greater than expected (hyper-additive) (Fig 2A) This can be illustrated most clearly with the results for GEO cells ... for G1/S transition Cells arrest if this complex is not formed It has been suggested that CDO reduces pRb hyper-phosphorylation through p53 stabilization [18] This would cause an increase in p21waf1...

Ngày tải lên: 07/03/2014, 15:20

... account for this, but it may well result from a combination of processes, perhaps including loss of the N¢-terminal methionine ()131 Da) and oxidation of some or all of the remaining five methionines ... GST–Lck was purified using glutathione-linked resin, washing in buffer A (25 mM Pipes/NaOH, 500 M NaCl, pH 6.8), and then eluting the bound GST–Lck in 20 mM glutathione (reduced) in buffer A, before ... phosphorylation (six phosphates) was seen However, this analysis does not give any information about the location of the phosphate groups within the protein To determine the phosphorylation status...

Ngày tải lên: 08/03/2014, 02:20

... enzymatic function or regulation The formation of protein disulfides is catalysed by thiol–disulfide oxidoreductases through thiol–disulfide exchange reactions These enzymes have the capacity to oxidize, ... [23] In this connection, peroxiredoxin IV is emerging as a key player in dealing with the consequences of disulfide bond formation in the ER [24] N Bullied (Manchester, UK) described how this protein ... potential of the ER by cycling between the cysteine thiol form and the hyperoxidized cysteine sulfenic acid (–SOH) form The molecular details of how this enzyme operates alongside oxidative and reductive...

Ngày tải lên: 29/03/2014, 22:21

... mutations in NOTCH1 To investigate this further, we collected additional mutation data from public databases for T-ALL cell lines (Additional File 1, Table S4) For this dataset, a notable association ... phenotype useful system for this purpose [34] However, most of the hematological cell lines in our panel exhibited high sensitivity using proliferation as a measure of response This sensitive response ... ploidy content in the cell subpopulations in this tumor type, we observe high ploidy content in numerous B-cell lymphoma lines (e.g REC-1, 25% polyploidy) This further underscores the significance...

Ngày tải lên: 18/06/2014, 22:20

... is also a potent monocyte chemokine homologous to human MCP-1 with 66% amino acid identity [29] This study shows that HIF-1α is involved in transcriptional activation of MCP-1 and MCP-5 expression ... Institute, Montreal, Quebec The use of primary fetal human astrocytes was approved by the Research Ethics Board of National Research Council of Canada The FHAs cultures were prepared using the same ... resulting PCR was electrophoresed on 1.2% agarose gels in Tris-borate buffer containing 0.5 μg/ml ethidium bromide (Sigma), and then photographed The PCR generated a 504 DNA fragment for human and...

Ngày tải lên: 19/06/2014, 22:20

... mutations in NOTCH1 To investigate this further, we collected additional mutation data from public databases for T-ALL cell lines (Additional File 1, Table S4) For this dataset, a notable association ... phenotype useful system for this purpose [34] However, most of the hematological cell lines in our panel exhibited high sensitivity using proliferation as a measure of response This sensitive response ... ploidy content in the cell subpopulations in this tumor type, we observe high ploidy content in numerous B-cell lymphoma lines (e.g REC-1, 25% polyploidy) This further underscores the significance...

Ngày tải lên: 20/06/2014, 04:20

... in GCTs This pathway, in concert with other factors such as TGF-b and MCP-1, may stimulate the recruitment and cell proliferation of OPCs into GCTs, resulting in tumor progression In this study, ... and inserted between the two chambers Then, the cells were allowed to migrate for h at 37°C After this incubation period, the cells that had migrated to the lower side of the filter were fixed, ... treatment stimulates the tyrosine phosphorylation of Flt-1 in RAW cells, a model of OPCs [9] In this study, we used OPCs derived from rat bone marrow cells (rOPCs) [17] We previously reported...

Ngày tải lên: 20/06/2014, 04:20

... in GCTs This pathway, in concert with other factors such as TGF-b and MCP-1, may stimulate the recruitment and cell proliferation of OPCs into GCTs, resulting in tumor progression In this study, ... and inserted between the two chambers Then, the cells were allowed to migrate for h at 37°C After this incubation period, the cells that had migrated to the lower side of the filter were fixed, ... treatment stimulates the tyrosine phosphorylation of Flt-1 in RAW cells, a model of OPCs [9] In this study, we used OPCs derived from rat bone marrow cells (rOPCs) [17] We previously reported...

Ngày tải lên: 20/06/2014, 07:20

... post-irradiation but this response is abrogated in cells treated with loratadine Discussion In this study, treatment with loratadine enhanced the cytotoxic effect of radiation This effect was both ... (Figure 3) This recruitment is downregulated within 24 hours as DNA repair is completed in cells exposed to radiation alone In loratadine treated cells, however, there is a persistence of this signal ... at 1, 6, and 24 hrs after irradiation, increased within hr after irradiation and returned to baseline by 24 hrs Loratadine treatment enhanced this expression at hr and resulted in persistent expression...

Ngày tải lên: 09/08/2014, 10:20

... Tumor specimen The EGFR positive cell is defined as having clearly shown brownish yellow granules within cytoplasm and cell membrane; the COX-2 positive cell having clearly shown brown granules in ... 15 cases were staged I-II and 35 III-IV based on the revised AJC staging for lung cancer (1997) Thirty-nine cases had intra-thoracic lymph node metastasis (N1-N2), and 11 were negative lymph node ... and COX-2 expression might correlate with poor disease prognosis and reduced survival [20,24] In this study the prognostic values of EGFR and COX-2 were evaluated with immunohistochemical assay...

Ngày tải lên: 10/08/2014, 10:21

... Journal of Experimental & Clinical Cancer Research 2011, 30:27 doi:10.1186/1756-9966-30-32 Cite this article as: Li et al.: Correction: EGFR and COX-2 protein expression in non-small cell lung...

Ngày tải lên: 10/08/2014, 10:21

... profiles of target genes A number of genes, including cyclinD1, are regulated by ER alpha[7] In this study, two shRNA plasmid vectors against MTA1, which could persistently generate siRNA inside ... transfection), the second group was negative control(transfection with empty vector pGenesil-1, pG), the third group was pGM1(transfection with the first pGenesil-1/MTA1shRNA), the forth group was pGM2(transfection ... PCR fragments were visualized by UV illumination (Gel Doc 1000, BIO RAD corp, USA) stained with ethidium bromide The fluorescence intensity of 18SrRNA fragments served as the criterion for MTA1,...

Ngày tải lên: 10/08/2014, 10:21

... Cultures were studied three times weekly for 10 days to identify the presence of a cytopathic effect If a cytopathic effect, indicating the presence of HSV-1, was apparent or otherwise at days and ... (PLI) could help in determining the extent of tissue injury, as was previously described [18–20] This radionuclide technique involves gallium-67-labelled transferrin (67Gatransferrin) and technetium-99m-labelled ... HSV-1/HSV-2 typing kit, Palo Alto, CA, USA) In the four patients studied, the results were available within days after samples had been inoculated in culture medium On the day of specimen collection...

Ngày tải lên: 12/08/2014, 20:20

Ngày tải lên: 21/06/2013, 01:26

... Give the past form of these verbs: be tell begin think was, were told began thought have get live help had got lived helped Unit 1: A day in the ... day in the life of Period 4: Writing Task Task 2: The conclusion: The pilot announced that everything was all right, we landed safely 1 Task Task black smoke cough Unit 1: A day in the life of ... toward / fire exits / One door / be / block / Many people / begin /cough / choke Then / just as we / think / we / have / only / minutes / live, fire brigade / arrive Firemen / fight / their way / into...

Ngày tải lên: 18/09/2013, 05:10

... you have a daily routine? -is it same or different from Linh? 5ms 2.Pre-task: BRAIN STORM -ask ss think about some their activities in a day in m -after m t call on some ss and ask them about their...

Ngày tải lên: 02/12/2013, 19:11

... 22 Activity 5.1: Identifying Keys in the Logical Model Exercise 1: Identifying Keys In this exercise, you will identify primary, foreign, and composite keys for a logical data model based ... ∞ Job Purchases Client Is Sent To Bills Time To ∞ JobDesc TaskDesc Name Address City State Zip THIS PAGE INTENTIONALLY LEFT BLANK ...

Ngày tải lên: 21/12/2013, 06:16

... K R Wolthers and N S Scrutton Formation of holo-methionine synthase Co Homocysteine Methionine Cob(I)alamin Methylcob(III)alamin Primary turnover cycle AdoHyc AdoMet ... methionine synthase reductase (MSR) with methyl transfer from AdoMet [3] Most structural and functional information on hMS is derived by comparison with Escherichia coli cobalamin-dependent methionine ... Consistent with this, the crystal structure of the cobalamin-binding domain FEBS Journal 276 (2009) 1942–1951 ª 2009 The Authors Journal compilation ª 2009 FEBS 1943 Formation of holo-methionine synthase...

Ngày tải lên: 18/02/2014, 08:20