biomedical applications of chitin and chitosan derivatives

... cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and ... #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of ... membranes of the central vacuole, and the wall thickening of xylem cells (fi) common with other plant peroxidases [18,21,22] The inclusion of Ser96 and Asp99 in a salt bridge motif at the beginning of...

Ngày tải lên: 23/03/2014, 09:21

... speculated that the physical role of MeJA is to mobilize JA [19] The proof of this hypothesis might come from a more detailed understanding of how plants form MeJA from JA, and vice versa A recent study ... cycles of 30 s at 94 °C, at 40 °C and at 72 °C, followed by 30 cycles of 30 s at 94 °C, at 45 °C and at 72 °C Gel electrophoresis, DNA elution and modification was carried out according to standard ... biological activity of the two compounds and usually only JA content is measured If values of both JA and MeJA are published, the ratios depend on the plant species and the tissue analysed and vary from...

Ngày tải lên: 16/03/2014, 18:20

... to the understanding of the underlying mechanism of calcium metabolism in the process of shell and pearl formation, but also offer the opportunity to promote the yield and quality of pearl In this ... second and third EF-hand domains in oyster CaM and CaLP, while it reflected Ca2+-binding to the first and second EF-hand domains in the case of VanScyoc et al CaLP and CaM chromatography of extracts ... major component of oyster shell as well as a key intracellular second messenger The shells of oyster consist of 90% CaCO3, products of calcium metabolism, and a few percent of matrix of biological...

Ngày tải lên: 16/03/2014, 23:20

... isolated two positive clones of 1324 and 1319 bp, designated DpAR1 and DpAR2, respectively The nucleotide sequences of DpAR1 and DpAR2 have been submitted to the EMBL database and are available under ... sequences were determined for both strands of the cDNAs and analysed by the DNASTAR program package (Lasergene) and CLUSTALW Plant treatments To determine the effects of several stress conditions on ... protocol of Gartner et al [9] and the ¨ subsequent amino acid sequencing The second strategy is based on the use of orthologous genes for screening a cDNA library of D purpurea As such a type of steroid...

Ngày tải lên: 18/03/2014, 01:20

... kit TnT (Promega, Leiden, the Netherlands) under the control of T7 promoter using pcDNA3.HIS, and the quality of synthesis was verified by SDS/PAGE and exposure of the dried gel GSt or GStp76RBE ... representation of (A) the p76RBE protein and (B) the human genomic structure of p76RBE gene (A) Schematic representation of the 686 amino acid p76RBE protein with delimitation of both HR-1 and PDZ domains ... domains Regions of homology with proteins of other species are underlined and the percentage of amino acid identity is indicated (B) Schematic representation of the human genomic structure of p76RBE...

Ngày tải lên: 23/03/2014, 21:20

... life, and for being my unfailing source of strength and help and hope throughout these years and this thesis i Table of Contents TABLE OF CONTENTS Acknowledgements i Table of Contents ii List of ... µl of 70% ethanol and air-dried The DNA pellet was dissolved in µl of loading dye (50 ml contains ml of 25 mM EDTA, pH8.0; 10 ml of deionised formamide; 50 mg Dextran blue and 39 ml of H2O) and ... for minutes and cooled in ice immediately µl each of dGTP, dTTP and dCTP with 7.5 µl of Random Primers Buffer Mixture and 2.5 µl of 32 P-dATP was added to make up to a total volume of 25 µl after...

Ngày tải lên: 03/10/2015, 20:57

... amplifications of RpCAbr and RpCAtr with 18S amplification The number of cycles on the y-axis is the difference between the number of cycles required to amplify each transcript and the number of cycles ... branchial plume cDNA with an open reading frame of 726 nucleotides and 5¢- and 3¢ UTR sequences of 171 and 442 nucleotides, respectively Positions of the primers on the complete cDNA are given ... previously sequenced RpCAtr (BPNJ ¼ 100 and BPMP ¼ 99) Fungia scutaria (FCA-a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences fall outside of clade I and are more closely related to each...

Ngày tải lên: 18/02/2014, 16:20

... analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgÆmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) dimethyl sulfoxide and incubated ... 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12 ... reduced and polymerized form of GOU (DHP-GOU) was synthesized as follows Acetone (50 mL) containing 0.2% GOU aO and 0.2% conyferyl alcohol, and 20 mL of 3% H2O2 were dropped into 430 mL of 100...

Ngày tải lên: 17/03/2014, 03:20

... ACF7, neural isoform 2, is a member of the dystonin subfamily and the beta-spectrin superfamily Fig Preparation of an anti-ISP36 serum (A) Expression of human His6-ISP36 in E coli and its purification ... NaCl Animals Animal care, housing and killing were in accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation ... Tween-20 for each, and detected using H2O2 and 3,3¢-diaminobenzidine This work was supported by a grant-in-aid from the Ministry of Education, Science, Sports and Culture of Japan and grants from...

Ngày tải lên: 30/03/2014, 20:20

... signals were band-pass filtered with a 5th order Butterworth filter with a cut-off frequency range of 5-100 Hz The low cut-off attenuates the effects of movement [30], while the high cut-off attenuates ... Alves and Chau Journal of NeuroEngineering and Rehabilitation 2010, 7:22 http://www.jneuroengrehab.com/content/7/1/22 Page of 10 Figure Signal denoising The microphone signal and RMS values of the ... data, and drafted the manuscript TC conceived the study, advised on the design and coordination of the experiments, and edited the manuscript All authors read and approved the final version of the...

Ngày tải lên: 19/06/2014, 08:20

... courtesy of the University of Bath, England, showed that the stress-strain profile of the unadulterated PU foam sample and that of the PPy-coated PU foams sample were similar showing regions of elastic ... Outside of medical applications, knowledge of the state of the body is essential in many wearable, mobile, and ubiquitous computing applications It is common in wearable and ubiquitous computing applications ... variety of body shapes and sizes would be hard to predict Similar issues would arise with sizing, fit, and sensor locations on the foot Because of the increased number of sensors and precision of...

Ngày tải lên: 19/06/2014, 10:20

... quantitatively significant product of FLSs [4] and little is known about the processing and function of the c19orf10 molecule Materials and methods Cells, tissues, and synovial fluid Synovial tissue ... rupole time -of- flight mass spectrometer [10], and proteins were identified by single MS (peptide mass fingerprinting) using ProFound [11] and by tandem mass spectrometry (MS/ MS) using Tandem search ... Expression of c19orf10 in OA synovium (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular...

Ngày tải lên: 09/08/2014, 10:20

... basis of our data this might be a consequence of the rapid conversion of CGA to DCQs as by means of an unknown downstream enzymatic step Indeed the involvement of the HQT gene in the profile of ... with the integration of the HQT and HCT genes The former increased the number of bridge markers on LG5, and reduced some large gaps (of 10 cM and cM) affecting the female and the male LGs (Fig ... Figure A simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in plants A simplified diagram of enzymes and major products in the synthesis of chlorogenic acid in...

Ngày tải lên: 12/08/2014, 03:20

... eluted and titered [Percentage of phage binding = (number of phage bound in the presence of peptide competitor/number of phage bound in the absence of peptide competitor) × 100] In vivo study of ... [5-8] Adamantane derivatives (amantadine and rimantadine) and neuraminidase inhibitors (NAIs; zanamivir and oseltamivir) are currently used for the chemoprophylaxis and treatment of influenza [9] ... virus to control and treat infections Traditionally, the generation of new drugs involves screening hundreds of thousands of components against desired targets via in vitro screening and appropriate...

Ngày tải lên: 12/08/2014, 04:21

... Figure contribution of unequal crossover and retroposition Relative Relative contribution of unequal crossover and retroposition 'TAG/Retro' is the ratio of the cumulative number of tandemly arrayed ... results of this study will further our understanding of the mechanisms of gene duplication in mammals Pan and Zhang R158.3 comment of the human genome [32], and usually not contain genes [33] Third, ... linear functions of which are Hn and Mn (red) The FAM2 gene set was plotted in bold lines, the linear functions of which are Hp1 and Mp1 (red) for the part with Ks ≤ 0.25, and Hp2 and Mp2 (green)...

Ngày tải lên: 14/08/2014, 08:20

... of action of bpV(pic) on spinal neurons, we treated injured spinal neurons in vitro with 100 nM bpV(pic) and confirmed its neurprotection and action through inhibition of PTEN and promotion of ... thorough overview of pathology and cell- and signal-specific mechanisms of survival and repair in a clinically relevant rodent SCI model Xiao-Ming Xu, Ph.D., Chair v TABLE OF CONTENTS List of Tables ... of inflammatory and foreign molecules and cells into the typically secluded parenchyma of the CNS (Tator and Fehlings, 1991, Casella et al., 2006) Currently, the involvement and interaction of...

Ngày tải lên: 24/08/2014, 09:14

... suspension with particle size of 100 and 150 nm and induction of apoptosis Ye HQ et al., College of Life Science and Technology, Huazhong University of Science and Technology, PRC Myeloid leukemia ... JZ and Ho PC, 2006] Inhibition of growth and promotion of apoptosis Observations of the clinical utility of realgar in the treatment of APL have triggered investigations into the mechanisms of ... pathway by up-regulation of surface expression of TRAIL and TRAIL receptors and activation of BID in cells expressing mutant p53 [Akay C et al., 2004] Induction of the release of mitochondrial AIF...

Ngày tải lên: 11/09/2015, 16:03

... Chromatograms of Arginine (a), hydrolysis product of soluble GRGDS peptide (b) and soluble galactose ligand (c) as standards, and hydrolysis product of the PET-Hybrid conjugated with GRGDS peptide and ... metabolic profiles and toxicity of hundreds of thousands of drug-like chemical compounds in order to screen out the promising candidates which can then enter the following animal testing and pre-clinical ... and drugs; (3) metabolism of fats, proteins, and carbohydrates; (4) enzyme activation; (5) storage of glycogen, vitamins, and minerals; (6) macromolecule and protein synthesis (i.e albumin, and...

Ngày tải lên: 12/09/2015, 08:19

... in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE ... humor, kindness, and help Thanks to all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks to all staff of Department of Clinical ... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like to express...

Ngày tải lên: 14/09/2015, 12:42