bleeding and thrombosis in acute promyelocytic leukemia

... Aur-A and induces monopolar spindle in NB4-R2 cells. (A) VX-680 inhibited phosphorylation of Aur- A at Thr288 in NB4-R2 cell line. Cells were incubated with increasing doses of VX-680 for 24 hr and ... both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342. Annexin V/PI staining showed that percentage of apoptosis were 3.66%, 5.52%, 15.83%, 24.43% respectively for 24 hr, and ... with prognosis and advanced clinical stage in h ead and neck squamous cell carcinoma [14,15]. Recently study showed that Aur-A kinase was highly expressed in acute myeloid leukemia (AML) patients and suppression...

Ngày tải lên: 18/06/2014, 19:20

... VX-680 inhibits activation of Aur-A and induces monopolar spindle in NB4-R2 cells. (A) VX-680 inhibited phosphorylation of Aur- A at Thr288 in NB4-R2 cell line. Cells were incubated with increasing ... abnormal monopolar spindles, suggesting that the inhibitio n of Aurora kinase activity induced defects of mitotic spindle in VX-680 treated cells. VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 ... Li 2,4 , Dong-Jun Lin 3,4 and Quentin Liu 1,3,4* Abstract Background: Aurora kinase ensures accurate chromosome segregation during cell cycle, maintaining genetic integrity in cell division. VX-680,...

Ngày tải lên: 20/06/2014, 03:20

Ngày tải lên: 10/08/2014, 22:20

... considered independently of its ED action and might help in the understanding of the adverse effects caused in humans. Abbreviations AKT: RAC-alpha serine/threonine-protein kinase; AML: Acute Myeloid Leukemia; ... FLIP: FLICE Inhibitor Protein; TRAIL: TNF Related Apoptosis Inducing Ligand; RA: Retinoic Acid; RB: Retinoblastoma. Competing interests The authors declare that they have no competing interests. Authors' ... plastic and epoxy resins, which can be used in impact-resistant safety equipment and baby bottles, as protective coatings inside metal food containers, and as composites and sealants in dentistry....

Ngày tải lên: 18/06/2014, 15:20

... Chapter 059. Bleeding and Thrombosis (Part 1) Harrison's Internal Medicine > Chapter 59. Bleeding and Thrombosis Bleeding and Thrombosis: Introduction The human hemostatic ... normally inactive GpIIb/IIIa receptor into an active receptor, enabling binding to fibrinogen and vWF. Because the surface of each platelet has about 50,000 GpIIb/IIIa fibrinogen binding sites, ... Fibrin Clot Formation Plasma coagulation proteins (clotting factors) normally circulate in plasma in their inactive forms. The sequence of coagulation protein reactions that culminate in the...

Ngày tải lên: 07/07/2014, 01:20

... Fibrin formation and dissolution. A. Fibrinogen is a trinodular structure consisting of 2 D domains and 1 E domain. Thrombin activation results in an ordered lateral assembly ... associations. FXIIIa cross-links the D domains on adjacent molecules (C). Fibrin and fibrinogen (not shown) lysis by plasmin occurs at discrete sites and results in intermediary fibrin(ogen) degradation ... Thrombin also activates factor XIII (fibrin-stabilizing factor) to factor XIIIa, which covalently cross-links and thereby stabilizes the fibrin clot. Figure 59-2 Chapter 059. Bleeding and Thrombosis...

Ngày tải lên: 07/07/2014, 01:20

... PAI1 and PAI2, inhibit the physiologic plasminogen activators; and (2) α 2 antiplasmin inhibits plasmin. PAI1 is the primary inhibitor of tPA and uPA in plasma. α 2 antiplasmin is the main inhibitor ... of antithrombin. The rate of formation of these inactivating complexes increases by a factor of several thousand in the presence of heparin. Antithrombin inactivation of thrombin and other activated ... fibrin. In response, the endogenous fibrinolytic system is then activated to dispose of intravascular fibrin and thereby maintain or reestablish the patency of the circulation. Just as thrombin...

Ngày tải lên: 07/07/2014, 01:20

... contributed to the bleeding or thrombotic state. In addition, the history can give clues as to the etiology by determining (1) the bleeding (mucosal and/ or joint) or thrombosis (arterial and/ or venous) ... Chapter 059. Bleeding and Thrombosis (Part 4) Approach to the Patient: Bleeding and Thrombosis Clinical Presentation Disorders of hemostasis may be either inherited or acquired. ... Bleeding A history of bleeding is the most important predictor of bleeding risk. In evaluating a patient for a bleeding disorder, a history of at-risk situations, including the response to past...

Ngày tải lên: 07/07/2014, 01:20

... Chapter 059. Bleeding and Thrombosis (Part 5) Epistaxis is a common symptom, particularly in children and in dry climates, and may not reflect an underlying bleeding disorder. However, ... mild bleeding disorder such as vWD. All NSAIDs, however, can precipitate gastrointestinal bleeding, which may be more severe in patients with underlying bleeding disorders. The aspirin effect ... factor XI deficiency and in symptomatic carriers of hemophilia A. Women with underlying bleeding disorders are more likely to have other bleeding symptoms, including bleeding after dental extractions,...

Ngày tải lên: 07/07/2014, 01:20

... Coexisting coagulopathies, as seen in liver failure or disseminated coagulation; infection; platelet-inhibitory drugs; and underlying medical conditions can all increase the risk of bleeding in ... risk is inherited or acquired. Note: APC, activated protein C; TAFI, thrombin-activatable fibrinolysis inhibitor; TFPI, tissue factor pathway inhibitor. Chapter 059. Bleeding and Thrombosis ... patient with a bleeding tendency must therefore include a thorough assessment for evidence of underlying disease. Bruising or mucosal bleeding may be the presenting complaint in liver disease,...

Ngày tải lên: 07/07/2014, 01:20

... prospectively to determine its utility in predicting bleeding risk, although such studies are underway. For routine preoperative and preprocedure testing, an abnormal prothrombin time (PT) may detect ... Venous thrombosis: A multicausal disease. Lancet 353:1167, 1999.] Laboratory Evaluation Careful history taking and clinical examination are essential components in the assessment of bleeding and ... coagulation assays, proper sample acquisition and handling is critical to obtaining valid results. In patients with abnormal coagulation assays who have no bleeding history, repeat studies with attention...

Ngày tải lên: 07/07/2014, 01:20

... Vitamin K deficiency – late Direct thrombin inhibitors Prolonged thrombin time Heparin or heparin-like inhibitors Mild or no bleeding – dysfibrinogenemia Frequent, severe bleeding – ... the bleeding risk of a mildly elevated INR in this setting. The aPTT assesses the intrinsic and common coagulation pathways, factors XI, IX, VIII, X, V, II, fibrinogen, and also prekallikrein, ... formation) and coagulation test abnormalities is shown in Table 59-4. Chapter 059. Bleeding and Thrombosis (Part 8) Screening Assays The most commonly used screening tests are the PT, aPTT, and...

Ngày tải lên: 07/07/2014, 01:20

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