bioremediation of seleniferous water and sediment
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 21 (end) pps
... involved, and its nature and mode of action; specificity for substrate; nature of the chemical structure; and specificity to inhibitors Active center: Knowledge of the nature and composition of the ... phospholipids that are water soluble can be used as synthetic substrates and choice of substrate can often distinguish the different types of lipases and phospholipases Table Summary of Some of the Major ... variety of reagents (68) Advancement of a scientific discipline is often closely tied to the development of new and improved methods and equipment The explosion of electronic devices and largescale...
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báo cáo hóa học: " Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity" pptx
... 1630, and 433 cm-1 corresponding to water (moisture) and ZnO nanocrystal, the peaks at 2936.3 and 2872 cm -1 of the C-H stretching Page of vibration [26], and the peaks at 1330 and 1560 cm-1 of ... amounts of amino groups on Figure SEM images of ZnO nanocryatals (a) Nanospheres, (b) nanodisks, and (c) nanomultipods prepared using mixtures of methanol and water with different volume ratios of ... mixtures of methanol /water with different volume ratios The surfaces of Page of ZnO nanocrystals were modified with amino groups using TEOS and APTES to study the effect of the morphology of the...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... 3A) Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... coordinated with six ligands: the five same ligands in the Mn-reconstituted enzyme and an additional water oxygen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed ... polypeptides consisted of seven a-helices, a three-stranded antiparallel b-sheet and loops connecting these secondary structure elements The contents of the a-helix and b-strand were 50% and 11%, respectively...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... understanding of the generic principle of catalysis for this family of proteases Results and Discussion Fold of ervatamin-A The overall structure and fold of ervatamin-A is similar to that of ervatamin-B, ... rounds of positional refinement, fitting, and introduction of water molecules and SO42), followed by manual fitting of the model by quanta (Accelrys Inc.), led to an R-factor of 19.78% and Rfree of ... [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active site cleft at the interface of the domains (Fig 1) Substrate specificity and activity of...
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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx
... P5, P6 and minor parts of P5a and P6a are protected Also, J4 is weakly protected, but J5 is weakly cleaved Of particular signicance is the observed cleavage of J6 and the neutrality of J3 ... three nucleotides of P7, and the 3Â-half of J8 7, are not protected The rst three nucleotides of P7 are part of the G binding site, as are the terminal nucleotides of J6 and J8 [3537]; the ... cycles of 94 C, 65 C, and 72 C (1 each) Transcription of PCR23S.5DGb and purication of the RNA on denaturing gels was performed as described [18] G-dependent cleavage of 23S.5DGb pre-RNA, and quantication...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
... single and dual binding site mutants [29] The homologous SBD of Aspergillus niger glucoamylase had Kd values of 6.4 and 28 lm for b-CD and of 0.95 and 17 lm for maltoheptaose for each of the ... from a test set of 5% of the reections randomly selected from all data Based on inspection of 2Fo-Fc and Fo-Fc maps (contoured at and r, respectively), calcium ions were inserted and water molecules ... (2001) Modulation of activity and substrate binding modes by mutation of single and double subsites +1 +2 and )6 of barley a-amylase Eur J Biochem 268, 65456558 11 Bak-Jensen KS, Andre G, Gottschalk...
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Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc
... Stereoview of the alignment of the active sites of AfRio1 (green) and AfRio2 (orange; PDB code 1ZAO) (D) Stereoview of the alignment of the active sites and bound nuceotide of AfRio1 (green) and PKA ... (H1) and myelin basic protein (MBP) (H) Autophosphorylation activity of equivalent amounts of AfRio1 and AfRio2 in the presence of ATP and GTP H D E conservation of residues at the end of strand ... structure of Rio2 bound to ATP and Mn2+, the c-phosphate is buried through the ordering and binding of three residues of the N-terminal end of the flexible loop [21] The P-loops of Rio1 and Rio2...
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báo cáo khoa học: "Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination" pot
... work will contribute to our understanding of the function and evolution of the maize SBEs, and of their roles in the biosynthesis, hydrolysis and utilization of starch granules Moreover, the novel ... produced Analysis of the CL distribution of isoamylase-debranched b-LDs showed a higher proportion of chains of DP ≥ 100 and lower proportions of other chains (DP < 100), before and after pullulanase ... proportion and a reduced branching density of endosperm amylopectin [5,33-35] More recently, studies of a maize sbe2a mutant showed that deficiency of the SBEIIa isoform decreased plant fitness and...
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Báo cáo khoa học: " Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains" potx
... of rNTPs by wild-type RTs and their chimeric derivatives Use of rNTPs by wild-type RTs and their chimeric derivatives The ability of reverse transcriptases from the wild type HIV-1 and MuLV and ... cleavage pattern of the 5'-32P-RNA strand of the duplex by wild-type enzymes and their chimeric derivatives As shown in panel A of Figure 5, the initial cleavage of the 30-mer RNA strand by the wild-type ... construction and cloning of chimeric RTs, characterization of their polymerase and RNase H activities and wrote the manuscript AU performed DNA binding studies and determination of metal specificity...
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Computational methods for structure activity relationship analysis and activity prediction
... Molecules of Different Origins have Distinct Distributions of Structural Complexity that Correlate with Protein-Binding Profiles Proceedings of the National Academy of Sciences of the United States of ... doctoral studies and for his constant guidance and support I thank Prof Dr Michael G¨ utschow for reviewing my thesis as a co-referent I also thank Prof Dr Thorsten Lang and Prof Dr Thomas Schultz ... The assessment of structural similarity of compounds depends on the computational representation of molecules and the similarity metric Hence, the choice of the representation and similarity...
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Impact of pH on Anaerobic Substrate Uptake by PAOs and GAOs in an EBPR Activated Sludge Process Analyzed by MAR-FISH
... Journal of Water and Environment Technology, Vol 7, No 4, 2009 Since the predomination of GAOs instead of PAOs leads to the deterioration of EBPR, it is essential to understand the mechanisms of the ... exposure times were hrs (Candidatus ‘Competibacter phosphatis’ for the first and second experiments and Candidatus ‘Accumulibacter phosphatis’ for the - 217 - Journal of Water and Environment Technology, ... between and or The effect of pH on the anaerobic uptake of radio-labeled acetate by Candidatus ‘Accumulibacter phosphatis’ and Candidatus ‘Competibacter phosphatis’ was examined by MAR-FISH, and...
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Tài liệu Gravitational Physics: Exploring the Structure of Space and Time pdf
... development of string theory and the quantum theory of geometry as promising candidates for the union of quantum mechanics and general relativity • The first descriptions of the quantum states of black ... of that matter there is, and the curvature of space on large scales • The unambiguous measurement of the value of the cosmological constant, with profound implications for our understanding of ... constant, with profound implications for our understanding of the fate of the universe, and also for particle physics and quantum gravity • The use of gamma-ray, x-ray, optical, infrared, and radio...
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Tài liệu FLUID-STRUCTURE INTERACTIONSSLENDER STRUCTURES AND AXIAL FLOW VOLUME 1 ppt
... Thompson of University College London, S.S Chen of Argonne, E.H Dowel1 of Duke, C.D Mote Jr of Berkeley, F.C Moon of Cornell, J.P Cusumano of Penn State, A.K Bajaj of Purdue, N.S Namachchivaya of the ... Namachchivaya of the University of Illinois, S Hayama and S Kaneko of the University of Tokyo, Y Sugiyama of Osaka Prefecture, M Yoshizawa of Keio, the late Y.Nakamura of Kyushu and many others, too numerous ... on the existence and stability of limit-cycle motions, and the possible existence of chaotic oscillations This necessitates the introduction and utilization of some of the tools of modem dynamics...
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Tài liệu Child mental health and educational attainment: multiple observers and the measurement error problem ppt
... assessment of themselves rather than those of parents and teachers A clear implication of this limited body of evidence is that measurement error is substantial and unlikely to be the simple random ... of the process of child development are often the focus of special attention, while the problems of older children and adolescents are often less visible to external observers and seem also to ... independently of cognitive ability, a stock of ‘noncognitive skills’ are built up by streams of investment over the life course and determine a wide range of life outcomes (Heckman, Stixrud and Urzua,...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... concentrate on applications of COFRADIC in studying selected post-translational modifications ) protein processing [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions ... features of ATP-binding proteins As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules However, several proteins and ... autosampler, and can be purchased from a variety of companies; and, at least in our hands, HPLC solvent gradients and flow rates can nowadays be controlled such that the overall reproducibility of HPLC...
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Social Audit: A Toolkit A Guide for Performance Improvement and Outcome Measurement doc
... carefully Survey of attitudes and behaviour of stakeholders concerned: A survey of attitudes and behaviour of an adequate number of stakeholder groups will be useful in gathering significant and reliable ... Distribution of power and authority Identification of stakeholders Interested and affected public Leadership capability and characteristics Individual and Family Changes Perceptions of risk, health and ... participation of community, state everyone, equity in distribution of social benefits, (constitution and inclusiveness (women and disadvantaged), well being of people and sustainability of all these...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... concentration [N] and the results of the inhibition studies show that, despite of the high rate of hydrolysis of the acyl-enzyme, tight binding of the nucleophile and displacement of the catalytic H2O molecule ... 7) The Vs/Vh ratio is the ratio of the reaction rates of aminolysis and hydrolysis of the acyl-enzyme The curvature of the plot of Vs/Vh vs the mole fraction of D2O indicates therefore that the ... [AD] ð2Þ Table Steady-state parameters of penicillin acylase for the hydrolysis of esters and amides of phenylacetic acid and phenylglycine The structures of the chromogenic substrates are shown...
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Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx
... 5-isothiocyanate and PI Fluorescence emission of fluorescin 5-isothiocyanate and PI from single cells were separated and measured using the standard optics of the FACSort The CELLQUEST software program ... batch of cells, the SCA was calculated by: SCA ¼ ðAr À AvÞ=ðFr À FvÞ where Ar and Av are the cAMP (or IP) of cells transfected with the mutant constructs and vector, respectively, and Fr and Fv ... of improving our understanding of the mechanisms underlying some of these characteristics, we have explored the effects of combining activating and inactivating mutations of the TSHr within a single...
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Gravitational Physics Exploring the Structure of Space and Time docx
... development of string theory and the quantum theory of geometry as promising candidates for the union of quantum mechanics and general relativity • The first descriptions of the quantum states of black ... of that matter there is, and the curvature of space on large scales • The unambiguous measurement of the value of the cosmological constant, with profound implications for our understanding of ... constant, with profound implications for our understanding of the fate of the universe, and also for particle physics and quantum gravity • The use of gamma-ray, x-ray, optical, infrared, and radio...
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