... Chan and the staff of Sunway Medical Center, Malaysia for provision of samples, permission and technical assistance in the use/help of their diagnostic facilities. Conflict of interest The ... Discipline of Medicine, Blackburn Building D06, University of Sydney, NSW 2006, Australia 4. Institute of Molecular and Cell Biology, 61 Biopolis Drive (Proteos), 138673, Singapore 5. Department of ... levels of both HBeAg and anti-HBe were performed based on the mean rate of the Index Calibrator provided and calculated as the cutoff rate, CO. The calculation was based on the ratio of the...
... particular,phosphorylation of dGuo was reduced markedly.Structure of Dm-dNK-E52D in complex with dTTPThe structure of one of the mutants, E52D, was solvedusing X-ray crystallography at a resolution of 2.5 A˚incomplex ... levels of phosphorylation expressed in rela-tion to percentage dThd phosphorylation of the wild-type. Relativephosphorylation expressed as a relation of the percentage of dThdphosphorylation of ... quantitation of microgram quantities of protein util-izing the principle of protein–dye binding. Anal Biochem72, 248–254.22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B &Loffler M (1996)...
... upper bound of 80% PEP from P5P (Fig. 6).The PfkA mutant is deficient in the allosterically regula-ted, major isoform of phosphofructokinase that constitutesabout 90% of the total phosphofructokinase ... andexpression of the fumarase gene of Escherichia coli K-12. J. Bac-teriol. 153, 588–596.31. Model, P. & Rittenberg, D. (1967) Measurement of the activity of the hexose monophosphate pathway of glucose ... Massdistributions of metabolite fragments (MDVM)arecalculatedfromMDVAAby using Eqn (4). (B) MDVM of different metabolites areused to calculate split ratios of diverging pathways and the MDV of CO2according...
... Crystal-lographic analysis of DAOCS complexed with iron(II),succinate and CO2demonstrated that the 4-carboxylate of succinate may be bound by the side-chain of R160 or R162 inthe absence of any substrate ... cosubstratebinding. In the case of the R258K mutant this may beallowed because of disordering of the lysine side-chain, aswas previously observed for the R258Q mutant (Fig. 1) [17].InthecaseoftheR258HmutantitismorelikelythattheFig. ... The effect of mutations appears to be similar regardless of whether the prime substrate was penicillin G or ampicillin.Utilization of 2-oxoglutarate by the R258 mutantsThe level of 2-OG conversion...
... (22.7) 8.8 (24.5)Number of protein atoms 2083 2081 2080 2079Number of carbohydrate atoms 57 57 71 57Number of sulfate ions 1 1 3 1Number of glycerol molecules – – 5 –Number of water molecules ... poly (N-acetylglucosamine), is one of the most abundant polymers in nature. It is a majorcomponent of the cell wall of yeast and other fungi, and theexoskeleton of arthropods. Although chitin is ... catalytic role of Asp125 has also beendeduced from crystal structures. The side chain O1 atom of Asp125 is at hydrogen bonding distance from the amidenitrogen of the N-acetyl group of the )1 sugar...
... thereduction of methanofuran and CO2to formyl-methanofuran. The overall reduction of CO2and meth-anofuran by H2is endergonic and is driven in vivoby reverse electron transport [10]. Reduction of ... subunits of all [NiFe] hydrogenases. Chem-ical analysis has revealed the presence of Ni, nonhemeFe and acid-labile S in a ratio of 1 : 12.5 : 12 [6], cor-roborating the presence of three [4Fe-4S] ... clusters.Characterization of the iron–sulfur clusters of theenzyme by electron paramagnetic resonance spectros-copy (EPR) showed that two of these clusters could befully reduced under 105Pa of H2at pH...
... (Fig. 3) with a turnover number (kcat )of8 .7s)1,Km,gal of 970 lMand Km,ATP of 34 lM. These values are of the same order of magnitude as previously reported for theyeast [23], rat [18,19] ... cloned into the NcoIand EcoRI sites of pET21d (Novagen). The DNA sequence of the entire GALK1 coding sequence was determined(University of Manchester, Faculty of Medicine DNASequencing Facility).The ... determining step of GALK1Although the enzymes of the GHMP family share sequenceand structural similarity, there are differences in themechanism of catalysis. The structure of mevalonate kinaseshows...
... incident of the battle and defeatwould have been mentioned and passed without comment.THE COVERT RETREAT OF THE BRITISH.The battles of the eighth were decisive of the campaign, and of the War of ... the War of 1812 as the most important of the many that were waged. These three were, first, the battle of Tippecanoe, regarded as the opening scene of the bloody drama; second, the battleof the ... profound impression on the mind of General Jackson. Theenemy had already opened the battleof the twenty-eighth of December, with the forward movement of hiscolumns and under the heavy fire of...
... xiTHE BATTLEOF GETTYSBURG. Frank Aretas Haskell 1ILLUSTRATIONSPAGEPORTRAIT OF AUTHOR, while Colonel of Thirty-sixth Wisconsin Infantry FrontispieceMAP OFBATTLEOF GETTYSBURG, JULY 2 58MAP OF ... assault of the enemy. But fresh bodies of theRebels continued to advance out of the woods to the front of the position of the Third Corps, and to swell thenumbers of the assailants of this ... from the scene of operations in front, we got many rumors, and much disjointed information of battle, of lakes of blood, of rout and panic and undescribable disaster, from all of which the narrators...
... there."In his official report of the fight, General Gibbon says: "I desire to speak in the most commendatory terms of the conduct of both officers and men (with the exception of the two cowards ... Thirteen of his seventeen officers were veterans of the war of the Rebellion, as were nearly all the citizen volunteers. The other four officers, and nearly all the enlistedmen had seen years of hard ... nobly.CHAPTER III. 24The Battleof the Big Hole, by G. O. ShieldsThe Project Gutenberg EBook of The Battleof the Big Hole, by G. O. Shields This eBook is for the use of anyone anywhere at no...
... peptides show two sets of resonances for anumber of C-terminal residues. Because of the lack of Metwe can exclude oxidation to methionine sulfoxide as thecause of the second set of resonances for ... height of thecross-peaks was used for a conservative estimation of themaximum distances and classification of cross-peaks asweak, medium and strong. For the calibration of theintensities of the ... showsMOLMOLstereographic projections [19] of the heavy atoms of rSP-C (FFI). The structure of rSP-C (FFI) is a well-defined a-helix ranging from Phe5 toLeu34. Note that the distribution of the / and w anglesindicates...
... was eluted as a mixture of a dimer and a tetramer.For further investigation of the role of size, conformationand polarity of amino acid 106 for the function andstructure of human TK1, we created ... Furthermore, aprediction of the secondary structure of TK1 [30–32] placesVal106 in the middle of an a-helix which aligns inCLUSTALW[33] with one of the interface helices (a-helix 4) of HSV1-TK. This ... 1). Although the –ATP form of V106K does notgain the Vmaxvalue of its +ATP form, both the +ATPand –ATP forms have low K0.5values of 0.7 and 1.2 lMandn values of 1.4, similar to the other...
... maintenance of theenterohepatic circulation of bile acids and therefore alsoparticipate in the homoeostatis of cholesterol. Members of SBATs are located either in apical membranes of ileumenterocytes, ... domains model), is involved in taurocholatetransport of the human isoform [21]. We therefore lookedfor the role of each of the eight cysteines of the rat Ntcp fortaurocholate transport.Finally, ... Disturbances of thehepatocellular part of the enterohepatic circulation of bileÓ FEBS 2003 Site-directed mutagenesis of Ntcp (Eur. J. Biochem. 270) 1123200 mM. The buffers of zero, 25, 50,...
... friendship of Arnold of Rugby, of Matthew Arnold the son, of Arthur Hugh Clough, and of ThomasCarlyle. He returned from his honours in England to find himself the centre of the intellectual movement of New ... heresy."In 1811, as one of the results of the purchase of Louisiana by Jefferson, a bill had been offered providing forthe reception of the State of Orleans into the Union. The people of New Orleans ... the kidnapping of African slaves, and the sale of these men to thesugar and cotton planters of the West Indies and of America. Even the stories of the gold and diamond fields of South Africa...
... reader is presentedthe classic battle between the deceitful forces of evil and the innocence of good. It are these forces of evil that ultimately lead to thebreakdown of Othello, a noble venetian ... demise of others. It is this that is Iago's motivation. The ultimatedefeat of good by the wrath of evil. Not only is it in his ownnature of evil that he suceeds but also in the weaknesses of ... now that he convinces himto consider getting rid of Desdemona and even suggestsmethods of killing her. Iago, so proud of his accomplishments of underhandedness : Work on. My med'cine works!...