biology infections and laboratory diagnosis of chlamydia

... simulations of patients for palliative treatment of metastases since the soft tissue and bone windows are adequate Soft tissue and bone windows of MVCT scans have quite reasonable resolution and can ... shifts of > 10 mm, we observed only 7% and 2% in the directions of left-right and anterior-posterior but 13% in the direction of superiorinferior The median and maximum couch shifts are 4.2 and ... status of ≥ 60 and underwent stereotactic body radiotherapy planning and treatment between October 2008 and February 2011 as part of an institutional research ethics boardapproved study of neoadjuvant...

Ngày tải lên: 07/03/2014, 23:20

... The slope and R value of standard curves for other reactions are listed in Table Specificity of the qRT-PCR qRT-PCR specificities were assessed by melting curve analysis and sequencing of amplified ... and sequencing amplified PCR products Melting curve analysis and sequencing amplified PCR products of reactions for, Cb and Vb subgroups consisting of a single subgroup showed a single peak and ... sensitive, and highly reproducible This qRT-PCR method could also be used to characterize novel SAgs, to determine complete profiles of currently known SAgs, and to help understand the role of T cells...

Ngày tải lên: 18/06/2014, 16:20

... Detection limit and standard curve of the multiplex real-time PCR The detection limit and standard curve of the multiplex real-time PCR was determined using S Typhimurium ATCC 14028 and S Enteritidis ... the results of the standard culture method of isolated field and reference strains with those of the multiplex real-time PCR Results Specificity of the multiplex real-time PCR A total of 128 bacterial ... 3.57 ± 0.02 and 3.57 ± 0.03 log10 CFU/ml for S Typhimurium and S Enteritidis in beef, and 4.57 ± 0.02 and 2.26 ± 0.05 log10 CFU/ml for S Typhimurium and S Enteritidis in pork (Figs and 4) Our...

Ngày tải lên: 07/08/2014, 23:22

... series of dilution of 1-1010) of WEEV and EEEV and incubated at 37°C, and CPE was observed after days All virus isolations were performed in quadruplicate The titres of WEEV and EEEV were determined ... With the globalization of international trade, the rapid and free movement of large amounts of people, animals, food and feed products has created the risk of a novel and epidemiologically vulnerable ... a robust and valuable tool for highly sensitive and specific detection of WEEV and EEEV infection Page of 11 Lambert AJ, Martin DA, Lanciotti RS: Detection of North American Eastern and Western...

Ngày tải lên: 12/08/2014, 01:22

... modified protocols and the MF-MR and QMF-QMR primer sets described in Table New primers, QMF and QMR, were created based on high homology positions of sequences of Sphingomonas sp MD-1, and designed ... s, and polymerization at 72°C for 30 s The amplification reactions contained µL of extracted DNA, 10 pmol of each primer, 2.5 U of Ex Taq polymerase (Takara, Japan), µL of 10 Ex Taq buffer, and ... for 35 s; and for BACT1369F, PROK1492R, and TM1389BACT2 at 56°C for 35 s The amplification reactions required µL of extracted DNA, 10 pmol of each primer, 30 pmol of TaqMan probe, 25 µL of TaqMan...

Ngày tải lên: 05/09/2013, 10:15

... Lecturer of Chemical and Materials Engineering at the University of Auckland, New Zealand He received his BE (1986) and PhD (1993) degrees in Chemical and Process Engineering from the University of ... Associate Professor of Chemical and Petroleum Engineering at the University of Calgary from late 1998 to the end of 2005 He joined the University of Auckland in January 2006 He is a registered Professional ... Professional Engineer and a member of a number of professional societies His research is centred on the two major areas of process simulation and control, and process design and development – particularly...

Ngày tải lên: 01/01/2014, 17:44

... quarter of Wisconsin’s public secondary schools cannot/do not offer a single AP course • Of the remaining schools that offer AP courses, ¾ offer only one or two per year • RESULT: Talented and ambitious ... Benefits: UNIVERSITY OF WISCONSIN CAMPUSES • Enhance campus diversity initiatives and results by developing and expanding a pool of students whose preparation will enhance their desire and ability to ... teacher’s room, board, and tuition incurred during a four day AP Consortium professional development course that will be offered at the University of Wisconsin-Madison and at Lawrence University,...

Ngày tải lên: 15/01/2014, 15:59

... bel, named in honor of Alexander Graham Bell, is defined as the common logarithm of the ratio of two power, Px and Py In engineering applications, the most popular description of signal strength ... …A:34† Units of Power Power and energy calculations are important in circuit analysis Power is defined as the time rate of expending or absorbing energy, and can be expressed in the form of a derivative ... …A:28† ÀI and …I ÀI where t0 is a real number A.5 Vector Concepts Vectors and matrices are often used in signal analysis to represent the state of a system at a particular time, a set of signal...

Ngày tải lên: 25/01/2014, 19:20

... demultiplexing; framing and error-handling; parameter marshalling and demarshalling; and operation dis- software by allowing decoupled suppliers and consumers, asynchronous event delivery, and distributed ... role of a mediator between ibility of tightly coupled software can substantially increase consumers and suppliers The Event Channel manages object the effort and cost of integrating new and improved ... Event Service test runs and displays a timeline of supplier and consumer activity Figures 12 and 13 show timelines from multi-threaded and singlethreaded implementations of the Dispatching Module,...

Ngày tải lên: 19/02/2014, 18:20

... purposes and it is often not practical, and certainly never desirable, to generate these by hand The use of machine-readable sources of published dictionaries represents a practical and feasible ... be done with knowledge of the syntax of the grammar code system and the significance of punctuation and font changes For example, semicolons indicate concatenated codes and commas indicate concatenated, ... sense two of "marry" Thus, the system knows that further semantic analysis need only consider sense two of "persuade" and sense one and two of "marry"; this rules out one further sense of each,...

Ngày tải lên: 22/02/2014, 09:20

... through the study of these organisms often leads to advances in our general understanding of eukaryotes We report here on the observation of chaotic oscillations in a continuous culture of the budding ... inclusion of the second burst does not sensibly affect the overall mean and standard deviation of the interbeat intervals [IBI], but it does have a significant effect on the statistics of the individual ... circahoralian mode and faster components, up to the Nyquist limit, Tmin ¼ 2Dt ¼ 24 s [45] The recurrence at 13 h intervals of both the 40-min mode and of a rhythm with a period of $ is quite clear...

Ngày tải lên: 07/03/2014, 10:20

Ngày tải lên: 11/03/2014, 08:20

... protocols such as IRC commands within ICMP traffic, use of non-standard ports and protocol field values, etc All of these are indicative of different security events and are subject to further ... Management Protocol (SNMP) events and Quality of Service (QoS) data for system and network performance This data is most often used for performance and uptime monitoring and maintenance It can also ... and headed to, and in what direction the traffic is moving Common attributes include source and destination IP addresses, source and destination ports, and device interfaces Recent versions of...

Ngày tải lên: 14/03/2014, 22:20

... processing and statistical sentiment modeling informed by, and contributing to, an understanding of the cultural and political practices at work through the use of Twitter The System For accuracy and ... shows the number of positive and negative tweets about each candidate (right and left bars, respectively) in the last five minutes as an indicator of sentiment towards the candidates We chose ... analysis of the ongoing 2012 U.S presidential election We use the Twitter “firehose” and expert-curated rules and keywords to get a full 120 and accurate picture of the online political landscape...

Ngày tải lên: 16/03/2014, 20:20

... Lecturer of Chemical and Materials Engineering at the University of Auckland, New Zealand He received his BE (1986) and PhD (1993) degrees in Chemical and Process Engineering from the University of ... Associate Professor of Chemical and Petroleum Engineering at the University of Calgary from late 1998 to the end of 2005 He joined the University of Auckland in January 2006 He is a registered Professional ... Professional Engineer and a member of a number of professional societies His research is centred on the two major areas of process simulation and control, and process design and development – particularly...

Ngày tải lên: 01/04/2014, 10:58

... reverse transcribed and amplified in the presence of primers and probes specific for β-actin house keeping gene and genes encoding different cytokines Expression of IFN-γ and IL-2 genes was significantly ... expression of genes encoding cytokines and chemokines and anti HBsAg serum titers in vaccinated healthy donors Correlation Correlation between expression of genes encoding cytokines and chemokines and ... presence of a μg/ ml concentration of HBsAg Following addition of RNAlater, total cellular RNA was extracted, reverse transcribed and amplified by qRT-PCR in the presence of primers and probes...

Ngày tải lên: 18/06/2014, 15:20

... evaluated Calibration standard curve and linearity of dilution Due to the lack of a standard reference material, calibration standard curves were not evaluated for quantification of cellular IFNγ response ... be both accurate and precise with % recovery between 80–120% (analyst had a 123%) and % CV < 20%, respectively (Table 3) Calibration standard curve and linearity of dilution A standard curve was ... stimulated with irrelevant peptide and HIV had very low background signal and % CV was high, as expected Accuracy, spike and recovery, and LOQ Due to the lack of a reference standard material to establish...

Ngày tải lên: 18/06/2014, 15:20

... reaction contained µl of cDNA from the RT reaction, µl of 10× PCR Buffer, 1.5 mM MgCl2, µl of 10 mM each of dATP, dCTP, dGTP, and dTTP per reaction, µM each of primers and 2.5 units of Platinum Taq ... Sub-Programme (Dept of Agriculture and Food, Republic of Ireland) Thank you to Mr Noel Shanaghy and Mrs Breda Doody of Waterford Regional Hospital, Mr Eddie Beggan of Limerick Regional Hospital and Dr Jim ... Amplification of GII standards showing fluorescence versus cycle number concentration of × 107 – × 101 molecules are shown from left to right (B) Standard curve of GII assay R2 1.00 and a slope of -3.7...

Ngày tải lên: 18/06/2014, 18:20