box 25 6 anatomic sites and liposuction aggres­sive­ness17

... correlation between 3-CH and 4-CH2 (2.53 p.p.m and 2.19 p.p.m.), and the correlation between 3-CH and 2-CH2 (1.97 p.p.m and 1.52 p.p.m.), are clearly intact In the TOCSY spectrum (Fig 6B), all the expected ... the 16- CH2 signals have shifted to 1.80 and 2.44 p.p.m (protons) and 32.0 p.p.m (carbon), as indicated by the COSY correlation between 1.53 p.p.m (15-CH2) and 2.44 p.p.m (one proton on 16- CH2) ... collection and interpretation of the NMR data Their spectra were recorded in CDCl3 and COSY spectra lacked fine detail HSQC and expanded COSY were not provided by Guryev et al [2] and, without...

Ngày tải lên: 18/02/2014, 17:20

... exposure was overnight FEBS Journal 273 (20 06) 9 36 947 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 941 Cyp4x1 in mouse brain A M Al-Anizy et al 1 16 97 66 43 M B U P S 4x B M 10 10 Brain Liver ... 273 (20 06) 9 36 947 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 945 Cyp4x1 in mouse brain 10 11 12 13 14 15 16 M Al-Anizy et al Cyp4a and Cyp4b but not of CYP4F, genes in mouse and humans ... R534 56 and AA337301 were obtained from HGMP (Hinxton, Cambridgeshire, UK), and sequenced on both strands The latter clone was digested with EcoRI and the 1.2-kb fragment was radiolabelled with random...

Ngày tải lên: 19/02/2014, 07:20

... to CYP6A2wt pmol per pmol P450 per 30 Ratio to CYP6A2wt pmol per pmol P450 per 30 Ratio to CYP6A2wt CYP6A2wt CYP6A2vS CYP6A2vV CYP6A2vL CYP6A2vSV CYP6A2vSVL 0.03 0.04 0.05 0.01 0.04 0 .63 1.00 ... i.e CYP6A2wt, CYP6A2vS and CYP6A2vSVL, the ratio dicofol : DDA (specific activities) is 70.00, 251.50 and 28 .62 , respectively, and the ratio DDD/DDA (specific activities) is 43.00, 62 .25 and 11.90, ... (normalized values) CYP6A2wt CYP6A2vS CYP6A2vV CYP6A2vL CYP6A2vSV CYP6A2vSVL 15.2 8.3 12.9 12.8 8.7 8.1 960 390 65 0 750 245 190 1.00 0.55 0.85 0.84 0.57 0.53 1.00 0.40 0 .67 0.78 0.25 0.20 1.00...

Ngày tải lên: 19/02/2014, 12:20

... superoxo-ferrous state 1 56 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 in a reduced oxy-ferrous hemoprotein and model compounds J Am Chem Soc 125, 163 40 163 46 Leibl, W., Nitschke, ... (2000) Heme-(hydro) peroxide mediated O- and N-dealkylation A study with microperoxidase Eur J Biochem 267 , 66 7 366 78 1 86 Primus, J.L., Boersma, M.G., Mandon, D., Boeren, S., Veeger, C., Weiss, ... Brown, W.D & Snyder, H.E (1 969 ) Nonenzymatic reduction and oxidation of myoglobin and hemoglobin by nicotinamide adenine dinucleotides and avins J Biol Chem 244, 67 0 267 06 151 Adams, P.A & Berman,...

Ngày tải lên: 19/02/2014, 16:20

... Journal 2 76 (2009) 719–735 Journal compilation ª 2008 FEBS No claim to original French government works 733 CYP77A4, an epoxy fatty acid-forming enzyme 53 54 55 56 57 58 59 60 61 62 63 64 65 734 ... characterized plant fatty acid hydroxylases belong to the divergent CYP 86 clan (including CYP 86 and CYP94 families) Both the CYP71 and CYP 86 clans appear to have evolved independently within the green ... ketones and hydroperoxides) and some are cyclic compounds Extensively studied jasmonic acid and 12-oxo-phytodienoic acids represent a good illustration of these cyclic oxylipins [64 ,65 ] They...

Ngày tải lên: 07/03/2014, 03:20

... single-k )72 )66 )87 )89 )95 )113 )114 )135 )133 K298 Ka FAD E2 28 1 2 )221 ) 269 )208 )2 46 )219 )255 ) 261 )233 )251 E3 )288 )283 )310 )328 )331 )328 ) 366 )3 36 )380 31 10 10 26 31 E2 ... (1991) NADPHcytochrome P-450 oxidoreductase The role of cysteine 566 in catalysis and cofactor binding J Biol Chem 266 , 199 761 9980 26 Shen AL & Kasper CB (1995) Role of acidic residues in the interaction ... Acta 169 8, 1 26 17 Vermilion JL, Ballou DP, Massey V & Coon MJ (1981) Separate roles for FMN and FAD in catalysis by liver microsomal NADPHcytochrome P-450 reductase J Biol Chem 2 56, 266 277 18...

Ngày tải lên: 07/03/2014, 06:20

... Negative charge residues (D88 and D393) are depicted in red, haem in magenta, haem-iron in grey, and positive charge residues (K62, R64, R174, and K291) in blue Data from PDB CL6 [9] Ó FEBS 2004 2890 ... (1999) Cytochrome P450 and the individuality of species Arch Biochem Biophys 369 , 1–10 Omura, T (1999) Forty years of cytochrome P450 Biochem Biophys Res Commun 266 , 69 0 69 8 Sono, M., Roach, M.P., ... nitrate/nitrite-inducible cytochrome P-450 cDNA from Fusarium oxysporum J Biol Chem 266 , 1 063 2–1 063 7 Nakahara, K & Shoun, H (19 96) N-Terminal processing and amino acid sequences of two isoforms of nitric oxide reductase...

Ngày tải lên: 07/03/2014, 15:20

... 34. 96 34. 96 – 51.03 23.57 23.57 30 .60 30 .60 81.45 11. 36 22.88 1 .64 2.02 2.18 2.41 N/A 5 .64 N/A 2.23 2.40 1.01 1.77 1.59 0.90 N/A 1. 56 1.43 0.94 1.81 N/A 0.93 1. 56 1. 26 1.91 1.38 3.50 0.72 1.18 36. 22 ... 1.90 1 .69 1.15 N/A 2.07 1.55 1.09 1.84 N/A 0.98 1 .60 1.31 1.98 1.47 3. 56 0.78 1.24 dd 1H, J ¼ 10.2 Hz, H2a, 4 .6 Hz, H2b – dd 1H, J ¼ 4 .6 Hz, H1, 16. 1 Hz, H2b dd 1H, J ¼ 10.2 Hz, H1, 16. 1 Hz, ... 51 .65 24.1 24.1 30.2 30.2 82.2 11.4 13.25 2.31 1.48 4.11 N/A N/A 5.70 N/A 2.20 2.51 1.09 1.77 1.90 1.39 N/A 1.71 1.15 0.94 1.92 N/A 0. 96 1.51 1.27 1.88 1. 36 3.49 0.71 1.15 40. 46 40. 46 69. 06 N/A...

Ngày tải lên: 07/03/2014, 16:20

... (1998) Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae Chem Biol 5, 66 1 66 7 Rodriguez AM, Olano C, Mendez C, Hutchinson ... involve an inducible cytochrome P-450 system and aldehyde dehydrogenase J Bacteriol 177, 67 6 68 7 Trower MK, Sariaslani FS & O’Keefe DP (1989) Purification and characterization of a soybean flour-induced ... determination, and heterologous expression of the proteins J Biol Chem 265 , 60 66 60 73 24 Narhi LO & Fulco AJ (1987) Identification and characterization of two functional domains in cytochrome P-450BM-3,...

Ngày tải lên: 07/03/2014, 16:20

... (bovine: 391 pmolÆmg)1) [ 26] , corpus luteum (bovine: 250 pmolÆmg)1) [ 26] , follicles and corpus luteum (porcine: 2–11 and 78 pmolÆmg)1, respectively) [27], and placenta (human: 2 .6 pmolÆmg)1 protein) ... comparison with Fig and consisted of 85% methanol and 0.1% acetic acid from to 25 min, followed by linear gradient to 100% methanol and 0.1% acetic acid from 25 to 35 min; and 100% methanol and 0.1% acetic ... methanol and 0.1% acetic acid from to 30 min, followed by linear gradient to 100% methanol and 0.1% acetic acid from 30 to 45 min; and 100% methanol and 0.1% acetic acid from 45 to 60 The MS...

Ngày tải lên: 07/03/2014, 21:20

... (Eur J Biochem 269 ) 561 3 Fig Ratio of 17-OHPREG and DHEA formation during PREG (1 lM) metabolism by baboon and human CYP17 expressed in HEK-293 cells The Km and V values for PROG and PREG utilization ... human and baboon CYP17 lie in the predicted substrate access and binding regions which include helices F and G and the F-G loop The differences between the two species in the F and G helix and ... ÔPrinciples of Laboratory CareÕ and the ÔNIH Guide for the Care and Use of Laboratory AnimalsÕ 19 96 Adrenal glands, allocated for RNA isolation, were flash frozen and stored in liquid N2 For all...

Ngày tải lên: 08/03/2014, 09:20

... ensembles [substrate-free: at % 1940 cm)1 (% 60 %) and % 1952–1 963 cm)1 (% 40%) and camphane: at % 1941 cm)1 (% 60 %) and 1955–1 962 cm)1 (% 40%)] [6, 9] This subconformer behaviour could explain ... F-FTIRb 5 .6 20.0 20.0 26. 8 4.8 26. 8 F F F 20.0 20.0 20.0 3.0 2.95 10.0 9.8 (1939.1 cm)1) 1 .6 (52%) & 7.8 (48%) 10.4 (61 %; 1939.4 cm)1) & 49.7 (25%; 1949.2 cm)1) 55 75 150 SF F F-FTIRb 5.0 20.0 26. 8 ... divided into two 96. 5 1R-Camphor a – – )19 .6 ± 0.9 )13.2 ± 0.8 14–1515 4–1311 10.2 ± 2.1 7 .6 ± 2.0 8.4 ± 0.8 )18.2 ± 3.8 )138.8 ± 27.8 4 .6 ± 2.0 – – )10 .6 ± 1.1 )53.1 ± 21.9 35–1140 2 06 1214 208–1418...

Ngày tải lên: 08/03/2014, 23:20

... 2.0 0.25 ± 0. 06 8.3 ± 0.5 6. 9 ± 2.4 11 ± 6. 7 ± 1.7 0.0 36 ± 0.009 14 ± 5.0 ± 0.7 0.82 ± 0.12 10 ± 6 2 13 ± 3.0 ± 1.0 0.13 ± 0.04 9.0 ± 1.0 FEBS Journal 273 (20 06) 2223–2231 ª 20 06 The Authors ... assays with d0 and perdeuterated (at the carbon being oxidized) substrates and comparing the v versus S plots (Figs and 3, and Tables and 3) The patterns were very similar for 7-OMe and 7-OEt coumarin, ... P450s 1A2 [35], 2E1 [ 36] , and 1A1 [37] were expressed in Escherichia coli and purified using modifica- FEBS Journal 273 (20 06) 2223–2231 ª 20 06 The Authors Journal compilation ª 20 06 FEBS K.-H Kim et...

Ngày tải lên: 16/03/2014, 13:20

... Functional genomics of P450s Annu Rev Plant Biol 54, 62 9 66 7 16 Nelson DR (1999) Cytochrome P450 and the individuality of species Arch Biochem Biophys 369 , 1–10 17 Burger C, Rondet S, Benveniste P & ... protein was placed in two cuvettes (sample and reference) and the differential spectra were registered after several amounts of E (2, 6, 12, 16, 25 and 40 lM) had been added to the sample cuvette ... Polypodium vulgare Eur J Biochem 266 , 60 8 61 5 ´ 10 Messeguer J, Mele E, Reixach N, Irurre-Santilari J & Casas J (1998) Polypodium vulgare L (wood fern): in vitro cultures and the production of phytoecdysteroids...

Ngày tải lên: 16/03/2014, 23:20

... 3A1 -60 0 (position )62 9 to )60 8) and 3A1-700 (position ) 766 to )7 46) encompass distinct CYP3A1 promoter regions DNA (6 pmol) was endlabelled with [c-32P] dATP using T4 polynucleotide kinase, and ... sequence of the CYP3A1 cDNA, the 3A1 -60 0 to the )62 9/ )60 8 sequence and the 3A1-700 to nucleotides ) 767 /)747 (B) EMSA was performed using a radiolabeled double-stranded oligonucleotide corresponding ... Song, B.J & Hardwick, J.P (19 86) Pregnenolone 16a-carbonitrile-inducible P-450 gene family: gene conversion and differential regulation Mol Cell Biol 6, 2 969 –29 76 Kliewer, S.A., Moore, J.T., Wade,...

Ngày tải lên: 17/03/2014, 09:20

... 1 36 ± 14 119 ± 17 83 ± 1.7 100 ± 13 (SRS 6) R 368 F 81 ± 3.3 – K484M 54 ± 4.3 – 89 ± 1.4 – 2 2 30.7 ± 0 .6 13 ± 1.2 – 60 ± 3.5 48 ± 4.1 55 ± 6. 1 11 ± 0.5 11 ± 0 .6 133 ± 6. 6 1 26 ± 5.7 118 ± 6. 0 ... 2 .6 128 ± 7.5 23 ± 4.7 98 ± 6. 9 I371A 95 ± 9.9 – B helix (SRS 1) 25 ± 3.0 11.1 ± 2.2 25 ± 1.5 15 .6 ± 2.3 1 46 ± 13 1 06 ± 12 R103M 15 ± 4.7 6. 7 ± 0.7 44 ± 4.5 R103E DKR K helix (SRS 5) KRI R 366 M ... proline residues (P 360 and P 364 ) The resulting topology orients three leucine side chains toward the active site (L358, L359 and L 363 ) In P450CAM [ 46] and P450TERP [47], SRS is a b-strand partially...

Ngày tải lên: 17/03/2014, 10:20

... reaction (2) DrG (kcalặmol ) DrGa (kcalặmol)1) DrG#b (kcalặmol)1) )63 .6 )68 .3 )65 .9 )67 .9 )65 .8 )67 .6 )63 .5 )41.3 )43.0 )40 .6 )43.8 )39.5 )41.5 )40.5 29.9 32.8 31.9 51.9 55.3 54.2 51.0 a a Relative ... Untreated rat Rat DEX 16. 0 23.0 6. 8 14.0 2.35 1 .64 10.5 15.0 12.0 25.0 0. 86 0 .60 Stability study Storage and stability of M1 Table shows analysis of the samples after incubation and after day storage ... ) 36) 459 ()30) 441 ()30, )18) 429 ( )60 ) 399 ( )60 , )30) 3 56 ()57) 413 M + H ) H2O C2H3NO 342 ()71) Ala 217 ()1 96) (NMe-DPhe-Gly)+ 431 328 ()103) M+H C3H5NO3 318()113)-Leu 453 423 ()30) M + Na 469 ...

Ngày tải lên: 22/03/2014, 16:20

... suberin and seed coat, showing that production of C 16 and C18 x-hydroxy FA by other hydroxylases was sufficient to maintain this major property of polyester-based barriers Although CYP86A1 and CYP86B1 ... CYP86A4 The sequential order of the hydroxylation reactions was demonstrated by both cutin monomer profiles in knockout mutants for CYP77A6 and CYP86A4 and the fact that recombinant CYP77A6 expressed ... of CYP86B1 [ 26] This mutation resulted in a strong reduction of C22 and C24 x-hydroxy saturated FA derivatives in root suberin and seed coat polyesters Surprisingly, downregulation of CYP86B1 did...

Ngày tải lên: 22/03/2014, 16:21

... hexadecanoic acid, 1,16hexadecandiol, 16- hydroxyhexadecanoic acid and even 1, 16- hexadecanedioic acid, in this way bypassing the two enzymes normally involved in x-oxidation [ 26] The authors did ... unsaturated C 16 and C20 fatty acids as well, and was assigned as CYP6001A1 [53] Several CYP6001 homologues can be retrieved among fungi such as other Aspergillus sp., Neurospora sp., Fusarium sp and Ustilago ... endoplasmicreticulum Biochemistry 30, 71– 76 60 Nebert DW & Gonzalez FJ (1987) P450 genes: structure, evolution, and regulation Annu Rev Biochem 56, 945–993 61 Narhi LO & Fulco AJ (19 86) Characterization of a...

Ngày tải lên: 22/03/2014, 16:21