body your cells eukaryotic cells

... membrane No sterols Sterols Ribosomes 70S 80S Cytoskeleton Absent present PROKARYOTIC CELLS Key Functions of Cells A boundary that keeps the cellular contents separate from the external environment ... for movement •a hook where the filament attaches •a basal body that anchors the hook to the cell •the arrangement of the hook/basal body articulation allows the hook with its filament to rotate ... protoplasm of the prokaryotic cell: chromatin body or the bacterial chromosome nucleoid or nuclear region of the cell that is associated with the chromatin body plasmids are tiny circular extrachromosomal...

Ngày tải lên: 13/03/2014, 19:27

... cells have cell membranes (outer covering of the cell) Both types of cells have ribosomes Both types of cells have DNA Both types of cells have a liquid environment known as the cytoplasm  Your ... living cells The Cell Theory All Organisms are composed of one or more cells The cell is the basic unit of structure and organization of organisms All cells come from other living cells Cells ... level of organization: cells What is the first thing that we need to know about cells?  All cells fall into one of the two major classifications of either prokaryotic or eukaryotic What’s the...

Ngày tải lên: 13/03/2014, 19:31

... COS-7 cells After 24 h, cells were fixed and detected by direct fluorescence Merged images demonstrating co-localization of proteins (yellow) are shown on the right Scale bar = 25 lm (B) COS-7 cells ... LRH-1 subnuclear localization in rat granulosa cells EGFP-LRH1 wild-type was cotransfected with DsRed-SUMO-1 into primary rat granulosa cells After 24 h, cells were treated with dimethylsulfoxide ... localization in rat granulosa cells EGFP-LRH1 wild-type (A) or multiple lysine mutants (B–F) were cotransfected with DsRed-SUMO-1 into primary rat granulosa cells After 24 h, cells were fixed and detected...

Ngày tải lên: 23/03/2014, 06:20

... observed in HEK 293 T cells after transfection, whereas transformation of CEdG-modified plasmids into E coli cells showed only a slight cytotoxic effect Hence, eukaryotic cells may have developed ... when a sufficient number of cells was analyzed (Fig 2B) In order to investigate whether CEdG has the potential to accumulate in genomic DNA of nongrowing cells, HSC-T6 cells were kept in minimal ... used The growth medium was removed from the confluent cells The cells were washed twice with NaCl ⁄ Pi for cell culture (Biochrom) Half of the cells were harvested immediately, combined, washed...

Ngày tải lên: 30/03/2014, 04:20

... RNA of infected cells (B) Detection of dengue E protein with an anti-E monoclonal antibody via Western blot analysis in infected cells (C) Growth of the Den-2 virus in C6/36 cells with or without ... in C6/36 cells, the cell death rates were 2.15% and 2.12%, respectively; the rates did not evidently change even when cells were treated with CPO (1.14% and 9.85%, respectively) When cells were ... virus in infected cells treated with CPO compared to non-treated cells did not significantly differ This indicated that induction of eIF5A may just prolong the survival of infected cells, providing...

Ngày tải lên: 12/08/2014, 01:21

... progenitor cells (CD34+/CD45- cells) These cells are thought to be early precursors of endothelial progenitor cells and function to replenish aging as well as damaged endothelial cells that line ... progenitor cells Our decision to considered CD34 positive/CD45 negative circulating cells as “circulating EPCs” was based on the work [28], in which blood–derived cells from which endothelial cells ... percentage of cells after the diet to the percentage of cells before the diet) and the percentage of body fat lost by each subject during the study A correlation also exists between CD34+ cells and...

Ngày tải lên: 25/10/2012, 10:51

... For comparison cells were transfected with pH2A-KillerRed whose histone fusion partner H2A acts as a structural protein, a crucial component of the chromosomal chromatin In the cells the histone ... could demonstrate that a very close distance of KillerRed to DNA did not allow the cells to survive The investigated cells were grown and treated in cell culture, but illumination and the following ... cancer cells, first characterized and documented by the Stone group [19], were cultivated and maintained in RPMI1640 (Gibco 11825) amended with FCS (2%) (Gibco) For experiments subconfluent cells...

Ngày tải lên: 25/10/2012, 11:15

... subjected to SDS–PAGE, and bands were captured Treatments of AGS cells AGS cells in the logarithmic phase were divided into two groups Cells in one group were grown in the medium containing μmol/L ... 24 h Detection of ROS using Flow cytometry Cells were washed with PBS once After trypsin digestion, AGS cells were re-suspended in PBS and 1×104 viable cells were measured in each sample by FACScalibur ... AGS cells after24 h of stimulation by Hp extracts of various concentrations a: Negative control Cells were stimulated by 960 µg/ml E coli extract and the ROS level was 3; b: Positive control Cells...

Ngày tải lên: 25/10/2012, 11:18

... molecules were involved Once tissues were ischemic, stem cells in circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites ... of stem cells, it is very important to observe the survival and curative effects of transplanted stem cells Traditional immunohistochemical method can easily identify the transplanted cells with ... stromal progenitor cells (mesenchymal progenitor cells) : implications for therapeutic use Bone Marrow Transplant,1995,16(4):557-564 Aggarwal S, Pittenger MF Human mesenchymal stem cells modulate...

Ngày tải lên: 25/10/2012, 11:18

... growing body of evidence suggests that many cell types present in asthmatic airways, such as T cells, platelets, macrophages, endothelial cells, fibroblasts, epithelial cells, and mast cells, ... chemokine that has been shown to be a potent chemoattractant for T cells, eosinophils, basophils, monocyte/macrophages, and mast cells [22] It has been shown that RANTES induces recruitment of eosinophils ... Hizawa et al [14] suggested that mononuclear cells from subjects who carried the -28G allele produced significantly greater levels of RANTES protein than cells from subjects who did not carry this...

Ngày tải lên: 26/10/2012, 09:39

... realizing high local TMZ concentrations in the nuclei of target cells To eradicate the target cells, without considering the HRPC cells resistance against therapeutic interventions it is necessary ... DU 145 prostate cancer cells after treatment with TMZ alone and with its TMZ-BioShuttle derivative Control DU 145 cells form a continuous monolayer, while treated DU 145 cells show loss of adhesion ... treated cells combined with spread and attached to the well-plate Whereas in the TMZ treated cells, the cell 342 closeness was declined and accompanied with an increase of amount of dead cells...

Ngày tải lên: 26/10/2012, 09:48

Ngày tải lên: 26/10/2012, 09:48

... of exposure to hypoxia or normoxia, cells (1×106 cells/ flask) were collected by trypsinisation Cell lysates of hypoxia-treated cells and untreated control cells were assayed for phosphorylated ... anti-JNK 1&2 antibody for hours Then an anti-phospho-JNK 1&2 (pThr183/pTyr185) rabbit antibody was added, and incubated for hour After washing the wells, an anti-rabbit IgG-HRP antibody was added, ... JNK1&2 (-fold of control) = absorbance of hypoxia-treated cells/ absorbance of control cells RT-PCR analysis Total RNA was extracted from JAR cells using RNeasy mini kits (QIAGEN, Mississauga, Canada)...

Ngày tải lên: 31/10/2012, 15:12

... the conserved gene expression profile in confluent HepG2 cells in vitro [21], the present study was performed only using HepG2 cells HepG2 cells were seeded in 250 ml tissue culture flasks (Falcon, ... standard deviation (SD) Results from HepG2 cells treated with 1mM concentration of ethanol (group 1) were compared to non-treated HepG2 cells (control cells, group 2) using Student's t-test Statistical ... hierarchical gene expression profile of mM ethanol concentration treated HepG2 cells (group 1) and control cells (HepG2 cells without treatment; group 2) exposed for a h period Data are presented...

Ngày tải lên: 31/10/2012, 15:28

... values ± standard deviation (SD) Results from HepG2 cells treated with 1mM concentration of ethanol were compared to non-treated HepG2 cells (control cells) using Student's t-test Statistical significance ... atmosphere of 7.5% CO2 After days of cell culture, the cells were harvested with 0.05% trypsin / 0.02% EDTA (Gibco BRL, Eggersheim, Germany) Cells were seeded in 6-well plates (Falcon-Becton Dickinson, ... experiments were performed (n=6) Each set consist of two groups as follow: HepG2 cells treated with mM ethanol and HepG2 cells without ethanol treatment as a control The rationale for using a low concentration...

Ngày tải lên: 31/10/2012, 15:37

Ngày tải lên: 31/10/2012, 16:49

... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells ... so as to enable some of the cells to undergo mitosis Mitotic hESC were then arrested at metaphase by colcemid treatment (0.1 μg/ml) for 16-18 h Subsequently, the cells were subjected to hypotonic ... (3×), the cells were incubated with a mixture of diluted primary antibodies against SSEA-3 (mouse IgM, 10 μg/ml) and TRA-1-81 (mouse IgG, 10 μg/ml) for h at room temperature The antibody mixture...

Ngày tải lên: 31/10/2012, 16:57

... fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* n.d.* FACS 6300000±1520 n.d.* n.d.* blood ... correspond to < 100 cells/ µL Human erythrocyte lack a nucleus and are thought to be void of protein synthesis In contrast, we have found that total RNA from human RBCs resembles typical eukaryotic RNA ... synthesis in human platelets by megakaryocyte-derived mRNAs 19 The finding of RNA in anucleate cells like erythrocytes support the idea of nucleus independent protein synthesis and supports data...

Ngày tải lên: 03/11/2012, 10:58

... nucleus of melanoma cells, so cells were stained with an antibody to pan-PO4-PKC Confocal microscopy demonstrates that active PKC can be found in the nucleus of G361 melanoma cells D) To determine ... the PKC substrate antibody G361 cells, which have very little endogenous claudin-1, and low levels of PKC, show no immunoprecipitation of claudin-1 with the PKC substrate antibody (Figure 1D) These ... expression, we transfected pDsRedCLDN1-NLS into G361 cells, and then treated the transfected cells with phorbol ester (PMA) Treatment of cells with 200nM PMA resulted in an almost complete exclusion...

Ngày tải lên: 03/11/2012, 11:17

... The TPs were applied to × 106 cells per probe We added 0.1 µl TP (1 mg/ ml) diluted to 100 µl medium (without FCS) to the HeLa cells in ml medium As a control, cells were transfected using HiPerfect ... Measurements HeLa cells were stably transfected with a GFP mutant (D2-GFP [BD, Clontech]) with hours half life The D2-GFP cells were used to determine the relative amount GFP-expression in cells by spectroscopic ... spectroscopic fluorescence measurements These HeLa D2-GFP cells were trypsinized after treatment and adjusted to a cell number of million cells per ml with Hank´s solution In each single experiment...

Ngày tải lên: 03/11/2012, 11:44