bioavailability bioequivalence and controlled release preparations

... estimated the initial value of parameters A, k and m as 3.9, 0.5 and 0.3, and 17, 0.9 and 0.7, and 3.0, 0.7 and 0.5 for D, H and V, respectively, and used the Gauss-Newton iterative method to ... (no controlled release fertilizer added), and FR-0.8, FR-1.6, and FR-3.2 to represent the 0.8, 1.6 and 3.2 g per seedling of the FR fertilizer treatments The moderate release (MR) and slow release ... incorporating controlled- release fertilizer in the root plug, the release characteristics and application rate of controlled- release products must be well matched to the size of the containerized stock and...

Ngày tải lên: 08/08/2014, 01:22

... enzyme treatment or thermocycling to exert controlled release of the USPIO cores Light scattering and relaxation measurements were carried out on clustered and declustered MRS in aqueous solution ... AL) and used as purchased The restriction enzymes EcoRI and EcoRV were purchased from New England Biolabs (Ipswich, MA) Custom ssDNA sequences designated C1 (5’amino-ACGTACGTGATATCTGCATGCA-3’) and ... details Department of Biomedical Engineering, Vanderbilt University; Nashville, Tennessee, USA 2Vanderbilt Institute for Nanoscale Science and Engineering, Vanderbilt University; Nashville, Tennessee,...

Ngày tải lên: 11/08/2014, 00:23

... Hubbell4 and Todd D Giorgio1,2* Affiliations: Department of Biomedical Engineering, Vanderbilt University; Nashville, Tennessee, USA Vanderbilt Institute for Nanoscale Science and Engineering, Vanderbilt ... Correction: Enzymatic- and temperature-sensitive controlled release of ultrasmall superparamagnetic iron oxides (USPIOs) Shann S Yu1,2, Randy L Scherer2,3, Ryan A Ortega1,2, Charleson ... Vanderbilt University; Nashville, Tennessee, USA Integrative Biosciences Institute, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland Email: SSY : shann.yu@vanderbilt.edu RLS: randy.l.scherer@vanderbilt.edu...

Ngày tải lên: 11/08/2014, 08:20

... the guidance and the help of several individuals who in one way or another contributed and extended their valuable assistance in the preparation and completion of this study First and foremost, ... Chapter Hypothesis and Objectives 47 2.1 Thesis rationale and hypothesis 47 2.2 Objectives 48 Chapter Carbon Nanotubes for Incorporation, Storage and Release of Cisplatin ... Chemical Reduction and Hydrophobicity Reversal 138 6.1 Introduction 138 6.2 Hypothesis and objectives 142 6.3 Materials and methods 142 6.3.1 Chemicals and reagents ...

Ngày tải lên: 08/09/2015, 18:10

... gratitude and appreciation for their support and encouragement over the years of my study And my special thanks and appreciation to my wife and my daughter for their encouragement and patience ... achieved by time -controlled and/ or target -controlled 22 INTRODUCTION delivery of drug In time -controlled delivery, the drug is released in a pre-determined rate while in target -controlled delivery, ... 1995) C3 Drug release mechanisms of controlled release delivery system Drug release mechanisms depend on the properties of the polymeric carrier, drug and additives present The release of drug...

Ngày tải lên: 13/09/2015, 20:39

... the undesirable release profile of drugs For nanoparticle and microparticle, a burst release at an early stage together with a very short release course is the major weakness Xie and Wang reported ... but the effective stage can not sustain (Xie and Wang, 2005) For disc and film, their release profiles are difficult to tailor and in most cases their release rate is too low (Jackson et al., 2004) ... (Conwell and Huang, 2005; Schreier, 1994; Chapter Tomlinson and Rolland, 1996) There have been two major approaches proposed: the viral mediated and non-viral mediated gene transfection (Ledley and...

Ngày tải lên: 14/09/2015, 08:41

... 30 days of release F4 seems less integrated and this finding indirectly explains why BMP-2 in F4 was released much faster than all other fibers Chapter 52 Controlled and sustained release of ... Lane 4: BMP-2 released from scaffold F3; Lane 5: BMP-2 released from scaffold F4 Chapter 54 Figure 3.9 A comparison of α-helix proportion of native BMP-2 ( ) and BMP-2 released from released from ... analysis; WBMP-2, WPLGA and WHAp are the weights of BMP-2, PLGA and HAp used in the scaffolds fabrication process respectively Chapter 40 3.3.3 In vitro release studies The in vitro release of BMP-2...

Ngày tải lên: 14/09/2015, 08:41

... segmental bone defects Aebli and colleagues (Aebli et al., 2005) and Saito and colleagues (Saito et al., 2005) reported that BMPs improve bone regeneration in vivo, and BMP-2 has been found to ... different As a result, P and τ have multiple solutions From Table 4.2, one can see that τ values for all scaffolds and control are all between and 1, which means that BMP-2 and ALP curves for all ... 76 weeks, F2 slows down and F1 catches up to the same score as F3 and F4 At the week 6, all bone defects have healed perfectly for scaffolds F1, F2 and F4, while control and F3 are only partially...

Ngày tải lên: 14/09/2015, 08:41

... from the in vitro release test, about 60% (40, 60 and 85% for A1, A2 and A3 respectively), 30% (18, 31 and 50% for B1, B2 and B3 respectively) and 16% (8, 16 and 24% for C1, C2 and C3 respectively) ... bursts of release and their release rates in the initial stage (cumulative release < 80%) are much lower than those of group A Furthermore, the release curve shows better sustained release than ... proportion of PLGA has degraded Compared with the release periods of groups A and B, group C scaffolds shows much longer release course and 95% cumulative release of DNA can be reached in 45-55 days,...

Ngày tải lên: 14/09/2015, 08:41

... different release properties of groups A, B and C, and are consistent with the in vitro release profiles of BMP-2 plasmid from different scaffolds (Nie and Wang, 2007), As naked Chapter 119 DNA and ... low transfection efficiency of naked DNA, and thus the low expression of naked plasmid released from A1 and A3 Consequently, the serum BMP-2 levels of A1 and A3 did not improve significantly after ... the sonication of organic and aqueous mixture Table 6.1 Chapter 114 summarizes the composition of the emulsion of the groups (A, B and C) and samples (A1-A3, B1-B3 and C1-C3) The detailed fabrication...

Ngày tải lên: 14/09/2015, 08:41

... type of release profile is typical for PLGA-based delivery devices, such as microparticles and microfibers (Xie and Wang, 2007; Nie and Wang, 2007) In contrast, the release profiles of F1 and F2 ... like F1 and F2 In this case, scaffolds F1 and F2 were able to release plasmid DNA until day 51 and day 60, respectively The electrophoretic mobility analysis of the DNA released from F0, F1 and F2 ... (Onishi and Machida, 1999) extracted from crustaceans and has been shown to be non-toxic in animals (Rao and Sharma, 1997) and humans (Aspden et al., 1997) Due to its good biocompatibility and cytotoxicity...

Ngày tải lên: 14/09/2015, 08:41

... small release bursts observed for the case of F1 and F2, each burst release was followed by a period of slow release profile Compared with the release profile of F0, the release profiles of F1 and ... in controlled release of DNA, but the release rate of DNA and subsequent expression of target protein is too low, especially in the initial stage (Figures 7.5a and 7.7b) Therefore, an initial and ... cumulative release curves for F0, F1 and F2 (mean ± S.D., n=3) (b) Electrophoretic mobility analysis of naked DNA and in vitro samples released after days All samples were run on a 1% agarose gel and...

Ngày tải lên: 14/09/2015, 08:41

... disintegration of the scaffolds, and subsequently enhance the release of BMP-2 protein from scaffolds, as shown in the in vitro release profiles (Figures 3.6 and 5.5) Animal experiments demonstrated ... advantage of polymeric particles and reduce immunological effect at the same time, a new DNA release system is developed which makes possible sustained DNA release and efficient transfection with ... helped improve the formation of new bone and the healing of segmental defects in vivo Groups A, B and C released DNA or DNA nanoparticles in different ways and time frames (Chapter 5), thus their...

Ngày tải lên: 14/09/2015, 08:41

... used to encapsulate and release both water-insoluble and water-soluble drugs In Chapter and Chapter of the present thesis, polymeric microspheres were used for the controlled release of an anticancer ... encapsulate bioactive agents and release them in a controlled manner The controlled Ruan Gang: PhD thesis Chapter Literature Review ──────────────────────────────────────────────── release behavior of ... high molecular weight proteins and low molecular weight hydrophobic drugs can be loaded and released The release rate is controllable by the initial drug loading and the polymer concentration...

Ngày tải lên: 17/09/2015, 17:20

... 4-(2-aminoethyl)bezenesulfonylfluoride/HCl (1 mM) and NaF (10 mM) and centrifuged for and 600 g at °C Nuclei were resuspended in extraction buffer three more times to fully lyse the nuclear envelope and complete extraction ... combined and yielded nucleosolic proteins The remaining pellet contains all DNA and structure bound proteins and is further referred to as chromatin-fraction Electrophoresis of proteins and Western ... Immunofluorescence staining of total cellular and chromatin-bound PCNA T24 were grown and incubated on coverslips Two sets of hypoxic cells and of cells reoxygenated for 10 and 30 were prepared One set of...

Ngày tải lên: 21/02/2014, 00:20

... Using SQL Trace and TKPROF Understanding SQL Trace and TKPROF Understanding the SQL Trace Facility Understanding TKPROF Using the SQL Trace Facility and TKPROF ... cost-based optimizer and describes how to gather and use statistics Chapter 4, "Understanding Indexes and Clusters" This chapter describes how to create indexes and clusters, and when to use them ... under a license agreement containing restrictions on use and disclosure and are also protected by copyright, patent and other intellectual and industrial property laws Reverse engineering, disassembly...

Ngày tải lên: 07/03/2014, 23:20

... we report on the measurement and modelling of CNT-induced cell movement Mammalian cells cultured with MWCNTs were tracked and studied for days in order to document and measure their migration ... identified in the well and their exact position determined at 0, 24, 48 and 72 h after placement of the magnet Figure compares the displacements of the cells treated with the CNTs and exposed to the ... one bond broken for each elementary displacement) and s0 = 10-8 s The overlap between the model and experimental data is quite good (R2 = 0.967), and the parameters are in the range of values referred...

Ngày tải lên: 22/06/2014, 00:20

... the most dormant stage obtained There are no strict borderlines to phases before and after deep dormancy Early and late phases of dormancy are characterized as pension structure of viable containing ... breaking treatments of and wk had a dramatic influence, hastening the tilushing rate, especially in the plants with lowest degree of dormancy In Fig the same reactions to temperature and break of dormancy ... Umeil, Rep 6, pp 81-98 Dormling I., Eriksson G & Jonsson A (1977) Photo- and thermoperiodic reactions of Pinus sylvestris and Pinus contorta (Swedish with English summary) In: Experimental Genecology,...

Ngày tải lên: 09/08/2014, 02:21

... the release of (pro-) inflammatory and angiogenic mediators by cultured autologous FLS from inflamed joints of RA and AC patients Materials and methods Patients Paired synovial fluid, plasma and ... manuscript, guided by RN and AS, with clinical input and final correction by PT RB, RN and AS devised the experimental design The selection of patients and collection of synovial biopsy and blood materials ... blood and synovial fluid by centrifugation for 20 at 1,550 g and 20°C For all determinations, aliquots of cell-free plasma and synovial fluid were snap-frozen in liquid nitrogen for at least 15 and...

Ngày tải lên: 09/08/2014, 06:22

... contributions SS designed and carried out PLGA formulation and characterization studies, data analysis and statiscal analysis, and drafting of the manuscript RS carried out animal injections and immunohistochemistry ... polyplex loaded and unloaded groups for 50 kDa and 17 kDa PLGA (C) Encapsulation efficiency (EE) of unloaded nanospheres and PEG-PEI-AO loaded nanospheres Non-endcapped 50 kDa and 17 kDa PLGA ... The nanometer size, charge shielding, and controlled release properties of the PLGA carriers should offer significant improvement in the biodistribution and sustained delivery of AO complexed...

Ngày tải lên: 11/08/2014, 00:22