biotechnological approaches to optimize cell wall performance in planta
plant biotechnology and transgenic plants - kirsi-marja oksman-caldentey , wolfgang h. barz
Ngày tải lên :
08/04/2014, 12:49
... thought to be cell wall stiffening and modification processes including cross-linking reactions of cell wall proteins (19) In connection with cell wall- located proteins, the large group of glycine-rich ... (scopolamine) (2) H Vinblastine and Vincristine Vinblastine and vincristine are binary indole alkaloids isolated from the aerial parts of the Madagascan periwinkle (Catharanthus roseus G Don syn Vinca ... derivatives of vinblastine are marketed, namely vindesine and vinorelbine Vindesine is prepared from vinblastine by formation of the hydrazide of 16-deacetylvinblastine (with hydrazine) and reduction...
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Báo cáo y học: " Low NO bioavailability in CCl4 cirrhotic rat livers might result from low NO synthesis combined with decreased superoxide dismutase activity allowing superoxide-mediated NO breakdown: A comparison of two portal hypertensive rat models wit
Ngày tải lên :
13/08/2014, 13:20
... stellate cells [34] and hepatocytes [21] were identified as important endothelin-1 synthesising cells An increased endothelin-1 synthesis together with changes in endothelin-A and B receptor density ... CCl4 induced cirrhosis was obtained by 12 weekly inhalations (Table 1) or ingestion (Table 2) of the hepatotoxin CCl4, together with phenobarbital 350 mg/l in the drinking water [39] Rats were studied ... homogenates containing 30 µg of protein and with marker proteins (Sigma, St Louis, USA) including a 120 kD protein, E coli β-galactosidase All protein concentrations were measured using the Bradford...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... once again on lm paraquat-containing plates This tertiary screening step helped eliminate false positives due to mutations in the bacterial chromosome or in the backbone of the expression vector ... Measured at pH 9.0 by stopped-flow spectrophotometry e Catalysis was too rapid and product inhibition too strong for these values to be determined by stopped-flow spectrophotometry f These mutants ... thought to play an important role in altering the function of Q143A hMnSOD to better protect QC774 E coli cells from superoxide toxicity tions result in a shorter side chain, decreasing the likelihood...
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Báo cáo Y học: ER–resident chaperone interactions with recombinant antibodies in transgenic plants potx
Ngày tải lên :
31/03/2014, 08:20
... identified in insect cell lines, candidate insect protein chaperones have been identified that may perform a similar role [47] Indeed, when insect cells were engineered to express murine BiP, increased ... immunoglobulin chains in plants To investigate the specific association of BiP with recombinant immunoglobulin chains, we prepared protoplasts Fig Western blot analysis of total BiP protein in plant ... proteins [9,49] and in folding and assembly of immunoglobulin chains in cells that are highly active in terms of recombinant protein production and secretion The relative decrease in BiP mRNA in...
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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx
Ngày tải lên :
20/06/2014, 01:20
... predominant variants found in vaginal secretions, and assessed ART use in a cohort of 43 women attending an HIV outpatient clinic in New Orleans Informed consent was obtained in accordance with Louisiana ... were lower in ART users as compared to nonusers, however ART users represented more than half of the women in this shedding cohort, demonstrating that vaginal HIV shedding occurs in a number ... CCR5: Chemokine (C-C motif) receptor 5; CXCR4: Chemokine (C-X-C motif) receptor 4; RNA: Ribonucleic acid; PCR: Polymerase chain reaction; IRB: Institutional review board Competing interests The...
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Báo cáo y học: "A simple hepatic cyst with elevated serum and cyst fluid CA19-9 levels: a case report" pps
Ngày tải lên :
11/08/2014, 21:22
... concomitant with increased flow of necrotic cyst wall cells after the minocycline instillation, suggesting that CA19-9 may originate from the hepatic cyst wall cells Inflammation, including infection ... an infected hepatic cyst 400 mg of minocycline hydrochloride was dissolved in 50 ml of saline, and this minocycline solution was injected into the cyst using the drainage catheter The drain was ... day after and week after intracystic instillation of minocycline hydrochloride After intracystic instillation, necrotic cells in the cyst were drained, and the cyst totally col- Page of (page...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
Ngày tải lên :
14/02/2014, 22:20
... cofactor, respectively SODs are grouped into four classes according to their metal cofactors: copper and zinc-containing SOD (Cu,Zn-SOD), iron-containing SOD (Fe-SOD), manganese-containing SOD ... incorporated into the enzyme as it accumulated in the E coli cells Indeed, the activity of the apo-enzyme was significantly lower than the metal-containing enzyme Metal cofactors were incorporated into ... reasonable to conclude that the dominant electrostatic interaction in ApeSOD contributed not only to heat tolerance, but also to tolerance of the enzyme to organic environment For enzymes to be used in...
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Tài liệu Design of Feedback Control Systems for Stable Plants with Saturating Actuators ppt
Ngày tải lên :
18/02/2014, 10:20
... system towards [.3 - 3]T resulting in oscillatory behavior The compensator does not have any integrators to cause windups and the problems in the performance of the system are solely due to the ... the compensator was designed to invert or partially invert the plant, then the inversion process will not be distorted by the saturation and GsatK will remain linear and equal to GK In the closed ... to the maximum gain X (to) is chosen such that < X (to) < and x(t)e BA,CVt > to If a greater gain X (to) is used then g(x (to) will be increasing (see theorem 3.2) and x(t)o BA,CVt >to; consequently...
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Tài liệu Báo cáo khoa học: The solution structure of reduced dimeric copper zinc superoxide dismutase doc
Ngày tải lên :
21/02/2014, 03:20
... peaks due to couplings between the two subunits were converted into upper distance limits using specific calibration curves In the case of protons belonging to the ligand histidines an independent ... guanidinium group of Arg143 [31], which is located in the active site channel pointing towards the copper ion and whose side-chain orientation is optimized for correctly orienting the incoming ... protein was taken into account by connecting the amino acid sequence of the two subunits with a chain of linkers composed of atoms with a null Van der Waals radius The metal ions were included in...
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Báo cáo khoa học: A superoxide dismutase–human hemoglobin fusion protein showing enhanced antioxidative properties pptx
Ngày tải lên :
07/03/2014, 00:20
... dissociate into dimers and monomers In addition to dissociation into dimers, the complexity of the DSC curve is increased by the thermal inactivation of the SOD enzymatic activity, resulting in smaller ... tryptophan synthase Besides generating homogeneous conjugates, this method also allows for in vivo testing of the fusion proteins, as opposed to chemical approaches Superoxide is intrinsically toxic, ... generated during the autoxidation of 1-methyl-4-phenyl-2,3-dihydropyridinium, a bioactivated intermediate of parkinsonian-inducing neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine J Biol...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
Ngày tải lên :
16/03/2014, 01:20
... selective cytotoxicity in HBV-infected hepatocytes We first examined the cytotoxicity of BetA in wildtype (WT) and HBV-infected hepatocytes Different dosages of BetA were used to treat the cells for ... synthesis rate of HBV-infected cells was substantially inhibited, whereas no difference was found in WT cells, indicating that BetA-induced cytotoxicity was specific to HBV-infected cells FEBS Journal ... CREB, in turn, promotes recruitment of the coactivator paralogs CREB-binding protein and p300 via a kinase-inducible domain in CREB, which appears to be sufficient for the induction of cellular...
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