b bmp signalling pathway component and target genes in oa bone
Báo cáo y học: "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling" pps
Ngày tải lên :
09/08/2014, 10:21
... differences in safranin O staining intensity between BMP- 2-exposed cartilage and controls were observed by mere visual investigation Therefore, the safranin O staining intensity was scored in patellar and ... signalling of BMP- 2, but also that of other BMPs Therefore, in cases in which gremlin was used, BMP instead of BMP- 2 was mentioned Histology Knee joints of mice were isolated and fixed for days in ... BMP- 2 is able to induce chondrogenesis in human mesenchymal stem cells in culture [9] For cartilage reparative reasons, BMP- 2 can be used to induce chondrogenesis by coating a scaffold with BMP- 2...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
Ngày tải lên :
02/11/2012, 11:17
... TRAF1 and TRAF2 [31] Therefore, those proteins might be coprecipitated with cIAP2 in our experiments The band located beyond cIAP2 probably was the 73 KD big TNFR2 The two bands below the cIAP2 band ... examined with RT-PCR and Northern blot as described in Figure A RT-PCR detects XIAP B Northern blot detects XIAP β-Actin was used as a loading control as well 575bp 1.9Kb XIAP β-Actin 4.0Kb 1 ... expression in the persistently HBV expressing cell line HepG2.215 and its parent cell line, a non-HBV expressing cell line HepG2 We found that cIAP1 and cIAP2 were clearly increased in the HBV expressing...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
Ngày tải lên :
15/02/2014, 04:20
... expression in one cell line than any other line (Table 1) The microarray results were deposited in the CIBEX microarray database (accession CBX 100) Quantification of 19 candidate genes by qPCR Using ... adhesion by IPA The connection including TGM2, IL 1B, and PLAU and the connection including RAB25, SNAI1, and CDH1 were suggested to be related to tumor invasion by IPA STMN1 was suggested to influence ... protein-protein binding; RB, regulation of binding) gene expression was not fully consistent among the subtypes, PCA with the present 12 genes could be used effectively to classify the four subtypes...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
Ngày tải lên :
18/02/2014, 04:20
... vector bearing the potential miR-23a binding site in the fluorescent reporter, and found an increase in EGFP intensity after blocking miR-23a and a decrease in EGFP intensity after overexpressing ... and transfection Human gastric adenocarcinoma cell line MGC803 was maintained in RPMI1640 (GIBCO BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, 100 IUÆmL)1 of penicillin ... triplicate Bioinformatic method The miRNA targets predicted by computer-aided algorithms were obtained from PicTar (the Rajewsky Lab, Berlin, Germany http://pictar.mdc-berlin.de/cgi-bin/new_PicTar_...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
Ngày tải lên :
19/02/2014, 05:20
... HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and HepG2 ... cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma) YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium (Sigma), and K562, KG1 and Jurkat cells were maintained in RPMI-1640 ... (A) and HepG2 cells (C) by northern blot analysis HO-1 and HO-2 proteins were determined in HeLa cells (B) and HepG2 cells (D) by western blot analysis The data are mean ± SEM from three independent...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
Ngày tải lên :
19/02/2014, 06:20
... was reused for a-tubulin monoclonal antibody Note that HO-1 mRNA and protein were undetectable in Jurkat and K562 cells under the conditions used bone marrow origin, including KG1 myeloid cells, ... levels of HO-1 and HO-2 proteins by western blot analysis (Fig 1B) The expression levels of HO-1 protein remained unchanged in KG1 cells and decreased by about 20% in YN-1 cells after FEBS Journal ... level, HO-1 protein was undetectable in Jurkat and K562 cells The HO-2 protein levels were noticeably decreased under hypoxia in Jurkat, K562 and YN-1 cells, in which a-tubulin protein levels were...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... as determined by the increase in mean fluorescence intensity (MFI) (Fig 1C) As shown in Fig 1B, permeabilization with saponin did not increase background staining when rabbit immunoglobulins were ... the plasma membrane was examined by Annexin V binding Briefly, cells were washed twice with binding buffer (10 mm Hepes, 140 mm NaCl and 2.5 mm CaCl2, pH 7.4) and incubated with Annexin V-FITC for ... in iron-rich media We have also shown that the increase in the prooxidant state caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... the number of basic residues of lysine, arginine and histidine, could occur However it seemed probable that the eEF1A isoforms could act as a controlling event in maintaining the viability and/ or ... and methods As a loading control, the presence of b- actin protein was also confirmed by using specific antibody, as described in the Materials and methods b- actin, used as a loading control, occurred ... striking relationship between the growth-inhibition effect exerted by cytotoxic GT oligomers and their selective binding to nuclear eEF1A In fact, in normal human lymphocytes no appreciable binding...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
Ngày tải lên :
21/02/2014, 00:20
... than that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It ... differently treated and transfected cells (A) Expression of PKB protein and b- actin (loading control) in differently treated and transfected cells (Western blot) (B) PKB activity in insulin and LY294002 ... cells (C) PKB activity in sense or antisense PKB transfected cells before and after insulin treatment UnC, Mock, UnC + Ins or Ins, Mock + Ins, S-PKB, S-PKB + Ins, AS-PKB, AS-PKB + Ins, LY294002,...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
Ngày tải lên :
07/03/2014, 12:20
... listed below before the addition of 10 lM BrdU BrdU incorporation was determined by chemiluminescence as described in Experimental procedures, following incubation for 12 h Proliferation in the absence ... inhibitory effect exerted by ATP on BrdU incorporation The inhibition of proliferation of LXF-289 cells by 100 lM ATP (see value in Table 1) is taken as 100% (B) Basal incorporation of BrdU into ... lm SB203580) or phosphatidylinositol-3-kinase (PI3K) (wortmannin), indicating that each pathway seems to be involved in ATP-mediated inhibition of LXF-289 cell proliferation (Table 2) Inhibition...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
Ngày tải lên :
07/03/2014, 15:20
... chemotherapy Indeed, it has been shown that a palindromic trioctamer of this sequence can interfere with CREB binding, and specifically inhibit PKA subunit expression, interfering with the CRE-PKA pathway ... reduces cyclin D1 and cyclin-dependent kinase (CDK) levels and retinoblastoma protein (Rb) phosphorylation CDO-induced growth inhibition was independent of p53 status [18,19], and accompanied by the ... changes in the levels of CDK 4, cyclin D or CDK in response to drug combinations Only cyclin B appeared to be affected, its level being reduced in combination cultures (Fig 6B) This observation...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
Ngày tải lên :
07/03/2014, 16:20
... with blocking buffer [3% (w/v) bovine serum albumin/10% (v/v) goat serum in NaCl/Pi] for 30 and incubated with anti-BrdU (Sigma B2 531, : 150) in blocking buffer, overnight in a wet chamber Cells ... geminin is expressed in S and G2 phases, when licensing should be inhibited and when the Cdt1 protein is undetectable [13,16] Based on these findings, one might have expected geminin to be induced ... differences in relative amounts of Cdt1 and its inhibitor in different cell lines It would be interesting to investigate whether geminin and/ or Cdt1 levels or the ratio of Cdt1 to its inhibitor geminin...
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Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
Ngày tải lên :
08/03/2014, 16:20
... be from contamination of the PKA preparation because this band was not detectable with Western blot analysis (WB in Fig 3B) and was seen with CBB staining only when PKA was added (CBB panel in ... images visualized by Coomassie Brilliant Blue staining (CBB), by Western blotting using anti-TPH serum (WB), and by autoradiography (32P) PKA was first examined using its catalytic subunit for phosphorylation ... have been digested away in the absence of proteasome inhibitors (lane in Fig 1B, 32P vs WB) Inhibition of TPH degradation in the cell-free proteasome system by protein kinase inhibitors We examined...
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Báo cáo Y học: The SK-N-MC cell line expresses an orexin binding site different from recombinant orexin 1-type receptor pptx
Ngày tải lên :
08/03/2014, 22:20
... experiments drugs Ó FEBS 2002 SK-N-MC cell line expresses different orexin binding sites (Eur J Biochem 269) 1131 Table Binding affinity of h-orexin A and B, C-terminal orexin A and B fragments and reported ... of ligands, e.g EXBN 8016BS or orexin A, to inhibit either 125I-labelled orexin A or Ó FEBS 2002 1134 H A Wieland et al (Eur J Biochem 269) 125 I-labelled orexin B binding at the recombinant ... food intake Sensitivity of orexin A binding to NPY has been described earlier by studying 125I-labelled orexin A binding [30] Interestingly some affinity of NPY could be identified for orexin receptors...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
Ngày tải lên :
16/03/2014, 06:20
... titration curve Papain and cathepsin B inhibition The cysteine protease inhibitory capacity in cell lysates was determined by measuring the inhibition of papain as well as cathepsin B To measure the ... densitometric scanning of the western blot bands A B In uences of preincubation by non-labelled inhibitor In order to address the uptake mechanism, Capan-1 cells were incubated with labelled cystatin C after ... and end of a 30-min incubation, as above To measure the inhibition of cathepsin B (kindly provided by J Mort, Shriners Hospital, Montreal, Quebec, Canada), 83 lL buffer mix as above, containing...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
Ngày tải lên :
16/03/2014, 13:20
... nucleolin [27] could be a major contaminant of eEF1A protein can be excluded by MALDI TOF analysis of the Coomassie blue band extract [21] and by the absence of a corresponding nucleolin signal in ... 1711–1716 17 Dapas B, Perissin L, Pucillo C, Quadrifoglio F & Scaggiante B (2002) Increase in therapeutic index of doxorubicin and vinblastine by aptameric oligonucleotide in human T lymphoblastic drug-sensitive ... After 30 incubation at room temperature, the samples were crosslinked by exposure to UV light for 10 min, denatured by adding SDS ⁄ PAGE loading buffer and boiling The samples were loaded onto...
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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
Ngày tải lên :
16/03/2014, 16:20
... oligomannosides bound to glycoproteins after 20 of preincubation at 0.175 mM Glc followed by h of labeling and a h chase in culture medium containing mM Glc and mM Man Under these conditions as described ... proportion of Man5 species bound to the glycoprotein fraction (Fig 4D) Glycoproteins bearing only Man9 species can be obtained in two ways: (a) induction of UPR by extensive preincubation at low Glc concentration ... glycoproteins These different proportions were obtained by using different preincubation times in 0.175 mM Glc before the 1-h pulse (from 120 preincubation for 0% Man5 species to 20 preincubation...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... confirmed by indirect measurement of ROS obtained by comparing aconitase activity in standard conditions and after 18 h of incubation with PBN (Fig 7) In biological systems PBN [60,61], or N-t-butyl ... spin-trapping N-tert-butyl-a-phenylnitrone (PBN) [47,48] Figure shows a trend of increasing aconitase activity in almost all PBN-treated cell lines The increase was most marked in bone q+ and ... in three tumour cell lines derived from bone (osteosarcoma, 14 3B) , muscle (rhabdomyosarcoma, RD) and lung (adenocarcinoma, A549) and in the respective q0 cells: 143Bq0 (bone) , RDq0 (muscle) and...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
Ngày tải lên :
17/03/2014, 23:20
... of the IL-2Rb chain in transformed human NK and fibroblast cells Thus, to better understand the role of lipid rafts in cell growth/ viability-signaling of T cells, in general, and in the unregulated ... Cooperative interactions between the interleukin receptor alpha and beta chains alter the interleukin 2-binding affinity of the receptor subunits Proc Natl Acad Sci USA 91, 3344–3347 Marmor, M.D., Bachmann, ... distinct role of lipid rafts in regulating IL-2 signaling, namely sequestration of CD25 by lipid rafts impeding interaction with the IL-2Rb and c chains The observed difference between these and...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
Ngày tải lên :
22/03/2014, 21:20
... [6], and miR-15 and miR-16 simulate apoptosis in chronic lymphocytic leukemia by targeting BCL2 [7] On the other hand, miR-1 0b initiates breast cancer invasion and metastasis by repressing homeobox ... miR-22 could regulate ERa expression by directly binding to ERa 3¢-UTR, and inhibited ERa expression through both destabilizing mRNA and inhibiting translation FEBS Journal 277 (2010) 1684–1694 ª ... using a box plot The line inside each box is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate...
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