... Antonella Forlino for helpful suggestion and criticism, Elena Campari and Luigi Corazza for technical assistance, ÔCentro Grandi StrumentiÕ, University of Pavia, for peptide sequencing and free ... N-terminal sequencing for some peptides and for conformation by means of CD spectroscopy Chemical modification of collagens and CNBr peptides Chemical modifications have been performed with three different ... (lanes and 2) and recombinant decorin (lanes 3–4) we have used in this work, both before (lanes 1,3) and after (lanes 2,4) chondroitinase ABC digestion About 10 lg and lg were analyzed for decorins...
Ngày tải lên: 21/02/2014, 15:20
... 2-mercaptoethanol and 20% glycerol was added to the incubation mixture and then heated for at 100 °C Samples (50 lL) were submitted to SDS ⁄ PAGE (10% gel) [40], and western blot was performed as indicated ... [B] ⁄ [F] ¼ k(Bmax – B), where [B] is the bound ligand concentration, [F] is the free ligand concentration, Bmax is the maximum ligand amount, and k is the affinity constant light signal (7–8-fold) ... (unpublished work) In addition to Ca2+ and calpastatin, a number of digestible substrates can behave as calpain ligands and may accordingly induce changes in the conformation of the protease detectable...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
... and functional BMP ⁄ activin pathway in lophotrochozoans, for which a singular and promiscuous type II receptor would be the shared interface for both BMP and activin ligands Results Type I and ... pleiotropic and promiscuous receptor Evidence for the conservation of the mechanism of mesoderm induction in molluscs and across bilaterians Although Lartillot and colleagues have observed for a number ... already defined by the ligands and suggests a concerted evolution between ligands and receptors [23] According to the phylogenetic tree shown in Fig 2B, type (I or II) and subtype (TGF-b s.s.,...
Ngày tải lên: 07/03/2014, 21:20
REGIONAL HEALTH PRIORITIES AND OPPORITIES: ''''EVIDENCE FOR ACTION IN THE CHANGING GLOBAL FINANCIAL SITUATION'''' ppt
... Rwanda Ministry of Health Rwanda Ministry East African Community Kigali Health Institute Rwanda Biomedical Center RWANDA: GEOgrAPHY AND TOUrISM main theme: Regional health priorities and opportunities: ... Quality management and improvement initiatives Accreditation of health facilities and institutions Safety for service providers and patients Quality control systems for medicines and medical equipments ... e) Cancers f) Trauma and disabilities iii) Health systems strengthening a) Human resources for health (innovations and challenges) b) Healthcare financing c) Information and Communication technologies...
Ngày tải lên: 24/03/2014, 00:20
signature in the cell dna and the evidence for intelligent design
... no evidence for design in biology and, therefore, no good evidence for the existence of God According to Oxford evolutionary biologist Richard Dawkins and other New Atheists, the lack of evidence ... galaxies and particles of dust in the same terms because they both have mass and charge and length and width [But] you can’t that with information and matter.” A blank magnetic tape, for example, ... of evidence: the information—the digital code—stored in DNA and the other large biological molecules The case I make for intelligent design is less well known than Professor Behe’s and, therefore,...
Ngày tải lên: 08/06/2014, 09:00
báo cáo khoa học: " Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine" pptx
... design; performed the genotyping, phenotyping, sequence and database analyses; performed marker design and statistical, linkage and QTL analyses; and designed and drafted the manuscript BS and MG ... (down to 1.5 cM for SFW and 4.5 cM for BW, Table 1) and more reliable confidence interval (based on co-dominant markers) was established for the major QTL identified on LG 18 for seed and berry size ... (Figure 1B and 1C, and Table 1) Parametric QTL analyses (IM and MQM) did not reveal significant differences between the parental genotypes in any of the evaluated seasons (2007, 2009, and 2010) for...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo khoa học: "Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana" docx
... including Fort Collins and Ault (north-central), Wray (northeast), Fort Lewis and Breen (southwest), Nathrop (central), and Mesa (west-central) (Figure 1) Habitats at the Fort Collins and Wray ... of7neutrality (Fu and Li, 1993) for the M and S segments F test of neutrality (Fu and Li, 1993) for the M and S segments Only sequences from clade of the M tree (Figure 3) and clades – of the ... Center for Infectious Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, U.S Centers for Disease Control and Prevention, Atlanta, Georgia, for his insightful and very...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo khoa học: " Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana" docx
... including Fort Collins and Ault (north-central), Wray (northeast), Fort Lewis and Breen (southwest), Nathrop (central), and Mesa (west-central) (Figure 1) Habitats at the Fort Collins and Wray ... of7neutrality (Fu and Li, 1993) for the M and S segments F test of neutrality (Fu and Li, 1993) for the M and S segments Only sequences from clade of the M tree (Figure 3) and clades – of the ... Center for Infectious Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, U.S Centers for Disease Control and Prevention, Atlanta, Georgia, for his insightful and very...
Ngày tải lên: 12/08/2014, 04:22
Báo cáo y học: "The SLC2A9 non-synonymous Arg265His variant and gout; evidence for a population-specific effect on severit" docx
... Zealand (NZ) Māori, Pacific Island and Caucasian samples Methods Rs3733591 was genotyped across gout patients (n=229, 232 and 327, for NZ Māori, Pacific Island and Caucasian, respectively) and ... Caucasian cluster was 0.95 for the 505 Caucasian controls and 0.06 for the total 572 Māori samples, 0.95 and 0.06 for the 608 Eastern Polynesian samples and 0.98 and 0.05 for the 330 Western Polynesian ... Material and methods Study participants Genotyping of New Zealand (NZ) sample sets of Māori (229 cases and 343 controls), Pacific Island (232 cases and 174 controls) and Caucasian (327 cases and 638...
Ngày tải lên: 12/08/2014, 15:23
Báo cáo y học: "Computational and transcriptional evidence for microRNAs in the honey bee genome" pot
... (Biorad), and 0.2 μM of each forward and reverse primer The thermal program for all reactions was 95°C for 30 s followed by 40 cycles of (95°C for 30 s, 60°C for 30 s, 72°C for 30 s, 76°C for 10 ... and queen samples for six miRNA candidates Normalized expression across worker and queen samples for six miRNA candidates Values indicate relative expressions levels as log10 scale, with SD for ... across a pool of larvae and adult bee samples for 30 candidate miRNAs Some candidates were queried with multiple primers in order to test for strand-based expression and to distinguish between...
Ngày tải lên: 14/08/2014, 07:21
Tài liệu Cyber Forensics—A Field Manual for Collecting, Examining, and Preserving Evidence of Computer Crimes ppt
... this information are advised and encouraged to confirm specific claims for product performance as necessary and appropriate The legal/financial materials and information that are available for reference ... reporting and subsequent information is available Ask for the entire detail report and see what they record; not just ask for the basics; you might save time and effort if you ask for everything ... of Digital Evidence .134 Recovering and Examining Computer Forensic Evidence 140 International Principles for Computer Evidence 141 Chapter 9: Computer Crime Policy and Programs...
Ngày tải lên: 18/01/2014, 06:20
Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes (upper and lower) ... abbreviated as aD, bD, and cD, respectively, and the a and b subunits of the reactivase are abbreviated as aR and bR, respectively, molar ratios of aD, bD, cD, aR and bR in bands i and vi were determined ... thick bands and one thin band on the front line, irrespective of the presence of enzyme (Fig 5A, lanes b, c, e, and g) To determine their subunit compositions, bands i–vi were excised and subjected...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Scientif ic Evidence for Musculoskeletal, Bariatric, and Sports Nutrition pdf
... experience, maintaining blood pH and temperature, fueling the brain and vital organs, and defending against foreign invaders have been afforded top priority and receive minute-by-minute attention ... trademarks, and are used only for identification and explanation without intent to infringe Library of Congress Cataloging-in-Publication Data Scientific evidence for musculoskeletal, bariatric, and ... Scientif ic Evidence for Musculoskeletal, Bariatric, and Sports Nutrition This page intentionally left blank Scientif ic Evidence for Musculoskeletal, Bariatric, and Sports Nutrition...
Ngày tải lên: 15/02/2014, 16:20
Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx
... previously in TatA, B and C and we present new information on potentially important TatA and TatC mutants Results Analysis of TatA and TatB mutants The overall structures of the TatA and TatB subunits ... blocked in translocation activity We also present evidence for interactions 2262 C M L Barrett and C Robinson between the TM spans of TatA and TatB, and between the amphipathic regions Finally, we ... domains and cytoplasmic regions of TatA and TatB, and the highly conserved residues of TatC have also been probed [24–27] In this report we have analyzed the transmembrane regions of TatA and TatB,...
Ngày tải lên: 19/02/2014, 17:20
Nutrition and cancer: A review of the evidence for an anti-cancer diet pptx
... convincing evidence that a high fruit and vegetable diet would reduce cancers of the mouth and pharynx, esophagus, lung, stomach, and colon and rectum; evidence of probable risk reduction was found for ... fewer side effects for women undergoing radiation therapy for carcinomas of the uterine cervix [175], for patients undergoing radiation therapy for head and neck cancers [176], and for colorectal ... and 22 animal studies [81] They found "the evidence for a protective effect of greater vegetable and fruit consumption is consistent for cancers of the stomach, esophagus, lung, oral cavity and...
Ngày tải lên: 06/03/2014, 02:21
Credit Access for Small and Medium Firms Survey Evidence for Ireland doc
... Credit Access for Small and Medium Firms: Survey Evidence for Ireland Martina Lawless and Fergal McCann∗ Central Bank of Ireland Abstract The extension of credit to SMEs in Ireland has been identified ... risk profile and information asymmetries between the firm and lending institution (OECD, 2006) It can be difficult for SMEs to convince banks of the quality of their business plans and, for newer firms ... means of successful and unsuccessful applicants for finance for each performance measure The hypothesis being tested is that the means are equal between rejected and accepted firms For all four of...
Ngày tải lên: 06/03/2014, 08:20
Infants’ reasoning about hidden objects: evidence for event-general and event-specific expectations pdf
... Song and Suhua Wang for helpful discussions and comments; to Steve Holland, our graphic artist, for his patience and creativity in preparing our figures; to Laura Brueckner, Venessa Nolen and the ... rabbit move back and forth behind a screen (see Figure 14) Next, a window was created in the screen’s upper half, and the infants again saw the tall and the short rabbit move back and forth behind ... opaque, and so on Fourth, as infants form event categories and learn what variables to consider in each category, they include more and more of this detailed information, or variable information,...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx
... spectra were calibrated to an accuracy of cm)1 for intense isolated bands, with indene as the standard for the high-frequency region and with indene and CCl4 for the low-frequency region Redox potential ... the two bands observed for the Thr171Ser mutant, upon raising the pH, suggests that the two species are independent and not in equilibrium, as is thought to be the case for CCP [23] and Coprinus ... changes in Fe-His band frequencies and the redox potential values This is exemplied by the case of CCP and its mutants D235E, D235N and D235A [27] The H-bond between the proximal His and Asp235 is...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... and Xenopus lysates, which recognises the PR65 a and b isoforms equally well The best anti-C monoclonal was found to be F2 6A10, and it recognizes PP2AC in rabbit, mouse and Xenopus oocytes and ... for PCR 3) and the anchor primer supplied in the 5¢-RACE kit For amplification of specific XPR130 5¢-end RACE fragments, the primer 5¢-TGGACTGACAGTTGGAG TAAGG-3¢ was used for the RT reaction, and ... 0.1% (v/ v) Tween-20 and 0.5 M NaCl The glutathione–Sepharose beads were further eluted by the addition of SDS/PAGE sample buffer and boiled for Western blots were performed and developed as described...
Ngày tải lên: 08/03/2014, 08:20