bioremediation of chromium contaminated water
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 21 (end) pps
... network of organomineral complexes (13,65) III ROLE OF CHEMISTRY IN ENZYME ACTIVITY MEASUREMENT One of the fundamental requirements of enzyme measurements is a thorough understanding of the reactions ... ϩ log A), allows calculation of Ea by plotting log of the initial rate of the reaction vs 1/T The slope of the resulting line is equal to ϪEa/2.303R The enthalpy of activation (∆Ha) can be calculated ... not soluble in water and this makes the design of an assay difficult Shortchain phospholipids that are water soluble can be used as synthetic substrates and choice of substrate can often distinguish...
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báo cáo hóa học: " Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity" pptx
... mixtures of methanol /water with different volume ratios The surfaces of Page of ZnO nanocrystals were modified with amino groups using TEOS and APTES to study the effect of the morphology of the ... corresponding to water (moisture) and ZnO nanocrystal, the peaks at 2936.3 and 2872 cm -1 of the C-H stretching Page of vibration [26], and the peaks at 1330 and 1560 cm-1 of the stretching vibration of C-N ... presence of amino groups on the ZnO nanocrystals surface The amount of amino groups and the thickness of the coating layer can be controlled by adjusting the ratio of TEOS to APTES When the ratio of...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... 3A) Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor Figure 3D shows the superimposition of the active site structures of apo, Mn-bound and Fe-bound ApeSODs ... an additional water oxygen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed an equatorial plane (Fig 3B) The metal ion and the addi˚ tional water oxygen...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... rounds of positional refinement, fitting, and introduction of water molecules and SO42), followed by manual fitting of the model by quanta (Accelrys Inc.), led to an R-factor of 19.78% and Rfree of ... activity of the enzyme, which has the catalytic site at the interface of the two domains This observation helps us to improve our understanding of the generic principle of catalysis for this family of ... Discussion Fold of ervatamin-A The overall structure and fold of ervatamin-A is similar to that of ervatamin-B, ervatamin-C [27,35] and other members of the papain family, and is made up of two domains,...
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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx
... leads to formation of the active complex, whereas binding of a minimum of m Mn2+ ions forms the inactive complex The sizes of the active and inactive fractions are the result of competitive metal ... presence of Mn2+ The midpoint of the Mg2+ titration curve in the absence of Mn2+ is approximately 4.5 mm, and nearly full activity is reached by 10 mm Mg2+ Hill analysis of the data gives n-values of ... parts of P7, J8 and P9.0 protected The Cr.LSU pattern is distinct in that the rst three nucleotides of P7, and the 3Â-half of J8 7, are not protected The rst three nucleotides of P7 are part of...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
... reported hydrolysis of the amylose of DP440 in a multiple attack mechanism [12] was conrmed for AMY1, which showed a DMA of 1.9 as determined from the ratio of rates of release of reducing groups ... the presence of b-CD (Fig 4; Table 4) due to competition with starch granule binding The low activity hampered analysis of the effect of b-CD on AMY1 Y380A Fig Rates of release of soluble reducing ... against granular starch, with release of soluble reducing sugars from this substrate being strongly reduced (Fig 4) to a kcat Km value of $10% of that of AMY1 wild-type In contrast to wild-type,...
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Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc
... (C) Stereoview of the alignment of the active sites of AfRio1 (green) and AfRio2 (orange; PDB code 1ZAO) (D) Stereoview of the alignment of the active sites and bound nuceotide of AfRio1 (green) ... Photon Source, Argonne National Laboratory Use of the Advanced Photon Source was supported by the U S Department of Energy, Of ce of Science, Of ce of Basic Energy Sciences, under Contract No W-31-109-Eng38 ... determination of the structure of Rio1 and the availability of the previously determined structure of Rio2, has enabled us to define the minimal consensus RIO domain (Fig 1A) Similar to ePKs, it consists of...
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báo cáo khoa học: "Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination" pot
... level of RS is a measure of the extent of digestion by this enzyme An official in vitro method (AOAC 2002.02) is used for determination of the RS level This method was modified to allow study of ... genotypes, b-LD was produced Analysis of the CL distribution of isoamylase-debranched b-LDs showed a higher proportion of chains of DP ≥ 100 and lower proportions of other chains (DP < 100), before ... proportion and a reduced branching density of endosperm amylopectin [5,33-35] More recently, studies of a maize sbe2a mutant showed that deficiency of the SBEIIa isoform decreased plant fitness and resulted...
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Báo cáo khoa học: " Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains" potx
... of HIV-1 RT [11,12] Considerable homology exists between the RNase H domains of retroviral RTs and the E coli RNase H [13-16] In a model of the MuLV RT RNase H domain based on the structure of ... polymerase domain of HIV-1 RT on the RNase H domain Neither the degree of functional interdependence of these domains for their enzymatic activities nor the precise nature of their effect on ... bovine serum albumin; and 100 ng of enzyme in a final volume of μL Reactions were done at 37°C for variable times and terminated by the addition of equal volumes of Sanger's gel loading dye [43]...
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Computational methods for structure activity relationship analysis and activity prediction
... Molecules of Different Origins have Distinct Distributions of Structural Complexity that Correlate with Protein-Binding Profiles Proceedings of the National Academy of Sciences of the United States of ... The choice of molecular representation and the method of assessing similarities affects the outcome of the SAR analysis Thus, alternative methods providing distinct points of view of SARs are ... consistent manner The assessment of structural similarity of compounds depends on the computational representation of molecules and the similarity metric Hence, the choice of the representation and...
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Impact of pH on Anaerobic Substrate Uptake by PAOs and GAOs in an EBPR Activated Sludge Process Analyzed by MAR-FISH
... Journal of Water and Environment Technology, Vol 7, No 4, 2009 Since the predomination of GAOs instead of PAOs leads to the deterioration of EBPR, it is essential to understand the mechanisms of the ... amount of carbon uptake could not be compared On the other hand, within the same bacteria, MAR was proved to be an effective tool to compare the amount of carbon uptake - 218 - Journal of Water ... and the area of silver granules per cell Dotted lines are for 95% limits for the mean (Left: E coli Right: R eutropha ) Effect of pH on acetate uptake by PAOs and GAOs The profiles of pH, acetate...
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Tài liệu Gravitational Physics: Exploring the Structure of Space and Time pdf
... president of the National Academy of Sciences The National Academy of Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding ... the expansion of opportunities in both experiment and theory has made it one of the most rapidly changing areas of science today A short list of some of the important achievements of the past decade ... of that matter there is, and the curvature of space on large scales • The unambiguous measurement of the value of the cosmological constant, with profound implications for our understanding of...
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Tài liệu FLUID-STRUCTURE INTERACTIONSSLENDER STRUCTURES AND AXIAL FLOW VOLUME 1 ppt
... Dowling of Cambridge, J.M.T Thompson of University College London, S.S Chen of Argonne, E.H Dowel1 of Duke, C.D Mote Jr of Berkeley, F.C Moon of Cornell, J.P Cusumano of Penn State, A.K Bajaj of Purdue, ... N.S Namachchivaya of the University of Illinois, S Hayama and S Kaneko of the University of Tokyo, Y Sugiyama of Osaka Prefecture, M Yoshizawa of Keio, the late Y.Nakamura of Kyushu and many ... development of theory for certain classes of dynamical systems, and of new analytical methods for such systems, (ii) the understanding of the dynamics of more complex systems (covered in Chapters 6-11 of...
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Tài liệu Child mental health and educational attainment: multiple observers and the measurement error problem ppt
... since the early stages of the process of child development are often the focus of special attention, while the problems of older children and adolescents are often less visible to external observers ... that one of the three types of observer is unbiased and we now explore the implications 21 of this for the mental health-education parameter ρ The lower panel of Table reports the estimate of the ... estimate of the impact of mental disorder on educational progress (which exploits the specific structure of our measure of educational achievement) is considerably smaller than the range of estimates...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... proteomics techniques [72], COFRADIC has a number of unique properties As COFRADIC is essentially a peptide-sorting technology by which only a set of peptides representative of the proteomic problem...
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Social Audit: A Toolkit A Guide for Performance Improvement and Outcome Measurement doc
... Welfare Officer GoAP - Government of Andhra Pradesh GoI - Government of India HWO - Hostel Welfare Officer MIS - Management Information System MLA - Member of Legislative Assembly MP - Member of Parliament ... matters, like behaviour of employees, incidence of tax, image of local officials etc Charles Medawar pioneered the concept of Social Audit in 1972 with the application of the idea in medicine ... rating of the public agencies in terms of public satisfaction with different dimensions of their services Public feedback was also used to quantify the extent of corruption and other The goal of...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... reactive ester of NIPGB in kinetic experiments, failed because of the high rates of spontaneous hydrolysis of this compound The only substrate of PA for which the steady-state rate of hydrolysis ... analysis of penicillin acylase (Eur J Biochem 270) 3681 Fig Inhibition of NIPGB hydrolysis by 7-ADCA Lineweaver–Burk plot of the effect of various concentrations of 7-ADCA on the conversion of NIPGB ... concentration [N] and the results of the inhibition studies show that, despite of the high rate of hydrolysis of the acyl-enzyme, tight binding of the nucleophile and displacement of the catalytic H2O molecule...
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Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx
... which binding of the hormone to the N-terminal domain of the receptor translates into activation of its serpentine domain is unknown With the aim of improving our understanding of the mechanisms ... temperature for h in the presence of about 90 000 c.p.m [125I]bTSH (a gift of BRAHMS, Berlin, Germany) and the appropriate concentrations of cold bTSH At the end of the incubation cells were rinsed ... form of the receptor; the active conformation capable of promoting GDP/GTP exchange on the G-protein is achieved by a fraction of the unliganded receptors only, but is stabilized by binding of...
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Gravitational Physics Exploring the Structure of Space and Time docx
... president of the National Academy of Sciences The National Academy of Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding ... the expansion of opportunities in both experiment and theory has made it one of the most rapidly changing areas of science today A short list of some of the important achievements of the past decade ... of that matter there is, and the curvature of space on large scales • The unambiguous measurement of the value of the cosmological constant, with profound implications for our understanding of...
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