... the wedge hypothesis of Tytler et al [48], it may be that insertion of the inverted wedge shape formed by the AP1 a-helix into membranes of E coli leads to the formation of nonbilayer structures ... periodicity of 100 The resultant of this summation, the hydrophobic moment, provides a measure of a-helix amphiphilicity The analysis of the present study used a moving window of 11 residues, and for ... were formed by spreading on to a buffer subphase, chloroform solutions of Ole2PtdGro, Ole2PtdEtn and cardiolipin in molar proportions of : 13.67 : [53] The solvent was allowed to evaporate off...
Ngày tải lên: 07/03/2014, 12:20
... 5¢-end of loop In both loops, 6-mer oligomers hybridize to the 5¢-end of the loops and propagate to nucleotides involved in the formation of the helix The strong preference for the 5¢-side of the ... probably coordinated to N7 atom of the imidazole ring of guanines Yet the possibility that deletion of the N7 group of purines may result in an alternative structure of the aptamer cannot be excluded ... Interestingly, similar binding properties of Co2+ to the N7 atom of guanine residues were previously observed in the crystal structure of the orthorombic form of yeast tRNAPhe [23] In the predicted...
Ngày tải lên: 16/03/2014, 06:20
Structural basis for recruitment of traffic proteins by small GTPases
... STRUCTURAL BASIS FOR RECRUITMENT OF TRAFFIC PROTEINS BY SMALL GTPASES WU MOUSHENG (B.Sc., M.Sc) Xiamen University A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... structures of complexes of Ras with the CDC25 domain of SOS and of Arf1 with the Sec7 domain have been reported (Boriack-Sjodin et al., 1998; Goldberg, 1998) These two structures form the basis of our ... Dynamin around the neck of the bud help it pinch off to form a vesicle The coat of clathrin-coated vesicles is removed rapidly after the vesicle forms c Complementary sets of v-SNAREs and t-SNAREs...
Ngày tải lên: 16/09/2015, 08:31
Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... the three Drosophila isoforms and two Leptinotarsa isoforms Such quantitative differences may prove important for in vivo functions In Manduca, the presence of a B-isoform increases transcriptional ... quantitative level of transcriptional activity, with both its own EcR isoforms, and with the two L decemlineata EcR isoforms Discussion 10 B 40 3.0 A controlled assessment and comparison of the Leptinotarsa ... quantitative level of transcription for a given isoform, it had no effect on relative ligand responsiveness Importantly, the relative induction by individual agonists was species-specific for all of the tested...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis doc
... intermediate Rate constant for decay of intermediate Lag phase preceding MgDME product formation Magnitude of burst in product formation Rate constant for MgDME product formationa a ChlM ChlM + ... kinetic parameters for Synechocystis ChlM, and the effects of the magnesium chelatase ChlH subunit (rate constants refer to Scheme 1) Parameter MgD K app Rate constant for formation of catalytic intermediate ... were performed in the presence (s) and absence (d) of lM ChlH K app MgD ¼ 17.3 ± 3.3 lM in the presence of ChlH K app MgD ¼ 24.3 ± 7.5 lM in the absence of ChlH in the absence and presence of 0.75...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf
... growth of peaks corresponding to the anomeric resonances of the 4-keto (compound B) and gem-diol (compound C) forms of GDP-6-deoxy-d-lyxo-hexos-4-ulose (Fig 4B) On the basis of integration of the ... performed at 22 kV Peak integration was performed using 32 karat software (Beckman) Determination of kinetic parameters for GMD Reactions were performed in triplicate, and contained 0.25 lg of ... sugar nucleotides, and was our method of choice for initial in vitro characterization of these enzymes Analysis of the products that were formed after incubation of His6-GMD with GDP-d-Man indicated...
Ngày tải lên: 16/03/2014, 01:20
Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx
... control of substrate binding (A) Comparison of the lid regions of VioC (blue) and AsnO (green) The side chain of Ser224 forms a hydrogen bond with Gln137 that coordinates the a-amino ˚ group of hArg ... oxidative decomposition of a-KG forms succinate and CO2 and leads to the formation of an Fe(IV)-oxo species that abstracts a hydrogen radical from the unactivated methylene group of the substrate [24,25] ... model Crystals of VioC were assigned to space group C2 Each asymmetric unit contains one VioC molecule, which was defined for Val21–Gly356 The structure of VioC consists of a core of nine b-strands...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx
... – as a precursor of ADP for the first step and as an effector that causes conformational change of factors into low-affinity forms of enzymes to be reactivated The DhaF and DhaG of Citrobacter freundii ... molecular basis of the specificities of reactivating factors in the reactivation of inactivated holoenzymes and the activation of inactive enzyme–cobalamin complexes, the possibility of cross-formation ... MgCl2, in a total volume of 25 lL The amount of propionaldehyde formed was determined as described in the text The extents of activation of diol dehydratase by DDR and of glycerol dehydratase...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo Y học: Structural basis for the inhibitory efficacy of efavirenz (DMP-266), MSC194 and PNU142721 towards the HIV-1 RT K103N mutant doc
... This property is of general importance for the efficacy of the inhibitor Substitution of the hydrogen-donating amide group for a methylene group completely abolishes the activity of a MSC194-related ... In support of an alternative resistance mechanism is the observation of a hydrogen-bond network as a direct result of the mutation The network could stabilize the closed conformation of the NNIBP ... the hydrogen bond formed between the NNRTIs and the backbone carbonyl of K101 Omit electron density maps of the RT/NNRTI complexes clearly show the orientation and conformation of the inhibitors...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt
... position of mannose is drawn in light green, as is the corresponding orientation of the side chain of Glu221 The second conformation of the side chain of Glu221, which also adopts two conformations ... site of subunit A, but adopts an a ⁄ b mixture in the binding site of subunit B Accommodation of the b-configuration in the binding site of PAL requires a change in the rotamer conformation of Glu221 ... likely real and not a consequence of the correlation of fitting parameters often observed for weak binding events Evidence for this stems from the reproducibility of this result as well as from the...
Ngày tải lên: 23/03/2014, 11:20
Báo cáo khoa học: "A BASIS FOR A FORMALIZATION OF LINGUISTIC STYLE" pot
... most cohesive relation, all of the degrees assigned for the different types of ellipsis will be ranked in the top half of the scale of cohesiveness The first type of ellipsis which Halliday and ... unfulfilled expectations of (4) Thus, using the cohesive relations of Halliday and Hasan, it is possible, as I have shown, to provide a formal basis for the connective primitive elements of the syntactic ... point out, m a n y forms of verbal ellipsis are very diiticnlt to detect, due to the complexity of the English verbal group Because of this, I will deal only with two simple cases of verbal ellipsis:...
Ngày tải lên: 23/03/2014, 20:20
Báo cáo khoa học: Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx
... were examined for growth, +, in the presence of different concentrations of thymidine in the medium In the last column, LD100 values are given (in lM) for the growth of KY895 transformed with various ... responsible for the observed changes in the double mutant MuD We also present the crystal structures of Fig Location of mutated residues A monomer of Dm-dNK showing the location of Asn45 and ... kcat ⁄ K0.5 of the mutants compared to kcat ⁄ K0.5 of Dm-dNK (-fold) dTTP concentrations, the kinetics was clearly competitive with a Kic value of lm For comparison, the Kic value of dTTP with...
Ngày tải lên: 30/03/2014, 20:20
Báo cáo khoa học: Molecular basis for substrate recognition and drug ˚ resistance from 1.1 to 1.6 A resolution crystal structures of HIV-1 protease mutants with substrate analogs pptx
... from PR–p2-NC was observed for the Fig Residues with alternate conformations The number of occurrences of alternate conformations for each residue in the A and B subunits of the eight crystal structures ... 6A) mainly by movements of the side chains of P1 Nle and P3¢ Arg ˚ and partially by the small (0.3 A) shift of the CA atom of Ala82 ⁄ 82¢ P1¢-Nle showed three conformations for the side chain and ... contacts with the CB atom of Ala82 in PRV82A than observed for Val in the ˚ PR The CE atom of P3¢ Arg moved 1.2 A and formed closer interactions with the CB atom of Ala82 All of these observed structural...
Ngày tải lên: 30/03/2014, 20:20
Báo cáo khoa học: A structural basis for the pH-dependence of cofilin F-actin interactions potx
... roughly a third of the surface of cofilin No evidence for pH dependent conformational changes in the structure of cofilin We could find no evidence for substantial pH-dependent conformational changes ... this was the case for both ADP and ATP–actin (not Fig Effect of pH on the interaction of actin with cofilin (A) Effect of pH on the interaction of RITC-actin with cofilin Binding of RITC-labeled actin ... the affinity of binding of cofilin to unmodified ADP-G-actin and ATP-G-actin as a function of pH by direct means in order to confirm that the lack of pH sensitivity was not an artifact of labelled...
Ngày tải lên: 31/03/2014, 09:20
SUPERVISORY, INSTRUCTIONAL SKILLS AND PROFESSIONAL PERFORMANCE OF TEACHERS IN VOCATIONAL COLLEGES IN NGHE AN PROVINCE: BASIS FOR DEVELOPING AN ENHANCEMENT PROGRAM
... Socialist Republic of Vietnam (NAME OF PROFESSOR) (NAME OF PROFESSOR) Member Member (NAME OF PROFESSOR) Chairman Accepted in partial fulfillment of the requirements for the degree Doctor of Philosophy ... Vocational College of Trade and Tourism 82 11 Table 11 The Professional Performance of Teachers of Viet Duc Vocational College 84 12 Table 12 The Professional Performance of Teachers of Viet Han Vocational ... Skills of Teachers as to Classroom Management Skills 77 Table The Level of the Instructional Skills of Teachers as to Assessment Skills 79 10 Table 10 The Professional Performance of Teachers of...
Ngày tải lên: 13/05/2014, 14:46
ENHANCING THE COMMUNICATION SKILLS OF EDUCATIONAL MANAGERS IN VINH PHUC PROVINCE: BASIS FOR A TRAINING PROGRAM
... Percentage Distribution of Respondents Profile in terms of Length of Work Experience 61 Frequency and Percentage Distribution of Respondents Profile in terms of Number of Years in Management ... work as mine; Prof Nordelina Ilano, Director, Office for International Affairs of URS for her support to the Ph.D.Ed.M students; Dr Tran Thanh Van, the Dean of the Graduate School of Thai Nguyen ... Comparisons of respondent’s perception according to profile 25 Test homogeneity of variances in term of age 82 26 ANOVA analysis in term of age 83 27 Test homogeneity of variances in term of...
Ngày tải lên: 13/05/2014, 15:15
PERSONAL PROFILES OF THE ACADEMIC STAFFS AS PREDICTORS OF PROFESSIONAL CHARACTERISTICS: BASIS FOR STAFF DEVELOPMENT PROGRAM
... Dissertation of LAI VAN CHINH entitled PERSONAL PROFILES OF THE ACADEMIC STAFFS AS PREDICTORS OF PROFESSIONAL CHARACTERISTICS: BASIS FOR STAFF DEVELOPMENT PROGRAM Submitted in Partial Fulfillment of the ... Ability of supplying social services 47 13 Frequency and Average Weighted Mean of the Respondents' Profile in Terms of Ability of Ability of self-developing 48 14 Correlation of the professional ... in terms of age of respondents 49 15 Correlation of the Professional characteristics in terms of respondents’ Gender 50 16 Correlation of the Professional characteristics in terms of respondents’...
Ngày tải lên: 13/05/2014, 15:15
báo cáo hóa học: " Elimination kinetics of diisocyanates after specific inhalative challenges in humans: mass spectrometry analysis, as a basis for biomonitoring strategies" doc
... to the formula: yB + * sB and were 0.2 μg/L for 2,4-TDA, 0.2 μg/L for 2,6-TDA, 0.3 μg/L for 4,4’-MDA, 1.0 μg/L for 1,5-NDA, 1.0 μg/L 1,6 HDA and 1.0 μg/L for both IPDA isoforms The levels of the ... μg/L)and both isoforms of IPDA, (0.5 μg/L) The 1,7-HeDA and 3,3’-MDA were used as internal standards (to determine the recovery) For interpretation of the data, the peak areas of individual analyzed ... taking advantage of the known biotransformation of isocyanates to Page of respective biological amines and the detection of the parent aromatic amines after acid hydrolysis of urine samples [16,20,21]...
Ngày tải lên: 20/06/2014, 00:20