... primers: Aba, 5Â-ATGGACGCTGAAT TCCGTCACGACTCTGGTTACGAAGTTCACCACCAG AAGCTGGTG-3Â;Abb, 5Â-GTTCACCACCAGAAGCT GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG GGTGCT-3Â;Abc, 5Â-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab- start, ... 5Â-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab- start, 5Â-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3Â;Abstop, 5Â-CCTGCCGAGCTCCTATTA CACAACGCCACCAACCATCAG-3Â. The PCR solution was prepared in the buffer supplied with ... than A b40 [32–34]. The morphol- ogy of aggregates formed after incubation times when the aggregation had reached a maximum [5 h for Ab(M1–40) and Ab (1 40) and 80 min for Ab(M1–42) and Ab (1 42)]...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... tsA58T Ag cDNA carry- ing the A4 38V mutation were PCR-amplified from COS-7 cDNAs using the following primers: LTA-1F, 5Â-CTC GAGATGGATAAAGTTTTAAACAGAG-3Â and LTA- 1R, 5Â-TGAAGGCAAATCTCTGGAC-3Â for ... 250 kDa 15 0 kDa 10 0 kDa 10 0 kDa 75 kDa 50 kDa 10 0 kDa 75 kDa Lyve -1 Prox -1 VEGFR-3 tsA58 T Ag / tublin Lyve -1 DaAPI DaAPI Lyve -1 Prox -1 CDa 31 DaAPI Magnetic A B C D E immunosorting ... Oreda B et al. (2003) The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. J Cell Biol 16 2, 11 11 11 22. 8...
Ngày tải lên: 18/02/2014, 17:20
Báo cáo khoa học: "A Nonparametric Method for Extraction of Candidate Phrasal Terms" docx
... Annual Meeting of the Association for Computational Linguistics, pages 18 8 -19 5. Ferreira da Silva, J. and G. Pereira Lopes (19 99). A local maxima method and a fair dispersion normalization for ... 605– 613 , Ann Arbor, June 2005. c 2005 Association for Computational Linguistics A Nonparametric Method for Extraction of Candidate Phrasal Terms Paul Deane Center for Assessment, Design and ... C- Value and NC-Value Method. International Journal on Digital Libraries 3(2) :11 5 -13 0. Gil, A. and G. Dias. (200 3a) . Efficient Mining of Textual Associations. International Conference on Natural...
Ngày tải lên: 08/03/2014, 04:22
Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
... physiologically feasible ranges: 1 2 k 0 ATPase k ATPase 2k 0 ATPase (small variation of the energetic load) 1 5 k 0 ATPase k ATPase 5k 0 ATPase (large variation of the energetic load) 1 50 k 0 ox ... 50.9 39 .1 17.2 19 .2 5.3 46 10 0 10 0 10 0 LL Hybrid 9.6 3.3 40.4 0 .1 1.4 61 100 10 0 10 0 Fully simplified 22.3 13 .7 41. 0 0.4 5.9 84 10 0 10 0 10 0 MA Hybrid 14 .2 3.7 16 .2 0 .1 3.4 10 0 91 100 10 0 Fully ... increased value of k ATPase as compared to the value k 0 ATPase ẳ 1: 6h 1 . At values of k ATPase exceeding seven-fold of its normal value, no stationary states can be found; that is, k max ATPase ẳ...
Ngày tải lên: 23/03/2014, 06:20
USAID Supported a Wide Range of Child and Maternal Health Activities, but Lacked Detailed Spending Data and a Proven Method for Sharing Best Practices pptx
... 9,207 Honduras 3 ,14 2 3 ,14 3 3,377 India 12 ,600 14 ,222 12 ,852 Indonesia 11 ,400 13 ,800 14 ,15 7 Jamaica 544 539 497 Kenya 1, 000 1, 000 989 Liberia 1, 200 1, 200 1, 582 Madagascar 2,825 3,475 3,287 Malawi 2,200 ... Percent Africa $78.6 24.0% $88.3 25.4% $16 6.9 24.7% Asia and the Near East 79.6 24.2 80.5 23.2 16 0.0 23.7 Latin America and the Caribbean 39.0 11 .9 39.3 11 .3 78.4 11 .6 International Partnerships 64.2 19 .6 ... 20.8 13 6.5 20.2 Bureau for Global Health 66.0 20 .1 67 .1 19.3 13 3 .1 19.7 Other 0.6 0.2 n /a n /a 0.6 0 .1 Total $328.0 - $347.5 - $675.6 - Source: GAO analysis of USAID data. Page 47 GAO-07-486...
Ngày tải lên: 28/03/2014, 09:20
Báo cáo khoa học: "A Bayesian Method for Robust Estimation of Distributional Similarities" pot
... 48th Annual Meeting of the Association for Computational Linguistics, pages 247–256, Uppsala, Sweden, 11 -16 July 2 010 . c 2 010 Association for Computational Linguistics A Bayesian Method for Robust ... Shaul Marcus, and Shaul Markovitch. 19 95. Contextual word similarity and estimation from sparse data. Computer, Speech and Language, 9 :12 3 15 2. Ido Dagan, Lillian Lee, and Fernando Pereira. 19 97. Similarity-based ... Estimation of Distributional Similarities Jun’ichi Kazama Stijn De Saeger Kow Kuroda Masaki Murata † Kentaro Torisawa Language Infrastructure Group, MASTAR Project National Institute of Information...
Ngày tải lên: 30/03/2014, 21:20
Báo cáo khoa học: "A Unification Method for Disjunctive Feature Descriptions" pot
... a formula contain informa- tion that is more definite than the information contained in disjunctions. Thus a formula can be regarded as having a definite part, containing only unconditional ... Berkeley, California, February 17 -19 , 19 79. [7] Kay, M. Parsing in Functional Unification Grammar. In D. Dowty, L. Karttunen, and A. Zwicky, editors, Natu- ral Language Parsing. Cambridge ... University Press, Cam- bridge, England, 19 85. [8] Perelra, F. C. N. and D. H. D. Warren. Definite clause grammars for language analysis - a survey of the formal- ism and a comparison with augmented...
Ngày tải lên: 31/03/2014, 17:20
a novel method for the synthesis of titania nanotubes using
... the annealed titania nanotubes are crystalline (mostly anatase) and show varied activity de- pending on the material preparation and annealing atmosphere (Fig. 12 ). Titania nanotubes prepared ... mag- netically stirred, anodized titanium samples are designated in the main text as UAT and SAT, respectively. 2.3. Annealing of the materials The anodized titania nanotubular arrays were annealed ... well-ordered titania nan- otubes. The anodization approach builds self-organized titania nanotubular arrays of controllable tube diameter, good unifor- mity, and conformability over large areas. Sonochemistry...
Ngày tải lên: 05/05/2014, 15:26
a novel method for the synthesis of cao nanoparticle
... The peaks at 16 32 and 14 93 cm -1 are assigned to CO2 absorbed on the surface of nanoparticles. The peaks at 13 50 and 898 cm -1 are assigned to C-H and C-C bonding vibrations of organic impure ... 13 00 (2 012 ). [34] B. K. Olga, L. Isabelle, V. Alexander, Chem. Mater., 9, 24 (19 97). [35] R. Arup, B. Jayanta, Int. J. Nanosci., 10 , 413 (2 011 ). [36] K. Masato, K. Takekazu, T. Masahiko, S. ... 1) AUC/2-CEPS(2)AUC/Toluene (1) Ratio Sam ple 10 0.000.5744260693453933BlankA 84.69 0. 4864 16 28 91 3 34852 1: 10 B 61. 88 0. 3554 12 17 91 3 426 51 1:20 C 33.53 0 .19 25 83649 43 4328 1: 30 D 00.00 0 0 42 8 017 1: 40 E M. Sadeghi...
Ngày tải lên: 06/05/2014, 08:55
Báo cáo hóa học: "One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas" docx
... sequences for amplification of CEA were: 5’ - AGACAATCACAGTCTCTGCGGA-3’ (forward) and 5’ - ATCCTTGTC CTCCACGGGTT-3’ (reverse). The cut-off was set at cycle time = 29.6 for CK19, 28.5 for CEA, and 30.0 ... CK19 IHC on 5 levels for each of 2 LN slices). RNA quality was assured by OSNA performed for beta-actin. 13 9 samples gaveanegativeresultand37samplesgaveapositive result with both methods (table ... colorectal cancer to predict micrometastases. Arch Surg 2002, 13 7 :13 77-83. 20. Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F, Kato Y, Tsuda H, Ueda S, Sato K, Tamaki Y,...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... times a week. The database will be made available free of charge to inter- ested parties. Table 7: Amplification efficiency for patients' amplicons Genotype 1a Amplicon A1 A2 A3 A4 x A4 y Amplification ... A4 y Amplification efficiency 95 a 98 93 10 0 95 Average efficiency 96.2 Genotype 1b Amplicon A1 x A1 y A2 A3 A4 x A4 y Amplification efficiency 10 0 10 0 93 93 10 0 10 0 Average efficiency 97.7 a Amplification ... until at least three pairs of optimized prim- ers were available for each amplicon. Table 1 (see additional file 1: HCVMethodPaperTable1.xls) and Table 2 (see additional file 2: HCVMethodPaperTable2.xls)...
Ngày tải lên: 19/06/2014, 08:20
Báo cáo hóa học: " A novel method for neck coordination exercise – a pilot study on persons with chronic non-specific neck pain" docx
... ranged from 11 to 20. Therefore, for block 1 11 n = 14 and for block 12 –20 n = 13 , 11 , 11 , 10 , 9, 8, 6, 3 and 1 respectively. For each box plot, the whiskers represent maximum and minimum values, ... like to thank Nisse Larson for excellent engineering assistance, Maria Frykman for valuable assistance during data collection and Margaretha Marklund for graphical work. References 1. Guez M, ... 95 :14 10 -14 18. 24. Woltring HJ: A fortran package for generalized, cross-valida- tory spline smoothing and differentiation. Advances in Engineer- ing Software and Workstations 19 86, 8 :10 4 -11 3. 25. van...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học: "A binary method for simple and accurate two-dimensional cursor control from EEG with minimal subject training" potx
... Piccione's system had an average accuracy of 76.2% and a bit rate of 7.59 bits/min with healthy trained subjects [15 ]. Based on these results, our method appears to have a higher accu- racy at a ... an optimum channel/bin, so we used Bhattacharyya distance plots for real movementFigure 2 Bhattacharyya distance plots for real movement. Higher values indicate greater class separability. (a) ... effective channels and frequency bands for control. We calculated each Bhattacharyya dis- tance according to (1) , where M i and Σ i are the mean vec- tor and covariance matrix of class i ( = 1, 2),...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... times a week. The database will be made available free of charge to inter- ested parties. Table 7: Amplification efficiency for patients' amplicons Genotype 1a Amplicon A1 A2 A3 A4 x A4 y Amplification ... A4 y Amplification efficiency 95 a 98 93 10 0 95 Average efficiency 96.2 Genotype 1b Amplicon A1 x A1 y A2 A3 A4 x A4 y Amplification efficiency 10 0 10 0 93 93 10 0 10 0 Average efficiency 97.7 a Amplification ... 11 ):23 91- 2399. 11 . Martell M, Esteban JI, Quer J, Genesca J, Weiner A, Esteban R, Guar- dia J, Gomez J: Hepatitis C virus (HCV) circulates as a popula- Additional File 1 Primers for amplification...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:" A rapid method for the generation of uniform acellular bone explants: a technical note" pot
... data analysis and the preparation of the manuscript. MJS supervised the study plan- ning, data analysis and preparation of the manuscript. All authors read and approved the final manuscript. Acknowledgements The ... Volker Braunstein 3 , Pamela I Furlong 1 , Angharad E Simpson 1 , R Geoff Richards 1, 2 and Martin J Stoddart* 1 Abstract Background: Bone graft studies lack standardized controls. We aim to present ... Correspondence: martin.stoddart@aofoundation.org 1 AO Research Institute, Clavadelerstrasse 8, 7270 Davos, Switzerland Full list of author information is available at the end of the article Jähn et al....
Ngày tải lên: 20/06/2014, 04:20
Báo cáo toán học: " A novel method for crystalline silicon solar cells with low contact resistance and antireflection coating by an oxidized Mg layer" ppt
Ngày tải lên: 20/06/2014, 21:20