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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf

Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf

... region was constructed by ampli- fying the whole plasmid pEU-scFvLH by inverse PCR with the primers s2: 5¢- CAAAAAATTGAATGGCATG AACCGCCGAGCTCCAAC-3¢ and a2 : 5¢-AGCTTCAA AAATATCATTTAAACCCGACGGGCTGCTTTT-3¢ (the ... disulfide-containing protein through a batch cell -free system from wheat germ Takayasu Kawasaki 1 , Mudeppa D. Gouda 1 , Tatsuya Sawasaki 1,2 , Kazuyuki Takai 1,2 and Yaeta Endo 1,2 1 Cell -free Science and ... was amplified on the pEU–scFvLH using SP6 primer (5¢-ATTTAGGTGACACTATAG-3¢)andanti-pri- mer (5¢-ATGGCGCCAGCTGCAGGCTA-3¢, anti-stop codon in bold), and transcribed in the same way as above. Translation...

Ngày tải lên: 16/03/2014, 23:20

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Building wordpress themes from scratch

Building wordpress themes from scratch

... 'Primary Sidebar', 'primary-sidebar' ), 'id' => 'primary-widget-area', 'description' => __( 'The primary widget area', 'dir' ... it is automatically loaded during WordPress initialization (both for admin pages and external pages). ” 13 You can add features like sidebars, navigation menus, thumb- nail support, and more. ... page as a template in the WordPress editor: This template will supersede any other page-specic template that can be applied to the page. For example, if our page ID is 13, and we have a page-13.php...

Ngày tải lên: 18/04/2014, 11:32

141 373 1
OReilly.Building.a.Web.2.0.Portal.with.ASP.NET.3.5.Jan.2008-BBL

OReilly.Building.a.Web.2.0.Portal.with.ASP.NET.3.5.Jan.2008-BBL

... can add more widgets from a widget catalog and decorate the page as they like. How an Ajax-Powered Start Page Is Different The advantages of Ajax and a rich client-side experience give users a ... any part of the Start page asynchronously and give any web site an Ajax look-and-feel. However, UpdatePanel s are a significant drag on the page. The more UpdatePanel s you have, the slower asynchronous ... the SharePoint Portal, then you already know about widgets, which are called Web Parts in SharePoint and ASP.NET 2.0. Specifically, an Ajax-powered web portal is a web portal that uses Ajax technologies to...

Ngày tải lên: 15/11/2012, 14:24

310 488 1
Building a Sample Application Using ASP.NET AJAX

Building a Sample Application Using ASP.NET AJAX

... 10:47 AM Page 239 226 CHAPTER 10 ■ BUILDING A SAMPLE APPLICATION USING ASP.NET AJAX Figure 10-1. An ASP.NET AJAX-based stock application Understanding the Application Architecture The application ... have added AJAX capabilities to this application, and the page will not blink as it obtains data and renders the updates. You can see that there is no explicit coding for a partial-page update ... you looked at a real-world application and how you would implement it as an AJAX applica- tion quickly, simply, and powerfully using ASP.NET AJAX. CHAPTER 10 ■ BUILDING A SAMPLE APPLICATION USING ASP.NET...

Ngày tải lên: 05/10/2013, 10:20

44 497 0
LINUX FROM SCRATCH

LINUX FROM SCRATCH

... 103 Installing Man 104 Installation of Man 104 Contents 104 Description 104 man 104 Linux From Scratch viii Table of Contents Linux From Scratch 1 Gerard Beekmans 1 Dedication 2 Preface 10 Who ... want to read this book Users who don't want to build an entire Linux system from scratch probably don't want to read this book. If you, however, do want to learn more about what happens ... there is a lot of duplicate information in certain parts, it's the easiest way to read it and not to mention the easiest way for me to maintain the book. Part I − Introduction Part One gives...

Ngày tải lên: 24/10/2013, 01:15

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The Practical Guidelines for Building a Business Plan in Five Pages

The Practical Guidelines for Building a Business Plan in Five Pages

... anticipated that catches management unprepared. A contingency plan should address such occurrences. Natural disasters are a primary contingency that companies plan for. Like manmade situations, these ... viable method to increase the skill levels of your man- agers and employees goes a long way to creating a success from the plan. Building your education and training as an adjunct to the plan- ning ... brief- ing. Make sure participants understand that your planning model is about to take a dramatic turn for the better. Business-as-usual can- not be allowed. Building a Business Plan in Five Pages 39 Figure...

Ngày tải lên: 24/10/2013, 09:20

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Lab 5.1.11 Building a Peer-to-Peer Network

Lab 5.1.11 Building a Peer-to-Peer Network

... Layer 3, so that they can communicate. This lab does not require a hub or any other interconnecting network device. A basic CAT 5/5e UTP crossover cable is all that is needed. A crossover cable ... accomplished using a cat 5 or 5e crossover cable. Locate a cable that is long enough to reach from one PC to the other, and attach one end to the 3 - 4 CCNA 1: Networking Basics v 3.0 - Lab 5.1.11 Copyright ... directly connected. The default Gateway is only required on local area networks that are connected to a router. Computer IP Address Subnet mask Default Gateway PC – A 192.168.1.1 255.255.255.0...

Ngày tải lên: 27/10/2013, 07:15

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Lab 5.1.12 Building a Peer-to-Peer Network

Lab 5.1.12 Building a Peer-to-Peer Network

... directly connected. The default gateway is only required on local area networks that are connected to a router. Computer IP Address Subnet mask Default Gateway PC – A 192.168.1.1 255.255.255.0 ... two PCs a. Set the IP address information for each PC according to the information in the table. b. Note that the default gateway IP address is not required, since these computers are directly ... restored at the end of the lab. These include IP address, subnet mask, default gateway, and DNS servers. If the workstation is a DHCP client, it is not necessary to record this information. ...

Ngày tải lên: 27/10/2013, 07:15

4 476 0
Lab 5.1.13a Building a Hub-based Network

Lab 5.1.13a Building a Hub-based Network

... accomplished using a Category 5 or 5e straight-through patch cable. Locate two cables that are long enough to reach from each PC to the hub. Attach one end to the NIC and the other end to a port on ... to as a multiport repeater. Hubs are inexpensive and easy to install, but they permit collisions to occur. They are appropriate for a small LAN with light traffic. In addition to the physical ... ping command Background / Preparation This lab focuses on the ability to connect two PCs to create a simple hub-based Ethernet LAN using two workstations. A hub is a networking concentration...

Ngày tải lên: 04/11/2013, 16:15

4 353 0
Lab 5.1.13b Building a Switch-based Network

Lab 5.1.13b Building a Switch-based Network

... the two PCs and the switch will be accomplished using a Category 5 or 5e straight-through patch cable. Locate two cables that are long enough to reach from each PC to the switch. Attach one end ... Layer 3, so that they can communicate. Since this lab uses a switch, a basic Category 5/5e UTP straight-through cable is needed to connect each PC to the switch. This is referred to as a patch ... dedicated bandwidth to workstations. Switches eliminate collisions by creating microsegments between ports to which the two workstations are attached. They are appropriate for small to large LANs...

Ngày tải lên: 05/11/2013, 12:15

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