bisulfite modification and detection of methylation
Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... activity in both human and mouse skin [16,19] These studies have provided new insight into the substrate specificity of Tgases and have expanded the range of application of the enzyme reaction ... localization of activated TGase 3, which was found to display a pattern distinct from that of TGase 1, was observed by the detection of in situ activities using this peptide Results Screening of candidate ... interaction of E51 with the enzyme to form an intermediate between the glutamine residue in the substrate and the cysteine residue in the active site pocket of TGase Assessment of reactivity and specificity...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
... for isoform 02C01 and comprises 17% of all fragment IIIvariants, while peptides IIIb and Xb are unique for 01B01 and comprise 1819% of all fragment III and X-variants The isoforms 02A01 and 02B01 ... presence and relative abundances of individual isoforms The pollen of four birch species contained a mixture of Bet v isoforms, with abundant levels of isoforms that were similar to isoforms with ... mixture of multiple isoforms and their posttranslational modifications, which could not be separate completely by 2-Dimension gel electrophoresis A disadvantage of using Q-TOF LC-MSE instead of Q-TOF...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
... CTLs and HTLV-I infected T cells We have previously established a rat model of ATL-like disease, which allows examination of the growth and spread of HTLV-I infected cells, as well assessment of ... Establishment of MOLT-4 cells stably expressing SCTs of RT1.Al To examine the effects of rat SCTs expressed on human cells and the influence of linker length on the activation of CTLs in more ... HTLV-I-infected rat and that the detection of the epitopespecific CTLs by SCT-EGFP system was comparable to the measurement of peptide-induced IFN-γ production Thus, the activation and detection system...
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Báo cáo sinh học: "Analysis of a simulated microarray dataset: Comparison of methods for data normalisation and detection of differential expression (Open Access publication)" pps
... (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS) and the number of methods in ... id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and SD LOESS), the number of methods in which ... simulation software Array number of grid rows Array number of grid columns Number of spots in a grid row Number of spots in a grid column Number of spot pins Number of technical replicates Number of genes...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... soyasapogenols A and B without producing artifacts (Ireland and Dziedzic 1986; Rupasinghe, Jackson et al 2003) Moreover, Ireland and Dziedzic (Ireland and Dziedzic 1986) showed that methylation for ... 79 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods...
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Analysis and detection of human motion in time frequency domain
... decomposition and reconstruction process, we still need to understand the nature of the frequency bandwidth of decomposed and reconstructed signals Table 3.1 shows the frequency bandwidths of decomposed and ... coefficients and 93 separated segments of human motion patterns in 12 the acceleration signals 4.20 44 pairs of Pa and Pv for ascending and descending stairs 96 and 66 pairs of Pa and Pv for level ... mean and one side standard deviation of positive peak 122-124 and negative peak angular rates of gyroscope sensors at position FW, S and RU Both positive and negative peak angular rates of fall...
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Analysis and detection of human emotion and stress from speech signals
... fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m th filter bank St ( m ) ... Kyi Tar Nwe and other family members for their support, understanding and encouragement i Table of Contents Acknowledgements i Table of Contents ii Summary vi List of Symbols viii List of Figures ... Motivation of This Research 1.5 System Overview 1.6 Purpose and Contribution of This Thesis 10 1.7 Organization of Thesis 11 Chapter 2: Review of Acoustic Characteristics and Classification Systems of...
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Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate
... one of the most powerful method based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand and then amplify thousands to millions of ... DNA Ladder was added and mixed 20 µl of products of PCR and µl of 6x Loading Dye were put into gel which contained 0.5 x TPE buffer to prepare 1.0% agarose gel and µL of EtBr And the electrophoresis ... fingerprints; and the detection and diagnosis of hazardous diseases etc (Bartlett and Stirling, 2003) Particularly, PCR is commonly used in selective DNA isolation and detection of bacteria or viruses...
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A065 seismic design, response modification and retrofit of bridge
... “Seismic Design, Response Modification and Retrofit of Bridges” at the Graduate Course of the Department of Civil Engineering, Tokyo Institute of Technology, Japan The scientific and engineering knowledge ... Composite-Materials Jackets 5) Retrofit of Steel Columns 8.4 Seismic Retrofit of Foundations 1) Seismic Retrofit of Foundations with Inadequate Soil Bearing Caqpacity 2) Seismic Retrofit of Pile Foundations ... Evaluation of Response Modification Factors b) Evaluation of Strength and Ductility Capacity c) Residual Displacement d) Design Detailings e) Comparison of Pre-Kobe and Post-Kobe Codes 6) Design of Foundations...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... Kasai, H., Sofuni, T and Nohmi, T (1997) Construction of mutants of Salmonella typhimurium deficient in 8-hydroxyguanine DNA glycosylase and their sensitivities to oxidative mutagens and nitro ... 233-246 Yamada, M., Sedgwick, B., Sofuni, T and Nohni T (1995) Construction and characterization of mutants of Salmonella typhimurium deficient in DNA repair of O6-methylguanine, J Bacteriology, ... 2-Nitrofluoranthene, 1-nitropyrene and 1,8-dinitropyrene are tested without S9mix, Glu-P-1 and Trp-P-2 are tested with S9mix using TA98 and YG1041 2-Nitronaphthalene, 2,4-dinitrotoluen and 4-nitroquinoline...
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Detection of bacteria and enteric viruses from river and estuarine sediment
... (1996) and Watson and Blackwell (2000) reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane filtration was yellow, both of ... procedure of Craig et al (2002) with some modifications: mL double-distilled water was used instead of mL 0.1% peptone water; a vortex mixer was used for 15 sec instead of hand - 310 - Journal of Water ... composition of silt and clay The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson...
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Detection of Antiviral Drugs Oseltamivir Phosphate and Oseltamivir Carboxylate in Neya River, Osaka, Japan
... Quantifications of OP, OC and OC-D3 were based on calibration curves from to 1000 ng/L The limit of detection (LOD) and limit of quantification (LOQ) values for OP, OC and OC-D3 in this investigation ... development of oseltamivir-resistant influenza viruses Furthermore, Japan is at the top of the list of countries for per capita consumption of Oseltamivir (Soderstrom et al., 2009), and it has ... influenza and H1N1 influenza pandemic (September 2009 to February 2010) MATERIALS AND METHODS Chemicals and Reagents Oseltamivir phosphate (CAS-RN: 204255-11-8) was obtained from F Hoffmann-La...
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Introduction to the basic approaches and issues of Intrusion Detection
... frequency of intrusion detection reporting * *Place “call pre-emption” capability (software and personnel) in standby mode Place “call pre-emption” capability (software and personnel) in standby ... significant change in system profile Attack Metrics • Severity is defined by the criticality of the target and lethality of the attack, and the effectiveness of system and network countermeasures ... discussion of basic approaches to intrusion detection We started this section with the discussion of Events of Interest (EOI) – a notion that personnel and computing resources are limited, and often...
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Tài liệu Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults pdf
... Features of ATP III similar to those of ATP I and II Identification of population groups, besides middle-aged men, for detection of high LDL cholesterol (and other lipid risk factors) and for ... Ref–1 xii Detection Evaluation Treatment I Background and Introduction I Background and Introduction The Third Report of the Expert Panel on Detection, Evaluation, and Treatment of High Blood ... as target of therapy Notwithstanding this diverse evidence, the ultimate proof of the benefits of lowering LDL cholesterol is through clinical trial A large number of clinical trials of cholesterol-lowering...
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Tài liệu Báo cáo khoa học: "Automatic Extraction of Lexico-Syntactic Patterns for Detection of Negation and Speculation Scopes" pdf
... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... also manually compiled and used in a number of hedge scope detection systems, e.g (Kilicoglu and Bergler, 2008), (Rei and Briscoe, 2010), (Velldal et al., 2010), (Kilicoglu and Bergler, 2010), (Zhou ... speculation and negation scopes could be concisely expressed as a 284 combination of lexical and syntactic patterns For ¨ u example, Ozg¨ r and Radev (2009) examined sample BioScope sentences and developed...
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Mammography and Beyond: Developing Technologies for the Early Detection of Breast Cancer pptx
... Institutes of Health National Institute of Standards and Technology National Mammography Database OBBB OTA OWH Office of Bioengineering, Bioimaging, and Bioinformatics Office of Technology Assessment Office ... research and development for early detection and diagnosis of breast cancer Earlier detection is one priority area of the program and encompasses the development of new technologies, biomarkers, and ... crucial to understand the value and limits of [screening] tests, and how they fit into the goals of medicine We are in danger of behaving as if all technological change is progress, and of confusing...
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Xpert MTB/RIF test for detection of pulmonary tuberculosis and rifampicin resistance (Protocol) pptx
... percentage of contaminated cultures; review question B: the name and manufacturer of the reference standard • Details of index test: software version of test • Details of comparator: type of microscopy: ... 2010a) Early and accurate diagnosis and effective treatment is the cornerstone of TB care and control (Dye 2010) A basic tenet of early and accurate diagnosis is the identification of smear-negative ... addresses question A (detection of MTB) and/ or B (detec- - A tion of RIF resistance)? 2-B - A and B What was the purpose of this study? * if smear results are stratified, - Only accuracy of Xpert as replacement...
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Perspectives of Chief Ethics and Compliance Officers on the Detection and Prevention of Corporate Misdeeds ppt
... of 2008 has aroused the interest of U.S policymakers in the mechanisms of corporate governance, compliance, and ethics, and their collective role in preventing and mitigating excesses and scandals ... options back-dating scandals and the mutual fund market-timing scandals of the mid-2000s Of course, the most recent set of corporate scandals has broadly swept across the mortgage and banking sectors, ... sector and other parts of the economy It is in this context that RAND convened a March 5, 2009, conference entitled “Perspectives of Chief Ethics and Compliance Officers on the Detection and Prevention...
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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx
... method in the case of particular products The harmonization of test methods cannot be immediate, and for certain groups of products International Standards and/ or national standards may already ... rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products [4] ISO/TR 11133-1, Microbiology of food and animal feeding stuffs ... International Standard is applicable to products intended for human consumption and the feeding of animals; environmental samples in the area of food production and food handling WARNING...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct các đặc tính của động cơ điện không đồng bộ đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25