biotechnological approaches to optimize cell wall components for uses ex planta
plant biotechnology and transgenic plants - kirsi-marja oksman-caldentey , wolfgang h. barz
Ngày tải lên :
08/04/2014, 12:49
... of cells can be turned into a state of practically uniform cells, this much less complicated cellular system can then be exploited to study many problems This has been performed with plant cell ... depends to some extent on the tissue and cell culture techniques (4) Plants are characterized by totipotency, which means that each cell possesses and can express the total genetic potential to form ... transformation vectors in order to target transgenes by homologous recombination The transplastomic tomato plants finally obtained were shown to transfer the foreign gene to the next generation via uniparentally...
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Báo cáo y học: " Low NO bioavailability in CCl4 cirrhotic rat livers might result from low NO synthesis combined with decreased superoxide dismutase activity allowing superoxide-mediated NO breakdown: A comparison of two portal hypertensive rat models wit
Ngày tải lên :
13/08/2014, 13:20
... vasodilator acting through activation of soluble guanylyl cyclase in vasoactive effector cells Superoxide is able to react with NO to form reactive nitrogen species, which could not have vasodilatory ... endothelial cells (Transduction Labs, Lexington, USA) for eNOS; a homogenate of rat brains for nNOS; and a liver of a rat given LPS 800 µg/kg IV (Sigma, St Louis, USA) hours before harvesting for iNOS ... different isoforms in all liver cell types [15] Admittedly, we did not study SOD activity in particular liver cell types or in the vascular lumen (the latter regards the extracellular SOD isoform) SOD...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
Ngày tải lên :
07/03/2014, 11:20
... stopped-flow spectrophotometry e Catalysis was too rapid and product inhibition too strong for these values to be determined by stopped-flow spectrophotometry f These mutants had a very small extent ... QC774 cells, and this transformant was plated onto a lm paraquat plate so as to obtain approximately 4000 transformants The growth (or lack of growth) of these Q143A hMnSOD-expressing cells on ... paraquat for each randomly point-mutagenized library In addition, a control transformation was performed together with each library, whereby the plasmid expressing Q143A hMnSOD was used to transform...
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Báo cáo Y học: ER–resident chaperone interactions with recombinant antibodies in transgenic plants potx
Ngày tải lên :
31/03/2014, 08:20
... heterologous proteins has not been performed The aims of this study were to determine how the plant ER responds to the expression of heterologous secretory proteins We therefore studied the involvement ... Western blotting and ELISA To confirm the expression of each immunoglobulin chain, leaf extracts were examined by Western blot analysis, using specific antisera [5] For total BiP assay, mm leaf punches ... was allowed to proceed for 10 min, then 25 lL of M H2SO4 were added to each well and the absorbance read at 450 nm on an ELISA plate reader (Anthos, UK) RNA extraction Total RNA was extracted from...
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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx
Ngày tải lên :
20/06/2014, 01:20
... Monitor Assay, according to assay protocol Samples from a cohort of 43 women with measurable levels (>50 copies/sample) of vaginal HIV-1 RNA were available for genotypic analysis in this study To ... Health Excellence Fund, the Centers for Disease Control and Prevention RA1/CCR622272, and the Gulf South STI/Topical Microbicide Cooperative Research Center NIAID 1U19 AI61972-01 References Due to ... vaginal sample This protocol has been utilized and validated for analyses of HIV genotypes and prediction of coreceptor usage [9-13] The genotypic evaluations by heteroduplex analysis in this study...
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Báo cáo y học: "A simple hepatic cyst with elevated serum and cyst fluid CA19-9 levels: a case report" pps
Ngày tải lên :
11/08/2014, 21:22
... 93:2551-2555 Sawabu N, Takemori Y, Toya D, Yoneshima M, Kidani H, Satomura Y, Ohta H, Hattori N: Factors affecting serum levels of CA 19-9 with special reference to benign hepatobiliary and pancreatic ... thickened cystic wall B Figure A) Enhanced abdominal computed tomography before a percutaneous transhepatic drainage of the hepatic cyst A) Enhanced abdominal computed tomography before a percutaneous ... Written informed consent was obtained from the patient for the publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
Ngày tải lên :
14/02/2014, 22:20
... reasonable to conclude that the dominant electrostatic interaction in ApeSOD contributed not only to heat tolerance, but also to tolerance of the enzyme to organic environment For enzymes to be used ... length (A) rmsd bond angle (°) Protein atoms Metal atoms Ethylene glycol molecules Water molecules Average B-factor Protein atoms Solvent atoms Metal atoms Ramachandran plot (%)e Favored Allowed ... University) for performing ultracentrifugation analysis and Mr K Mieda and M Sakata (Tottori University) for technical assistance in enzyme assay This study was supported by a Grant-in-Aid for Scientific...
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Tài liệu Design of Feedback Control Systems for Stable Plants with Saturating Actuators ppt
Ngày tải lên :
18/02/2014, 10:20
... every time to, if x (to) e BA,C then the time-varying gain X (to) is the maximum possible such gain that < X (to) < and Vu(t), t >to X(t), t > to such that the output Iyi(t)l < V i, t >to If x (to) v BA,C ... X (to) does not exist Proof: If x (to) E BA, C , then from the construction of X(t), at any time to the maximum gain X (to) is chosen such that < X (to) < and x(t)e BA,CVt > to If a greater gain X (to) ... without the EG operator, integrator windups would be expected and the direction of the control vector would be distorted Three simulations were performed to show the integrator windup problem...
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Tài liệu Báo cáo khoa học: The solution structure of reduced dimeric copper zinc superoxide dismutase doc
Ngày tải lên :
21/02/2014, 03:20
... v1 torsion angles from the HNHB spectrum A total of 45 proton pairs were stereospecifically assigned with the program GLOMSA: 16 protons belonging to bCH2, 11 to aCH, to cCH2, to dCH3 and to cCH3; ... 800 MHz, experiments, were performed to determine the 3JHNHa coupling constants and additional constraints for the v1 torsion angles as well as stereospecific assignments for the Hb protons The ... the HNHB experiment In each subunit the metal ions were included by allowing copper to bind to Ne2 of His48 and His120 and to Nd1of His46, and zinc to Nd1 of His63, His71, and His80 and to Od1...
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Báo cáo khoa học: A superoxide dismutase–human hemoglobin fusion protein showing enhanced antioxidative properties pptx
Ngày tải lên :
07/03/2014, 00:20
... The expression level was lower for SOD–Hb than for wild-type Hb, but may be further optimized by introducing compensatory mutations to increase expression and improve heme transport into the cell ... showing possible conformations resulting in (from top to bottom): the high molecular mass complex, an SOD–Hb dimer, and finally an SOD–Hb monomer A Autoxidation studies were performed at room temperature ... metHb formation [3] and eventually lead to heme degradation The catalase present intracellularly may be sufficient to remove H2O2 by conversion to water [4]; however, there is not sufficient SOD to...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
Ngày tải lên :
16/03/2014, 01:20
... selective cytotoxicity in HBV-infected hepatocytes We first examined the cytotoxicity of BetA in wildtype (WT) and HBV-infected hepatocytes Different dosages of BetA were used to treat the cells for 48 ... selective cytotoxicity in HBV-infected hepatocytes We have found that BetA has little cytotoxic effect on WT hepatocytes, but shows a selective cytotoxic effect on HBV-infected hepatocytes In ... WT cells, whereas BetA treatment caused significant cytotoxicity in HBV-infected cells (Fig 1A) Also, the time course results showed that WT cells were more resistant to BetA-mediated cytotoxicity...
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