box 25 4 commonly used sedatives and analgesics15
Tài liệu Báo cáo khoa học: Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc docx
... cytochrome P450 enzymes Chem Rev 1 04, 3 947 –3980 Tuckey RC & Stevenson PM (19 84) Properties of ferredoxin reductase and ferredoxin from the bovine corpus luteum Int J Biochem 16, 48 9 49 5 Tuckey ... spectrum (Fig 6A), the correlation between 3-CH and 4- CH2 (2.53 p.p.m and 2.19 p.p.m.), and the correlation between 3-CH and 2-CH2 (1.97 p.p.m and 1.52 p.p.m.), are clearly intact In the TOCSY ... Cancer Res 1 64, 3–28 Holick MF (2003) Vitamin D: a millenium perspective J Cell Biochem 88, 296–307 Bikle DD (20 04) Vitamin D regulated keratinocyte differentiation J Cell Biochem 92, 43 6 44 4 10 Zbytek...
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Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc
... of rabbit CYP4B1 (cyp4b1), rat CYP4B1 (cyp4b1) and human CYP4B1 (cyp4b1) The conserved region is underlined (B) Alignment of deduced amino acid sequence of rat, human and mouse CYP4x1 proteins ... Cyp4a and Cyp4b but not of CYP4F, genes in mouse and humans Biochem J 325, 741 – 749 Jeffery B et al (20 04) Peroxisome proliferator activated receptor alpha regulates a male-specific cytochrome P450 ... novel murine P -45 0 gene, Cyp4a 14, is part of a cluster of FEBS Journal 273 (2006) 936– 947 ª 2006 The Authors Journal compilation ª 2006 FEBS 945 Cyp4x1 in mouse brain 10 11 12 13 14 15 16 M Al-Anizy...
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Tài liệu Báo cáo khóa học: Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism doc
... H117 and F4 84) and two in substrate specificity (F116 and F4 84) This was achieved after sequence alignment analyses and site-directed mutagenesis [32] These amino acids belong to the SRS1 and SRS6 ... structure of human cytochrome P450 2C9 with bound warfarin Nature 42 4, 46 4 46 8 24 de Groot, M.J & Ekins, S (2002) Pharmacophore modeling of cytochromes P450 Adv Drug Deliv Rev 54, 367–383 25 Lewis, D.F ... 12.9 12.8 8.7 8.1 960 390 650 750 245 190 1.00 0.55 0.85 0. 84 0.57 0.53 1.00 0 .40 0.67 0.78 0.25 0.20 1.00 0. 74 0.79 0.92 0 .44 0.37 ± ± ± ± ± ± 3.6 5.5 4. 6 3.8 4. 2 6.7 (3) (3) (3) (3) (3) (3) ±...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
... {4TS1-III} 2 .46 5 {2.523} OCC: 90.5 {85.0} 4TS2-IV {4TS2-III} TS1-III 17 .4 14. 9 13.9 4TS1 42 (z2) cat 4TS1-IV 42 (xy) cat 22 cat 4TS2-III 10 .4 42-III 0.9 0.6 0.0 4, 21 + C2H4 22-IV 4TS2-IV 3.2 42 -IV ... carbon 43 52 P Hlavica (Eur J Biochem 271) 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 monoxide on the N-oxidation of N,N-dimethylaniline HoppeSeyler Z Physiol Chem 355, 150815 14 Gasser, ... 0.0 4, 21 + C2H4 22-IV 4TS2-IV 3.2 42 -IV 1 .48 6 (1 .48 2) {1 .48 8} [1 .46 5] 1.810 (1.808) {1.821} [1.978] 2.9 1 .46 4 (1 .47 3) {1 .46 9} [1.395] 2.381 (2 .42 1) {2 .44 7} [2.362] OCC: 107.0 (108.2) {107.7} [119.5]...
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Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx
... cytochrome P450 arachidonic acid epoxygenase indicates that 14, 15epoxyeicosatrienoic acid functions as an intracellular CYP77A4, an epoxy fatty acid-forming enzyme 41 42 43 44 45 46 47 48 49 50 51 ... long chain fatty acids by recombinant CYP4F8 and CYP4F12 and catalytic importance of Tyr-125 and Gly-328 of CYP4F8 Arch Biochem Biophys 44 1, 1 74 181 19 Kandel S, Sauveplane V, Olry A, Diss L, Benveniste ... associated with angiogenesis [41 ,42 ] CYP2C and CYP2J2 have also been shown to be expressed in different tumor tissues [43 ,44 ] Epoxides of fatty acids are less described in plants, and only a few biological...
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Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
... E2 [FMNhqFADox] [FMNsqFADsq] E4 5 3 11 )388 )382 )40 3 )381 ) 342 )41 7 )385 )46 2 )41 9 11 10 6 11 1.7 103 23.7 75.6 16.8 57.7 53 .4 145 .8 1 .4 2.7 0.3 0.2 0.3 0.2 0.7 0.2 ... cytochrome P450 reductase FEBS Journal 275 (2008) 45 4 045 57 ê 2008 The Authors Journal compilation ê 2008 FEBS 45 41 Electron transfer in cytochrome P450 reductase S Brenner et al CPR [30]) and the ... namely )43 mVặpH)1 (E1), )17 18 mVặpH)1 (E2), )32 mVặpH)1 (E3) and )47 10 mVặpH)1 (E4) The incomplete expression of the expected redoxBohr effect may result from FEBS Journal 275 (2008) 45 4 045 57...
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Báo cáo khoa học: D88A mutant of cytochrome P450nor provides kinetic evidence for direct complex formation with electron donor NADH ppt
... same buffer and eluted with a 0–0 .4 M KCl gradient The P450nor fraction was concentrated, dialysed, and stored at °C until further analyses Ó FEBS 20 04 Asp88 in haem-distal pocket of P450nor (Eur ... as those of the wild-type P450nor, i.e its ferric resting form (Fe3+) comprises a mixture of high- and low-spin states, and the CO-bound form gives a Soret band at 44 8 nm These spectra show that ... haem-distal pocket of P450nor Negative charge residues (D88 and D393) are depicted in red, haem in magenta, haem-iron in grey, and positive charge residues (K62, R 64, R1 74, and K291) in blue Data...
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Báo cáo khoa học: Testosterone 1b-hydroxylation by human cytochrome P450 3A4 pdf
... 1.15 40 .46 40 .46 69.06 N/A – 119.33 – 33.18 33.18 37.08 37.08 30.55 50.90 – 22.89 22.89 34. 96 34. 96 – 51.03 23.57 23.57 30.60 30.60 81 .45 11.36 22.88 1. 64 2.02 2.18 2 .41 N/A 5. 64 N/A 2.23 2 .40 ... 55.65 – 24. 20 24. 20 37.8 37.8 – 51.65 24. 1 24. 1 30.2 30.2 82.2 11 .4 13.25 2.31 1 .48 4. 11 N/A N/A 5.70 N/A 2.20 2.51 1.09 1.77 1.90 1.39 N/A 1.71 1.15 0. 94 1.92 N/A 0.96 1.51 1.27 1.88 1.36 3 .49 0.71 ... 1 .43 0. 94 1.81 N/A 0.93 1.56 1.26 1.91 1.38 3.50 0.72 1.18 36.22 36.22 34. 33 34. 33 – 123. 84 – 33.12 33.12 37.17 37.17 36.19 54. 76 – 21.30 21.30 32.32 32.32 – 51.09 23.78 23.78 30 .48 30 .48 81.54...
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Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt
... 56-kDa CYP153 from FEBS Journal 272 (2005) 1 148 –1159 ª 2005 FEBS Studies on the mycobacterial P450mor system 41 42 43 44 45 46 47 48 49 Acinetobacter sp EB1 04 Biochem Biophys Res Commun 286, 652–658 ... Biochemistry 30, 44 7– 45 5 26 Peterson JA, Lu JY, Geisselsoder J, Graham-Lorence S, Carmona C, Witney F & Lorence MC (1992) Cytochrome P -45 0terp Isolation and purification of the protein and cloning and sequencing ... bacterial P450 system distinct from other purified bacterial P450 systems which either utilize a Fe2S2 ferredoxin-like P450cam [23] or belong to the microsomal type of P450s like P450BM3 [ 24] and are...
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Báo cáo khoa học: The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism docx
... hydroxylates vitamin D3 31 32 33 34 35 36 37 38 39 40 41 42 43 epithelial cells synthesize glucocorticoids and regulate T cell activation J Exp Med 200, 1635–1 646 Dalla Valle L, Toffolo V, Vianello ... adrenals [41 ,42 ] Adrenodoxin (FDX1) was expressed in E coli and purified as described previously [43 ] Reactions to modify the side chain of vitamin D3 were performed with purified bovine P450scc and ... 40 °C The mobile phases consisted of 85% methanol and 0.1% acetic acid from to 30 min, followed by linear gradient to 100% methanol and 0.1% acetic acid from 30 to 45 min; and 100% methanol and...
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... porcine and hamster CYP17 catalyse the D4 and D5 hydroxylase and lyase reactions, yielding both DHEA and A4 [ 14 16] The alignment of mammalian cytochromes P450 with bacterial cytochromes P450 has ... with a maximum at 42 4 nm and a minimum at 40 9 nm is characteristic of cytochrome b5 [20] The concentration of baboon adrenal cytochrome P450 was 0.55 nmolÆmg)1 microsomal protein and the concentration ... microsomes (0.5 lM P450) and 15 min, respectively, are shown in Fig 3A and B A4 and 16a-hydroxyprogesterone were not detected The metabolism of 17-OHPROG by baboon adrenal microsomes (Fig 4) showed that...
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Báo cáo Y học: Substrates modulate the rate-determining step for CO binding in cytochrome P450cam (CYP101) A high-pressure stopped-flow study pdf
... 0.8 14 1515 4 1311 10.2 ± 2.1 7.6 ± 2.0 8 .4 ± 0.8 )18.2 ± 3.8 )138.8 ± 27.8 4. 6 ± 2.0 – – )10.6 ± 1.1 )53.1 ± 21.9 35–1 140 206–12 14 208– 141 8 11–1150 1150– 149 0 5.6 20.0 50 40 0 2.80 3.05 2. 94 3.18 ... 2.68 – 40 00 2000 40 0 20 10 50 5.0 4. 5 3.8 4. 8 1 1 1150 1380 1 5.1 41 .1 69.9 91.8 Substrate-free Norcamphor Norbornane Camphane 29.50 ± 0.70 (46 %) 297 ± ( 54% ) – 381 ± 43 (100%) – 332 ± 14 (100%) ... kJÆmol)1 and )27 .45 kJÆmol)1 for camphor-bound # and substrate-free P450cam, respectively Therefore, DGon )1 for substrate-free P450cam is lower ( 34. 44 kJÆmol ) than # DGon for camphor-bound P450cam...
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Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf
... P450 1A2 and an excess of NADPH-P450 reductase (BD Gentest SupersomesÒ, 20 pmol P450 in 0.50 mL of 50 mm potassium phosphate buffer, pH 7 .4) were used instead of the recombinant (bacterial) P450 ... 4. 2 ± 1.0 7-OMe coumarinc 9 .4 ± 0 .4 9.6 ± 0.2 7-OEt coumarind 6.1 ± 1 .4 6.1 ± 1.1 a A : molar mixture of d0 and [methyl-d3] 7-OMe coumarin was used as the substrate, and the kinetic isotope effect ... assays with d0 and perdeuterated (at the carbon being oxidized) substrates and comparing the v versus S plots (Figs and 3, and Tables and 3) The patterns were very similar for 7-OMe and 7-OEt coumarin,...
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Báo cáo khoa học: The first cytochrome P450 in ferns Evidence for its involvement in phytoecdysteroid biosynthesis in Polypodium vulgare doc
... for P450 induction, and the amount of E (inducing P450 protein) and 20E (inhibiting P450 activity) present in the tissues at this time When E is topically applied to calli, P450 is expressed and ... and incubated for 24 h Microsomes were prepared and their P450 content was measured (B) We applied 60 nmol E per 100 mg to the calli and P450 was measured at different times P450 content was measured ... calli Control calli, and P450 inhibitortreated calli, were treated with E and after 24 h the transformation of E to 20E was evaluated using HPLC As shown in Fig 4, all the P450 inhibitors D Canals...
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Báo cáo khoa học: Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response ppt
... genes 10 11 12 13 14 15 Acknowledgements Supported by Fundacao para a Ciencia e Tecnologia – grant PRAXIS ¸ ˜ ˆ 4/ 4.1/BD/2999 (E.R); project PRAXIS XXI 2/2.1/BIA/2 34/ 94 – and by the Franco-Portuguese ... rapid (30 min) increase of C/EBPa and C/EBPb mRNA was observed [42 ] Others have shown that induction of C/EBPb mRNA by dexamethasone begins 30 after treatment [43 ,44 ] Dexamethasone transiently reduces ... lg of CYP3A1T81Luc plasmid and different amounts of each expression vector Cells were inoculated in 24- well plates and maintained for 48 h These cells were harvested and lysed in reporter lysis...
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Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt
... Struct Biol 3, 41 4 41 7 Schlichting, I., Berendzen, J., Chu, K., Stock, A.M., Maves, S.A., Benson, D.E., Sweet, R.M., Ringe, D., Petsko, G.A & Sligar, 35 36 37 38 39 40 41 42 43 44 45 46 47 S.G (2000) ... residues (P360 and P3 64) The resulting topology orients three leucine side chains toward the active site (L358, L359 and L363) In P450CAM [46 ] and P450TERP [47 ], SRS is a b-strand partially involved ... 3685 Table PCR primers used for site-directed mutagenesis The DKR primer, meant to generate K 248 T/R 249 M double mutants, actually produced the D 247 E/K 248 T/R 249 M and K 248 T/R 249 M/I371K triple mutants...
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Báo cáo khoa học: Metabolism of N-methyl-amide by cytochrome P450s Formation and characterization of highly stable carbinol-amide intermediate pdf
... (NMe-DPhe-Gly)+ 43 1 328 ()103) M+H C3H5NO3 318()113)-Leu 45 3 42 3 ()30) M + Na 46 9 43 9 ()30) M+K 42 9 M)H 46 5, 46 7 M ) H + HCl 48 9 M)H+ CH3CO2H MS-MS+ CH2O and H2O), 42 9 (loss of AcOH) and 399 (loss of AcOH and ... 355 ( )44 ) CH2NO 289 ()110) 271 ()128) 41 1 ()18) 381 ()30, )18) 311 ()118) 43 5, 43 7 ()30) 399 ()30, )36) 45 9 ()30) 44 1 ()30, )18) 42 9 ()60) 399 ()60, )30) 356 ()57) 41 3 M + H ) H2O C2H3NO 342 ()71) ... 3B and Table 2), the mass spectrum shows fragments at 41 3, 43 1, 45 3 and 46 9 Both m z at 45 3 (M + Na)+ and m z at 46 9 (M + K)+ conducted under collision lead to 30 amu losses, whereas the 41 3...
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Báo cáo khoa học: Cytochrome P450 metabolizing fatty acids in plants: characterization and physiological roles pptx
... acids and is required for anther cutin biosynthesis FEBS Journal 278 (2011) 195–205 ª 2010 The Authors Journal compilation ª 2010 FEBS F Pinot and F Beisson 40 41 42 43 44 45 46 47 48 49 and pollen ... is also conserved in land plants and typically each plant species contains only one CYP703 [37] CYP704B1 and CYP704B2 belong to a conserved and ancient P450 family in moss and seed plants This ... vitro activity and physiological roles of characterized plant cytochrome P450s metabolizing fatty acids Cytochrome P450 Plant Enzymatic activity Physiological role Ref 94A1 94A2 94A5 94C1 86A1 86A2...
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Báo cáo khoa học: The role of cytochrome P450 monooxygenases in microbial fatty acid metabolism pdf
... microbial fatty acid metabolism 34 35 36 37 38 39 40 41 42 43 44 45 46 hydrogen peroxide dependent fatty acid alpha-hydroxylase from Sphingomonas paucimobilis J Biochem 1 24, 105–110 Sono M, Roach MP, ... Schunck W-H (1996) Characterization of the n-alkane and fatty acid hydroxylating cytochrome P450 forms 52A3 and 52A4 Arch Biochem Biophys 328, 245 –2 54 24 Zimmer T, Scheller U, Takagi M & Schunck W-H ... microbial fatty acid metabolism 47 48 49 50 51 52 53 54 55 56 57 58 59 220 I N A Van Bogaert et al sophorolipid-producing yeast Candida bombicola FEMS Yeast Res 9, 87– 94 Alexander NJ, Proctor RH & McCormick...
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Báo cáo khoa học: An alternative pathway of vitamin D2metabolism Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2and 17,20-dihydroxyvitamin D2 pot
... 70 65 60 55 50 45 361.0 40 35 30 M-H2O 3 94. 0 25 20 15 Vit D2 P M2 2.6E5 2.1E5 1.7E5 1.3E5 333.0 M 10 8.6E4 4. 3E4 41 2.0 351.0 0.0E0 M1 6.4E5 6.0E5 5.6E5 5.2E5 4. 7E5 4. 3E5 3.9E5 3.4E5 3.0E5 M-2H2O-CH3 ... molecular ion of metabolite had m ⁄ z ¼ 42 8, with fragment ions at m ⁄ z ¼ 41 0 (42 8—H2O), m ⁄ z ¼ 392 (42 8—2H2O), m ⁄ z ¼ 395 (41 0—CH3) and m ⁄ z ¼ 377 (42 8—2H2O–CH3) Since vitamin D2 has m ⁄ ... (kmax at 265 nm and kmin at 228 nm) For metabolite 1, the molecular ion had m ⁄ z ¼ 41 2 with fragment ions m ⁄ z ¼ 3 94 (41 2—H2O), m ⁄ z ¼ 379 (3 94 CH3), m ⁄ z ¼ 376 (41 2—2H2O) and m ⁄ z ¼ 361...
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