b cells in human systemic lupus erythematosus

... Olson JM, Jedrey CM, Jacobs KB, Kimberly RP, Neas BR, Rich SS, Behrens TW, Harley JB: Genome scan of human systemic lupus erythematosus by regression modelling Evidence of linkage and epistasis at ... Kearns GM, Messner RP, King RA, Rich SS, Behrens TW: Genome screening in human systemic lupus erythematosus: results from a second Minnesota cohort and combined analyses of 187 sib-pair families Am ... harbour the disease gene Linkage disequilibrium mapping can be carried out either by directly testing potential candidate genes or by using microsatellite markers mapping to a region of linkage...

Ngày tải lên: 09/08/2014, 03:24

... for certain anti-histone murine mAbs, whose binding activity is increased by DNA [22,26] In human SLE sera, such autoantibodies probably exist but their identification remains elusive because ... activity of 4B7 mAb Binding of rhNPM to cardiolipin and effects of nucleophosmin on cardiolipin-binding activity of 4B7 mAb (a) Cardiolipin was coated on plastic plates and incubated with various ... 50 µl of bovine heart CL (10 µg/ml) in absolute alcohol The plates were incubated overnight at 4°C to allow the ethanol to evaporate After blocking of non-specific binding sites by incubation with...

Ngày tải lên: 09/08/2014, 07:20

... patients at the beginning of the study, using the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI)20 and Systemic Lupus International Collaborating Clinics/ACR-Damage Index (SLICC/ACR-DI) ... SLEDAI – Systemic Lupus Erythematosus Disease Activity Index, SLICC/ACR-DI – Systemic Lupus International Collaborating Clinics/ACR Damage Index 220 EM0000 Rev Bras Reumat 49(3).indd 220 Bras J ... antispermatozoid antibodies were performed at the beginning of the study in the Human Reproduction Center of HC-FMUSP These were determined by direct Immunobead test which uses reagents containing rabbit immunoglobulins...

Ngày tải lên: 13/02/2014, 16:20

... CI, confidence interval; F, phenylalanine; MBL, mannan-binding lectin; MBL-low, MBL genotype conferring a low serum concentration of MBL; OR, odds ratio; R, arginine Competing interests None ... intermediate; MBL, mannan-binding lectin; R, arginine; V, valine from a homogeneous Caucasian population, although a possible bias exists in the fact that the controls used were blood donors, which principally ... processing in patients with systemic lupus erythematosus In vivo imaging and clearance studies J Clin Invest 1992, 90:2075-2083 Sullivan KE, Wooten C, Goldman D, Petri M: Mannose-binding protein...

Ngày tải lên: 09/08/2014, 01:24

... germinal centre (GC) A (b) Staining for CD20 FDC staining suggests two possible GCs within close proximity, but B- cell staining shows no clear demarcation (c) Anti-FDC staining for GC B (d) staining ... distinct areas of staining for FDC and one large area of B cells encompassing both FDC regions Very few T cells could be seen within GC A and B, and no plasma cells could be seen at all within ... clones of B cells are proliferating and mutating in the splenic GCs and B- cell clusters Serine codon usage There is evidence for a bias toward serine codon usage within immunoglobulin variable genes...

Ngày tải lên: 09/08/2014, 01:21

... epratuzumab will be shown to improve SLE clinically, then the lack of biological markers of its effects may in fact be a drawback to its rational use Because even the fall in B cells in peripheral blood ... true of rituximab [1] It is unfortunate that there is only limited preclinical information available on the signaling mediated by epratuzumab binding to CD22 on various subsets of B cells [9] Several ... is that combining epratuzumab with rituximab, although not increasing the overall clinical response rate above what is seen with rituximab alone, may lead to a substantially higher number of complete...

Ngày tải lên: 09/08/2014, 08:22

... not observe any significant inhibition of epratuzumab binding on monocytes after incubation with unlabeled F(ab’)2 fragment of epratuzumab (Figure 1b) , suggesting that the binding of this antibody ... Epratuzumab induces b1 integrin on CD27negative B- cells In order to analyse changes in the surface expression of b1 integrin on B- cell sub-populations (CD27negative B- cells and CD27positive B- cells) , ... CD27negative B- cells compared to CD27positive B- cells Epratuzumab binding to CD27negative B- cells induced a significant reduction of CD62L and b7 integrin surface expression, while b1 integrin was...

Ngày tải lên: 12/08/2014, 15:21

... of B cells, resembling the structure of germinal centers [11,17] These findings suggested that the chemokine CXCL13 is involved in B cell trafficking into the inflamed tissue and has a role in ... examined in Transwell™ inserts (Corning Costar) with a diameter of 6.5 mm and µm pores, by using fibronectin-precoated membranes as described previously [24] In brief, × 105 PBMC were suspended in ... streptavidin-FITC (for CXCR4) (0.5 µg/ml; Pharmingen), cells were washed twice in phosphate-buffered saline/2% BSA/4 mM EDTA To determine the frequency of CD5+ B cells, PBMC were stained as described...

Ngày tải lên: 09/08/2014, 06:23

... disease in human SLE is the increased percentages of activated B cells and plasma cells in peripheral blood [30-34], probably reflecting increased systemic numbers of activated B cells and plasma cells ... saturated in vivo with circulating BLyS, rendering them incapable of binding to BLyS in the in vitro detection assay We not yet know whether the antiBLyS autoantibodies are functionally neutralizing but, ... full-length BLyS or ∆BLyS mRNA levels compared with that between SLE disease activity and BLyS protein levels could not be attributable to interference by biologically inactive (inhibitory) ∆BLyS protein...

Ngày tải lên: 09/08/2014, 07:20

... Caspase 8L binds to the Fas-associating protein with death domain (FADD) and blocks the binding of caspase to FADD by competiive inhibition [39] Indeed, in hematopoietic stem cells and leukemic cells, ... Dassel, Germany) Nonspecific protein binding was blocked using PBS with 0.1% Tween 20 and 5% bovine serum albumin (Sigma) After incubation with anti-caspase (Cell Signaling Technology, Danvers, MA, ... placed in the upper chamber The lower chambers contained DMEM with recombinant VEGF, recombinant TNF-α (both from Preprotech) or DMEM only After incubation at 37°C for 90 minutes, migrated cells...

Ngày tải lên: 09/08/2014, 10:20

... RPMI 1640 medium (GibcoBRL, Carlsbad, CA, USA) containing penicillin (100 U/ml), streptomycin (100 μg/ ml), and 10% fetal bovine serum (GibcoBRL) that had been inactivated by heating to 56°C for ... mononuclear cells individuals (PBMCs), obtained from healthy individuals, were incubated with serum from patients with systemic lupus erythematosus (SLE) or healthy control individuals for 24 hours Interferon ... dendritic cells Blood 2007, 109:1165-1173 34 Vallin H, Blomberg S, Alm GV, Cederblad B, Ronnblom L: Patients with systemic lupus erythematosus (SLE) have a circulating inducer of interferon-alpha...

Ngày tải lên: 09/08/2014, 10:23

... followed by the addition of europium-labeled BLyS Anti-belimumab antibody present in the sample would bind to the immobilized belimumab, and competitively inhibit binding of europium-labeled BLyS ... in CD20+ B cells Changes in CD20+ B cells Median percentage change from baseline in CD20+ B cells in (a) single-dose cohorts and (b) double-dose cohorts Arrows indicate time of belimumab administration ... with high affinity The antibody exerts its biologic activity by preventing the binding of BLyS to its receptors [19] Belimumab potently inhibits BLyS-induced proliferation of B cells in vitro and...

Ngày tải lên: 09/08/2014, 13:21

... cells and B cells, evidence has been obtained that these Tregs also induce tolerogenic DCs in transplant tolerance [85] In SLE once the DC balance has been shifted back to tolerogenic predominance, ... Humrich J, Ebling FM, Hocher B, Humaljoki T, Neumayer H, Burmester GR, Hahn BH, Radbruch A, Hiepe F: Intravenous injection of a D1 protein of the Smith proteins postpones murine lupus and induces ... of systemic lupus erythematosus responder or accessory cells [57] this finding Consistent with this explanation, another group reported that SLE responder cells can be inhibited by Tregs in an...

Ngày tải lên: 09/08/2014, 13:22

... molecule-grabbing tegrin expression in systemic lupus erythematosus dendritic cells Dendritic cell-specific intercellular adhesion molecule-grabbing noninnonintegrin expression in systemic lupus erythematosus ... terminated by washing three times with ice-cold PBS, followed by staining as described below Zymosan uptake Fluorescently labeled and unlabeled zymosan was reconstituted in mM sodium azide/PBS ... different subsets of human DCs In addition to binding immunoglobulin-opsonized particles, ligation of Fcγ receptor II by nucleic acid-containing immune complexes can trigger IFNα production by plasmacytoid...

Ngày tải lên: 09/08/2014, 13:22

... remains high at million IU but HBV and HIV viral loads remain undetectable with a CD4 count of 274 (13%) His current creatinine is maintained between 0·13 and 0·18 mmol/L and prednisolone has been ... may contribute to this protective effect of HIV DCs can be infected by HIV, and TLR9 signalling-changes in pDC function may be reduced indirectly by HIV gp120 [14] Loss of pDC in HIV infection ... Sánchez-Tapias JM, Ingelmo M: Hepatitis C virus infection mimicking systemic lupus erythematosus: study of hepatitis C virus infection in a series of 134 Spanish patients with systemic lupus erythematosus...

Ngày tải lên: 11/08/2014, 17:21

... cell activity in vivo in an animal model as described by Hippen and colleagues [11] with human cord blood CD4+CD25+ cells Abbreviations IL-2 = interleukin-2; SLE = systemic lupus erythematosus; ... to distinguish CD4+Foxp3+ Tregs from non-Tregs would be to examine the stability of Foxp3 expressed by these cells in culture As stated above, Foxp3 expressed by Tregs is relatively stable whereas ... the cells being Tregs rather than T-effector cells These workers isolated CD4+CD25–CD127– cells by fluorescence-activated cell sorting and found that the cells contained up to 53% Foxp3+ T cells...

Ngày tải lên: 12/08/2014, 11:22

... of human B cells and their differentiation into antibody-producing cells [25] This observation provides a novel insight into understanding the pathogenic role for IL-17 in lupus because aberrant ... into antibody-producing cells [25] Yet, it remains unknown why IL-17 production is increased in lupus and whether such a finding is related to Th1 cells producing IFN-g Determining the balance ... nephritis Infiltrates of IL-17 producing T cells, including CD4 + and DN T cells, have been found in lupus nephritis In addition, IL17 gene expression was detected in T cells infiltrating the kidneys...

Ngày tải lên: 12/08/2014, 12:20

... not examine whether the BMDC changes were intrinsic defects or secondary to microenvironmental changes in the BM, including the cytokine milieu in our SLE patients This should be evaluated in future ... NC, normal control; SLE, systemic lupus erythematosus; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, ... dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin...

Ngày tải lên: 12/08/2014, 12:20

... following pDCs' interaction with apoptotic cells Abbreviations AC-BMDCs: apoptotic cell-pulsed bone marrow-derived DCs; APCs: antigen presenting cells; DCs: dendritic cells; IFN: interferon; IL, interleukin; ... activity in systemic lupus erythematosus Clin Rheumatol 2005, 24:107-110 Graham KLU, Paul J: Sources of autoantigens in systemic lupus erythematosus Current Opinion in Rheumatology 2005, 17:513-517 Boule ... changes in IL-10 protein levels in the supernatant of the SLE pDCs + apoPMNs cultures These findings are interesting as DC IL-18 is involved in the induction of Th1 response and IL-10 induces...

Ngày tải lên: 12/08/2014, 14:22

... hour After incubation, the non-binding Jurkat cells were removed by repeated washing with cold PBS The remaining firmly adherent cells were trypsinized (0.05% tripsin, Hyclone, Beijing, China), harvested ... binding/ingestion in PBS pretreated wellpercentage of binding/ingestion in IgG pretreated well)/ percentage of binding/ingestion in PBS pretreated well × 100 Statistical analysis For continuous ... times with PBS plus 0.05% Tween 20 and blocked with PBS containing 0.3% bovine serum albumin for two hours at 37°C The blocking buffer was tapped off and washed as described above Table Demographic...

Ngày tải lên: 12/08/2014, 15:22