beadchip™ molecular analysis of proteins and nucleic acids

... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of ... higher than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides and proteins charged) indicates that the Tyr residues at positions 31 and 40 are both 80 mass units ... breakthrough in the field of proteomics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer The scan times required for direct analysis of proteins using ETD...

Ngày tải lên: 18/02/2014, 16:20

... analyses of single protein and simple protein complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) ... 3D proteomics: Analysis of proteins and protein complexes by chemical cross-linking and mass spectrometry Zhuo A Chen Thesis for the Degree of Doctor of Philosophy The University of Edinburgh August ... spectrometric analysis of cross-linked proteins, which is the ‘top-down’ approach In this technique intact cross-linked proteins are analyzed CHAPTER The accurate measurement of the mass of proteins...

Ngày tải lên: 30/09/2015, 05:51

... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... Birken S, Axel R and Cantor CR Molecular cloning and nucleotide sequence of the streptavidin gene Nucleic Acids Res 1986, 14:1871-1882 Blake KR, Murakami A and Miller PS Inhibition of rabbit globin ... specific sequences) for nucleic acid-binding proteins and should also encode corresponding nucleic acid-binding proteins The use of known protein transcription factors and their target DNA sequences...

Ngày tải lên: 11/08/2014, 00:21

... of cardosin A) Two proteins with apparent molecular masses  90 kDa and 67 kDa were isolated on elution with an RGDS peptide (Fig 1A) The 90-kDa protein has a molecular mass similar to that of ... terms of charge and is positioned at the tip of a loop and is therefore reminiscent of RGD sequences present in integrin-binding molecules because of its exposed location On the basis of these structural ... superimposition of the 3D structures of kistrin [32] and cardosin A (Fig 8) The importance of both motifs and in particular their basic residues was further emphasized by the complete lack of interaction...

Ngày tải lên: 30/03/2014, 11:20

Ngày tải lên: 07/08/2014, 17:22

... (Figs and 2) Analysis of hprt mutations in T-cells from spleens of control and test materials-exposed B6C3F1 mice Analysis of the spontaneous hprt mutant clones yielding The toxicologic actions of ... phosphoribosyltransferase (hprt) locus of lymphocytes isolated from spleens of mice following exposure to ozone, NNK, and DBP, and combined treatments of NNK and DBP on ozone for 32- and 52-wk The procedures ... Douglas GR, Soussi T Databases and software for the analysis of mutations in the human p53 gene, the human hprt gene and the lacZ gene in transgenic rodents Nucleic Acids Res 1996, 24, 119-120 Cariello...

Ngày tải lên: 07/08/2014, 18:20

... detected in 4% of the cases with specific RFLP profile of A1 pattern (301 pb, 121 pb, 57 pb with AvaII and 318 pb, 109 pb and 52 pb with DpnII) In 7% of the cases, a restriction profile Hannachi ... NH and OB conceived of the study, participated in its design, and in drafting the manuscript HT and JB participated in the study design and coordination and in discussing manuscript NH, NBF and ... the risk of evolution to cirrhosis and HCC [11] This type of mutants seems to be frequent in our population in view of the fact that 59% of the patients are characterized by the absence of HBeAg...

Ngày tải lên: 12/08/2014, 02:20

... destination (37% of the proteins; decreased volume in 66% of the proteins of this group was observed) Most of the proteins associated with dormancy breaking in the presence of ABA (Fig 3B) were ... pattern of 71 proteins, with a shift away from accumulation of a major group of proteins, involved mainly in energy and protein metabolism [5] The same negative effect of ABA was observed on proteins ... deficit and storage [24] Proteomic analysis of the seeds of another woody plant, Prunus campanulata, provide evidence of the involvement of prunin and dehydrin in the response to warm and cold...

Ngày tải lên: 12/08/2014, 03:20

... PROTEIN AND MOLECULAR ANALYSIS OF YEAST FACTOR PMP3P DURING AGROBACTERIUM-MEDIATED TRANSFORMATION LI XIAOYANG (B Sc Science.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... assay of VirE2 in yeast cells 68 Figure 3.22 GFP11 does not perturb the function of VirE2 in AMT of plants 71 Figure 3.23 Study of putative nuclear localization signals of VirE2 in AMT of ... components, inner membrane associated proteins, VirD4, VirB4, and VirB11, form the base of the secretion structure All of these proteins contain NTP-binding domain and are supposed to provide energy...

Ngày tải lên: 09/09/2015, 11:19

... 2.2 The molecular function of OSBP…………………………………………… 22 21 2.3 The molecular function of ORPs……………………………………………….….24 23 2.4 The molecular function of OSHs 2.4.1 Secondary structures of Osh proteins ………………………………….…… ... precursors of steroid hormones, bile acids and lipoproteins (Liscum and Underwood, 1995) Since sterols are so important for a variety of cellular activities, their biosynthesis, metabolism and intracellular ... synthesis of acetoacetyl-CoA from two molecules of acetylCoA, which is derived from several sources including: the β-oxidation of fatty acids, the oxidation of ketogenic amino acids such as leucine and...

Ngày tải lên: 16/09/2015, 08:31

... chuyển tiếp dị thể n-Si/p-CIS 14 CHƯƠNG CHƯƠNG TRÌNH MÔ PHỎNG MỘTCHIỀU AMPS – 1D (Analysis of Microelectronic and Photonic Structures) Chương trình mô chiều AMPS – 1D chương trình đa để khảo sát...

Ngày tải lên: 18/03/2013, 13:40

... CHEMICAL ANALYSIS OF WATER AND WASTEWATER 24.1 GENERAL ASPECTS OF ENVIRONMENTAL CHEMICAL ANALYSIS Scientists’ understanding of the environment can only be as good as their knowledge of ... of the identities and quantities of pollutants and other chemical species in water, air, soil, and biological systems Therefore, proven, state -of- the-art techniques of chemical analysis, properly ... in the evolution of analytical chemistry, characterized by the development of new and improved analysis techniques that enable detection of much lower levels of chemical species and a vastly increased...

Ngày tải lên: 23/07/2013, 20:57

... Definition of the energy analysis parameters The energy analysis and simulation of buildings requires the definition of a number of parameters, related to construction characteristics (geometry and ... Dioxide (CO2) footprint Table Energy and environmental indices per building type and year of construction Building Type Classrooms Offices Offices Offices Offices Offices Theatres Laboratories Construction ... Member of the Technical Chamber of Greece, and Member of the Union of Mechanical and Electrical Engineers of Northern Greece E-mail address: vvoulgar@gmail.com Nikolas Kyriakis is the Director of...

Ngày tải lên: 05/09/2013, 16:10

... Idealism: Negotiation and Administration in Hegel’s Account of the Structure and Content of Conceptual Norms,” European Journal of Philosophy, () (August ), –, and Tales of the Mighty Dead, ... of the world-process of things coming-to-be and passing-away taken as a whole This world-process of coming-to-be and passing-away is thus all that there is, and it is within this conception of ... shared some of its inspiration The most extensive comparison and critique of Hegel’s Logic from the standpoint of Fregean and post-Fregean formal logic is to be found in Pirmin Stekeler-Weithofer,...

Ngày tải lên: 01/11/2013, 08:20

... constituent parts interrelate and interact together in a kind of discourse 21 - Comparative and contrastive methods: The results of analytic and synthetic data analysis of opening and closing speeches ... description of samples, data analysis and research procedures Chapter 4: Findings and Discussion It is concerned with the discourse features of American English and Vietnamese opening and closing ... discourse analysis, a distinction is often made between spoken and written discourse In terms of lexical choice and density, written texts seem to use more complex and diversified words and abstract...

Ngày tải lên: 26/11/2013, 12:41

... discover similarities and differences of these features between the two languages and help Vietnamese learners of English understand and grasp the language characteristics of English advertisements ... beauty of language, strategies and techniques through the use of layout, syntax, lexis and devices that are expected to be beneficial For the above reasons, “A Discourse Analysis of English and ... features of EAPs and VAPs in terms of their layout, lexical features, syntactic features and cohesive devices? What are the similarities and differences between EAPs and VAPs in terms of their...

Ngày tải lên: 26/11/2013, 13:26

... recognition of the field And the findings of semantic aspects often borrow the WDBs for not only conveying the denotative as well as analysis of pragmatic aspects of these kinds of words will meaning ... teaching and learning of English 3 1.4 RESEARCH QUESTIONS: (1) What are the similarities and differences of semantic features of the WDBs in English and Vietnamese? (2) What are the similarities and ... important and significant characteristics of the WDBs As a result, the topic “A Contrastive animals that nature has provided to feed both our body and spirit As Analysis of the Semantic and Pragmatic...

Ngày tải lên: 26/11/2013, 13:30

... similarities and differences between WIRHs in So far there has been no contrastive analysis of grammatical English and Vietnamese in terms of grammatical features? and semantic features of words and idioms ... features of WIRHs in English and - to describe semantic features of WIRHs English and Vietnamese - to provide a brief account of grammatical-semantic similarities and Lê Lan Phương has a study of verbs ... the grammatical and semantic features of some verbs learners, users or translators of English often meet a lot of difficulties and idioms related to hearing (WIRHs) in English and Vietnamese in...

Ngày tải lên: 26/11/2013, 13:31

... terms of Noun, Verb, Adjective and Adverb The third step is to discuss the result of analysis and giving explanations for the similarities and differences in terms of grammatical, semantic and ... features of the WsDMC in English and Vietnamese - Scores of similarities and differences between the sociocultural features of WsDMC in English and Vietnamese From which we draw conclusions and generalize ... similarities and differences on specific sociocultural features of the WsDMC in English and Vietnamese? 1.5 SCOPE OF RESEARCH Due to time, length limit of the thesis and the ability of our own,...

Ngày tải lên: 26/11/2013, 13:31

... about Theme and Rheme, although "The Analysis of Theme and Rheme in "Little House on the Prairie" is shown as the theme of the study Method of the Study - A great number of documents and materials ... out of the context Moreover, the common traditional practice of language teaching and learning has been on the analysis of single sentences, normally at the levels of phonology, vocabulary, and ... "Little House on the Prairie" as the source of data to the study 2.4 Principles of Analysis In our study, a linguistic description and analysis of Theme and Rheme is carried out Since the study...

Ngày tải lên: 22/12/2013, 12:59