biochemical and molecular approaches

Studies on the mechanisms of the beneficial effects of herba leonuri and leonurine on traumatic brain injury in rat

Studies on the mechanisms of the beneficial effects of herba leonuri and leonurine on traumatic brain injury in rat

... loss, astrocyte and microglia gliosis following TBI 61 3.1.2 Biochemical and molecular approaches 67 viii 3.1.2.1 Effects of pHL on the activities of SOD, CAT, GPx and GST in the ... through antioxidant and anti-apoptotic mechanisms 71 3.2.1 Biochemical and molecular approaches 71 3.2.1.1 Effects of Leo on the activities of SOD, CAT, GPx and GST in the cortex ... activated and secreted (Shohami et al., 1994a; Taupin et al., 1993) These processes induce chemokines and adhesion molecules and subsequently recruit immune and glial cells in a parallel and synergistic...

Ngày tải lên: 02/10/2015, 17:15

154 723 0
báo cáo hóa học:" Oxford unicompartmental knee arthroplasty: medial pain and functional outcome in the medium term" pptx

báo cáo hóa học:" Oxford unicompartmental knee arthroplasty: medial pain and functional outcome in the medium term" pptx

... the femoral component and the Oxford score (0.38) Examples of good and poorly positioned prosthesis can be seen in Figures and Edmondson et al Journal of Orthopaedic Surgery and Research 2011, ... implantation and could not be traced The remaining 38 patients responded to a postal and telephone enquiry using the Oxford Knee Score functional questionnaire [14] - where is the worst score and 48 ... condyle, and one was revised because of persitent and unexplained medial pain, in all cases symptoms resolved with conversion to TKA We found little correlation between component malpositioning and...

Ngày tải lên: 20/06/2014, 07:20

7 345 0
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural Genomics ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... PRTFDC1 (A) monomer and (B) tetramer generated using symmetry-related molecules Interactions with GMP (C) GMP, phosphate and calcium ions are shown as sticks and spheres colored pink and black, respectively...

Ngày tải lên: 15/02/2014, 01:20

11 770 0
Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... (a helix signature), 218 nm (b strand signature), 202–204 nm (random coil signature) In WhiB5 and WhiB6, the structure was dominated by a helices and b strands and the proportion of these structural ... report the biochemical and biophysical properties of WhiB2, WhiB5, WhiB6 and WhiB7 of M tuberculosis and also compare the properties of all seven WhiB proteins We show that, similar to WhiB3 and WhiB4, ... S1 and S2) The presence of four conserved cysteines and the brownish appearance of purified WhiB1, WhiB2, WhiB5, WhiB6 and WhiB7 indicated the presence of an iron–sulfur cluster To identify and...

Ngày tải lên: 18/02/2014, 12:20

18 548 0
Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

... between R81 and P82 and between G90 and I91 A similar cis peptide between G93 and V94 is found in Tm SurE also Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... stable than its homologues and an attempt was made to understand the molecular basis of the lower thermal stability 5856 Results and Discussion Preliminary characterization and crystallization of ... archaic and thermophilic homologues The St SurE monomer consists of 13 b-strands, six a-helices and three 310-helices The core of the protein is made up of a nine-stranded b-sheet flanked by a1, a5 and...

Ngày tải lên: 18/02/2014, 14:20

10 554 0
Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf

Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf

... performed with rabbit preimmune and post-third inoculation sera against a M tuberculosis sonicate (lanes 1, and 5) and a membrane fraction (lanes and 4) Lanes and 3, absence of recognition of ... recognizing a 33 kDa molecular mass band in the M tuberculosis sonicate, close to the protein’s molecular mass (33.1 kDa) in lane Preimmune serum (lane 3) did not recognize bands in the membrane ... nm) (Fig 5A) Scatchard and Hill analyses were then performed and dissociation constants (Kd) were calculated for Rv2560 HABP 11024 for U937 and A549 cell lines (Kd of 380 and 800 nm, respectively)...

Ngày tải lên: 18/02/2014, 16:20

13 492 0
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of the model was ... in green, and the cluster of hydrophobic residues Trp390, Trp393, and Trp394 is in red The catalytic site is enlarged in B and illustrates the conserved disulfide bridge between Cys239 and Cys216 ... gradient (broken line), and concentrations of LPL (j) and lipase activities (h) were measured in each fraction as described in the Materials and methods The upper, middle, and lower panels are the...

Ngày tải lên: 21/02/2014, 15:20

10 680 0
Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... sPPase (not shown) and P 6903 sPPase (supplementary Fig S1) (native molecular masses of 114 ± kDa and 120 ± kDa, respectively) In all cases, both the subunit molecular masses and oligomeric states ... UÆmg)1 protein and recovery levels of 20– 30% In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20–22 kDa (Figs and 2; supplementary ... both cases The positions and molecular masses of protein standards are indicated on the left side of the gels (A) The upper right inset shows column calibration protein standards (CAT, catalase;...

Ngày tải lên: 16/03/2014, 11:20

12 415 0
báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt

báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt

... of bands after background subtraction and are relative to white (value 0) and black (value 256) The blots each show a single band for Akt and p-Akt at about 60 kDa and each show a double band ... double band for Erk and p-Erk at about 44 and 42 kDa Experiment Effects of LH in combination with PD98059 and/ or LY294002 on cell number and secretion of androstenedione and progesterone from ... gonadotrophins and IGF with the Akt and Erk signalling pathways in theca and granulosa cells in vitro and to describe their functional significance for ovarian follicle growth in vivo Materials and methods...

Ngày tải lên: 20/06/2014, 07:20

13 438 0
Báo cáo y học: "Functional outcome of nerve transfer for restoration of shoulder and elbow function in upper brachial plexus injury" potx

Báo cáo y học: "Functional outcome of nerve transfer for restoration of shoulder and elbow function in upper brachial plexus injury" potx

... the main operating surgeons and designed the study, PB and SRJ performed data collection and analysis of the results, HR and PB were involved in sequence alignment and drafting of the manuscript, ... normal use of the upper extremity It improves the working space of the hand and makes the hand more useful Shoulder stability and abduction are frequently accomplished by arthrodesis of shoulder ... stabilisation remain denervated [7] Allieu and Cenac [4], Narakas and Hentz [1], Thomeer and Malessy [8] have reported satisfactory function in 0%, 36% and 50% respectively Chuang et al [9] have...

Ngày tải lên: 10/08/2014, 10:20

9 267 0
Báo cáo y học: "Combination of histopathological and electromyographic patterns can help to evaluate functional outcome of critical ill patients with neuromuscular weakness syndromes" docx

Báo cáo y học: "Combination of histopathological and electromyographic patterns can help to evaluate functional outcome of critical ill patients with neuromuscular weakness syndromes" docx

... immobility and antibiotics have been considered [7] Patients with weakness acquired in the intensive care unit (ICU) are often sedated and mechanically ventilated, and have unreliable sensory and motor ... ventricular dysfunction and type of surgery Organ failure score and presence of SIRS were noted, according to criteria presented by Bone and coworkers [13], at admission and during the clinical ... postfixed with 1% osmic acid and embedded in araldite Semithin resin transverse and longitudinal sections were stained using haematein and eosin, solochrome blue and paraphenylene diamine Ultrathin...

Ngày tải lên: 12/08/2014, 20:20

9 427 0
Báo cáo y học: "Retrospective agreement and consent to neurocritical care is influenced by functional outcome" ppt

Báo cáo y học: "Retrospective agreement and consent to neurocritical care is influenced by functional outcome" ppt

... contributions ICK and HBH designed the study and wrote the manuscript ICK, JBK, LB, and CM obtained clinical data by reviewing institutional databases and the patient’s medical charts ICK and MK obtained ... treatment and functional outcome is less conclusive Although Foerch and colleagues [16] reported a correlation between the retrospective decision against treatment and rather unfavorable outcome, and ... (GBS) and myasthenia gravis (MG); 7, neurodegenerative diseases and encephalopathy; 8, cerebral neoplasm; and 9, intoxication) Patients or their relatives were contacted by using a mailed standardized...

Ngày tải lên: 13/08/2014, 21:21

7 299 0
Multi manifold diffeomorphic metric mapping for cortical registration with its applications in brain structural and functional studies

Multi manifold diffeomorphic metric mapping for cortical registration with its applications in brain structural and functional studies

... where αi (t) and xi (t) satisfy the geodesic shooting equations (3.5) and (3.6) nc and n s are i i numbers of curves and surfaces incorporated in the mapping Eγi (ϕ1 · γtemp , γtarg ) and ES i (ϕ1 ... regions of interest in the lateral and medial views, respectively The superior frontal region, precentral and postcentral region, temporal region, and parietal and occipital region are respectively ... curves and twelve gyral curves are illustrated in the lateral (panels A, D), medial (panels B, E), and basal (panels C, F) views of the template surface The top and bottom rows show sulcal and gyral...

Ngày tải lên: 09/09/2015, 18:53

137 520 0
Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus

Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus

... grade and were purchased from Sigma and Merck companies, unless otherwise stated 2.1.1 Enzymes and other proteins Enzymes for molecular biology were mainly purchased from New England Biolabs, and ... Canhua, Dr Wu Jinlu, Ms Tang Xuhua, Ms Sunita and, Mr Jobi and the rest of the lab mates for the valuable discussion and friendship and the present and former members of Functional Genomics Laboratory ... rupturing the host cell and eventually killing the host cell before infecting other cells (Emiliani, 1993) Viruses are ubiquitous and abundant in nature and can infect and parasitize all living...

Ngày tải lên: 14/09/2015, 09:57

148 397 0
Structural and functional studies on type III and type VI secretion system proteins

Structural and functional studies on type III and type VI secretion system proteins

... including grlA and grlR, are positively regulated by Ler, and Ler expression is in turn positively and negatively modulated by the proteins GrlA and GrlR, respectively However, the molecular basis ... (bubonic, pneumonic, and septicemic)(Y pestis), enterocolitis and mesenteric lymphadenitis (Y enterocolitica and Y pseudotuberculosis) Enterocolitis in humans and typhlitis and typhoid-like disease ... Rosenshine and Dr Henry Mok for helping in manuscript preparation I am also thankful to Dr Li Mo, Dr Zheng Jun, and Gal Yerushalmi for all their help I would like to thank Dr Anand Saxena and Dr...

Ngày tải lên: 14/09/2015, 14:13

169 357 0
Depression, cognitive impairment and physical functional outcome and their associated factors in stroke patients in community hospital care

Depression, cognitive impairment and physical functional outcome and their associated factors in stroke patients in community hospital care

... thanks and gratitude to my supervisor, Dr Fong Ngan Phoon for his support and advice in conducting the research study and in the preparation of the dissertation My heart felt gratitude and thanks ... of stroke is around 2-2.25 per thousand population and a total prevalence rate is around per thousand population.1 It has been estimated that one in four men and nearly one in five women aged 45 ... regression to achieve the following, viz eating, grooming, and dressing the upper body, continence in bladder and bowel and transfer between a bed and chair with supervision only They found that this...

Ngày tải lên: 04/10/2015, 10:24

107 263 0
Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"

Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"

... target and novel visual stimuli in children of alcoholics Alcohol 1998; 15:119-36 92 Porjesz B And Begleiter H Human brain electrophysiology and alcoholism In: Tarter R and Thiel DV, Eds Alcohol and ... both active and passive P300 latency and age; in this same study, levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72] ... 11:141-151 67 Polich J, Hoffman D P300 and handedness Psychophysiology 1988; 35:497-507 68 Hopper KD, Patel S, Cann TS, et al The relationship of age, gender, handedness, and sidedness to the size of the...

Ngày tải lên: 02/11/2012, 11:08

8 563 0
Tài liệu Physical health and functional ability of an elderly, population in Sri, Lanka doc

Tài liệu Physical health and functional ability of an elderly, population in Sri, Lanka doc

... lower extremities were carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full) A younger ... injury or an illness Best performance was in "standing from chair without using arms" (84%), semi tandem stand was completed by 78% and full tandem stand by 72% The poorest performance was in shoulder ... in Myanmar, 84% in Indonesia and 62% in Thailand (10) It was D N Fernando and R de A Seneviratna 14 Table Self-assessed "health status" by some psychosocial factors and indicators of use of health...

Ngày tải lên: 14/02/2014, 06:20

8 462 0
Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands) ... intermediate levels in heart and placenta, and the lowest levels in spleen, kidney, liver and lung The transcript was not detectable in brain, thymus, muscles, small intestine and colon (Fig 3A) The ... within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and MCPIP (bottom panel)...

Ngày tải lên: 18/02/2014, 14:20

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