biology and treatment volume 16

... of uPA gene expression [16] d Fig Effects of Decma treatment on E-cadherin distribution, cell scattering and uPA expression (A) T47D cells (a and b) and MCF7 cells (c and d) were treated for ... supernatant and immunostained with anti-E-cadherin IgG recognizing the cytoplasmic part of E-cadherin (B) T47D cells (a and b) and MCF7 cells (c and d) were grown for days to 60–70% confluence and then ... western analysis and immunostaining), and anti-phospho-Src (Tyr 416) polyclonal antibodies were obtained from Transduction Laboratories (Basel, Switzerland) Anti-phosphoPKB (Ser473) and anti-phospho-Erk...

Ngày tải lên: 19/02/2014, 05:20

... first reported the functional replacement of kaiBCp [15], and now Nakahira and coworkers [16] report the functional replacement of both kaiAp and kaiBCp Both studies found that expression of the Kai ... apparently all that is required is the expression of an Genome Biology 2004, 5:217 http://genomebiology.com/2004/5/4/217 Genome Biology 2004, Volume 5, Issue 4, Article 217 Johnson 217.3 KaiA comment ... all organisms http://genomebiology.com/2004/5/4/217 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Acknowledgements I thank Takao Kondo and Susan Golden for a productive and exciting collaboration...

Ngày tải lên: 09/08/2014, 20:20

... Decreased DNA binding and p65 protein level Decreased nuclear p50 and p65 levels and phosphorylated STAT1 and STAT5 Decreased IKKb and decreased p65 binding Increased IjBa expression and decreased p65 ... CHOP and Gadd 45 CHOP CHOP HIF-1a/2a, Gadd 34 and CHOP HIF-1a HSF-1 HSF-1 Reference Increased mRNA level Increased mRNA levels and increased c-jun activity Increased p53 phosphorylation and decreased ... mRNA level Decreased mRNA and protein levels Decreased HIF-1/2 a protein, increased Gadd 34 and CHOP mRNA levels Decreased protein level c-jun AP-1 (c-jun) and c-myc p53 and c-myc [123] [124] Increased...

Ngày tải lên: 07/03/2014, 00:20

... methylation and expression of genes encoding p27Kip1 and p18INK4c Proc Natl Acad Sci USA 102, 14659–14664 FEBS Journal 276 (2009) 162 9 164 0 ª 2009 The Authors Journal compilation ª 2009 FEBS 163 9 MLL and ... (2009) 162 9 164 0 ª 2009 The Authors Journal compilation ª 2009 FEBS B P Mishra et al 12 Ansari KI, Mishra BP & Mandal SS (2008) Human CpG binding protein interacts with MLL1, MLL2 and hSet1 and ... into the cytoplasm and coordinate in a similar fashion during the cell cycle FEBS Journal 276 (2009) 162 9 164 0 ª 2009 The Authors Journal compilation ª 2009 FEBS 163 1 MLL and H3K4 methylations...

Ngày tải lên: 23/03/2014, 06:20

... CH, and KT carried out the treatments and tissue collection, prepared preliminary data summaries, and participated in microarray analyses KE performed RNA extractions and microarray analyses, and ... K, Vecsei P, Runnebaum B, Rabe T: Cortisol and cortisone in human follicular fluid and serum and the outcome of IVF treatment Hum Reprod 2003, 18 :161 3 -161 7 Thurston LM, Norgate DP, Jonas KC, Gregory ... 90 and 180-day black and LY mice (mean Body weight (g) of 90 and 180-day black and LY mice (mean ± SEM; n = for each group) relative expression, i.e agouti was 350-fold greater in LY mice and...

Ngày tải lên: 20/06/2014, 07:20

... further into epimastigote and metacyclic forms within the insect The three recent BMC Genomics papers by Kabani and colleagues [1], Jensen and colleagues [2], and Queiroz and colleagues [3] have ... constitutive and that chromatin structure, in part mediated through histone modifications, will determine transcription start and termination sites These not seem to be sequencespecific and several ... between the bloodstream of mammalian hosts and the tsetse fly vector This cycling is accompanied by changes in morphology, in metabolism, and in RNA and protein expression Because the genome of...

Ngày tải lên: 06/08/2014, 19:21

... were: 30 cycles of 30 s at 94℃, 30 s at 60℃ and 90 s at 72℃ for β-actin, IL-1β and TNF α, 25cycles of 30 s at 94℃, 30 s at 56℃ and 30 s at 72℃ for IL-6 and IFNγ The sequences of primers used are ... splenocytes to 10-9M of TCDD induces IFNγ and TNFα gene expression, and suppresses IL-1 expression 10-6M phenol suppresses IL-1, IL-6 and TNFα gene expression, and 2-BP downregulates TNFα gene expression ... KK Cytokines (IL-1 beta, and TNF alpha) in relation to biochemical and immunological effects of 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) in rats Toxicology 1997, 116, 9 -16 Fujimaki H, Shiraishi...

Ngày tải lên: 07/08/2014, 15:20

... ihsigeN ,J rehtnyG ,R erooM ,P iksokaknoH 21 191-971 ,8 ,9891 AND seneg tnereffid morf debircsnart sANRm 054P owt fo noitaluger :ANDc 054P emorhcotyc elbicudni latibrabonehp nekcihc a fo ecneuqeS ... fo emulov noitcaer lanif a ni tuo deirrac saw RCP slocotorp eht gnisu tuo deirrac saw sisehtnys ANDc dnarts tsriF ANR fo noitartnecnoc lµ/gµ htiw detcudnoc saw RCP-TR evitatitnauQ RCP-TR evitatitnauQ ... eht sa tes saw snoitidnoc gnilcycomrehT lm 52 fo emulov lanif eht ekam ot ebut hcae ni dedda saw ANDc fo elpmas lm eerhT ebut RCP hcae ni seititnauq lm 22 ni detouqila dna deraperp saw selpmas...

Ngày tải lên: 07/08/2014, 18:21

... NM_000 416 IFNGR1 NM_002356 MARCKS Function Unknown AK001064 LOC86026 AK054 816 ORAOV1 NM_006470 TRIM16 AF245505 MXRA5 AL162069 LOC144501 BE884686 LTB4DH asparagine synthetase growth arrest and DNA-damage-inducible, ... 30], DNA repair (GADD45A and DDIT4) [31, 32], cell cycle and proliferation (ASNS and GADD45A) [33, 34], tRNA synthesis (WARS, GARS, YARS) [35-37] and transcription (SQSTM1 and DSIPI) [38, 39] The ... West Sacramento, CA) and incubated at 37˚C with 5% CO2 Iodine and estrogen treatments 24 hours prior to iodine treatment cells were grown in control medium consisting of RPMI 164 0 supplemented with...

Ngày tải lên: 08/08/2014, 17:20

... of chemokines and their receptors [10–12] Cytokines and chemokines in pSS In patients with pSS the salivary and lacrimal glands are the main target organs for the immune system and, at least ... in affected salivary glands of patients with pSS are IL-1, IL-6, IL-10, transforming growth factor (TGF)-β, interferon (IFN)-γ and TNF Fox et al [16] found that salivary gland CD4+ T cells produced ... normal salivary glands It has therefore been suggested that T helper type (Th1) cytokines, such as IFN-γ and IL-2, as well as IL-10, IL-6 and TGF-β, might be important in the induction and/ or maintenance...

Ngày tải lên: 09/08/2014, 01:21

... Q-PCR using the standard curve method The CEs were determined based on the PBMC reference standard Table presents the CEs of GAPDH, IL-1β, IL-6 and MMP-1 with the standard deviation and percentage ... detected using SYBR green technology and plasmid standards [24] However, each plasmid standard must be separately produced and validated Use of multiple primed plasmid standards is further confounded ... using RA and OA synovia To determine whether the Q-PCR technique can detect differences in cytokine gene expression in OA and RA synovia, nine RA and 13 OA synovial tissues were sampled and assayed...

Ngày tải lên: 09/08/2014, 01:23

... combination (TNF-α + EGF) and the levels of aggrecan and type II collagen mRNA were analyzed (Fig 2) Following 24 hours of treatment R130 with TNF-α, levels of aggrecan and type II collagen mRNA ... mRNA were decreased by 42 ± 4% and 39 ± 2%, respectively EGF alone decreased levels of aggrecan and type II collagen mRNA by 44 ± 5% and 42 ± 4%, respectively Treatment of chondrocytes with TNF-α ... of aggrecan and type II collagen mRNA (89 ± 2% and 81 ± 7%, respectively; data not shown) The combination of TNF-α and EGF therefore produces an additive decrease in both aggrecan and type II...

Ngày tải lên: 09/08/2014, 06:22

... primer, reverse primer and TaqMan probe for assay on demand and endogenous control β-actin and 12.5 μl of Taqman 2× Universal PCR Master Mix (Applied Biosystems) and the volume was made up with ... 12 hour light and 12 hour dark periods Rats had free access to standard food and water ad libitum All animal care and procedures were done in accordance with the Institutional and National Institute ... Insulin and curcumin treatment reversed the altered expression to near control (Figure and 2) Real Time-PCR Analysis of dopamine D2 Receptor in cerebral cortex and cerebellum of control and experimental...

Ngày tải lên: 10/08/2014, 05:21

... two treatments (OP-1 antisense and rhOP-1); and 3) gene ontology, when changes were analyzed within a family of genes according to their function (comparison was made between single treatment and ... trehalose, and 0.1% Tween 20), 0.5X SYBR Green (vendor stock 10,000X; Cambrex, Rockland, ME), 0.2 μM each of forward and reverse primer (IDT primers) or 1X QuantiTect primers (Qiagen primers) and μl ... activities (cytokines and their Genes down-regulated by rhOP-1 B A 99 110 83 83101 86 101 144 108 173 416 137 Binding Biological Process 161 418 130 93 103 Cellular Component 161 100 Cellular Process...

Ngày tải lên: 12/08/2014, 15:22

... correspond to unspliced (encoding Gag and Gagpol), singly spliced (to produce Vif, Vpr, Vpu and Env) or multiply spliced (for generating Tat, Rev and Nef) mRNAs Unspliced and singly spliced viral RNAs ... unspliced, singly spliced and nef mRNAs to block their translation by preventing the binding of PABP1 (shown as a small blue circle) In contrast, PABP1 binds to tat and rev mRNAs, and translation is ... reading frames and the influence of translation on 3' UTR structure/RNP composition Both tat and rev mRNAs contain reading frames encoding the respective proteins (Tat or Rev) and that of Nef,...

Ngày tải lên: 12/08/2014, 23:20

... (Dhp); and its subsequent hydroxylation, catalyzed by DOHH, yielding hypusine (Hpu) DHS is inhibited by GC7 and DOHH by CPX and DEF, as indicated B Structures of CPX, Agent P2, DEF and DFOX C CPX and ... from nt 1506 to 5784 using SpeI and SalI enzymes Similarly, truncations IV (nt 5784 to 8464) and V (nt 712 to 8464) were made with SaII and BamHI and with BssHII and BamHI, respectively The plasmid ... figures, and participated in writing the manuscript HMHA, PP and MBM initiated the project TP and MBM designed the experiments, analyzed the data, and wrote the manuscript HMHA analyzed the data and...

Ngày tải lên: 12/08/2014, 23:22

... content, which goes down to 2N and back to 4N and never repeats the 8N In between the start and the end there is no systematic variation of cells through G1, S and G2 In summary, the work of ... synchronization Cell Prolif 1998, 31:9 -16 Page of (page number not for citation purposes) Theoretical Biology and Medical Modelling 2005, 2:47 10 11 12 13 14 15 16 http://www.tbiomed.com/content/2/1/47 ... cell cycle, and during the period of cell division there should be a bimodal distribution of cell sizes One should apply clearly stated and stringent criteria for synchronization and microarray...

Ngày tải lên: 13/08/2014, 23:20

... peer-reviewed Genome Biology 2007, 8:212 212.4 Genome Biology 2007, Volume 8, Issue 5, Article 212 Koester and Insel publications would ensure the atlases continue to support user needs and the current ... looking to understand the role of individual genes in specific subsets of cells in the nervous system Genome Biology 2007, 8:212 http://genomebiology.com/2007/8/5/212 Genome Biology 2007, The ... user to several standard published adult and developmental brain atlases for both mice and rats The GENSAT project goes farthest in describing the anatomy of gene expression and compares data...

Ngày tải lên: 14/08/2014, 07:21

... plasma membrane and vesicular transporters for choline and acetylcholine (cho-1 and unc-17), GABA (snf-11 and unc-46, unc-47), dopamine (dat-1 and cat-1), and glutamate (glt-3 and eat-4) are included ... peptide expressed in ASIL and ASIR) [18] and gcy-8 (guanylate cyclase expressed in AFDL and AFDR) [19] (Figure 5) Volume 8, Issue 7, Article R135 R135.6 Genome Biology 2007, Volume 8, Issue 7, Article ... nca-1, and syg-1 in A-class (DA, VA) and in other ventral cord motor neurons (for example, DB, VB) Genome Biology 2007, 8:R135 http://genomebiology.com/2007/8/7/R135 Genome Biology 2007, Volume...

Ngày tải lên: 14/08/2014, 07:22