... again and a HSA standard curve (1.5-1000 μg/ml) and samples were added and incubated at 4C for hours Following incubation, the plate was washed and a rabbit anti-HSA antibody conjugated to HRP was ... assay Total protein levels of lung homogenates were measured using a commercially available BCA assay from BioRad using a Human Serum Albumin (HSA) standard curve Limit of detection for HSA was μg/ml ... serum albumin Biochim Biophys Acta 1995, 1247(1):12-6 Soriani M, Pietraforte D, Minetti M: Antioxidant potential of anaerobic human plasma: role of serum albumin and thiols as scavengers of carbon...
Ngày tải lên: 12/08/2014, 13:22
... phosphatase–anti-alkaline phosphatase (APAAP) method (see later) Immunohistochemical double-labeling (APAAP method) Double-labeling was performed using the APAAP method, with monoclonal antibody against ... polylinker site of bluescript SK+ (Stratagene, CA, USA) Antisense and sense RNA probes were transcribed by T3 and T7 RNA polymerase using a commercially available RNA transcription kit, according to ... that PR-3 mRNA, the target antigen for c-ANCA, is expressed by normal lung parenchymal cells and is upregulated in lung tissue of WG patients PR-3 mRNA may therefore contribute to lung damage...
Ngày tải lên: 09/08/2014, 03:24
Báo cáo y học: "Impaired expression of mitochondrial and adipogenic genes in adipose tissue from a patient with acquired partial lipodystrophy (Barraquer-Simons syndrome): a case report" pot
... presentation A biopsy sample of subcutaneous adipose tissue was taken from the arm Control values of gene expression in adipose tissue were obtained from the analysis of biopsies of subcutaneous adipose ... the analysis and interpretation of data in relation to renal and liver function, cytogenetic analysis and blood analysis EGA and ALA carried out muscle analysis JCD performed the analysis and ... upper part of the body Other alterations, such as nephropathy or central nervous system abnormalities, are often, but not always, present [1] Patients without any associated anomalies or medical...
Ngày tải lên: 11/08/2014, 21:22
Báo cáo y học: "Identification of target antigens of antiendothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach" potx
... group of patients with ANCA-associated vasculitis, two-thirds of the patients had active disease as assessed by a Birmingham Vasculitis Activity Score (BVAS) >3 in the absence of treatment, and ... diseases [10,35] AECAs have been associated with disease activity in patients with vasculitis, particularly in those with anti-ANCA-associated vasculitis, Takayasus arteritis or GCA [15], although ... interactions Dashed lines indicate indirect interactions Arrows indicate stimulation ABCA2: ATPbinding cassette, subfamily A, (ABC1), member 2; ACTB: actin, b; ACTG1: Rộgent et al Arthritis Research...
Ngày tải lên: 12/08/2014, 17:22
Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
... SUMA software: subsite mapping of amylases This software calculates the apparent binding energies on the basis of the measured bond cleavage frequencies The calculations are based on the equation: ... data of Table Fig Subsite maps for porcine pancreatic a- amylase (PPA) The solid bars are related to CNP-modified maltooligosaccharide substrates [8] and the open bars depict the subsite map with ... followed by a barrier subsite The action of barley a- amylase on amylose as substrate was also studied earlier by MacGregor & MacGregor [20] and a ‘6 + model’ was proposed Our calculations seem...
Ngày tải lên: 08/03/2014, 09:20
báo cáo hóa học: " Impact of a child with congenital anomalies on parents (ICCAP) questionnaire; a psychometric analysis" pptx
... Primary anomalies Abdominal wall defect Congenital diaphragmatic hernia Small intestinal anomaly Oesophageal atresia Anorectal malformation Hirschsprung's disease Miscellaneous Congenital anomalies ... the children's biographic characteristics, primary CA, and medical care as well as parental biographic and demographic variables used in the data analysis Severity of disease was derived from ... syndromal pattern of malformation may even heighten parental insecurity, notably in the case of MCA While empirical research has evaluated parental burden experienced one year after the birth of a...
Ngày tải lên: 18/06/2014, 19:20
báo cáo hóa học:" Impact of a child with congenital anomalies on parents (ICCAP) questionnaire; a psychometric analysis" pot
... Primary anomalies Abdominal wall defect Congenital diaphragmatic hernia Small intestinal anomaly Oesophageal atresia Anorectal malformation Hirschsprung's disease Miscellaneous Congenital anomalies ... the children's biographic characteristics, primary CA, and medical care as well as parental biographic and demographic variables used in the data analysis Severity of disease was derived from ... syndromal pattern of malformation may even heighten parental insecurity, notably in the case of MCA While empirical research has evaluated parental burden experienced one year after the birth of a...
Ngày tải lên: 20/06/2014, 16:20
Báo cáo toán học: "RHOMBUS TILINGS OF A HEXAGON WITH TWO TRIANGLES MISSING ON THE SYMMETRY AXIS" pdf
... Krattenthaler, An alternative evaluation of the Andrews–Burge determinant, in: Mathematical Essays in Honor of Gian-Carlo Rota, B E Sagan, R P Stanley, Progress in Math., vol 161, Birkh¨user a ... number of lattice paths with positive horizontal and negative vertical steps from (a, b) to (c, d) equals c a +b d Therefore, the number of families of noninterb−d secting lattice paths (equivalently, ... generality, we can assume that s ≤ n because both the final result (see Theorem 1) and the number of rhombus tilings of the original graph are invariant under the transformation s → n − s Most of...
Ngày tải lên: 07/08/2014, 06:20
Báo cáo khoa học: "Retrieval of an embolization coil accidentally dislodged in the descending aorta of a dog with a patent ductus arteriosus" doc
... desu neeb evah steksab ralucsav llams dna serans yrruC ,)ASU ,ekaL raeB etihW ,anevorciM( eranS kcenesooG ztalpmA eht ,enicidem namuh nI elbaliava era sloot laveirter etairporppa taht laitnesse ... gnipsarg specrof ydob ngieroF )B( ,troper esac siht ni desu pit lian eriw-eerht htiw specrof ydob ngieroF )A( lioc giF lioc noisulcco degdolsid eht yb desuac atroa lanimodba eht ta noitazilobme ... ypocsoroulf lanimodba eht fo noitcejorp laretaL siht fo etar sseccus eht etad oT ADP llams a htiw sgod gnuoy ni ymotocaroht ot evitanretla evitceffe dna elpmis a si ,lioc noitazilobme na gnisu ,ADP a fo...
Ngày tải lên: 07/08/2014, 20:23
Báo cáo khoa học: "Malignant gastrointestinal stromal tumor presenting with hemoperitoneum in puerperium: report of a case with review of the literature" ppt
... di Paola E, Dimitrijevic S, Martens M, Webb A, Sciot R, Van Glabbekc M, Silberman S, Nielsen OS: Safety and efficancy of Imatinib (STI571) in metastatic gastrointestinal stromal tumours: A phase ... [Sanjay et al 2004] The hypothesis that GISTs originate from the primitive stem cell that can differentiate toward the interstitial cells of Cajal, has been advocated Interstitial cells of Cajal ... generalized peritonitis: report of a case Surg Today 2001, 31:350-354 Maeda H, Yamagata A, Nishikawa S, Yoshinaga K, Kobayashi S, Nishi K, Nishikawa S: Requirment of c-kit for development of intestinal...
Ngày tải lên: 09/08/2014, 03:22
Báo cáo y học: "Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome" pptx
... gland B cells of a patient with SS compared with B cells obtained from the peripheral blood of the same patient This patient manifested increased titers of autoantibodies (anti-Ro and anti-La), ... years at the time of analysis The patient expressed elevated titers of anti-52 kDa Ro(SS -A) and anti-52 kDa La(SS -B) antibodies, had marked hypergammaglobulinemia, and was rheumatoid factor (RF) ... plasma and memory B cells accumulate in the parotid gland but cannot clarify the origin of these cells Whatever the primary aberration in the induction of the salivary inflammation, one abnormality...
Ngày tải lên: 09/08/2014, 03:24
Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... DNA profiles of bacterial species were obtained from tissue samples that gave weak PCR signals (Table 2) Bacterial 16S rDNA sequences identified in synovial tissue samples A broad range of DNAs ... Taq Buffer (Takara Ex taq, Otsu, Shiga, Japan), 0.2 mmol/l of each primer, 2.5 mmol/l of each DNTP, mmol/l MgCl2 and 1.25 units of Takara Ex Taq DNA polymerase (Takara Ex taq, Otsu, Shiga, Japan ... fruste' of ReA Arthritogenic bacterial DNA and RNA from Chlamydia trachomatis, Chlamydophila pneumoniae, and Yersinia pseudotuberculosis have been detected by PCR in synovial samples from patients with...
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Cytokines in the colon of a patient with Behçet’s disease" ppt
... management, and advances in treatment Nat Clin Pract Rheumatol 2007, 3:148-155 van Laar JA, Missotten T, van Daele PL, Jamnitski A, Baarsma GS, van Hagen PM: Adalimumab: a new modality for Behçet’s ... immunopathology in Behçet disease and psoriatic arthritis Arthritis Res Ther 2009, 11:R17 Dik WA, Nadel B, Przybylski GK, Asnafi V, Grabarczyk P, Navarro JM, Verhaaf B, Schmidt CA, Macintyre EA, van Dongen ... Arthritis Research & Therapy Vol 11 No Kappen et al in colonic tissue of a BD patient, IFN-γ, TNF-α, and IL-1 7A appear to be key cytokines, even in a treated patient Competing interests The authors...
Ngày tải lên: 09/08/2014, 14:22
Báo cáo y học: "A long-term follow-up of a girl with dilated cardiomyopathy after mitral valve replacement and septal anterior ventricular exclusion" docx
... decreased from the anterior part of the IVS and anterior wall of the LV on cardiac scintigraphy (Arrow: Anterior wall of LV, Arrowhead: Anterior part of IVS) Randas Batista et al described techniques ... functional class, who not respond to medical therapy, are candidates for heart transplantation In addition to the shortage of available organs, there are legal, economical, ethical and technical problems ... assistant professor and a pediatric cardiologist in charge in a pediatric ward and an outpatient clinic of Kyoto university hospital TI is an associate professor in the department of cardiovascular...
Ngày tải lên: 10/08/2014, 10:20
Báo cáo khoa học: "Pulmonary intravascular lymphoma diagnosed by 18fluorodeoxyglucose positron emission tomography-guided transbronchial lung biopsy in a man with long-term survival: a case report" pot
... who had pulmonary intravascular lymphoma and no computed tomography abnormality We were able to make an antemortem diagnosis of pulmonary intravascular lymphoma by transbronchial lung biopsy according ... Thorac Imaging 2009, 24:231-233 Yamagata T, Okamoto Y, Ota K, Katayama N, Tsuda T, Yukawa S: A case of pulmonary intravascular lymphomatosis diagnosed by thoracoscopic lung biopsy Respiration ... Kitanaka A, Kubota Y, Imataki O, Ohnishi H, Fukumoto T, Kurokohchi K, Tanaka T: Intravascular large B- cell lymphoma with FDG accumulation in the lung lacking CT/(67)gallium scintigraphy abnormality...
Ngày tải lên: 10/08/2014, 23:21
Báo cáo y học: " Dual paraneoplastic syndromes in a patient with small cell lung cancer: a case report" pdf
... Manuals Online Medical Library [http://www.merck.com/ mmpe] Biller BM, Grossman AB, Stewart PM, Melmed S, Bertagna X, Bertherat J, Buchfelder M, Colao A, Hermus AR, Hofland LJ, Klibanski A, Lacroix ... contributions KC collected all of the patient data and wrote the initial draft of the manuscript SW checked all of the data for accuracy and did considerable Coners et al Journal of Medical Case ... same side of the chest) Patients with limited-stage disease have a median survival approaching two years and a 14% five-year survival rate Patients with extensive disease have a median survival...
Ngày tải lên: 10/08/2014, 23:22
Báo cáo y học: " Prenatal exposure of a girl with autism spectrum disorder to ‘horsetail’ (Equisetum arvense) herbal remedy and alcohol: a case repor" ppsx
... statistical data, however it raises awareness that FAS could be a risk factor that should be evaluated by physicians Neonatal hyperbilirubinemia Not a risk factor associated with ASD Children with any ... immigration/mother born abroad Increased risk of ASD according to region and ethnicity; more risk in Caribbean and African-American populations Daily smoking in early pregnancy The risk of autism ... defects Associated with a near twofold increased risk for autism overall Chlorinated solvents and heavy metals Association between autism and estimated concentrations in ambient air around birth...
Ngày tải lên: 11/08/2014, 00:23
báo cáo khoa học: "Long-term survival of a woman with well differentiated papillary mesothelioma of the peritoneum: a case report and review of the literature" docx
... was maintained on combination capecitabine and gemcitabine therapy and had stable disease as assessed by CT scans However, in early 2003 she was found to have declining renal function and was ... lined by a simple uniform cuboidal epithelium, with little to no nuclear atypia or mitoses The amount of fibrosis present can be variable and psammoma bodies can also be found [4,8] Areas of invasion ... chemotherapy with substantial associated morbidity, we believe on the basis of the fact that her tumor was thought originally to be an ovarian-derived tumor or primary peritoneal carcinoma, and was later...
Ngày tải lên: 11/08/2014, 02:21
báo cáo khoa học: "Oxygen supplementation facilitating successful prosthetic fitting and rehabilitation of a patient with severe chronic obstructive pulmonary disease following trans-tibial amputation: a case report" potx
... unilateral trans-tibial amputation 40-60%, unilateral trans-femoral amputation 90 to 120%, bilateral trans-tibial amputation 60-100% and bilateral transfemoral amputation > 200% Patients with ... husband, lived in a wheelchair-accessible bilevel home and was using a wheelchair for ambulating long distances and was mobilized with a walker for short distances Her physical examination revealed ... prosthetic ambulation Case Presentation We report a case of a 67-year-old aboriginal woman who was admitted to the rehabilitation ward In March 2007, the patient underwent right femoral popliteal artery...
Ngày tải lên: 11/08/2014, 02:22
Báo cáo khoa hoc:" Perfluorodecaline residue in the anterior chamber of a patient with an intact crystalline lens: a case report" ppt
... operation (a and b) Giant retinal tear with shallow detached retina involving the macula in the right eye (c) Reattachment of retina after vitrectomy sis, but could not obtain these results as ... patient refused (4) After our attempt to remove the perfluorodecaline in the anterior chamber with a 27G needle failed, an anterior chamber wash was performed with balanced salt solution via ... Perfluorodecaline may also have a role in the etiology of some changes, such as corneal oedema and deep corneal vascularization in the area of perfluorodecaline-endothelial contact It is known that corneal...
Ngày tải lên: 11/08/2014, 10:23