b1 ngf and bdnf levels in bal fluid
Báo cáo y học: " Lymphocyte apoptosis in murine Pneumocystis pneumonia Xin Shi, Nicole J LeCapitaine, Xiaowen L Rudner, Sanbao " potx
Ngày tải lên :
12/08/2014, 14:20
... stained with Giemsa stain (Diff-Quick, Dada Behring, Newark, DE) BAL cells and spleen cells (50,000 in number) were centrifuged (500 rpm for min) onto glass slides, and stained with Giemsa stain ... staining of annexin V and intracellular activity of caspases 3, 8, and In control mice, numbers of apoptotic cells began to increase in lavaged CD4+ lymphocytes by week 2, were significantly increased ... observed within alveolar macrophages in lung sections from infected animals In CD4-depleted mice, apoptosis was observed in recruited CD8+ and CD19+ lymphocytes beginning at week and persisting to...
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Báo cáo khoa học: "Density of CD4(+) and CD8(+) T lymphocytes in biopsy samples can be a predictor of pathological response to chemoradiotherapy (CRT) for rectal cancer" ppt
Ngày tải lên :
09/08/2014, 09:20
... peroxidase-conjugated streptavidin for 30 min, and developed by immersion in 0.01% H O and 0.05% diaminobenzidine tetrahydrochloride for Light counterstaining with Mayer’s hematoxylin was performed The ... Japan, and the Ministry of Health, Labor and Welfare of Japan Authors’ contributions JK participated in the study design and data retrieval and analysis KY, KK, ES participated in immunostaining and ... number and distribution of T cells in biopsy samples before CRT were evaluated with immunostainig withm Abs against CD4 and CD8 As shown in Figure 1, both CD4 and CD8 were clearly stained in the...
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Báo cáo hóa học: " Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients" pdf
Ngày tải lên :
18/06/2014, 16:20
... between age and sjTRECs numbers Figure Comparison of the sjTRECs levels in patients with CML and healthy individuals (HI) A: The sjTRECs levels in PBMCs; B: The sjTRECs levels in CD4+ and CD8+ T ... measured in PBMCs from all 48 cases, and CD4+ and CD8+ T cells from 19 cases TRBV sjTRECs were determined in PBMCs, CD4+ and CD8+ T Quantitative detection of δRec-ψJα sjTRECs in DNA from PBMCs and ... probe and PCR Buffer including 4.5 mM MgCl2 After the initial denaturation at 95°C for min, 45 cycles consisting of 95°C for 30 sec and 67°C for were performed If no TRECs were detected in a sample,...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
Ngày tải lên :
09/08/2014, 01:21
... also expanded in WG and is correlated with organ involvement [25–28] These findings support our designation of CD45RA+ T cells expressing the inducible inflammatory chemokine receptors CCR5 and CCR3 ... chemokine receptors such as the inducible inflammatory chemokine receptors CCR5 and CCR3 – along with selectins and adhesion molecules – have a pivotal role in effector memory T cell trafficking into ... migration of distinct Th1-type and Th2-type T cell populations into granulomatous lesions and vasculitic areas, resulting in differences in tissue cytokine patterns found in localized WG and generalized...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
Ngày tải lên :
11/08/2014, 08:20
... between CD4 and CD8 cell counts and mRNA levels of CD4 and CD8 could be determined by QC-RT-PCR There was also no correlation between CD4 and CD8 mRNA levels and the number of cells expressing these ... GM and Lifson JD High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR Science 1993, 259(5102):1749-1754 McKinney RE Jr and Wilfert CM Lymphocyte subsets in ... Our results indicate that there is no correlation between CD4 and CD8 mRNA levels and numbers of cells expressing these proteins on their surface The formation of CD4gp160 complexes in intracellular...
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Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot
Ngày tải lên :
13/08/2014, 01:20
... illustrated In the remaining three categories, butanoate and fatty acid metabolism were top listed in carbohydrate and lipid metabolism The valine, leucine, and isoleucine degradation in nitrogen ... cycle and apoptosis related; (2) cytotoxicity, complement activation, and cell signaling; (3) interleukin and interferon responses; and (4) cytoskeleton and cell adhesion In the cell cycle and ... up-regulated genes encoding for proteins that generally prevent the cell cycle, including (1) the protein kinases WEE1 and MYT1, which inactivate the complex CDK1-cyclin B pivotal in regulating G2 to M transition;...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
Ngày tải lên :
13/08/2014, 05:22
... (in VIR) and CD27 (in LTNP), while increases in CD21 2b1 (in LTNP and VIR) did not reach significance (p = 0.0745–0.0776) CD38 was significantly upregulated in the VIR group compared with BDL and ... cells providing significant discrimination between patient groups, with relative changes in CD antigen binding in the former vs the latter denoted by "+" and "-" to indicate increase and decrease, ... cells providing significant discrimination between patient groups, with relative changes in CD antigen binding in the former vs the latter denoted by "+" and "-" to indicate increase and decrease,...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
Ngày tải lên :
13/08/2014, 09:21
... directly binding to its sugar export site [34,35] In the presence of CytB, H2RBDEGFP binding was significantly decreased, strongly suggesting that CytB binding to Glut-1 directly inhibits binding of ... immunoblotting as described below) In contrast to a lack of extracellular or intracellular staining by mAb1418, both H1RBD and H2RBD interacted with extracellular Glut-1 resulting in significantly ... fluorescence intensity bindings to human erythrocytes as compared to either the 293T or Jurkat cell lines (Fig 2A) Moreover, the level of H1RBD and H2RBD binding, but not that of mAb1418 staining, correlated...
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Báo cáo y học: " Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells" pdf
Ngày tải lên :
12/08/2014, 18:22
... lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells Eur J Immunol 1994, 24(5):1097-101 Boilard E, Surette ME: Anti-CD3 and concanavalin A-induced human T ... vivo suppression of NF-B and preservation of IB by interleukin-10 and interleukin-13 J Clin Invest 1997, 100:2443-2448 Boilard E, Surette ME: Anti-CD3 and concanavalin A-induced human T cell proliferation ... recruitment and accumulation of inflammatory cells into the tissues and, most importantly, suggest their fundamental role in cell-to-cell communication and inflammation control Indeed, our findings...
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Xác định sơ bộ giá trị phần trăm, tuyệt đối của tiêu chuẩn quần thể Lympho (T CD3, T CD4, T CD8, B, NK) ở nhóm người bình thường tại thành phố Hồ Chí Minh bằng máy Fascalibur docx
Ngày tải lên :
25/03/2014, 03:22
... Pasteur Institute-HCMC and then compare the results with those obtained in a group of healthy adults Iranian population The samples obtained from the 47 healthy adults donors were stained with ... lymphocyte subsets in a healthy iranian population Shahpour Shahghasempour, Mitra Gerami, Zinat Entezami Archives of Iranian Medecine, 2001; 4(2): 80-83 Age and sex related changes in lymphocyte subpopulation ... SUBPOPULATION IN HEALTHY ADULTS DONORS OF HO CHI MINH CITY VIET NAM The primary objective of this study was to establish reference ranges for the lymphocyte subsets (T CD3, T CD4, T CD8, B, NK) in the...
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Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot
Ngày tải lên :
30/03/2014, 20:20
... gradients and kept with interleukin (100 UÆmL)1) and phytohemagglutinin (5 lgÆmL)1) in RPMI 1640 containing 10% fetal bovine serum and 100 IU penicillin/streptomycin CHO-TRVb [human transferrin receptor ... Hut78, KG1a, and HeLa, and freshly isolated nonactivated PBLs and phytohemagglutinin/interleukin 2-activated PBLs were stained with soluble scFvs 18 and 92 The cells were also stained with CXCR4 ... removed, and 107 cells were washed once with NaCl/Pi by centrifugation RPMI 1640 lacking cysteine and methionine and supplemented with dialyzed 10% fetal bovine serum, 100 IU penicillin/streptomycin,...
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Báo cáo hóa học: "Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients" ppt
Ngày tải lên :
18/06/2014, 16:20
... BD Pharmingen (fluorescein isothiocyanate anti-CD3 and -CD45RA, peridin-clorophyll protein-Cy5.5 anti-CD8 and allophycocyanin-H7 anti-CD4), BioLegend (phycoerythrin antiCD25 and peridin-clorophyll ... obtained by extrapolating the respective sample quantities from the standard curve obtained by serial dilutions (106, 105, 104, 103, 102, and 10) of a linearized plasmid DNA, containing three inserts ... threshold number obtained by PCR amplification of signal joints, which are sequences contained into KRECs, and the cycle threshold number obtained after amplification of coding joints, which are sequences...
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Báo cáo y học: "Impact of cytokines and T lymphocytes upon osteoclast differentiation and function" pot
Ngày tải lên :
09/08/2014, 10:20
... differentiation, and its central role in regulating bone mass is exemplified by individuals with high bone mass phenotypes having activating mutations in the Wnt pathway Wnt signalling is also involved in ... to inhibit osteoclast formation and when administered together at synergistically low doses both GM-CSF and IFN-γ are produced [8] Leptin has both central and peripheral roles in influencing ... cytokine repertoire Competing interests The author declares that he has no competing interests References Romas E, Gillespie MT, Martin TJ: Involvement of receptor activator of NFkappaB ligand and...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
Ngày tải lên :
12/08/2014, 04:21
... cells infected with MN virus (containing ~8 ng/ml of p24gag) or from VCRT C17 cells infected with BAL virus (containing ~1 ng/ml of p24gag) were collected and used for infection of human PBMC and ... derived clones expressing hCD4 and hCCR5 infected with HIV-1 BAL isolate; (C), VCRT parental cell line and the derived C17 clone expressing human CD4 and CCR5 infected with HIV-1 BAL isolate Page ... collaborators[19] detected viral cDNA in spleen and brain of all the infected animals months post-infection, showed increase in mortality (17%), and found morphological changes in cells of the CNS We have...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
Ngày tải lên :
12/08/2014, 04:21
... cells infected with MN virus (containing ~8 ng/ml of p24gag) or from VCRT C17 cells infected with BAL virus (containing ~1 ng/ml of p24gag) were collected and used for infection of human PBMC and ... derived clones expressing hCD4 and hCCR5 infected with HIV-1 BAL isolate; (C), VCRT parental cell line and the derived C17 clone expressing human CD4 and CCR5 infected with HIV-1 BAL isolate Page ... collaborators[19] detected viral cDNA in spleen and brain of all the infected animals months post-infection, showed increase in mortality (17%), and found morphological changes in cells of the CNS We have...
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Báo cáo sinh học: " Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes" ppsx
Ngày tải lên :
14/08/2014, 19:22
... penicillin-streptomycin and glutamine, and induced to differentiate into DCs with 20 ng/ml recombinant mouse granulocyte macrophage colony-stimulating factor (GM-CSF) and ng/ml recombinant mouse interleukin (IL)-4 ... packaging vectors, FC and BDS were involved in the in vitro characterization of the vector, GF produced and analyzed the dendritic cells, MB was involved in drafting and critical revising the ... minimal, self-inactivating vector with both the U3 LTR domains deleted and devoid of all accessory and structural proteins except ψ, a 120 nt stretch at the of 5'-end gag containing the domains...
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Báo cáo khoa học: " Early changes of CD4-positive lymphocytes and NK cells in patients with severe Gram-negative sepsis" ppt
Ngày tải lên :
13/08/2014, 03:20
... 36.5–38.4°C = points, 38.5–38.9°C = point, and ≤36°C or ≥39°C = points; white blood cell count, 4,000–11,000/μl = points, 11,000/μl = point, and >11,000 points and >10% bands = points; pO2/FiO2, ... Calandra T, Cohen J, International Sepsis Forum Definition of Infection in the ICU Consensus Conference: The international sepsis forum consensus conference on definitions of infection in the intensive ... triggering receptor expressed on myeloid cells-1 as an antiinflammatory mediator in sepsis Intensive Care Med 2006, 32:237-243 Pinson AG, Philbrick JT, Lindbeck GH, Schorling JB: Fever in the clinical...
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Báo cáo y học: "Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo" doc
Ngày tải lên :
13/08/2014, 05:21
... that interactions with SH3 domain containing ligands are dispensable for CD4+ T cell depletion In SIV-infected macaques, the relevance of the SH3-binding motif in Nef is controversial [51-53] In ... presentation of the data shown in A and B Boxes indicate the upper and lower quartile (box borders), median (line within the box) and the maxima and minima of each data set (vertical lines) 1.0) [37] The ... lower right quadrant), reflecting bystander killing in response to virus infection (Fig 5E) This increase in death of p24bystander cells was less pronounced in cultures infected with HIV-1Δnef (19.8%...
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Báo cáo y học: " Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes" pdf
Ngày tải lên :
13/08/2014, 09:20
... lowering rates of transmission, given the relative inefficiency of infection [35,36] The effect of CQ on DC-SIGN binding in vivo remains to be determined, but if the DC-SIGN binding is indeed ... by standard FicolHypaque density centrifugation, pooled and frozen in multiple vials After thawing, PBMCs were activated with phytohemagglutinin (2 μg/ml) and cultured in RPMI medium containing ... cervicovaginal mucosa after intravaginal inoculation and infects intraepithelial dendritic cells J Virol 2000, 74:6087-6095 Pope M, Gezelter S, Gallo N, Hoffman L, Steinman RM: Low levels of HIV-1 infection...
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Báo cáo y học: " Decrease of CD4-lymphocytes and apoptosis of CD14-monocytes are characteristic alterations in sepsis caused by ventilator-associated pneumonia: results from an observational study" ppt
Ngày tải lên :
13/08/2014, 19:20
... CD(16+56), CD19 and with the protein annexin-V and propidium iodine (PI) (Immunotech, Marseille, France), and incubated for 15 minutes in the dark Fluorocolours used were fluorescein isothiocyanate ... annexinV(FITC)/CD4(PE)/PI (PC5), and annexin-V(FITC)/antiCD8(PE)/PI(PC5) Cells that stained positive for annexin-V and negative for PI were considered apoptotic In an attempt to explain our findings, ... intraabdominal infection was made in patients with temperature above 38°C or below 36°C, leukocytosis (WBC >12,000 cells/μl) and radiological findings consistent with an intraabdominal infection...
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