bioassay of industrial waste pollutants

... Contributors Implementation of Industrial Ecology for Industrial Hazardous Waste Management Lawrence K Wang and Donald B Aulenbach iii ix Bioassay of Industrial and Hazardous Waste Pollutants Sveltana ... Rights Reserved Bioassay of Industrial Waste Pollutants 37 Figure Proposed ecotoxicological procedure for assessment of solid waste toxicity and calculation of the classes of hazard calculate ... posed by pollutants to the growth and survival of plants); (d) sentinel Copyright #2004 by Marcel Dekker, Inc All Rights Reserved Bioassay of Industrial Waste Pollutants 29 (monitoring the pollutants...

Ngày tải lên: 11/08/2014, 17:22

... different effect of tert-butanol on hydrogen peroxide decomposition and dye removal The presence of tert-butanol caused only slight change of hydrogen peroxide decomposition, whereas 10 mM of tert-butanol ... reaction rate of H2O2 with hydroxyl radical is relatively slow (k= 2.7x107 M-1s-1, Buxton et al (1988)) The relative difference of the respective rate constant of reaction make a decision of OH radical ... dye+OH • [OH •]ss − (1) A plot of observed first order rate constant (min-1) for dye removal as a function of FeOOH concentration is shown in Figure All R2 for the fitness of observed first order reaction...

Ngày tải lên: 05/09/2013, 08:40

... the role of peroxidase in the early stages of melanogenesis, despite reports of the involvement of peroxidase–H2O2 systems in later stages during the oxidation of indolequinone precursors of eumelanin ... tyrosinase-mediated reactions M Mastore et al Fig Overview of the principal melanotic pathways and some of the proposed sites of activity of DOPA decarboxylase (DDC), tyrosinase (TYR), tyrosine-3-hydroxylase ... of cells to either exogenous H2O2 [8,11], or to various reactive intermediates of oxygen followed by inhibition assays Observations of enhanced enzyme-mediated oxidations following exposure of...

Ngày tải lên: 07/03/2014, 17:20

... polyacrylamide) separation of the active fraction in (B) Approximately lg of protein was loaded in each lane Lane 1: CM prepared by incubation of 2000 ASGs Lane 2: upper phase of ultrafiltration of the CM Lane ... programmed cell death expressed with a PCD score of 0–6, where a score of indicates no change, and a score of signifies completion of PCD with the formation of apoptotic bodies [4] Nuclear morphology ... incubating one V5 ASG in 0.3 mL of Grace’s medium for 48 h (V5 CM) When the CM of one gland had less than unit of the activity, the number of glands cultured in 0.3 mL of medium was increased to five...

Ngày tải lên: 15/03/2014, 00:20

... function of the characteristics of the feed solution (type and concentration of organics, pH, presence of inorganic ions), the concentration of oxygen in the reaction solution, the magnitude of the ... disk angular velocity (ω) of rpm, solution ionic strength as KNO3 of 10 mM, solution volume in the reaction vessel (VR ) of 3.5 l, and influent concentration of 4-CBA (Cin ) of 300 ␮mol l−1 The incident ... effect at higher concentrations A summary of the reaction rate constants of certain of these reactions is provided in Table Redox potentials of many of these reactions in homogeneous solutions...

Ngày tải lên: 16/03/2014, 00:07

... forms of caspase-3 and a cleaved fragment of PARP were detected in cells treated with H2O2 Furthermore, overexpression of Hsp105a or Hsp105b markedly reduced both the amount of the active forms of ... cleavage of distinct and vital proteins [21–23] In particular, caspase-3 is one of the key executioners of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of ... overexpression of mouse Hsp105a or Hsp105b was induced by removing DOX from the culture medium In HeLa-tet ⁄ Hsp105a cells, the level of Hsp105a was increased approximately twofold by removal of DOX...

Ngày tải lên: 16/03/2014, 06:20

... activities of the chimeric APX samples per absorbance of Soret peak (roughly corresponding to heme amount) were 60–80% of that of APX-B, and that of the spinach stromal APX sample was 140% of that of ... Kitajima et al Inactivation of stromal ascorbate peroxidase Fig Alignment of amino acid sequences of C-terminal half-regions of ascorbate peroxidase (APX)-B and stromal APX of spinach Recombination ... amount of hydrogen peroxide, produced from oxygen by auto-oxidation of the remaining ascorbate, reacts with APX [5] and leads to inactivation of the stromal APX This property has the advantage of...

Ngày tải lên: 16/03/2014, 14:20

... FE-SEM micrograph of the assynthesized SiNWs The voluminous quantities of SiNWs were grown for h at 400 °C Fig XRD patterns before and after hydrogen radical treatment (a) Peaks of the asdeposited ... image of the as-fabricated indium nanocrystals as metal catalysts after hydrogen radical treatment Fig Top-view FE-SEM image of the SiNWs The inset shows a high-magnification FE-SEM image of the ... approximately 10 nm-thick amorphous outer layer The SAED pattern of one individual SiNW is shown in the inset of Fig The set of diffraction spots visible in the SAED pattern reveal that this...

Ngày tải lên: 16/03/2014, 15:13

... coefcient of the dimeric product of the reaction This e322 value was obtained from a plot of absorbance versus number of moles of hydrogen peroxide consumed in the HRP-catalyzed oxidation of 4, where ... times of 9.9, 10.4, 11.8, 12.0, 12.2 and 12.4 Fig Biphasic behavior of the rate of HRP-mediated phenol nitration as a function of nitrite concentration Rate dependence of HRP-catalyzed nitration of ... expansion of the plot in the low nitrite concentration range Fig Inhibition of the HRP-mediated phenol nitration by nitrite Plot of the rate of HRP-catalyzed nitration of phenol as a function of nitrite...

Ngày tải lên: 16/03/2014, 16:20

... degradation of MP8 had occured under those oxidative conditions (data not shown) The effect of changing the pH of the reaction medium on the efciency and selectivity of the reaction of in the presence of ... the addition of catalase Analysis of the reaction mixtures by RP-HPLC using UV-detection revealed a signicant decrease of the amount of starting N-hydroxyguanidine and the formation of three new ... MP8 selectively catalyses the oxidation of to the corresponding cyanamide with formation of only minor amounts of urea Reaction of and NOHA in the presence of MP8 and H2O2 leads to NO formation...

Ngày tải lên: 17/03/2014, 09:20

... the presence of malate plus glutamate, also maximized by the addition of rotenone as a result of the full reduction of FMN and of NAD+ Addition of glutamate allows the production of a-ketoglutarate, ... and cofactor reduction over the entire range of measured NAD(P)H autofluorescence By contrast, control mitochondria showed a marked lack of responsiveness in H2O2 efflux over a large range of cofactor ... and regulation of mitochondrial ROS production [10–18] The basic premise of the assay is straightforward Superoxide is the initial product formed as a result of the reduction of O2 by single...

Ngày tải lên: 22/03/2014, 21:21

... carcinogens, catechol enhanced the incidence of papillomas of the tongue, carcinomas of the oesophagus, squamous-cell carcinomas of the forestomach and adenocarcinomas of the glandular stomach 5.4 Other ... only when mammary tumours of all types were combined No increase in neoplasia was seen in hamsters 5.4 Other relevant data The observation of excretion of mercapturates of chloroprene indicates ... polymers Widespread exposure occurs during the production and industrial use of dichloromethane and during the use of a variety of consumer products containing dichloromethane Substantial losses...

Ngày tải lên: 28/03/2014, 19:20

... number of aspects of design and analysis as described in the report of the study Most of these considerations apply equally to case–control, cohort and correlation studies Lack of clarity of any of ... reassessment of the strength of the evidence If the risk of the disease in question increases with the amount of exposure, this is considered to be a strong indication of causality, although absence of ... animal, the dose of the carcinogen and the route and length of exposure Evidence of an increased incidence of neoplasms with increased level of exposure strengthens the inference of a causal association...

Ngày tải lên: 28/03/2014, 19:20

... protects cells from a variety of inducers of apoptosis, the effects of H2O2 have not yet been examined Rat1 cells and 5.61 cells were exposed to various concentrations of H2O2 and cell viability ... (Fig 3A) Surprisingly, the viability of 5.61 cells was significantly less than Rat1 cells at each concentration of H2O2 (Fig 3A) The viability of the entire panel of cell populations was examined ... Histogram of the percentage viability (MTT assay) of the indicated untreated (black columns) and 16 h of 750 lM H2O2 treated (grey columns) cell lines and populations The columns represent the mean of...

Ngày tải lên: 29/03/2014, 08:20

... an MOI of PFU/cell and cultured for 18 h, the profile of DNA content was different from that of mock-infected cells A broad peak was observed at the position of G0/G1 and the population of 100 ... Results are representative of independent experiments Effect of buffering [Ca2+]i on H2O2-mediated enhancement of viral release The effect of Ca2+ depletion on the release of HSV-1 was examined Eighteen ... ciated of1 2O2 on the amount of cell-free virus and cell-assoEffect virus Effect of H2O2 on the amount of cell-free virus and cell-associated virus FI cells were infected with HSV-1 at an MOI of...

Ngày tải lên: 20/06/2014, 01:20

... aspects 2.2 Kinetics of the decomposition of hydrogen peroxide In the case of the decomposition of hydrogen peroxide, the reaction is regarded as irreversible, since one of the products is gaseous ... in this study Most of the works in the literature focused on the use of hydrogen peroxide for the remediation of wastewater and the concentrations were very low (in the order of 10-3 mol/L) In ... RESULTS AND DISCUSSION 4.1 Estimation of kinetic parameters – assumption of first order kinetics (m=1) The extraction of the reaction rate constant, k, out of the heat data from experiments was...

Ngày tải lên: 22/07/2014, 03:20

... 3D software (BK3Di Type 6503) to reconstruct images Findings in operations were the gold standards Results: in 132 patients (109 males, 23 females, mean age 49.67), there were a variety of anal ... sonography was able to identify the inner opennings of all cases, particularly cases with two inner opennings Accordings to the classification of Parks et al, we had six submucosal fistulas, three ... muscle type (2 cases) Comparing the operation results the exact of endoanal ultrasonography with hydrogen peroxide in diagnosis of anal fistulas was 96.2% MỞ ĐẦU Phẫu thuật điều trị bệnh rò hậu...

Ngày tải lên: 01/08/2014, 16:20

... by a depletion of endogenous antioxidant moieties and an increased production of ROS Oxidative stress, in particular, is thought to be a cause of neuronal cell death in the brain of HIV-1 infected ... applying an excitation wavelength of 532 nm and an emission filter of 580BP30 nm Data were analyzed using ImageQuant™ TL software (GE Healthcare) The fluorescence intensity of medium containing only ... Importance of the nef gene for maintenance of high virus loads and for development of AIDS Cell 1991, 65:651-662 51 Simard MC, Chrobak P, Kay DG, Hanna Z, Jothy S, Jolicoeur P: Expression of simian...

Ngày tải lên: 11/08/2014, 21:21

... http://www.actavetscand.com/content/53/1/45 Page of 10 Figure Kaplan-Meier survival plot of days from start of the study until healing according to treatment in the majority of horses regardless of the day of sampling (day ... as no growth, sparse (n < 20), moderate (20 to 100), or profuse (> 100) on the basis of the number of colonies on the agar plates Growth of b-haemolytic streptococci, Staphylococcus aureus, Pseudomonas ... circulatory effects The shorter healing time of LHP® treated wounds could also be due to a beneficial effect of components of the cream base itself Of the components propylene glycol has been...

Ngày tải lên: 12/08/2014, 18:22

... Page of 11 Figure Effect of cAMP on allergen gene expression Relative expression levels on AMM + 1% glucose are shown; mM of dbtcAMP was added to the growth medium and the expression profiles of ... tested to give an indication of level of expression Error bars represent standard error of mean of biological replicates calculated using GraphPad PRISM 4.0 exception of Asp f 12 Asp f was repressed ... glucose showing detail of the low expression levels of Asp f 5, -6, -9, -10, -13 and -18 (same data as left panel) B, Expression levels of allergen genes in the presence of various oxidative stress...

Ngày tải lên: 13/08/2014, 13:22