bacterial bioluminescence isolation and genetic analysis

... c-terpinene and is therefore designated ClcTS (Fig 3B), C62 and M34 both produced limonene and are designated Cl(+)LIMS1 and Cl(+)LIMS2, respectively (Fig 3C,E) and D85 produced b-pinene and is designated ... each other (91% identity) In a phylogenetic analysis the separate clustering within the tpsb family of C62 and M34 from B93 and D85 is clear (Fig 2) The B93 and D85 sequences group together with ... C62 (D) D85 and (E) M34 B93 mainly produces c-terpinene, C62 and M34 produce limonene and D85 mainly produces b-pinene Peak identities were confirmed using standards, whose mass spectra and retention...

Ngày tải lên: 08/03/2014, 23:20

... CCTTCGATGTCGGCTCT-3¢) and 28S-R (5¢-CTAA CCTGTCTCACGACGGT-3¢), and was used as an internal control probe in Northern hybridization for normalization of gcGLUT expression Phylogenetic analysis Phylogenetic analysis ... GLUT1 and GLUT3 (exons 3–8), and four in human GLUT2 (exons 7–10) and GLUT4 (exons 6–9) (Table 1) The codons for arginine (96) and valine (231) in gcGLUT (Fig 2) are split between exons and 5, and ... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and...

Ngày tải lên: 17/03/2014, 03:20

... their works Genetics and the Origin of Species and Systematics and the Origin of Species, perhaps to emphasizethat they were using genetics and systematics primarily to advance understandingof the ... remains the link between genetic divergence and mechanisms reproductive isolation (25) of Evenin systems amenableto formal genetics, like Drosophila, an understanding of the genetics of speciation ... isolation, but not which genetic changes have produced them The link between genetics and reproductive isolation is largely missing Recently, interest has increased in genetic divergenceof particular...

Ngày tải lên: 28/03/2014, 16:20

... proteins and therefore its role in HIV-1 pathogenesis To better characterize the oligomerization of Vpu and the domains for this, we have used genetic, biochemical and biophysical methods, and two ... electrophoretic and pull-down assays Vpu oligomerization based on gel electrophoretic and pull-down assays The full-length R5-Vpu protein and its transmembrane and cytoplasmic domains were synthesized and ... http://www.virologyj.com/content/4/1/81 Figure FRET analysis of Vpu interactions FRET analysis of Vpu interactions COS-1 and U2-OS cells were cotransfected with Vpu-ECFP and Vpu-EYFP expression vectors and FRET was performed...

Ngày tải lên: 18/06/2014, 18:20

... viruses Thus, we conducted detailed genetic analyses including homology analysis, phylogenetic analysis (using both the neighbor joining (NJ) and ML methods), and analysis of the pairwise distances ... detailed genetic analysis of the HN coding region in HPIV1 isolates from patients with ARI in Yamagata prefecture, Japan Methods Patients and isolation of HPIV1 A total of 182 throat and nasal ... Analyzer, Applied Biosystems, Foster City, CA) Phylogenetic analysis and calculation of pairwise distances by the NJ method Phylogenetic analysis of the nucleotide sequence of the HN coding...

Ngày tải lên: 18/06/2014, 18:20

... samples, performed RT-PCR and sequencing, participated in the genetic analysis and contributed to writing of the manuscript JL, JN and BP participated in fieldwork and in Page of (page number ... rodent samples KN performed genetic analysis of rodents GM and ÅL participated in the study coordination AV, HH and OV participated in the study design and coordination, and in drafting the manuscript, ... determined yet and, consequently, the Japanese genetic lineage was not presented on the phylogenetic tree (Fig 2) Within HOKV group, Chinese and Russian strains showed geographical clustering and formed...

Ngày tải lên: 20/06/2014, 01:20

... circovirus type in PMWS and PDNS Korean pigs between 1999 and 2006 Virus Res 2007, 129, 115-122 Andreyev VG, Wesley RD, Mengeling WL, Vorwald AC, Lager KM Genetic variation and phylogenetic relationships ... ethidium bromide The target bands were visualized using an ultraviolet lamp Sequencing and data analysis For sequencing ORF5, a 716 bp fragment of type II PRRSV and a 719 bp fragment of type ... 38 39 41 44 H , L , L and N (K/Q , F/I , S and K ) In the case 189 191 196 of the 3rd B-cell epitope (182∼200), I , R and Q 189 191 196 were frequently replaced by V , K and R in those groups...

Ngày tải lên: 07/08/2014, 23:22

... animal has in this population, 1/4 for each grandson and so on, and weighting by the size of the population, in such a way that AR will indicate its genetic contribution to the population The effective ... extracted according to standard procedures Ten equine microsatellites were chosen according to the ISAG (Comparison Tests, 1996): HTG4 and HTG6 !3!, HTG8 and HTG10 !15!, VHL20 [24] and HMS2, HMS3, HMS6, ... compute inbreeding values (overall and by subpopulations), number of known generations and effective number of founders and of parents per generation (tables I and 77) The second block of information...

Ngày tải lên: 09/08/2014, 18:21

... all test-days from DIM 25 to 174 Standards errors between 0.011 and 0.034 Standards errors between 0.029 and 0.119 for genetic correlations and between 0.012 and 0.075 for environmental correlations ... lamb, early lambing, and lambing in 1994, respectively 2.3.2 Estimation of genetic parameters for SCC (on-farm data) The genetic parameters for the different SCC traits and the genetic correlation ... 441–600, and 601– 920 days); Lambsk = fixed effect of the number of suckled lambs k (2 levels: vs or more); al = random genetic effect of animal l; eijkl = random residual effect In the second analysis, ...

Ngày tải lên: 09/08/2014, 18:21

... Patient population and genetic analysis We carried out genetic analysis of 13 HIV-1 envelope sequences from Northern India Nine unrelated and mother-child pairs (Pair 1, D & E 57 and Pair 2, D & ... to various genetic subtyping tools (Phylogenetic Analysis, RIP 3.0, Viral Genotyping Tools and Rega Subtyping) This analysis indicated that of 13 were related to subtype C, one B and the rest ... Biotech WI, USA) and sequenced in both directions using T7 and SP6-specific primers The sequence from one representative clone from each sample was used to carry out phylogenetic analysis and sequence...

Ngày tải lên: 10/08/2014, 05:21

... subtypes B and C and with probably other subtypes as well The analysis of intra-subtype divergence (genetic distance from Page of Indian consensus sequence) and diversity (intra-subtype genetic ... is no data available on HIV-1 subtype C RRE genetic and functional characteristics In the present study, we present in-depth genetic and functional analysis of RRE sequences from a cohort of 13 ... protein and subjected to EMSA as described earlier [18] Results and discussion Analysis of RRE nucleotide sequences 83 sequences of subtype B (from USA, Japan, Mayanmar, France and Brazil) and 83...

Ngày tải lên: 10/08/2014, 05:21

... [33,34], and identification of the convergent and divergent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding ... suppression subtractive hybridization and microarray analysis J Exp Bot 2007, 58:507-520 Sahi C, Agarwal M, Reddy MK, Sopory SK, Grover A: Isolation and expression analysis of salt stress-associated ... enriched population of the overexpressed and newly induced transcript messages, TA1 and T2R were considered as 'Tester-A' and “Tester-B', respectively, and the C as the 'Driver' The opposite was...

Ngày tải lên: 12/08/2014, 03:20

... research, collected, analyzed and interpreted the data, and drafted and revised the manuscript NTHT carried out the collection, analysis and interpretation of the data DTND and MK contributed to the ... children–a prospective CSF analysis study J Trop Pediatr 2000, 46:331-334 58 Schwarz S, Bertram M, Schwab S, Andrassy K, Hacke W: Serum procalcitonin levels in bacterial and abacterial meningitis Crit ... concern for meta -analysis, the potential presence of this bias was identified using a funnel plot and Egger test [33] If publication bias was found, the trim and fill method of Duvall and Tweedie...

Ngày tải lên: 14/08/2014, 07:21

... physical and genetic maps of Trypanosoma brucei brucei Comparison Comparison with the physical and genetic maps of Trypanosoma brucei brucei The genetic maps of T b brucei isolate TREU 927 and T ... position on the genetic maps Hot and cold spots are defined as threefold more or less recombination than average for each genetic map and indicated against the physical map by red and blue bars, ... microsatellite mutations occur may enhance our understanding of the evolution and stability of such markers and their usefulness in genetic analysis of T brucei populations Few such estimates exist...

Ngày tải lên: 14/08/2014, 20:22

Ngày tải lên: 13/11/2014, 09:37

... gene and its related functional and genetic studies 21 1.2.3 ABCB4 (MDR3) gene and its related functional and genetic studies 26 1.2.4 An important CYP3A cluster: the CYP3A4, CYP3A5, CYP3A7 and ... MDR1, MDR3 genes and the CYP3A cluster are introduced Previous genetic and pharmacogenetics studies on these candidate genes are also reviewed in this section Finally, the objectives and significance ... the non-random association between the genetic variations, which plays pivotal role in many genetic studies including Pharmacogenetics Linkage Disequilibrium rises and decays by mutation and recombination...

Ngày tải lên: 15/09/2015, 17:10

... bars represent mm and Insel 2002; Goodson and Bass 2001) On the other hand, the main role of vasopressin is maintenance of blood osmolarity and volume homeostasis (Orloff and Handler 1967) Oxytocin ... rats and Fugu, paving the way for comparative genetics analysis of the enhancer The transgene was introduced in a cosmid, and the authors did not perform deletion analysis, so there is no genetic ... sequences, and thereby map TF binding sites involved in specific expression, again, using comparative genetics 31 Chapter Materials and Methods 32 Materials and Methods 2.1 Isolation and sequencing...

Ngày tải lên: 15/09/2015, 22:13

... out in Chapter A genetic algorithm uses the RSM models and the non linear circuit model of the IPMSM The genetic algorithm is described and the choice of the genetic operators and the parameters, ... (2.3) Vq = RIq + dλd + ωλd dt (2.4) where Vd and Vq are the d- and q-axis voltages Id and Iq are the d- and q-axis currents λd and λq are the d- and q-axis flux linkages R is the armature resistance ... performance and possibly adjust the design Different approaches centered on FEM were proposed for PMSM design and optimization Bianchi [35], Chung [37] and Sim [38] combined FEM and Genetic Algorithms...

Ngày tải lên: 04/10/2015, 15:45